{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:58:39Z","timestamp":1786989519330,"version":"build-2736575974"},"reference-count":36,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"crossref","award":["2011-137"],"award-info":[{"award-number":["2011-137"]}],"id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2019,3]]},"DOI":"10.1016\/j.fsi.2018.12.054","type":"journal-article","created":{"date-parts":[[2018,12,27]],"date-time":"2018-12-27T20:27:48Z","timestamp":1545942468000},"page":"1114-1122","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"special_numbering":"C","title":["MDA5 and LGP2 acts as a key regulator though activating NF-\u03baB and IRF3 in RLRs signaling of mandarinfish"],"prefix":"10.1016","volume":"86","author":[{"given":"Tiantian","family":"Gu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bowen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhi","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinsheng","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2791-0429","authenticated-orcid":false,"given":"Qi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.fsi.2018.12.054_bib1","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/j.immuni.2007.08.012","article-title":"Innate recognition of viruses","volume":"27","author":"Pichlmair","year":"2007","journal-title":"Immunity"},{"issue":"5","key":"10.1016\/j.fsi.2018.12.054_bib2","first-page":"342","article-title":"Pattern recognition and signaling mechanisms of RIG-I and MDA5","volume":"5","author":"Stephanie","year":"2014","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2018.12.054_bib3","doi-asserted-by":"crossref","unstructured":"T. Kawai, S. Akira, Toll-like Receptor and RIG-I-like Receptor Signaling, Cold Spring Harbor Laboratory Press2008.","DOI":"10.1196\/annals.1443.020"},{"issue":"1","key":"10.1016\/j.fsi.2018.12.054_bib4","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.coi.2008.01.002","article-title":"MDA5\/RIG-I and virus recognition","volume":"20","author":"Takeuchi","year":"2008","journal-title":"Curr Opin Immunol"},{"issue":"5801","key":"10.1016\/j.fsi.2018.12.054_bib5","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1126\/science.1132998","article-title":"Mediated antiviral responses to single-stranded RNA bearing 5\u2032-phosphates","volume":"314","author":"Pichlmair","year":"2006","journal-title":"Science (New York, N.Y.)"},{"issue":"26","key":"10.1016\/j.fsi.2018.12.054_bib6","doi-asserted-by":"crossref","first-page":"17465","DOI":"10.1074\/jbc.M109.007179","article-title":"Solution structures of cytosolic RNA sensor MDA5 and LGP2 C-terminal domains: identification of the RNA recognition loop in RIG-I-like receptors","volume":"284","author":"T","year":"2009","journal-title":"J. Biol. Chem."},{"issue":"7","key":"10.1016\/j.fsi.2018.12.054_bib7","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1038\/ni1087","article-title":"The RNA helicase RIG-I has an essential function in double-stranded RNA-induced innate antiviral responses","volume":"5","author":"Yoneyama","year":"2004","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.fsi.2018.12.054_bib8","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.coi.2014.12.012","article-title":"Viral RNA detection by RIG-I-like receptors","volume":"32","author":"Yoneyama","year":"2015","journal-title":"Curr. Opin. Immunol."},{"issue":"6","key":"10.1016\/j.fsi.2018.12.054_bib9","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/j.molcel.2005.08.014","article-title":"VISA is an adapter protein required for virus-triggered IFN-beta signaling","volume":"19","author":"Han","year":"2005","journal-title":"Mol. Cell"},{"issue":"3","key":"10.1016\/j.fsi.2018.12.054_bib10","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1016\/j.cyto.2008.07.011","article-title":"Negative regulation of cytoplasmic RNA-mediated antiviral signaling","volume":"43","author":"Komuro","year":"2008","journal-title":"Cytokine"},{"issue":"24","key":"10.1016\/j.fsi.2018.12.054_bib11","doi-asserted-by":"crossref","first-page":"12332","DOI":"10.1128\/JVI.01325-06","article-title":"RNA- and virus-independent inhibition of antiviral signaling by RNA helicase LGP2","volume":"80","author":"Komuro","year":"2006","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2018.12.054_bib12","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.fsi.2016.04.001","article-title":"Antiviral function of grouper MDA5 against iridovirus and nodavirus","volume":"54","author":"Huang","year":"2016","journal-title":"Fish Shellfish Immunol."