{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T20:34:08Z","timestamp":1770755648306,"version":"3.50.0"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T00:00:00Z","timestamp":1564617600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T00:00:00Z","timestamp":1564617600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2019,8]]},"DOI":"10.1016\/j.fsi.2019.05.014","type":"journal-article","created":{"date-parts":[[2019,5,13]],"date-time":"2019-05-13T11:15:40Z","timestamp":1557746140000},"page":"68-77","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"special_numbering":"C","title":["Expression and functional characterization of a mannose-binding lectin-associated serine protease-1 (MASP-1) from Nile tilapia (Oreochromis niloticus) in host defense against bacterial infection"],"prefix":"10.1016","volume":"91","author":[{"given":"Liangliang","family":"Mu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxue","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hairong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kailiang","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liting","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingmei","family":"Ding","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Bian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingxi","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengli","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zheng","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianmin","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2019.05.014_bib1","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1038\/ni.1923","article-title":"Complement: a key system for immune surveillance and homeostasis","volume":"11","author":"Ricklin","year":"2010","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib2","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.molimm.2015.03.245","article-title":"MBL-associated serine proteases (MASPs) and infectious diseases","volume":"67","author":"Beltrame","year":"2015","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib3","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/cr.2009.139","article-title":"Complement and its role in innate and adaptive immune responses","volume":"20","author":"Dunkelberger","year":"2010","journal-title":"Cell Res."},{"key":"10.1016\/j.fsi.2019.05.014_bib4","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1016\/j.molimm.2015.02.014","article-title":"MASP-1 of the complement system promotes clotting via prothrombin activation","volume":"65","author":"Jenny","year":"2015","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib5","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.molimm.2013.05.007","article-title":"Toward a structure-based compre-hension of the lectin pathway of complement","volume":"56","author":"Kjaer","year":"2013","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0087104","article-title":"MASP-1 induces a unique cytokine pattern in endothelial cells: a novel link between complement system and neutrophil granulocytes","volume":"9","author":"Jani","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2019.05.014_bib7","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.4049\/jimmunol.164.5.2281","article-title":"Cutting edge: complement activating complex of ficolin and mannose-binding lectin-associated serine protease","volume":"164","author":"Matsushita","year":"2000","journal-title":"J. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib8","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1111\/j.1365-2249.2012.04584.x","article-title":"Mannan-binding lectin (MBL)-associated serine protease-1 (MASP-1), a serine protease associated with humoral pattern-recognition molecules: normal and acute-phase levels in serum and stoichiometry of lectin pathway components","volume":"169","author":"Thiel","year":"2012","journal-title":"Clin. Exp. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib9","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.fsi.2017.06.016","article-title":"Identification and characterization of a mannose-binding lectin from Nile tilapia (Oreochromis niloticus)","volume":"67","author":"Mu","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib10","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1084\/jem.176.6.1497","article-title":"Activation of the classical complement pathway by mannose-binding protein in association with a novel C1s-like serine protease","volume":"176","author":"Matsushita","year":"1992","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.fsi.2019.05.014_bib11","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.4049\/jimmunol.0901141","article-title":"MASP-1, a promiscuous complement protease: structure of its catalytic region reveals the basis of its broad specificity","volume":"183","author":"Dob\u00f3","year":"2009","journal-title":"J. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib12","doi-asserted-by":"crossref","first-page":"3957","DOI":"10.4049\/jimmunol.1201736","article-title":"Mannan-binding lectin-associated serine protease (MASP)-1 is crucial for lectin pathway activation in human serum, whereas neither MASP-1 nor MASP- 3 is required for alternative pathway function","volume":"189","author":"Degn","year":"2012","journal-title":"J. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib13","doi-asserted-by":"crossref","first-page":"10498","DOI":"10.1073\/pnas.1202588109","article-title":"Revised mechanism of complement lectin-pathway activation revealing the role of serine protease MASP-1 as the exclusive activator of MASP-2","volume":"109","author":"H\u00e9ja","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.fsi.2019.05.014_bib14","doi-asserted-by":"crossref","first-page":"1294","DOI":"10.1016\/j.bbapap.2008.03.020","article-title":"The action of MBL-associated serine protease 1 (MASP1) on factor XIII and fibrinogen","volume":"1784","author":"Krarup","year":"2008","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.fsi.2019.05.014_bib15","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.molimm.2014.05.013","article-title":"Multiple roles of complement MASP-1 at the interface of innate immune response and coagulation","volume":"61","author":"Dob\u00f3","year":"2014","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib16","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.fsi.2017.05.020","article-title":"Mannan-binding lectin-associated serine protease-1 (MASP-1) mediates immune responses against Aeromonas hydrophila in vitro and in vivo in grass carp","volume":"66","author":"Dang","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib17","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.fsi.2018.01.023","article-title":"Expression and functional characterization of transferrin in Nile tilapia (Oreochromis niloticus) in response to bacterial