{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T10:45:12Z","timestamp":1782643512726,"version":"3.54.5"},"reference-count":54,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31602204"],"award-info":[{"award-number":["31602204"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"crossref","award":["2452017078"],"award-info":[{"award-number":["2452017078"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007548","name":"Northwest A & F University","doi-asserted-by":"publisher","award":["Z111021510"],"award-info":[{"award-number":["Z111021510"]}],"id":[{"id":"10.13039\/501100007548","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1016\/j.fsi.2017.10.012","type":"journal-article","created":{"date-parts":[[2017,10,12]],"date-time":"2017-10-12T15:16:58Z","timestamp":1507821418000},"page":"191-201","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":39,"special_numbering":"C","title":["Single-walled carbon nanotubes as delivery vehicles enhance the immunoprotective effect of a DNA vaccine against spring viremia of carp virus in common carp"],"prefix":"10.1016","volume":"71","author":[{"given":"Chen","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Wei","family":"Zha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gao-Xue","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2017.10.012_bib1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.jviromet.2015.12.008","article-title":"An N-targeting real-time PCR strategy for the accurate detection of spring viremia of carp virus","volume":"229","author":"Shao","year":"2016","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.fsi.2017.10.012_bib2","first-page":"125","article-title":"Isolation of the viral causative agent from the acute form of infectious dropsy of carp","volume":"41","author":"Fijan","year":"1971","journal-title":"Vet. Arh."},{"key":"10.1016\/j.fsi.2017.10.012_bib3","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1577\/1548-8667(2002)014<0161:FROSVO>2.0.CO;2","article-title":"First report of spring viremia of carp virus (SVCV) in North America","volume":"14","author":"Goodwin","year":"2002","journal-title":"J. Aquat. Anim. Health"},{"key":"10.1016\/j.fsi.2017.10.012_bib4","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1577\/H03-064.1","article-title":"First report of spring viremia of carp virus (SVCV) in wild common carp in North America","volume":"16","author":"Dikkeboom","year":"2004","journal-title":"J. Aqua Anim. Health"},{"key":"10.1016\/j.fsi.2017.10.012_bib5","first-page":"194","article-title":"Isolation of spring viraemia of carp virus (SVCV) from cultured Koi (Cyprinus carpio koi) and common carp (Cyprinus carpio carpio) in PR China","volume":"24","author":"Liu","year":"2004","journal-title":"Bull. Eur. Assn Fish. P"},{"key":"10.1016\/j.fsi.2017.10.012_bib6","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1007\/s00705-007-0971-8","article-title":"Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China","volume":"152","author":"Teng","year":"2007","journal-title":"Arch Virol"},{"key":"10.1016\/j.fsi.2017.10.012_bib7","first-page":"233","article-title":"Vaccination of fish in European pond culture: prospects and constraints","volume":"23","author":"Fijan","year":"1984","journal-title":"Sym Bio Hung"},{"key":"10.1016\/j.fsi.2017.10.012_bib8","series-title":"Rhabdovirus Carpio in Herpivorous Fishes: Isolation, Pathology and Comparative Susceptibility of Fishes","first-page":"333","author":"Shchelkunov","year":"1989"},{"key":"10.1016\/j.fsi.2017.10.012_bib9","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1007\/s00705-007-0971-8","article-title":"Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China","volume":"152","author":"Teng","year":"2007","journal-title":"Arch. Virol."},{"key":"10.1016\/j.fsi.2017.10.012_bib10","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1051\/vetres\/2010022","article-title":"Emerging viral diseases of fish and shrimp","volume":"41","author":"Walker","year":"2010","journal-title":"Vet. Res."