{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T16:52:18Z","timestamp":1780505538832,"version":"3.54.1"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"European Commission under the Seventh Framework Programme for Research and Technological Development (FP7) of the European Union","award":["311993"],"award-info":[{"award-number":["311993"]}]},{"name":"Horizon H2020 research and innovation programme","award":["H2020-634429"],"award-info":[{"award-number":["H2020-634429"]}]},{"name":"CONICYT"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2019,2]]},"DOI":"10.1016\/j.fsi.2018.06.056","type":"journal-article","created":{"date-parts":[[2018,6,30]],"date-time":"2018-06-30T19:00:42Z","timestamp":1530385242000},"page":"99-105","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Time-course study of the protection induced by an interferon-inducible DNA vaccine against viral haemorrhagic septicaemia in rainbow trout"],"prefix":"10.1016","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8553-5861","authenticated-orcid":false,"given":"Dagoberto","family":"Sep\u00falveda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Lorenzen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesper Skou","family":"Rasmussen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Einer-Jensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2795-2653","authenticated-orcid":false,"given":"Bertrand","family":"Collet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8781-176X","authenticated-orcid":false,"given":"C.J.","family":"Secombes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels","family":"Lorenzen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2018.06.056_bib1","first-page":"563","article-title":"Family rhabdoviridae","author":"Walker","year":"2000","journal-title":"Virus taxonomy"},{"issue":"9","key":"10.1016\/j.fsi.2018.06.056_bib2","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1111\/j.1365-2761.2005.00654.x","article-title":"Viral haemorrhagic septicaemia virus in marine fish and its implications for fish farming\u2013a review","volume":"28","author":"Skall","year":"2005","journal-title":"J. Fish. Dis."},{"issue":"5\u20136","key":"10.1016\/j.fsi.2018.06.056_bib3","first-page":"379","article-title":"Eighteen years of vaccination against viral haemorrhagic septicaemia in France","volume":"26","author":"De Kinkelin","year":"1995","journal-title":"Vet. Res. (Paris)"},{"key":"10.1016\/j.fsi.2018.06.056_bib4","first-page":"201","article-title":"Immunization with viral antigens: viral haemorrhagic septicaemia","volume":"90","author":"Lorenzen","year":"1996","journal-title":"Dev. Biol. Stand."},{"issue":"6","key":"10.1016\/j.fsi.2018.06.056_bib5","doi-asserted-by":"crossref","first-page":"1759","DOI":"10.1016\/j.fsi.2013.05.029","article-title":"Status and future perspectives of vaccines for industrialised fin-fish farming","volume":"35","author":"Brudeseth","year":"2013","journal-title":"Fish Shellfish Immunol."},{"issue":"3","key":"10.1016\/j.fsi.2018.06.056_bib6","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1080\/23308249.2016.1261277","article-title":"Advances in aquaculture vaccines against fish pathogens: global status and current trends","volume":"25","author":"Dadar","year":"2017","journal-title":"Reviews in Fisheries Science & Aquaculture"},{"issue":"1","key":"10.1016\/j.fsi.2018.06.056_bib7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s13337-013-0186-4","article-title":"Viral vaccines for farmed finfish","volume":"25","author":"Dhar","year":"2014","journal-title":"Virusdisease"},{"issue":"4","key":"10.1016\/j.fsi.2018.06.056_bib8","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1006\/fsim.1997.0133","article-title":"Development of DNA vaccines for fish: vector design, intramuscular injection and antigen expression using viral haemorrhagic septicaemia virus genes as model","volume":"8","author":"Heppell","year":"1998","journal-title":"Fish Shellfish Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2018.06.056_bib9","first-page":"201","article-title":"DNA vaccines for aquacultured fish, revue scientifique et technique-office","volume":"24","author":"Lorenzen","year":"2005","journal-title":"International Des Epizooties"},{"issue":"1","key":"10.1016\/j.fsi.2018.06.056_bib10","first-page":"1","article-title":"Fish rhabdovirus models for