{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:20:34Z","timestamp":1762507234000,"version":"3.37.3"},"reference-count":34,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31572644"],"award-info":[{"award-number":["31572644"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, China","award":["2016LMFS-A25"],"award-info":[{"award-number":["2016LMFS-A25"]}]},{"name":"Taishan Scholar Climbing Project of Shandong Province"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2018,1]]},"DOI":"10.1016\/j.fsi.2017.11.017","type":"journal-article","created":{"date-parts":[[2017,11,13]],"date-time":"2017-11-13T08:46:26Z","timestamp":1510562786000},"page":"658-669","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"special_numbering":"C","title":["Identification and expression of complement component C8\u03b1, C8\u03b2 and C8\u03b3 gene in half-smooth tongue sole (Cynoglossus semilaevis) and C8\u03b1 recombinant protein antibacterial activity analysis"],"prefix":"10.1016","volume":"72","author":[{"given":"Guang","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunji","family":"Xiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yadong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Bai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenxia","family":"Sha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2017.11.017_bib1","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1128\/IAI.41.2.563-569.1983","article-title":"Complement activation by polysaccharide of lipopolysaccharide: an important virulence determinant of salmonellae","volume":"41","author":"Liangtakasaki","year":"1983","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fsi.2017.11.017_bib2","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1056\/NEJM200104123441506","article-title":"Advances in immunology: complement","author":"Walport","year":"2001","journal-title":"Nejm"},{"key":"10.1016\/j.fsi.2017.11.017_bib3","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1213\/ANE.0b013e3182652b7d","article-title":"Complement activation and cardiac surgery: a novel target for improving outcomes","volume":"115","author":"Stahl","year":"2012","journal-title":"Anesth. Analgesia"},{"key":"10.1016\/j.fsi.2017.11.017_bib4","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.it.2008.11.003","article-title":"Complement activation and inhibition: a delicate balance","volume":"30","author":"Sj\u00f6berg","year":"2009","journal-title":"Trends Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib5","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1146\/annurev.bi.57.070188.001541","article-title":"Molecular organization and function of the complement system","volume":"57","author":"M\u00fcller-Eberhard","year":"2003","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.fsi.2017.11.017_bib6","first-page":"19","article-title":"Structure and function of C8 in the membrane attack sequence of complement","volume":"140","author":"Sodetz","year":"1988","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib7","doi-asserted-by":"crossref","first-page":"11997","DOI":"10.1016\/S0021-9258(19)70233-6","article-title":"The eighth component of human complement. Purification and physicochemical characterization of its unusual subunit structure","volume":"255","author":"Steckel","year":"1980","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fsi.2017.11.017_bib8","doi-asserted-by":"crossref","first-page":"5229","DOI":"10.1021\/bi00391a003","article-title":"The eighth component of human complement: evidence that it is an oligomeric serum protein assembled from products of three different genes","volume":"26","author":"Ng","year":"1987","journal-title":"Biochemistry"},{"key":"10.1016\/j.fsi.2017.11.017_bib9","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1146\/annurev.iy.06.040188.001113","article-title":"Structure, organization, and regulation of the complement genes","volume":"6","author":"Campbell","year":"1988","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib10","doi-asserted-by":"crossref","first-page":"4318","DOI":"10.1016\/S0021-9258(18)32625-5","article-title":"Evidence of direct insertion of terminal complement proteins into cell membrane bilayers during cytolysis. Labeling by a photosensitive membrane probe reveals a major role for the eighth and ninth components","volume":"258","author":"Steckel","year":"1983","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fsi.2017.11.017_bib11","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1016\/0161-5890(90)90001-G","article-title":"Localization and molecular modelling of the membrane-inserted domain of the ninth component of human complement and perforin","volume":"27","author":"Peitsch","year":"1990","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib12","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1126\/science.1147103","article-title":"Structure of C8\u03b1-MACPF reveals mechanism of membrane attack in complement immune defense","volume":"317","author":"Hadders","year":"2007","journal-title":"Science"},{"key":"10.1016\/j.fsi.2017.11.017_bib13","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1016\/j.molimm.2006.03.012","article-title":"Incorporation of human complement C8 into the membrane attack complex is mediated by a binding site located within the C8beta MACPF domain","volume":"44","author":"Brannen","year":"2007","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib14","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s00894-008-0412-y","article-title":"A disulfide linked model of the complement protein C8 gamma complexed with C8 alpha indel peptide","volume":"15","author":"Stavrakoudis","year":"2009","journal-title":"J. Mol. Model."