},{"key":"10.1016\/j.fsi.2017.10.012_bib11","doi-asserted-by":"crossref","first-page":"261","DOI":"10.3354\/dao052261","article-title":"Spring viremia of carp (SVC)","volume":"52","author":"Ahne","year":"2002","journal-title":"Dis. Aquat. Organ"},{"key":"10.1016\/j.fsi.2017.10.012_bib12","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1099\/jgv.0.000436","article-title":"Spring viraemia of carp virus: recent advances","volume":"97","author":"Ashraf","year":"2016","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.fsi.2017.10.012_bib13","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1577\/1548-8667(2000)012<0167:FVORTA>2.0.CO;2","article-title":"DNA vaccination of rainbow trout against viral hemorrhagic septicemia virus: a dose-response and time-course study","volume":"12","author":"Lorenzen","year":"2000","journal-title":"J. Aquat. Anim. Health"},{"key":"10.1016\/j.fsi.2017.10.012_bib14","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1006\/fsim.2000.0286","article-title":"Fish DNA vaccine against infectious hematopoietic necrosis virus: efficacy of various routes of immunisation","volume":"10","author":"Corbeil","year":"2000","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.10.012_bib15","doi-asserted-by":"crossref","first-page":"6415","DOI":"10.1016\/j.vaccine.2008.08.071","article-title":"DNA vaccine protects ornamental koi (Cyprinus carpio koi) against North American spring viremia of carp virus","volume":"26","author":"Emmenegger","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.10.012_bib16","doi-asserted-by":"crossref","first-page":"4927","DOI":"10.1016\/j.vaccine.2006.03.062","article-title":"DNA vaccination can protect Cyprinus Carpio against spring viraemia of carp virus","volume":"24","author":"Kanellos","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.10.012_bib17","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1586\/14760584.5.6.803","article-title":"Optimization and delivery of plasmid DNA for vaccination","volume":"5","author":"Jechlinger","year":"2014","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.fsi.2017.10.012_bib18","first-page":"79","article-title":"Technical aspects of the administration of vaccines","volume":"90","author":"Horne","year":"1997","journal-title":"Dev. Biol. Stand"},{"key":"10.1016\/j.fsi.2017.10.012_bib19","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/j.dci.2011.03.007","article-title":"Advances in fish vaccine delivery","volume":"35","author":"Plant","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.10.012_bib20","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1006\/fsim.1996.0033","article-title":"The uptake of bovine serum albumin by the skin of bath-immunised rainbow trout Oncorhynchus mykiss","volume":"6","author":"Ototake","year":"1996","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.10.012_bib21","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.vaccine.2003.12.036","article-title":"On the road to a tumor cell vaccine: 20 years of cellular immunotherapy","volume":"23","author":"Yannelli","year":"2004","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.10.012_bib22","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1002\/adma.201104763","article-title":"Mesoporous silica nanoparticles: synthesis, biocompatibility and drug delivery","volume":"24","author":"Tang","year":"2012","journal-title":"Adv. Mater"},{"key":"10.1016\/j.fsi.2017.10.012_bib23","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1039\/b410943k","article-title":"Biomedical applications of functionalised carbon nanotubes","volume":"5","author":"Bianco","year":"2005","journal-title":"Chem. Commun."},{"key":"10.1016\/j.fsi.2017.10.012_bib24","doi-asserted-by":"crossref","first-page":"1170","DOI":"10.1038\/cr.2010.133","article-title":"Multi-walled carbon nanotubes conjugated to tumor protein enhance the uptake of tumor antigens by human dendritic cells in vitro","volume":"20","author":"Sun","year":"2010","journal-title":"Cell Res."},{"key":"10.1016\/j.fsi.2017.10.012_bib25","doi-asserted-by":"crossref","first-page":"145104","DOI":"10.1088\/0957-4484\/21\/14\/145104","article-title":"Subcutaneous injection of water-soluble multi-walled carbon nanotubes in tumor-bearing mice boosts the host immune activity","volume":"21","author":"Meng","year":"2010","journal-title":"Nanotechnol"},{"key":"10.1016\/j.fsi.2017.10.012_bib26","doi-asserted-by":"crossref","first-page":"5475","DOI":"10.1021\/nl301865c","article-title":"Nanotube on-command intracellular drug delivery","volume":"12","author":"Wu","year":"2012","journal-title":"Nano Lett."},{"key":"10.1016\/j.fsi.2017.10.012_bib27","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1038\/nnano.2007.347","article-title":"Direct imaging of single-walled carbon nanotubes in cells","volume":"2","author":"Porter","year":"2007","journal-title":"Nat. Nanotechnol."},{"key":"10.1016\/j.fsi.2017.10.012_bib28","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1038\/nnano.2006.209","article-title":"Cellular uptake of functionalized carbon nanotubes is independent of functional group and cell type","volume":"2","author":"Kostarelos","year":"2007","journal-title":"Nat. Nanotechnol."