{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T01:34:46Z","timestamp":1789004086720,"version":"build-2803163510"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,9,1]],"date-time":"2018-09-01T00:00:00Z","timestamp":1535760000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31672646"],"award-info":[{"award-number":["31672646"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"crossref","award":["201762016"],"award-info":[{"award-number":["201762016"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2018,9]]},"DOI":"10.1016\/j.fsi.2018.05.058","type":"journal-article","created":{"date-parts":[[2018,5,31]],"date-time":"2018-05-31T02:19:29Z","timestamp":1527733169000},"page":"22-30","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":25,"special_numbering":"C","title":["Kunitz-type serine protease inhibitor is a novel participator in anti-bacterial and anti-inflammatory responses in Japanese flounder ( Paralichthys olivaceus )"],"prefix":"10.1016","volume":"80","author":[{"given":"Xin","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxiang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujue","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Si","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuangang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhigang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanqi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyang","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xubo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2018.05.058_bib1","first-page":"68","article-title":"Plant protease inhibitors: a defense strategy in plants","volume":"2","author":"Habib","year":"2007","journal-title":"Biotechnol. Mol. Biol. Rev."},{"key":"10.1016\/j.fsi.2018.05.058_bib2","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.phytochem.2013.09.010","article-title":"Phytochemistry purification and characterization of a stable Kunitz trypsin inhibitor from Trigonella foenum-graecum (fenugreek) seeds","volume":"96","author":"Oddepally","year":"2013","journal-title":"Phytochemisty"},{"key":"10.1016\/j.fsi.2018.05.058_bib3","doi-asserted-by":"crossref","first-page":"1943","DOI":"10.1016\/j.molimm.2010.04.020","article-title":"Serine proteases of the human immune system in health and disease","volume":"47","author":"Heutinck","year":"2010","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2018.05.058_bib4","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0145-305X(99)00012-9","article-title":"Serine proteinase inhibitors in arthropod","volume":"23","author":"Kanost","year":"1999","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2018.05.058_bib5","doi-asserted-by":"crossref","first-page":"10363","DOI":"10.1021\/bi00107a001","article-title":"The coagulation cascade: initiation, maintenance, and regulation","volume":"30","author":"Davie","year":"1991","journal-title":"Biochemistry"},{"key":"10.1016\/j.fsi.2018.05.058_bib6","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1042\/bst0300116","article-title":"Novel roles of protease inhibitors in infection and inflammation","volume":"30","author":"Hiemstra","year":"2002","journal-title":"Biochem. Soc. Trans."},{"key":"10.1016\/j.fsi.2018.05.058_bib7","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1146\/annurev.bi.50.070181.002245","article-title":"The proteolytic activation systems of complement","volume":"50","author":"Reid","year":"1981","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.fsi.2018.05.058_bib8","doi-asserted-by":"crossref","first-page":"14923","DOI":"10.1074\/jbc.M500531200","article-title":"Manduca sexta serpin-4 and serpin-5 inhibit the proph-enol oxidase activation pathway: cDNA cloning, protein expression, and characterization","volume":"280","author":"Tong","year":"2005","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fsi.2018.05.058_bib9","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1007\/s00011-010-0205-5","article-title":"Anti- inflammatory actions of serine protease inhibitors containing the Kunitz domain","volume":"59","author":"Shigetomi","year":"2010","journal-title":"Inflamm. Res."},{"key":"10.1016\/j.fsi.2018.05.058_bib10","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1146\/annurev.bi.49.070180.003113","article-title":"Protein inhibitors of proteinases","volume":"49","author":"Laskowski","year":"1980","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.fsi.2018.05.058_bib11","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0145-305X(99)00012-9","article-title":"Serine proteinase inhibitors in arthropod immunity","volume":"23","author":"Kanost","year":"1999","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2018.05.058_bib12","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1111\/j.1432-1033.1992.tb16654.x","article-title":"Natural protein proteinase inhibitors and their interaction with proteinase","volume":"204","author":"Bode","year":"1992","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.fsi.2018.05.058_bib13","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.toxicon.2017.10.019","article-title":"Purification and characterization of an anti-hemorrhagic protein from Naja naja (Indian cobra) venom","volume":"140","author":"Suvilesh","year":"2017","journal-title":"Toxicon Official Journal of the International Society on Toxinology"},{"key":"10.1016\/j.fsi.2018.05.058_bib14","doi-asserted-by":"crossref","first-page":"12202","DOI":"10.1074\/jbc.272.18.12202","article-title":"Identification and cloning of human placental bikunin, a novel serine protease inhibitor containing two Kunitz domains","volume":"272","author":"Marlor","year":"1997","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fsi.2018.05.058_bib15","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1016\/j.biochi.2010.03.021","article-title":"A novel sub-classification for Kunitz proteinase inhibitors from leguminous seeds","volume":"92","author":"Oliva","year":"2010","journal-title":"Biochimie"},{"key":"10.1016\/j.fsi.2018.05.058_bib16","first-page":"259","article-title":"Identification and characterization of protease inhibition effect of Kunitz-type serine protease inhibitor 1 (NaKuI1) from Necator americanus","volume":"35","author":"Sui","year":"2017","journal-title":"Chin. J. Parasitol. Parasit. Dis."