},{"key":"10.1016\/j.fsi.2017.11.017_bib15","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1016\/j.molimm.2007.06.359","article-title":"Crystal structure of complement protein C8\u03b3 in complex with a peptide containing the C8\u03b3 binding site on C8\u03b1: implications for C8\u03b3 ligand binding","volume":"45","author":"Lovelace","year":"2008","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib16","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/S0167-4838(00)00155-2","article-title":"Human complement protein C8 gamma","volume":"1482","author":"Schreck","year":"2000","journal-title":"Biochimica biophysica acta"},{"key":"10.1016\/j.fsi.2017.11.017_bib17","doi-asserted-by":"crossref","first-page":"5290","DOI":"10.1021\/bi0601860","article-title":"Functional studies of the MACPF domain of human complement protein C8 alpha reveal sites for simultaneous binding of C8 beta, C8 gamma, and C9","volume":"45","author":"Slade","year":"2006","journal-title":"Biochemistry"},{"key":"10.1016\/j.fsi.2017.11.017_bib18","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1006\/fsim.1999.0252","article-title":"Complement system of bony and cartilaginous fish","volume":"10","author":"Nonaka","year":"2000","journal-title":"Fish shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib19","first-page":"707","article-title":"Genomic characterization and expression analysis of complement component 8\u03b1 and 8\u03b2 in rock bream (Oplegnathus fasciatus)","volume":"33","author":"Wickramaarachchi","year":"2012","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib20","doi-asserted-by":"crossref","first-page":"2188","DOI":"10.1016\/j.molimm.2006.01.004","article-title":"Cloning and phylogenetic analysis of the alpha subunit of the eighth complement component (C8) in rainbow trout","volume":"43","author":"Papanastasiou","year":"2006","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib21","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0145-305X(02)00092-7","article-title":"Molecular cloning of the \u03b2 subunit of complement component eight of rainbow trout","volume":"27","author":"Kazantzi","year":"2003","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib22","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.dci.2005.06.023","article-title":"The gamma subunit of the eighth complement component (C8) in rainbow trout","volume":"30","author":"Papanastasiou","year":"2006","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib23","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/S0161-5890(96)00053-3","article-title":"Terminal components of carp complement constituting a membrane attack complex","volume":"33","author":"Nakao","year":"1996","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib24","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/S0161-5890(96)00054-5","article-title":"Purification and characterization of the eighth and ninth components of carp complement","volume":"33","author":"Uemura","year":"1996","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib25","doi-asserted-by":"crossref","first-page":"345","DOI":"10.3390\/ijms17030345","article-title":"Molecular characterization and expression analyses of the complement component C8\u03b1,C8\u03b2 and C9 genes in Yellow Catfish (Pelteobagrus fulvidraco) after the Aeromonas hydrophila challenge","volume":"17","author":"Zheng","year":"2016","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fsi.2017.11.017_bib26","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.fsi.2014.11.018","article-title":"Transcriptome analysis revealed changes of multiple genes involved in immunity in Cynoglossus semilaevis during Vibrio anguillarum infection","volume":"43","author":"Zhang","year":"2015","journal-title":"Fish shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib27","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1016\/j.fsi.2012.01.010","article-title":"Identification and expression analysis of goose-type lysozyme in half-smooth tongue sole (Cynoglossus semilaevis)","volume":"32","author":"Sha","year":"2012","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib28","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.gene.2015.01.018","article-title":"Molecular cloning, characterization and expression analysis of complement component C8 beta in the orange-spotted grouper (Epinephelus coioides) after the Vibrio alginolyticus challenge","volume":"558","author":"Luo","year":"2015","journal-title":"Gene"},{"key":"10.1016\/j.fsi.2017.11.017_bib29","doi-asserted-by":"crossref","first-page":"R911","DOI":"10.1016\/S0960-9822(00)80102-5","article-title":"Chlamydial homologues of the MACPF (MAC\/perforin) domain","volume":"9","author":"Ponting","year":"1999","journal-title":"Curr. Biol."},{"key":"10.1016\/j.fsi.2017.11.017_bib30","doi-asserted-by":"crossref","first-page":"7030","DOI":"10.1021\/bi025696i","article-title":"Crystal structure of human complement protein C8 gamma at 1.2 A resolution reveals a lipocalin fold and a distinct ligand binding site","volume":"41","author":"Ortlund","year":"2002","journal-title":"Biochemistry"},{"key":"10.1016\/j.fsi.2017.11.017_bib31","first-page":"11","article-title":"Structure and function of perforin","volume":"140","author":"Podack","year":"1989","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib32","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1002\/hep.22034","article-title":"An organ with predominant innate immunity","volume":"47","author":"Gao","year":"2008","journal-title":"Hepatology"},{"key":"10.1016\/j.fsi.2017.11.017_bib33","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1006\/fsim.2001.0408","article-title":"The complement system in teleosts","volume":"12","author":"Holland","year":"2002","journal-title":"Fish shellfish Immunol."},{"key":"10.1016\/j.fsi.2017.11.017_bib34","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1006\/fsim.2001.0360","article-title":"Survival of Flavobacterium psychrophilum in rainbow trout (Oncorhynchus mykiss) serum in vitro","volume":"12","author":"Wiklund","year":"2002","journal-title":"Fish shellfish Immunol."}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464817306940?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464817306940?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,8,7]],"date-time":"2022-08-07T04:16:05Z","timestamp":1659845765000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464817306940"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1]]},"references-count":34,"alternative-id":["S1050464817306940"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2017.11.017","relation":{},"ISSN":["1050-4648"],"issn-type":[{"type":"print","value":"1050-4648"}],"subject":[],"published":{"date-parts":[[2018,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification and expression of complement component C8\u03b1, C8\u03b2 and C8\u03b3 gene in half-smooth tongue sole (Cynoglossus semilaevis) and C8\u03b1 recombinant protein antibacterial activity analysis","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2017.11.017","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}