},{"key":"10.1016\/j.fsi.2017.10.012_bib29","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.fsi.2014.11.026","article-title":"Protective immunity of grass carp immunized with DNA vaccine encoding the vp7 gene of grass carp reovirus using carbon nanotubes as a carrier molecule","volume":"42","author":"Zhu","year":"2015","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.10.012_bib30","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1577\/1548-8667(1989)001<0284:EDOIHN>2.3.CO;2","article-title":"Enhanced detection of infectious hematopoietic necrosis virus and other fish viruses by pretreatment of cell monolayers with polyethylene glycol","volume":"1","author":"Batts","year":"1989","journal-title":"J. Aquat. Anim. Health"},{"key":"10.1016\/j.fsi.2017.10.012_bib31","first-page":"3050","article-title":"Amino acid functionalisation of water soluble carbon nanotubes","volume":"8","author":"Georgakilas","year":"2003","journal-title":"Chem. Commun."},{"key":"10.1016\/j.fsi.2017.10.012_bib32","doi-asserted-by":"crossref","first-page":"5242","DOI":"10.1002\/anie.200460437","article-title":"Functionalized carbon nanotubes for plasmid DNA gene delivery","volume":"43","author":"Pantarotto","year":"2004","journal-title":"Angew. Chem."},{"key":"10.1016\/j.fsi.2017.10.012_bib33","doi-asserted-by":"crossref","first-page":"3870","DOI":"10.1016\/j.vaccine.2009.04.012","article-title":"The protective mechanisms induced by a fish rhabdovirus DNA vaccine depend on temperature","volume":"27","author":"Lorenzen","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.10.012_bib34","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1016\/j.vaccine.2005.07.109","article-title":"Protection of flounder against hirame rhabdovirus (HIRRV) with a DNA vaccine containing the glycoprotein gene","volume":"24","author":"Seo","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.10.012_bib35","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.vetmic.2011.12.025","article-title":"The antiviral defense mechanisms in mandarin fish induced by DNA vaccination against a rhabdovirus","volume":"157","author":"Chen","year":"2012","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.fsi.2017.10.012_bib36","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.fsi.2014.09.014","article-title":"Single-walled carbon nanotubes as candidate recombinant subunit vaccine carrier for immunization of grass carp against grass carp reovirus","volume":"41","author":"Zhu","year":"2014","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.10.012_bib37","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.dci.2013.01.014","article-title":"Gene expression profiling in live attenuated Edwardsiella tarda vaccine immunized and challenged zebrafish: insights into the basic mechanisms of protection seen in immunized fish","volume":"40","author":"Yang","year":"2013","journal-title":"Dev comp immunol"},{"key":"10.1016\/j.fsi.2017.10.012_bib38","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/0165-2427(94)05430-Z","article-title":"Natural hemolytic and bactericidal activities of sea bream Sparus aurata serum are effected by the alternative complement pathway","volume":"45","author":"Sunyer","year":"1995","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.fsi.2017.10.012_bib39","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.fsi.2007.11.011","article-title":"Dietary sodium alginate administration affects fingerling growth and resistance to Streptococcus sp and iridovirus, and juvenile non-specific immune responses of the orange-spotted grouper, Epinephelus coioides","volume":"25","author":"Yeh","year":"2008","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.10.012_bib40","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.jviromet.2008.05.031","article-title":"Development of a sensitive and quantitative assay for spring viremia of carp virus based on real-time RT-PCR","volume":"152","author":"Yue","year":"2008","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.fsi.2017.10.012_bib41","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2017.10.012_bib42","first-page":"447","article-title":"Potency testing of fish vaccines","volume":"49","author":"Amend","year":"1981","journal-title":"Dev. Biol. Stand"},{"key":"10.1016\/j.fsi.2017.10.012_bib43","doi-asserted-by":"crossref","first-page":"6282","DOI":"10.1016\/j.vaccine.2006.05.073","article-title":"Immune response against grouper nervous virus by vaccination of virus-like particles","volume":"24","author":"Liu","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.10.012_bib44","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/j.vaccine.2007.11.065","article-title":"Development