{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T17:58:26Z","timestamp":1787421506054,"version":"build-2736575974"},"reference-count":55,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries, Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Marine Biotechnology Program","award":["20180430"],"award-info":[{"award-number":["20180430"]}]},{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries, Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2019,1]]},"DOI":"10.1016\/j.fsi.2018.10.010","type":"journal-article","created":{"date-parts":[[2018,10,6]],"date-time":"2018-10-06T15:52:51Z","timestamp":1538841171000},"page":"223-232","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Identification and characterization of a carboxypeptidase N1 from red lip mullet (Liza haematocheila); revealing its immune relevance"],"prefix":"10.1016","volume":"84","author":[{"given":"N.C.N.","family":"Perera","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.I.","family":"Godahewa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumi","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myoung-Jin","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo-Hye","family":"Nam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8851-6121","authenticated-orcid":false,"given":"Jehee","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.fsi.2018.10.010_bib1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1111\/j.1600-065X.2011.01040.x","article-title":"Innate and adaptive immune responses to cell death","volume":"243","author":"Rock","year":"2011","journal-title":"Immunol. Rev."},{"issue":"1","key":"10.1016\/j.fsi.2018.10.010_bib2","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/cr.2009.139","article-title":"Complement and its role in innate and adaptive immune responses","volume":"20","author":"Dunkelberger","year":"2010","journal-title":"Cell Res."},{"issue":"8","key":"10.1016\/j.fsi.2018.10.010_bib3","doi-asserted-by":"crossref","first-page":"3357","DOI":"10.1073\/pnas.93.8.3357","article-title":"Markedly impaired humoral immune response in mice deficient in complement receptors 1 and 2","volume":"93","author":"Molina","year":"1996","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"7","key":"10.1016\/j.fsi.2018.10.010_bib4","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1002793","article-title":"The lectin pathway of complement activation is a critical component of the innate immune response to pneumococcal infection","volume":"8","author":"Ali","year":"2012","journal-title":"PLoS Pathog."},{"issue":"5","key":"10.1016\/j.fsi.2018.10.010_bib5","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1038\/nri800","article-title":"Evolution of the lectin-complement pathway and its role in innate immunity","volume":"2","author":"Fujita","year":"2002","journal-title":"Nat. Rev. Immunol."},{"issue":"3","key":"10.1016\/j.fsi.2018.10.010_bib6","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1046\/j.1365-2249.1998.00663.x","article-title":"Complement regulation in innate immunity and the acute-phase response: inhibition of mannan-binding lectin-initiated complement cytolysis by C-reactive protein (CRP)","volume":"113","author":"Suankratay","year":"1998","journal-title":"Clin. Exp. Immunol."},{"key":"10.1016\/j.fsi.2018.10.010_bib7","doi-asserted-by":"crossref","first-page":"257","DOI":"10.3389\/fimmu.2015.00257","article-title":"Complement system Part II: role in immunity","volume":"6","author":"Merle","year":"2015","journal-title":"Front. Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2018.10.010_bib8","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1046\/j.1365-2567.2000.00009.x","article-title":"The role of complement in the acquired immune response","volume":"100","author":"Nielsen","year":"2000","journal-title":"Immunology"},{"issue":"3","key":"10.1016\/j.fsi.2018.10.010_bib9","doi-asserted-by":"crossref","first-page":"715","DOI":"10.2353\/ajpath.2007.070166","article-title":"The role of complement in inflammatory diseases from behind the scenes into the spotlight","volume":"171","author":"Markiewski","year":"2007","journal-title":"Am. J. Pathol."},{"issue":"11","key":"10.1016\/j.fsi.2018.10.010_bib10","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1016\/j.molimm.2003.10.002","article-title":"Carboxypeptidase N: a pleiotropic regulator of inflammation","volume":"40","author":"Matthews","year":"2004","journal-title":"Mol. Immunol."},{"issue":"15","key":"10.1016\/j.fsi.2018.10.010_bib11","doi-asserted-by":"crossref","first-page":"4618","DOI":"10.1073\/pnas.79.15.4618","article-title":"Isolation and characterization of the subunits of human plasma carboxypeptidase N (kininase i)","volume":"79","author":"Levin","year":"1982","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"6","key":"10.1016\/j.fsi.2018.10.010_bib12","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.semnephrol.2013.08.001","article-title":"Overview of complement activation and regulation","volume":"33","author":"Noris","year":"2013","journal-title":"Semin. Nephrol."