},{"key":"10.1016\/j.fsi.2018.05.058_bib17","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.dci.2012.10.005","article-title":"Structure and function of invertebrate Kunitz serine protease inhibitors","volume":"39","author":"Ranasinghea","year":"2013","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2018.05.058_bib18","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1515\/BC.2005.066","article-title":"Kunitz- type Bauhinia bauhinioides inhibitors devoid of disulfide bridges: isolation of the cDNA, heterologous expression and structural studies","volume":"386","author":"Araujo","year":"2005","journal-title":"Biol. Chem."},{"key":"10.1016\/j.fsi.2018.05.058_bib19","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1016\/j.fsi.2013.06.023","article-title":"Molecular and functional characterizations of a kunitz-type serine protease inhibitor fckuspi of the shrimp fenneropenaeus chinensis","volume":"35","author":"Kong","year":"2013","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2018.05.058_bib20","first-page":"2374","article-title":"Cloning and expression analysis of KuSPI gene of Hypriopsis cumingi","volume":"36","author":"Wang","year":"2017","journal-title":"Jiyinzuxue Yu Yingyong Shengwuxue (Genomics and Applied Biology)"},{"key":"10.1016\/j.fsi.2018.05.058_bib21","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1016\/j.fsi.2012.09.015","article-title":"A four-domain kunitz-type proteinase inhibitor from solen grandis is implicated in immune response","volume":"3","author":"Wei","year":"2012","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2018.05.058_bib22","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.fsi.2015.07.004","article-title":"A homolog of kunitz-type serine protease inhibitor from rock bream, Oplegnathus fasciatus: molecular insights and transcriptional modulation in response to microbial and PAMP stimulation, and tissue injury","volume":"46","author":"Bathige","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2018.05.058_bib23","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1179\/016164110X12645013515133","article-title":"Urinary trypsin inhibitor suppresses excessive superoxide anion radical generation in blood, oxidative stress, early inflammation, and endothelial injury in forebrain ischemia\/reperfusion rats","volume":"32","author":"Koga","year":"2010","journal-title":"Neurol. Res."},{"key":"10.1016\/j.fsi.2018.05.058_bib24","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1134\/S106816201506014X","article-title":"Anti-inflammatory activity of a polypeptide from the Heteractis crispa, sea anemone","volume":"41","author":"Sintsova","year":"2015","journal-title":"Russ. J. Bioorg. Chem."},{"key":"10.1016\/j.fsi.2018.05.058_bib25","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1111\/j.1365-2761.2006.00750.x","article-title":"Characterization of Edwardsiella tarda strains isolated from turbot, Psetta maxima (L.)","volume":"29","author":"Castro","year":"2006","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2018.05.058_bib26","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1007\/s12038-007-0143-8","article-title":"Edwardsiellosis in fish: a brief review","volume":"32","author":"Mohanty","year":"2007","journal-title":"J. Biosci."},{"key":"10.1016\/j.fsi.2018.05.058_bib27","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1038\/ng.3732","article-title":"The genome and transcriptome of Japanese flounder provide insights into flatfish asymmetry","volume":"49","author":"Shao","year":"2017","journal-title":"Nat. Genet."},{"key":"10.1016\/j.fsi.2018.05.058_bib28","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.biochi.2011.08.003","article-title":"Identification and characterisation of Kunitz-type plasma kallikrein inhibitors unique to Oxyuranus sp. snake venoms","volume":"94","author":"Earl","year":"2012","journal-title":"Biochimie"},{"key":"10.1016\/j.fsi.2018.05.058_bib29","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.cbpb.2008.03.010","article-title":"Kunitz-type protease inhibitors from acrorhagi of three species of sea anemones","volume":"150","author":"Minagawa","year":"2008","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"10.1016\/j.fsi.2018.05.058_bib30","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.vetpar.2009.09.030","article-title":"Biochemical characterization of a Kunitz type inhibitor similar to dendrotoxins produced by Rhipicephalus (Boophilus) microplus (Acari: ixodidae) hemocytes","volume":"167","author":"Lima","year":"2010","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.fsi.2018.05.058_bib31","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1749-0774.2008.00062.x","article-title":"Tissue injury alters the site of expression of hepatocyte growth factor activator inhibitor type 1 in bronchial epithelial cells","volume":"22","author":"Tanaka","year":"2009","journal-title":"Hum. Cell"},{"key":"10.1016\/j.fsi.2018.05.058_bib32","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.toxicon.2011.10.005","article-title":"Functional characterization of Kunitz-type protease inhibitor Pr-mulgins identified from New Guinean Pseudechis australis","volume":"59","author":"Inagaki","year":"2012","journal-title":"Toxicon"},{"key":"10.1016\/j.fsi.2018.05.058_bib33","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.vetpar.2008.03.031","article-title":"RBmTI-6, a Kunitz- BPTI domain protease inhibitor from the tick Boophilus microplus, its cloning, expression and biochemical characterization","volume":"155","author":"Sasaki","year":"2008","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.fsi.2018.05.058_bib34","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1016\/j.ibmb.2010.08.007","article-title":"Characterization of Kunitz-type protease inhibitor purified from hemolymph of Galleria mellonella larvae","volume":"40","author":"Lee","year":"2010","journal-title":"Insect Biochem. Mol. Biol."