understanding host response to DNA vaccines","volume":"2","author":"Kurath","year":"2007","journal-title":"CAB reviews"},{"issue":"2","key":"10.1016\/j.fsi.2018.06.056_bib11","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1006\/viro.1998.9322","article-title":"Combined DNA immunization with the glycoprotein gene of viral hemorrhagic septicemia virus and infectious hematopoietic necrosis virus induces double-specific protective immunity and nonspecific response in rainbow trout","volume":"249","author":"Boudinot","year":"1998","journal-title":"Virology"},{"issue":"13","key":"10.1016\/j.fsi.2018.06.056_bib12","doi-asserted-by":"crossref","first-page":"2089","DOI":"10.1016\/j.molimm.2005.12.005","article-title":"Comprehensive gene expression profiling following DNA vaccination of rainbow trout against infectious hematopoietic necrosis virus","volume":"43","author":"Purcell","year":"2006","journal-title":"Mol. Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2018.06.056_bib13","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.fsi.2004.06.005","article-title":"Kinetics of Mx expression in rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar L.) parr in response to VHS\u2013DNA vaccination","volume":"18","author":"Acosta","year":"2005","journal-title":"Fish Shellfish Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2018.06.056_bib14","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/S1050-4648(02)00137-7","article-title":"DNA vaccination against viral haemorrhagic septicaemia (VHS) in rainbow trout: size, dose, route of injection and duration of protection\u2014early protection correlates with Mx expression","volume":"15","author":"McLauchlan","year":"2003","journal-title":"Fish Shellfish Immunol."},{"issue":"15","key":"10.1016\/j.fsi.2018.06.056_bib15","doi-asserted-by":"crossref","first-page":"7048","DOI":"10.1128\/JVI.74.15.7048-7054.2000","article-title":"DNA vaccines encoding viral glycoproteins induce nonspecific immunity and Mx protein synthesis in fish","volume":"74","author":"Kim","year":"2000","journal-title":"J. Virol."},{"issue":"3","key":"10.1016\/j.fsi.2018.06.056_bib16","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1577\/1548-8667(2000)012<0167:FVORTA>2.0.CO;2","article-title":"DNA vaccination of rainbow trout against viral hemorrhagic septicemia virus: a dose\u2013response and time\u2013course study","volume":"12","author":"Lorenzen","year":"2000","journal-title":"J. Aquat. Anim. Health"},{"issue":"3","key":"10.1016\/j.fsi.2018.06.056_bib17","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.dci.2007.05.010","article-title":"Cell-mediated immune responses in rainbow trout after DNA immunization against the viral hemorrhagic septicemia virus","volume":"32","author":"Utke","year":"2008","journal-title":"Dev. Comp. Immunol."},{"issue":"3","key":"10.1016\/j.fsi.2018.06.056_bib18","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.vaccine.2005.07.068","article-title":"Protective immunity and lack of histopathological damage two years after DNA vaccination against infectious hematopoietic necrosis virus in trout","volume":"24","author":"Kurath","year":"2006","journal-title":"Vaccine"},{"issue":"4","key":"10.1016\/j.fsi.2018.06.056_bib19","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1016\/j.fsi.2007.03.015","article-title":"Detection of supercoiled plasmid DNA and luciferase expression in Atlantic salmon (Salmo salar L.) 535 days after injection","volume":"23","author":"Tonheim","year":"2007","journal-title":"Fish Shellfish Immunol."},{"issue":"8","key":"10.1016\/j.fsi.2018.06.056_bib20","first-page":"635","article-title":"The road to licensure of a DNA vaccine","volume":"8","author":"Salonius","year":"2007","journal-title":"Curr. Opin. Invest. Drugs"},{"issue":"4","key":"10.1016\/j.fsi.2018.06.056_bib21","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1038\/mt.2008.5","article-title":"Physiological promoters reduce the genotoxic risk of integrating gene vectors","volume":"16","author":"Zychlinski","year":"2008","journal-title":"Mol. Ther."},{"issue":"4","key":"10.1016\/j.fsi.2018.06.056_bib22","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.biotechadv.2009.02.003","article-title":"Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream production","volume":"27","author":"Williams","year":"2009","journal-title":"Biotechnol. Adv."