},{"issue":"2","key":"10.1016\/j.fsi.2018.10.010_bib13","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1556\/EuJMI.2.2012.2.2","article-title":"The complement system: history, pathways, cascade and inhibitors","volume":"2","author":"Nesargikar","year":"2012","journal-title":"Eur. J. Microbiol. Immunol. (Bp)"},{"issue":"3","key":"10.1016\/j.fsi.2018.10.010_bib14","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1021\/pr050046x","article-title":"Direct tandem mass spectrometry reveals limitations in protein profiling experiments for plasma biomarker discovery","volume":"4","author":"Koomen","year":"2005","journal-title":"J. Proteome Res."},{"issue":"14","key":"10.1016\/j.fsi.2018.10.010_bib15","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1016\/j.intimp.2007.07.014","article-title":"Structure and function of human plasma carboxypeptidase N, the anaphylatoxin inactivator","volume":"7","author":"Skidgel","year":"2007","journal-title":"Int. Immunopharm."},{"issue":"8","key":"10.1016\/j.fsi.2018.10.010_bib16","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/0165-6147(88)90015-6","article-title":"Basic carboxypeptidases: regulators of peptide hormone activity","volume":"9","author":"Skidgel","year":"1988","journal-title":"Trends Pharmacol. Sci."},{"issue":"2","key":"10.1016\/j.fsi.2018.10.010_bib17","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.imbio.2013.08.007","article-title":"Carboxypeptidase N-deficient mice present with polymorphic disease phenotypes on induction of experimental autoimmune encephalomyelitis","volume":"219","author":"Hu","year":"2014","journal-title":"Immunobiology"},{"issue":"3","key":"10.1016\/j.fsi.2018.10.010_bib18","doi-asserted-by":"crossref","first-page":"443","DOI":"10.7326\/0003-4819-93-3-443","article-title":"Familial carboxypeptidase N deficiency","volume":"93","author":"Mathews","year":"1980","journal-title":"Ann. Intern. Med."},{"issue":"10","key":"10.1016\/j.fsi.2018.10.010_bib19","doi-asserted-by":"crossref","first-page":"6533","DOI":"10.4049\/jimmunol.0804207","article-title":"Targeted disruption of the gene encoding the murine small subunit of carboxypeptidase N (CPN1) causes susceptibility to C5a anaphylatoxin-mediated shock","volume":"182","author":"Mueller-Ortiz","year":"2009","journal-title":"J. Immunol."},{"issue":"6","key":"10.1016\/j.fsi.2018.10.010_bib20","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/j.dci.2003.10.005","article-title":"Expression of the third complement component (C3) and carboxypeptidase N small subunit (CPN1) during mouse embryonic development","volume":"28","author":"Matthews","year":"2004","journal-title":"Dev. Comp. Immunol."},{"issue":"16","key":"10.1016\/j.fsi.2018.10.010_bib21","doi-asserted-by":"crossref","first-page":"3435","DOI":"10.1093\/bioinformatics\/bti537","article-title":"FoldIndex: a simple tool to predict whether a given protein sequence is intrinsically unfolded","volume":"21","author":"Prilusky","year":"2005","journal-title":"Bioinformatics"},{"issue":"1","key":"10.1016\/j.fsi.2018.10.010_bib22","first-page":"65","article-title":"Evaluation of environmental estrogenic activities in the Yangtze river estuary: utilization of vitellogenin production in red lip mullet","volume":"59","author":"Meiqin","year":"2011","journal-title":"Aquacult. Sci."},{"issue":"4","key":"10.1016\/j.fsi.2018.10.010_bib23","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"issue":"2","key":"10.1016\/j.fsi.2018.10.010_bib24","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1111\/j.1365-2761.2011.01333.x","article-title":"Nutrition and health of aquaculture fish","volume":"35","author":"Oliva-Teles","year":"2012","journal-title":"J. Fish. Dis."},{"issue":"2","key":"10.1016\/j.fsi.2018.10.010_bib25","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.4049\/jimmunol.134.2.1115","article-title":"Microvascular effects of anaphylatoxins C3a and C5a","volume":"134","author":"Bjork","year":"1985","journal-title":"J. Immunol."},{"issue":"3","key":"10.1016\/j.fsi.2018.10.010_bib26","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1172\/JCI109527","article-title":"Anaphylatoxin-induced histamine release with human leukocytes: studies of C3a leukocyte binding and histamine release","volume":"64","author":"Glovsky","year":"1979","journal-title":"J. Clin. Invest."