},{"key":"10.1016\/j.fsi.2018.05.058_bib35","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1006\/jmbi.2000.3935","article-title":"Sub-stitutions at the P(1) position in BPTI strongly affect the association energy with serine proteinases","volume":"301","author":"Grzesiak","year":"2000","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.fsi.2018.05.058_bib36","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/0167-5699(89)90085-6","article-title":"The cytokine network","volume":"10","author":"Balkwill","year":"1989","journal-title":"Immunol. Today"},{"key":"10.1016\/j.fsi.2018.05.058_bib37","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1111\/j.1469-7793.1999.287ad.x","article-title":"Pro- and anti-inflammatory cytokine balance in strenuous exercise in humans","volume":"515","author":"Ostrowski","year":"1999","journal-title":"J. Physiol."},{"key":"10.1016\/j.fsi.2018.05.058_bib38","article-title":"Urinary trypsin inhibitor reduces LPS-induced hypotension by suppressing tumor necrosis factor-alpha production through inhibition of egr-1 expression","volume":"288","author":"Molorerdene","year":"2005","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"10.1016\/j.fsi.2018.05.058_bib39","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1097\/01.shk.0000160940.16008.a5","article-title":"Dietary supplementation of soybean kunitz trypsin inhibitor reduces lipopolysaccharide- induced lethality in mouse model","volume":"23","author":"Kobayashi","year":"2005","journal-title":"Shock"},{"key":"10.1016\/j.fsi.2018.05.058_bib40","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1126\/science.1072391","article-title":"Activation of Drosophila Toll during fungal infection by a blood serine protease","volume":"297","author":"Ligoxygakis","year":"2002","journal-title":"Science (Wash. D C)"},{"key":"10.1016\/j.fsi.2018.05.058_bib41","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1111\/cmi.12106","article-title":"Eprs, an autotransporter protein of pseudomonas aeruginosa, possessing serine protease activity induces inflammatory responses through protease-activated receptors","volume":"15","author":"Kida","year":"2013","journal-title":"Cell Microbiol."},{"key":"10.1016\/j.fsi.2018.05.058_bib42","doi-asserted-by":"crossref","DOI":"10.3390\/biology5020023","article-title":"The function of fish cytokines","volume":"5","author":"Zou","year":"2016","journal-title":"Biology"},{"key":"10.1016\/j.fsi.2018.05.058_bib43","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.cbpb.2013.10.002","article-title":"A bumblebee (Bombus ignitus) venom serine protease inhibitor that acts as a microbial serine protease inhibitor","volume":"167","author":"Wan","year":"2014","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"10.1016\/j.fsi.2018.05.058_bib44","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1038\/nrmicro1098","article-title":"Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria","volume":"3","author":"Brogden","year":"2005","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.fsi.2018.05.058_bib45","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1128\/CMR.00056-05","article-title":"Peptide antimicrobial agents","volume":"19","author":"Jenssen","year":"2006","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.fsi.2018.05.058_bib46","first-page":"217","article-title":"Pathogenic mechanisms induced by microbial proteases in microbial infections","volume":"377","author":"Maeda","year":"1996","journal-title":"Biol. Chem. Hoppe-Seyler"},{"key":"10.1016\/j.fsi.2018.05.058_bib47","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1128\/IAI.67.5.2184-2192.1999","article-title":"Cytoskeletal effects induced by pet, the serine protease enterotoxin of enteroaggregative Escherichia coli","volume":"67","author":"Navarrogarc\u00eda","year":"1999","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fsi.2018.05.058_bib48","doi-asserted-by":"crossref","first-page":"257","DOI":"10.3109\/15569548209019477","article-title":"Toxins of Vibrio parahaemolyticus and Aeromonas hydrophila","volume":"1","author":"Ljungh","year":"2008","journal-title":"J. Toxicol. Toxin Rev."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464818303280?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464818303280?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T22:08:23Z","timestamp":1604354903000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464818303280"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9]]},"references-count":48,"alternative-id":["S1050464818303280"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2018.05.058","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2018,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Kunitz-type serine protease inhibitor is a novel participator in anti-bacterial and anti-inflammatory responses in Japanese flounder (Paralichthys olivaceus)","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2018.05.058","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}