{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T16:40:03Z","timestamp":1780677603171,"version":"3.54.1"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2018,3,19]],"date-time":"2018-03-19T00:00:00Z","timestamp":1521417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011102","name":"European Commission","doi-asserted-by":"publisher","award":["311993"],"award-info":[{"award-number":["311993"]}],"id":[{"id":"10.13039\/100011102","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006533","name":"Ministry of Agriculture of the Czech Republic","doi-asserted-by":"publisher","award":["MZE-RO0517"],"award-info":[{"award-number":["MZE-RO0517"]}],"id":[{"id":"10.13039\/501100006533","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2019,2]]},"DOI":"10.1016\/j.fsi.2018.03.028","type":"journal-article","created":{"date-parts":[[2018,3,19]],"date-time":"2018-03-19T14:18:13Z","timestamp":1521469093000},"page":"66-77","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":44,"special_numbering":"C","title":["Vaccination of carp against SVCV with an oral DNA vaccine or an insect cells-based subunit vaccine"],"prefix":"10.1016","volume":"85","author":[{"given":"C.W.E.","family":"Embregts","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Rigaudeau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Tacchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.P.","family":"Pijlman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Kampers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Vesel\u00fd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Pokorov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Boudinot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.F.","family":"Wiegertjes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Forlenza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2018.03.028_bib1","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.dci.2017.05.004","article-title":"Oral DNA vaccines based on CS-TPP nanoparticles and alginate microparticles confer high protection against infectious pancreatic necrosis virus (IPNV) infection in trout","volume":"74","author":"Ahmadivand","year":"2017","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib2","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/0165-2427(86)90144-3","article-title":"The influence of environmental temperature and infection route on the immune response of carp (Cyprinus carpio) to spring viremia of carp virus (SVCV)","volume":"12","author":"Ahne","year":"1986","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.fsi.2018.03.028_bib3","doi-asserted-by":"crossref","first-page":"261","DOI":"10.3354\/dao052261","article-title":"Spring viremia of carp (SVC)","volume":"52","author":"Ahne","year":"2002","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.fsi.2018.03.028_bib4","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/j.fsi.2015.05.045","article-title":"An oral DNA vaccine against infectious haematopoietic necrosis virus (IHNV) encapsulated in alginate microspheres induces dose-dependent immune responses and significant protection in rainbow trout (Oncorrhynchus mykiss)","volume":"45","author":"Ballesteros","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib5","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.fsi.2014.02.003","article-title":"Food pellets as an effective delivery method for a DNA vaccine against infectious pancreatic necrosis virus in rainbow trout (Oncorhynchus mykiss, Walbaum)","volume":"37","author":"Ballesteros","year":"2014","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib6","doi-asserted-by":"crossref","first-page":"201","DOI":"10.3354\/dao057201","article-title":"Emerging vesiculo-type virus infections of freshwater fishes in Europe","volume":"57","author":"Betts","year":"2003","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.fsi.2018.03.028_bib7","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.fsi.2005.12.001","article-title":"Improved uptake of plant-derived LTB-linked proteins in carp gut and induction of specific humoral immune responses upon infeed delivery","volume":"21","author":"Companjen","year":"2006","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.vaccine.2015.05.002","article-title":"Recombinant lactobacillus expressing G protein of spring viremia of carp virus (SVCV) combined with ORF81 protein of koi herpesvirus (KHV): a promising way to induce protective immunity against SVCV and KHV infection in cyprinid fish via oral vaccination","volume":"33","author":"Cui","year":"2015","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2018.03.028_bib9","first-page":"170","article-title":"Determination of the complete genomic sequence and analysis of the gene products of the virus of Spring Viremia of Carp, a fish rhabdovirus","volume":"21","author":"Dauber","year":"2001","journal-title":"Bull. Eur. Assoc. Fish Pathol."},{"key":"10.1016\/j.fsi.2018.03.028_bib10","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.fsi.2009.12.006","article-title":"Immunogenic and protective effects of an oral DNA vaccine against infectious pancreatic necrosis virus in fish","volume":"28","author":"de las Heras","year":"2010","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib11","series-title":"First DNA Vaccine in the EU Recommended for Use in Salmon [WWW Document]. 