},{"issue":"7","key":"10.1016\/j.fsi.2018.10.010_bib27","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0002560","article-title":"The complement anaphylatoxin C5a induces apoptosis in adrenomedullary cells during experimental sepsis","volume":"3","author":"Flierl","year":"2008","journal-title":"PloS One"},{"issue":"12","key":"10.1016\/j.fsi.2018.10.010_bib28","doi-asserted-by":"crossref","first-page":"2427","DOI":"10.1172\/JCI106462","article-title":"Anaphylatoxin inactivator of human plasma: its isolation and characterization as a carboxypeptidase","volume":"49","author":"Bokisch","year":"1970","journal-title":"J. Clin. Invest."},{"issue":"3\u20134","key":"10.1016\/j.fsi.2018.10.010_bib29","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.vetimm.2014.10.005","article-title":"Two carboxypeptidase counterparts from rock bream (Oplegnathus fasciatus): molecular characterization, genomic arrangement and immune responses upon pathogenic stresses","volume":"162","author":"Godahewa","year":"2014","journal-title":"Vet. Immunol. Immunopathol."},{"issue":"19","key":"10.1016\/j.fsi.2018.10.010_bib30","doi-asserted-by":"crossref","first-page":"16177","DOI":"10.1074\/jbc.M011457200","article-title":"The crystal structure of the inhibitor-complexed carboxypeptidase D domain II and the modeling of regulatory carboxypeptidases","volume":"276","author":"Aloy","year":"2001","journal-title":"J. Biol. Chem."},{"issue":"2","key":"10.1016\/j.fsi.2018.10.010_bib31","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/j.jmb.2006.11.025","article-title":"Crystal structure of the human carboxypeptidase N (kininase I) catalytic domain","volume":"366","author":"Keil","year":"2007","journal-title":"J. Mol. Biol."},{"issue":"2","key":"10.1016\/j.fsi.2018.10.010_bib32","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.bbrc.2012.09.081","article-title":"Binding of carboxypeptidase N to fibrinogen and fibrin","volume":"427","author":"Talens","year":"2012","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"10.1016\/j.fsi.2018.10.010_bib33","doi-asserted-by":"crossref","first-page":"262","DOI":"10.3389\/fimmu.2015.00262","article-title":"Complement system Part I - molecular mechanisms of activation and regulation","volume":"6","author":"Merle","year":"2015","journal-title":"Front. Immunol."},{"issue":"6","key":"10.1016\/j.fsi.2018.10.010_bib34","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1016\/j.sbi.2008.10.002","article-title":"Function and structure of inherently disordered proteins","volume":"18","author":"Dunker","year":"2008","journal-title":"Curr. Opin. Struct. Biol."},{"issue":"2","key":"10.1016\/j.fsi.2018.10.010_bib35","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1006\/jmbi.1999.3110","article-title":"Intrinsically unstructured proteins: re-assessing the protein structure-function paradigm","volume":"293","author":"Wright","year":"1999","journal-title":"J. Mol. Biol."},{"issue":"7","key":"10.1016\/j.fsi.2018.10.010_bib36","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1074\/mcp.M700564-MCP200","article-title":"Phosphoproteomic analysis of the mouse brain cytosol reveals a predominance of protein phosphorylation in regions of intrinsic sequence disorder","volume":"7","author":"Collins","year":"2008","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.fsi.2018.10.010_bib37","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1146\/annurev.me.37.020186.000405","article-title":"The role of the spleen in resistance to infection","volume":"37","author":"Bohnsack","year":"1986","journal-title":"Annu. Rev. Med."},{"issue":"2","key":"10.1016\/j.fsi.2018.10.010_bib38","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1002\/jmor.1051790205","article-title":"Phagocytosis in the spleen of the sunfish Lepomis spp","volume":"179","author":"Fulop","year":"1984","journal-title":"J. Morphol."},{"issue":"5","key":"10.1016\/j.fsi.2018.10.010_bib39","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1084\/jem.123.5.777","article-title":"Effect of splenectomy on the susceptibility of mice inoculated with Diplococcus pneumoniae","volume":"123","author":"Shinefield","year":"1966","journal-title":"J. Exp. Med."},{"issue":"14","key":"10.1016\/j.fsi.2018.10.010_bib40","doi-asserted-by":"crossref","first-page":"R548","DOI":"10.1016\/j.cub.2011.06.005","article-title":"Inflammation: cell recruitment versus local proliferation","volume":"21","author":"Helming","year":"2011","journal-title":"Curr. Biol."},{"issue":"2","key":"10.1016\/j.fsi.2018.10.010_bib41","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1128\/CMR.00046-08","article-title":"Pathogen recognition and inflammatory signaling in innate immune defenses","volume":"22","author":"Mogensen","year":"2009","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.fsi.2018.10.010_bib42","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s12929-015-0151-1","article-title":"Complement and macrophage crosstalk during process of angiogenesis in tumor progression","volume":"22","author":"Khan","year":"2015","journal-title":"J. Biomed. Sci."