},{"issue":"39","key":"10.1016\/j.fsi.2018.12.054_bib13","doi-asserted-by":"crossref","first-page":"10950","DOI":"10.1073\/pnas.1604939113","article-title":"Loss of RIG-I leads to a functional replacement with MDA5 in the Chinese tree shrew","volume":"113","author":"Xu","year":"2016","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"4","key":"10.1016\/j.fsi.2018.12.054_bib14","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1073\/pnas.0912986107","article-title":"LGP2 is a positive regulator of RIG-I- and MDA5-mediated antiviral responses","volume":"107","author":"Satoh","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.fsi.2018.12.054_bib15","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/j.fsi.2016.06.032","article-title":"Molecular characterization and expression analyses of three RIG-I-like receptor signaling pathway genes (MDA5, LGP2 and MAVS) in Larimichthys crocea","volume":"55","author":"Shen","year":"2016","journal-title":"Fish Shellfish Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2018.12.054_bib16","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1186\/1471-2148-9-85","article-title":"Origin and evolution of the RIG-I like RNA helicase gene family","volume":"9","author":"Zou","year":"2009","journal-title":"BMC Evol. Biol."},{"issue":"5","key":"10.1016\/j.fsi.2018.12.054_bib17","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1016\/j.fsi.2012.08.015","article-title":"Differential expression analysis of nuclear oligomerization domain proteins NOD1 and NOD2 in orange-spotted grouper (Epinephelus coioides)","volume":"33","author":"Hou","year":"2012","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2018.12.054_bib18","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkl031","article-title":"WEGO: a web tool for plotting GO annotations","volume":"34","author":"Ye","year":"2006","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"10.1016\/j.fsi.2018.12.054_bib19","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1186\/1471-2164-14-601","article-title":"Insights into food preference in hybrid F1 of Siniperca chuatsi (\u2640)\u2009\u00d7\u2009 Siniperca scherzeri (\u2642) Mandarin fish through transcriptome analysis","volume":"14","author":"He","year":"2013","journal-title":"BMC Genomics"},{"issue":"6","key":"10.1016\/j.fsi.2018.12.054_bib20","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1038\/nprot.2008.73","article-title":"Livak KJAnalyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.fsi.2018.12.054_bib21","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.mib.2014.05.011","article-title":"Sensing viral invasion by RIG-I like receptors","volume":"20","author":"Yoo","year":"2014","journal-title":"Curr. Opin. Microbiol."},{"issue":"6","key":"10.1016\/j.fsi.2018.12.054_bib22","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1016\/j.fsi.2013.02.005","article-title":"Innate immunity of finfish: primordial conservation and function of viral RNA sensors in teleosts","volume":"35","author":"Aoki","year":"2013","journal-title":"Fish Shellfish Immunol."},{"issue":"4","key":"10.1016\/j.fsi.2018.12.054_bib23","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/j.fsi.2010.01.009","article-title":"Molecular cloning and immune responsive expression of MDA5 gene, a pivotal member of the RLR gene family from grass carp Ctenopharyngodon idella","volume":"28","author":"Su","year":"2010","journal-title":"Fish Shellfish Immunol."},{"issue":"2","key":"10.1016\/j.fsi.2018.12.054_bib24","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.fsi.2010.04.001","article-title":"Identification and expression profiling analysis of grass carp Ctenopharyngodon idella LGP2 cDNA","volume":"29","author":"Teng","year":"2010","journal-title":"Fish Shellfish Immunol."},{"issue":"3\u20134","key":"10.1016\/j.fsi.2018.12.054_bib25","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.dci.2012.02.004","article-title":"Pathogen recognition receptors in channel catfish: II. Identification, phylogeny and expression of retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs)","volume":"37","author":"Rajendran","year":"2012","journal-title":"Dev. Comp. Immunol."