of an oral vaccine for immunisation of rainbow trout (Oncorhynchus mykiss) against viral haemorrhagic septicaemia","volume":"26","author":"Life","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.10.012_bib45","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/j.vaccine.2016.12.055","article-title":"Immunogenicity differences of a 15-valent pneumococcal polysaccharide conjugate vaccine (PCV15) based on vaccine dose, route of immunization and mouse strain","volume":"35","author":"Caroaguilar","year":"2017","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2017.10.012_bib46","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1016\/j.fsi.2013.05.029","article-title":"Status and future perspectives of vaccines for industrialised fin-fish farming","volume":"35","author":"Brudeseth","year":"2013","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.10.012_bib47","doi-asserted-by":"crossref","first-page":"4635","DOI":"10.4049\/jimmunol.164.9.4635","article-title":"Increased DNA vaccine delivery and immunogenicity by electroporation in vivo","volume":"164","author":"Widera","year":"2000","journal-title":"J. Immunol."},{"key":"10.1016\/j.fsi.2017.10.012_bib48","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.fsi.2010.09.016","article-title":"Poly(lactic-co-glycolic acid) nanoparticles as candidate DNA vaccine carrier for oral immunization of Japanese flounder (Paralichthys olivaceus) against lymphocystis disease virus","volume":"30","author":"Tian","year":"2011","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.10.012_bib49","doi-asserted-by":"crossref","first-page":"4782","DOI":"10.1002\/anie.200500042","article-title":"Polyethylenimine-grafted multiwalled carbon nanotubes for secure noncovalent immobilization and efficient delivery of DNA","volume":"44","author":"Liu","year":"2005","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"10.1016\/j.fsi.2017.10.012_bib50","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.aquaculture.2013.11.017","article-title":"Delivery of DNA vaccine using chitosan-tripolyphosphate (CS\/TPP) nanoparticles in Asian sea bass, Lates calcarifer (Bloch, 1790) for protection against nodavirus infection","volume":"420\u2013421","author":"Vimal","year":"2014","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2017.10.012_bib51","doi-asserted-by":"crossref","first-page":"5300","DOI":"10.1021\/nn200182x","article-title":"Single-walled carbon nanotubes deliver peptide antigen into dendritic cells and enhance IgG responses to tumor-associated antigens","volume":"5","author":"Villa","year":"2011","journal-title":"ACS Nano"},{"key":"10.1016\/j.fsi.2017.10.012_bib52","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1016\/j.fsi.2008.01.014","article-title":"Chronic exposure to the parasite Enteromyxum leei (Myxozoa: Myxosporea) modulates the immune response and the expression of growth, redox and immune relevant genes in gilthead sea bream, Sparus aurata L","volume":"24","author":"Sitj\u00e0-Bobadilla","year":"2008","journal-title":"Fish. Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.10.012_bib53","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/j.dci.2007.09.009","article-title":"Characterization and functional analysis of goldfish (Carassius auratus L.) tumor necrosis factor-alpha","volume":"32","author":"Grayfer","year":"2008","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.10.012_bib54","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1016\/j.fsi.2012.12.011","article-title":"Immune responses and immune-related gene expression profile in orange-spotted grouper after immunization with Cryptocaryon irritans vaccine","volume":"34","author":"Dan","year":"2013","journal-title":"Fish. Shellfish Immunol."}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464817306125?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464817306125?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T05:59:01Z","timestamp":1589781541000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464817306125"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12]]},"references-count":54,"alternative-id":["S1050464817306125"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2017.10.012","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2017,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Single-walled carbon nanotubes as delivery vehicles enhance the immunoprotective effect of a DNA vaccine against spring viremia of carp virus in common carp","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2017.10.012","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}