},{"issue":"13","key":"10.1016\/j.fsi.2018.06.056_bib23","doi-asserted-by":"crossref","first-page":"1938","DOI":"10.1016\/j.vaccine.2009.01.103","article-title":"The immunogenicity of viral haemorragic septicaemia rhabdovirus (VHSV) DNA vaccines can depend on plasmid regulatory sequences","volume":"27","author":"Chico","year":"2009","journal-title":"Vaccine"},{"issue":"15","key":"10.1016\/j.fsi.2018.06.056_bib24","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1016\/S0264-410X(02)00749-1","article-title":"A fish specific expression vector containing the interferon regulatory factor 1A (IRF1A) promoter for genetic immunization of fish","volume":"21","author":"Alonso","year":"2003","journal-title":"Vaccine"},{"issue":"1","key":"10.1016\/j.fsi.2018.06.056_bib25","doi-asserted-by":"crossref","first-page":"62","DOI":"10.2174\/1872215611307010009","article-title":"Licensed DNA vaccines against infectious hematopoietic necrosis virus (IHNV)","volume":"7","author":"Alonso","year":"2013","journal-title":"Recent Patents on DNA & Gene Sequences"},{"issue":"7","key":"10.1016\/j.fsi.2018.06.056_bib26","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1016\/j.dci.2003.12.005","article-title":"An Mx1 promoter\u2013reporter system to study interferon pathways in rainbow trout","volume":"28","author":"Collet","year":"2004","journal-title":"Dev. Comp. Immunol."},{"issue":"6","key":"10.1016\/j.fsi.2018.06.056_bib27","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1046\/j.1432-1327.2001.02021.x","article-title":"The rainbow trout (Oncorhynchus mykiss) Mx1 promoter","volume":"268","author":"Collet","year":"2001","journal-title":"Eur. J. Biochem."},{"issue":"2","key":"10.1016\/j.fsi.2018.06.056_bib28","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.jbiotec.2012.08.005","article-title":"Replacement of the human cytomegalovirus promoter with fish enhancer and core elements to control the expression of the G gene of viral haemorrhagic septicemia virus (VHSV)","volume":"164","author":"Martinez-Lopez","year":"2013","journal-title":"J. Biotechnol."},{"issue":"1","key":"10.1016\/j.fsi.2018.06.056_bib29","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.fsi.2004.10.009","article-title":"\u00d8. Evensen, Time course study of in situ expression of antigens following DNA-vaccination against VHS in rainbow trout (Oncorhynchus mykiss Walbaum) fry","volume":"19","author":"Lorenzen","year":"2005","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2018.06.056_bib30","series-title":"Some properties of the epithelioma papulosum cyprini (EPC) cell line from carp Cyprinus carpio, Annales de l'Institut Pasteur\/Virologie","first-page":"207","author":"Fijan","year":"1983"},{"issue":"3713","key":"10.1016\/j.fsi.2018.06.056_bib31","doi-asserted-by":"crossref","first-page":"1004","DOI":"10.1126\/science.151.3713.1004","article-title":"Lymphocystis virus: isolation and propagation in centrarchid fish cell lines","volume":"151","author":"Wolf","year":"1966","journal-title":"Science"},{"issue":"4","key":"10.1016\/j.fsi.2018.06.056_bib32","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1093\/jnci\/56.4.843","article-title":"Epithelial cell cultures from normal and cancerous human tissues","volume":"56","author":"Owens","year":"1976","journal-title":"J. Natl. Cancer Inst. (Bethesda)"},{"issue":"3","key":"10.1016\/j.fsi.2018.06.056_bib33","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Epidemiol."},{"issue":"2","key":"10.1016\/j.fsi.2018.06.056_bib34","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0145-305X(01)00059-3","article-title":"Immunity induced shortly after DNA vaccination of rainbow trout against rhabdoviruses protects against heterologous virus but not against bacterial pathogens","volume":"26","author":"Lorenzen","year":"2002","journal-title":"Dev. Comp. Immunol."},{"issue":"4","key":"10.1016\/j.fsi.2018.06.056_bib35","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1006\/fsim.1997.0134","article-title":"Protective immunity to VHS in rainbow trout (Oncorhynchus mykiss, Walbaum) following DNA vaccination","volume":"8","author":"Lorenzen","year":"1998","journal-title":"Fish Shellfish Immunol."