2016-04-22","author":"EMA","year":"2016"},{"key":"10.1016\/j.fsi.2018.03.028_bib12","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.dci.2016.03.024","article-title":"Oral vaccination of fish: lessons from humans and veterinary species","volume":"64","author":"Embregts","year":"2016","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib13","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.3389\/fimmu.2017.01340","article-title":"Intramuscular DNA vaccination of juvenile carp against spring viremia of carp virus induces full protection and establishes a virus-specific B and T Cell response","volume":"8","author":"Embregts","year":"2017","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib14","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1186\/1756-0500-4-210","article-title":"Antibody recognition of the glycoprotein g of viral haemorrhagic septicemia virus (VHSV) purified in large amounts from insect larvae","volume":"4","author":"Encinas","year":"2011","journal-title":"BMC Res. Notes"},{"key":"10.1016\/j.fsi.2018.03.028_bib15","first-page":"125","article-title":"Isolation of the viral causative agent from the acute form of infectious dropsy of carp","volume":"41","author":"Fijan","year":"1971","journal-title":"Vet. Arh."},{"key":"10.1016\/j.fsi.2018.03.028_bib16","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/978-1-61779-439-1_2","article-title":"The use of real-time quantitative PCR for the analysis of cytokine mRNA levels","volume":"820","author":"Forlenza","year":"2012","journal-title":"Meth. Mol. Biol."},{"key":"10.1016\/j.fsi.2018.03.028_bib17","doi-asserted-by":"crossref","first-page":"3178","DOI":"10.1016\/j.molimm.2008.02.025","article-title":"Differential contribution of neutrophilic granulocytes and macrophages to nitrosative stress in a host-parasite animal model","volume":"45","author":"Forlenza","year":"2008","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib18","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.fsi.2007.12.013","article-title":"Transcriptional analysis of the common carp (Cyprinus carpio L.) immune response to the fish louse Argulus japonicus Thiele (Crustacea: branchiura)","volume":"25","author":"Forlenza","year":"2008","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib19","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/0264-410X(93)90379-C","article-title":"Oral vaccination of racoons (Procyon Iotor) with baculovirus-expressed rabies virus glycoprotein","volume":"11","author":"Fu","year":"1993","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2018.03.028_bib20","doi-asserted-by":"crossref","first-page":"5716","DOI":"10.1128\/IAI.69.9.5716-5725.2001","article-title":"Cholera toxin B subunit as a Carrier molecule promotes antigen presentation and increases CD40 and CD86 expression on antigen-presenting cells","volume":"69","author":"George-Chandy","year":"2001","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fsi.2018.03.028_bib21","first-page":"334","article-title":"Characterization of spring viremia of carp virus glycoprotein expressed by recombinant baculovirus","volume":"32","author":"Huang","year":"2012","journal-title":"Pak. Vet. J."},{"key":"10.1016\/j.fsi.2018.03.028_bib22","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/0044-8486(95)91961-T","article-title":"Genetic variability of Polish and Hungarian carp lines","volume":"129","author":"Irnazarow","year":"1995","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2018.03.028_bib23","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/0145-305X(94)90251-8","article-title":"Characterisation of immunoglobulin-binding leucocytes in carp (Cyprinus carpio L.)","volume":"18","author":"Koumans-van Diepen","year":"1994","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib24","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0966-842X(97)01145-1","article-title":"The role of CpG dinucleotides in DNA vaccines","volume":"6","author":"Krieg","year":"1998","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.fsi.2018.03.028_bib25","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.fsi.2004.10.009","article-title":"Time course study of in situ expression of antigens following DNA-vaccination against VHS in rainbow trout (Oncorhynchus mykiss Walbaum) fry","volume":"19","author":"Lorenzen","year":"2005","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib26","doi-asserted-by":"crossref","first-page":"201","DOI":"10.20506\/rst.24.1.1565","article-title":"DNA vaccines for aquacultured fish","volume":"24","author":"Lorenzen","year":"2005","journal-title":"Rev. Sci. Tech"},{"key":"10.1016\/j.fsi.2018.03.028_bib27","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1016\/j.vaccine.2010.11.073","article-title":"The introduction of multi-copy CpG motifs into an antiviral DNA vaccine strongly up-regulates its immunogenicity in fish","volume":"29","author":"Martinez-Alonso","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2018.03.028_bib28","doi-asserted-by":"crossref","first-page":"427","DOI":"10.3389\/fimmu.2015.00427","article-title":"A review of the immunological mechanisms following mucosal vaccination of finfish","volume":"6","author":"Munang\u2019andu","year":"2015","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib29","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2015.00542","article-title":"An overview of challenges limiting the design of protective mucosal