},{"issue":"10","key":"10.1016\/j.fsi.2018.10.010_bib43","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1038\/ni.2705","article-title":"Tissue-resident macrophages","volume":"14","author":"Davies","year":"2013","journal-title":"Nat. Immunol."},{"issue":"6","key":"10.1016\/j.fsi.2018.10.010_bib44","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.1016\/j.jhep.2017.02.026","article-title":"Targeting hepatic macrophages to treat liver diseases","volume":"66","author":"Tacke","year":"2017","journal-title":"J. Hepatol."},{"key":"10.1016\/j.fsi.2018.10.010_bib45","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.rinim.2014.01.001","article-title":"Characterization of the liver-macrophages isolated from a mixed primary culture of neonatal swine hepatocytes","volume":"4","author":"Kitani","year":"2014","journal-title":"Results Immunol."},{"issue":"5","key":"10.1016\/j.fsi.2018.10.010_bib46","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1016\/j.immuni.2013.10.010","article-title":"The spleen in local and systemic regulation of immunity","volume":"39","author":"Bronte","year":"2013","journal-title":"Immunity"},{"issue":"3","key":"10.1016\/j.fsi.2018.10.010_bib47","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1513\/pats.200704-046AW","article-title":"Complement activation pathways: a bridge between innate and adaptive immune responses in asthma","volume":"4","author":"Wills-Karp","year":"2007","journal-title":"Proc. Am. Thorac. Soc."},{"issue":"1","key":"10.1016\/j.fsi.2018.10.010_bib48","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1128\/CMR.00022-07","article-title":"Sensing of viral infection and activation of innate immunity by toll-like receptor 3","volume":"21","author":"Vercammen","year":"2008","journal-title":"Clin. Microbiol. Rev."},{"issue":"4","key":"10.1016\/j.fsi.2018.10.010_bib49","doi-asserted-by":"crossref","first-page":"R759","DOI":"10.1152\/ajpregu.00293.2004","article-title":"The viral mimic, polyinosinic:polycytidylic acid, induces fever in rats via an interleukin-1-dependent mechanism","volume":"287","author":"Fortier","year":"2004","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"10.1016\/j.fsi.2018.10.010_bib50","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1046\/j.1365-2761.2002.00415.x","article-title":"Lactococcus garvieae, a cause of disease in grey mullet, Mugil cephalus L., in Taiwan","volume":"25","author":"Chen","year":"2002","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2018.10.010_bib51","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/j.fsi.2016.10.006","article-title":"Transcriptome analysis of grey mullet (Mugil cephalus) after challenge with Lactococcus garvieae","volume":"58","author":"Byadgi","year":"2016","journal-title":"Fish Shellfish Immunol."},{"issue":"13","key":"10.1016\/j.fsi.2018.10.010_bib52","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1096\/fasebj.9.13.7557022","article-title":"Molecular mechanisms and therapeutic strategies related to nitric oxide","volume":"9","author":"Moncada","year":"1995","journal-title":"Faseb. J."},{"issue":"Pt 1","key":"10.1016\/j.fsi.2018.10.010_bib53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/bj3360001","article-title":"Arginine metabolism: nitric oxide and beyond","volume":"336","author":"Wu","year":"1998","journal-title":"Biochem. J."},{"issue":"4","key":"10.1016\/j.fsi.2018.10.010_bib54","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1177\/014107689909200402","article-title":"Nitric oxide: discovery and impact on clinical medicine","volume":"92","author":"Moncada","year":"1999","journal-title":"J. R. Soc. Med."},{"key":"10.1016\/j.fsi.2018.10.010_bib55","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1146\/annurev.bi.63.070194.001135","article-title":"Nitric oxide: a physiologic messenger molecule","volume":"63","author":"Bredt","year":"1994","journal-title":"Annu. Rev. Biochem."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S105046481830634X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S105046481830634X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T18:14:19Z","timestamp":1693937659000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S105046481830634X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1]]},"references-count":55,"alternative-id":["S105046481830634X"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2018.10.010","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2019,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification and characterization of a carboxypeptidase N1 from red lip mullet (Liza haematocheila); revealing its immune relevance","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2018.10.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}