},{"issue":"25","key":"10.1016\/j.fsi.2018.06.056_bib36","doi-asserted-by":"crossref","first-page":"2817","DOI":"10.1016\/S0264-410X(00)00078-5","article-title":"Nanogram quantities of a DNA vaccine protect rainbow trout fry against heterologous strains of infectious hematopoietic necrosis virus","volume":"18","author":"Corbeil","year":"2000","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2018.06.056_bib37","series-title":"R: a Language and Environment for Statistical Computing","first-page":"2017","author":"Team","year":"2014"},{"key":"10.1016\/j.fsi.2018.06.056_bib38","series-title":"R-Studio: Integrated Development for R","author":"Team","year":"2015"},{"key":"10.1016\/j.fsi.2018.06.056_bib39","doi-asserted-by":"crossref","first-page":"99","DOI":"10.2307\/3001913","article-title":"Comparing individual means in the analysis of variance","author":"Tukey","year":"1949","journal-title":"Biometrics"},{"issue":"24","key":"10.1016\/j.fsi.2018.06.056_bib40","doi-asserted-by":"crossref","first-page":"4904","DOI":"10.1016\/j.jmb.2013.09.033","article-title":"The antiviral innate immune response in fish: evolution and conservation of the IFN system","volume":"425","author":"Langevin","year":"2013","journal-title":"J. Mol. Biol."},{"issue":"1","key":"10.1016\/j.fsi.2018.06.056_bib41","doi-asserted-by":"crossref","first-page":"2109","DOI":"10.1186\/s40064-016-3729-5","article-title":"Molecular cloning, polymorphism, and functional activity of the bovine and water buffalo Mx2 gene promoter region","volume":"5","author":"Babiker","year":"2016","journal-title":"SpringerPlus"},{"key":"10.1016\/j.fsi.2018.06.056_bib42","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.gene.2003.10.006","article-title":"Genomic structure, organisation, and promoter analysis of the bovine (Bos taurus) Mx1 gene","volume":"326","author":"G\u00e9rardin","year":"2004","journal-title":"Gene"},{"issue":"7","key":"10.1016\/j.fsi.2018.06.056_bib43","doi-asserted-by":"crossref","first-page":"855","DOI":"10.5713\/ajas.2010.90472","article-title":"Expression analysis of the Mx gene and its genome structure in chickens","volume":"23","author":"Yin","year":"2010","journal-title":"Asian-australas. J. Anim. Sci."},{"key":"10.1016\/j.fsi.2018.06.056_bib44","doi-asserted-by":"crossref","first-page":"79","DOI":"10.21775\/cimb.022.079","article-title":"DNA vaccines: regulatory considerations and safety aspects","volume":"5","author":"Myhr","year":"2017","journal-title":"Curr. Issues Mol. Biol."},{"issue":"2","key":"10.1016\/j.fsi.2018.06.056_bib45","first-page":"114","article-title":"Genetic immunization of rainbow trout (Oncorhynchus mykiss) against infectious hematopoietic necrosis virus","volume":"5","author":"Anderson","year":"1996","journal-title":"Mol. Mar. Biol. Biotechnol."},{"issue":"21","key":"10.1016\/j.fsi.2018.06.056_bib46","doi-asserted-by":"crossref","first-page":"2442","DOI":"10.1016\/j.vaccine.2015.03.093","article-title":"Adjuvant activity of fish type I interferon shown in a virus DNA vaccination model","volume":"33","author":"Chang","year":"2015","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2018.06.056_bib47","first-page":"651","article-title":"Using plasmids as DNA vaccines for infectious diseases, plasmids: biology and impact in biotechnology and discovery","author":"Tregoning","year":"2015","journal-title":"American Society of Microbiology"},{"key":"10.1016\/j.fsi.2018.06.056_bib48","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.dci.2017.02.005","article-title":"The role of type I interferons in innate and adaptive immunity against viruses in Atlantic salmon","volume":"80","author":"Robertsen","year":"2018","journal-title":"Dev. Comp. Immunol."}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464818303978?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464818303978?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T05:09:32Z","timestamp":1760936972000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464818303978"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2]]},"references-count":48,"alternative-id":["S1050464818303978"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2018.06.056","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2019,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Time-course study of the protection induced by an interferon-inducible DNA vaccine against viral haemorrhagic septicaemia in rainbow trout","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2018.06.056","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}