vaccines for finfish","volume":"6","author":"Munang\u2019andu","year":"2015","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib30","doi-asserted-by":"crossref","first-page":"519","DOI":"10.3389\/fimmu.2015.00519","article-title":"Oral vaccination of fish- antigen preparations, uptake and immune induction","volume":"6","author":"Mutoloki","year":"2015","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib31","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1006\/fsim.1997.0125","article-title":"Production of a monoclonal antibody for carp (Cyprinus carpio L.) phagocytic cells and separation of the cells","volume":"8","author":"Nakayasu","year":"1998","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib32","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1128\/CVI.00538-10","article-title":"Characterization of a mutant Escherichia coli heat-labile toxin, LT(R192G\/L211A), as a safe and effective oral adjuvant","volume":"18","author":"Norton","year":"2011","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib33","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.dci.2016.03.003","article-title":"Long-lived effects of administering \u03b2-glucans: indications for trained immunity in fish","volume":"64","author":"Petit","year":"2016","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib34","series-title":"Immunogenic and Protective Properties of Rabies Virus Glycoprotein Expressed by Baculovirus Vectors","author":"Prehaud","year":"1989"},{"key":"10.1016\/j.fsi.2018.03.028_bib35","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Epidemiol."},{"key":"10.1016\/j.fsi.2018.03.028_bib36","doi-asserted-by":"crossref","first-page":"50","DOI":"10.7774\/cevr.2012.1.1.50","article-title":"Mucosal vaccine adjuvants update","volume":"1","author":"Rhee","year":"2012","journal-title":"Clin. Exp. Vaccine Res."},{"key":"10.1016\/j.fsi.2018.03.028_bib37","series-title":"Systemic and\/or Mucosal Immune Responses after Immunization with Soluble or Particulate Antigen","first-page":"179","article-title":"Immunological importance of the second gut segment of carp. 111","author":"Rombout","year":"1989"},{"key":"10.1016\/j.fsi.2018.03.028_bib38","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/S0145-305X(97)00048-7","article-title":"Indications for a distinct putative T cell population in mucosal tissue of carp (Cyprinus carpio L.)","volume":"22","author":"Rombout","year":"1998","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib39","first-page":"165","article-title":"Separation of lymphocyte subpopulations in carp Cyprinus carpio L. by monoclonal antibodies: immunohistochemical studies","volume":"48","author":"Secombes","year":"1983","journal-title":"Immunology"},{"key":"10.1016\/j.fsi.2018.03.028_bib40","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1111\/apm.12351","article-title":"Mucosal vaccines: a paradigm shift in the development of mucosal adjuvants and delivery vehicles","volume":"123","author":"Srivastava","year":"2015","journal-title":"Apmis"},{"key":"10.1016\/j.fsi.2018.03.028_bib41","doi-asserted-by":"crossref","first-page":"1740","DOI":"10.1016\/j.fsi.2013.02.029","article-title":"Adjuvants and immunostimulants in fish vaccines: current knowledge and future perspectives","volume":"35","author":"Tafalla","year":"2013","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib42","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1007\/s00705-007-0971-8","article-title":"Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp (Cyprinus carpio) in China","volume":"152","author":"Teng","year":"2007","journal-title":"Arch. Virol."},{"key":"10.1016\/j.fsi.2018.03.028_bib43","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.dci.2007.05.010","article-title":"Cell-mediated immune responses in rainbow trout after DNA immunization against the viral hemorrhagic septicemia virus","volume":"32","author":"Utke","year":"2008","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib44","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1006\/fsim.1996.0069","article-title":"A common carp (Cyprinus carpio L.) leucocyte cell line shares morphological and functional characteristics with macrophages","volume":"7","author":"Weyts","year":"1997","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2018.03.028_bib45","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/S0165-2427(97)00124-4","article-title":"Induction of oral tolerance in carp (Cyprinus carpio L.) after feeding protein antigens","volume":"60","author":"Joosten","year":"1997","journal-title":"Vet. Immunol. Immunopathol."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464818301451?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464818301451?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T05:10:16Z","timestamp":1760937016000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464818301451"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2]]},"references-count":45,"alternative-id":["S1050464818301451"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2018.03.028","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2019,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Vaccination of carp against SVCV with an oral DNA vaccine or an insect cells-based subunit vaccine","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2018.03.028","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}