},{"issue":"5","key":"10.1016\/j.fsi.2018.12.054_bib26","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.dci.2010.12.013","article-title":"Characterization and antiviral function of a cytosolic sensor gene, MDA5, in Japanese flounder, Paralichthys olivaceus","volume":"35","author":"Ohtani","year":"2011","journal-title":"Dev. Comp. Immunol."},{"issue":"12","key":"10.1016\/j.fsi.2018.12.054_bib27","doi-asserted-by":"crossref","first-page":"7507","DOI":"10.4049\/jimmunol.1001850","article-title":"Evolutional conservation of molecular structure and antiviral function of a viral RNA receptor, LGP2, in Japanese flounder, Paralichthys olivaceus","volume":"185","author":"Ohtani","year":"2010","journal-title":"J. Immunol."},{"issue":"7","key":"10.1016\/j.fsi.2018.12.054_bib28","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1111\/j.1462-5822.2010.01478.x","article-title":"Induction of type I interferons by bacteria","volume":"12","author":"Monroe","year":"2010","journal-title":"Cell Microbiol."},{"issue":"5","key":"10.1016\/j.fsi.2018.12.054_bib29","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1016\/j.immuni.2011.05.003","article-title":"Immune signaling by RIG-I-like receptors","volume":"34","author":"Loo","year":"2011","journal-title":"Immunity"},{"issue":"8","key":"10.1016\/j.fsi.2018.12.054_bib30","doi-asserted-by":"crossref","first-page":"5260","DOI":"10.4049\/jimmunol.175.8.5260","article-title":"The RNA helicase Lgp2 inhibits TLR-independent sensing of viral replication by retinoic acid-inducible gene-I","volume":"175","author":"Rothenfusser","year":"2005","journal-title":"J. Immunol."},{"issue":"16","key":"10.1016\/j.fsi.2018.12.054_bib31","doi-asserted-by":"crossref","first-page":"8403","DOI":"10.1128\/JVI.00445-10","article-title":"Expression and functional characterization of the RIG-I-like receptors MDA5 and LGP2 in Rainbow trout (Oncorhynchus mykiss)","volume":"85","author":"Chang","year":"2011","journal-title":"J. Virol."},{"issue":"12","key":"10.1016\/j.fsi.2018.12.054_bib32","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0051522","article-title":"LGP2 Expression is Enhanced by Interferon Regulatory Factor 3 in Olive Flounder, Paralichthys olivaceus","volume":"7","author":"Hikima","year":"2012","journal-title":"PLoS One"},{"issue":"4","key":"10.1016\/j.fsi.2018.12.054_bib33","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0018842","article-title":"Impaired cellular responses to cytosolic DNA or infection with Listeria monocytogenes and vaccinia virus in the absence of the murine LGP2 protein","volume":"6","author":"Pollpeter","year":"2011","journal-title":"PLoS One"},{"issue":"5","key":"10.1016\/j.fsi.2018.12.054_bib34","doi-asserted-by":"crossref","first-page":"2851","DOI":"10.4049\/jimmunol.175.5.2851","article-title":"Shared and unique functions of the DExD\/H-box helicases RIG-I, MDA5, and LGP2 in antiviral innate immunity","volume":"175","author":"Yoneyama","year":"2005","journal-title":"J. Immunol."},{"issue":"10","key":"10.1016\/j.fsi.2018.12.054_bib35","doi-asserted-by":"crossref","first-page":"6444","DOI":"10.4049\/jimmunol.178.10.6444","article-title":"Loss of DExD\/H box RNA helicase LGP2 manifests disparate antiviral responses","volume":"178","author":"Venkataraman","year":"2007","journal-title":"J. Immunol."},{"key":"10.1016\/j.fsi.2018.12.054_bib36","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.fsi.2016.07.015","article-title":"Negative regulation of the antiviral response by grouper LGP2 against fish viruses","volume":"56","author":"Yu","year":"2016","journal-title":"Fish Shellfish Immunol."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S105046481830860X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S105046481830860X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,20]],"date-time":"2020-05-20T12:47:44Z","timestamp":1589978864000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S105046481830860X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3]]},"references-count":36,"alternative-id":["S105046481830860X"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2018.12.054","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2019,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"MDA5 and LGP2 acts as a key regulator though activating NF-\u03baB and IRF3 in RLRs signaling of mandarinfish","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2018.12.054","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}