infection","volume":"74","author":"Yin","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib18","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.dci.2018.02.015","article-title":"A C-type lectin (CL11X1-like) from Nile tilapia (Oreochromis niloticus) is involved in host defense against bacterial infection","volume":"84","author":"Mu","year":"2018","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib19","doi-asserted-by":"crossref","first-page":"5132","DOI":"10.1128\/JB.01080-12","article-title":"Complete genome sequence of Streptococcus agalactiae ZQ0910, a pathogen causing meningoencephalitis in the GIFT strain of Nile tilapia (Oreochromis niloticus)","volume":"194","author":"Wang","year":"2012","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.fsi.2019.05.014_bib20","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.fsi.2014.05.028","article-title":"Immunostimulatory effects of artificial feed supplemented with a Chinese herbal mixture on Oreochromis niloticus against Aeromonas hydrophila","volume":"39","author":"Tang","year":"2014","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib21","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.dci.2014.05.003","article-title":"Functional characterization of mannose-binding lectin in zebrafish: implication for a lectin-dependent complement system in early embryos","volume":"46","author":"Yang","year":"2014","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib22","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2019.05.014_bib23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1290\/0410068.1","article-title":"Establishment and comparative of different culture conditions of primary hepatocytes from Nile tilapia (Oreochromis niloticus) as a model to study stress induction in vitro","volume":"41","author":"Jung\u2019a","year":"2005","journal-title":"In Vitro Cell. Dev. Biol. Anim."},{"issue":"2","key":"10.1016\/j.fsi.2019.05.014_bib24","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.aquatox.2006.07.021","article-title":"Primary cultured cells as sensitive in vitro model for assessment of toxicants-comparison to hepatocytes and gill epithelia","volume":"80","author":"Zhou","year":"2006","journal-title":"Aquat. Toxicol."},{"key":"10.1016\/j.fsi.2019.05.014_bib25","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1006\/fsim.2001.0372","article-title":"Antibody increases phagocytosis and killing of Lactococcus garvieae by rainbow trout (Oncorhynchus mykiss, L.) macrophages","volume":"12","author":"Barnes","year":"2002","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib26","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1016\/j.fsi.2010.08.004","article-title":"The iron-cofactored superoxide dismutase of Edwardsiella tarda inhibits macrophage-mediated innate immune response","volume":"29","author":"Cheng","year":"2010","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib27","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1007\/s10126-010-9315-x","article-title":"Lipovitellin is a non-self recognition receptor with opsonic activity","volume":"13","author":"Zhang","year":"2011","journal-title":"Mar. Biotechnol."},{"key":"10.1016\/j.fsi.2019.05.014_bib28","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.molimm.2018.01.004","article-title":"Immune modulatory properties of 6-gingerol and resveratrol in Atlantic salmon macrophages","volume":"95","author":"Smith","year":"2018","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib29","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.fsi.2015.03.023","article-title":"Dualparallel inhibition of IL-10 and TGF-\u03b21 controls LPS-induced inflammatory response via NF-\u03baB signaling in grass carp monocytes\/macrophages","volume":"44","author":"Wei","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib30","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.dci.2018.08.012","article-title":"Interleukin-6 gets involved in response to bacterial infection and promotes antibody production in Nile tilapia (Oreochromis niloticus)","volume":"89","author":"Wei","year":"2018","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib31","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.molimm.2018.08.012","article-title":"Expression and functional characterization of collection-K1 from Nile tilapia (Oreochromis niloticus) in host innate immune defense","volume":"103","author":"Mu","year":"2018","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib32","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.fsi.2016.12.014","article-title":"Identification and characterization of C1 inhibitor in Nile tilapia (Oreochromis niloticus) in response to pathogenic bacteria","volume":"61","author":"Ding","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib33","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1111\/imr.12460","article-title":"The emerging roles of mannose-binding lectin-associated serine proteases (MASPs) in the lectin pathway of complement and beyond","volume":"274","author":"Dobo","year":"2016","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.fsi.2019.05.014_bib34","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.1093\/emboj\/cdg236","article-title":"Crystal structure of the CUB1-EGF-CUB2 region of mannose-binding protein associated serine protease-2","volume":"10","author":"Feinberg","year":"2003","journal-title":"EMBO J."},{"issue":"8","key":"10.1016\/j.fsi.2019.05.014_bib35","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1038\/nri1669","article-title":"Structure and function of the spleen","volume":"5","author":"Mebius","year":"2005","journal-title":"Nat. Rev. Immunol."},{"issue":"5","key":"10.1016\/j.fsi.2019.05.014_bib36","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1080\/01926230600867743","article-title":"Normal structure, function, and histology of the spleen","volume":"34","author":"Cesta","year":"2006","journal-title":"Toxicol. Pathol."},{"key":"10.1016\/j.fsi.2019.05.014_bib37","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/j.fsi.2015.07.026","article-title":"Characterization of rock bream (Oplegnathus fasciatus) complement components C1r and C1s in terms of molecular aspects, genomic modulation, and immune responsive transcriptional profiles following bacterial and viral pathogen exposure","volume":"46","author":"Godahewa","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib38","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.fsi.2017.09.015","article-title":"C1r and C1s from Nile tilapia (Oreochromis niloticus): molecular characterization, transcriptional profiling upon bacterial and IFN-\u03b3 inductions and potential role in response to bacterial infection","volume":"70","author":"Zhong","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib39","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.immuni.2006.03.002","article-title":"Rewiring phagocytic signal transduction","volume":"24","author":"Lowell","year":"2006","journal-title":"Immunity"},{"key":"10.1016\/j.fsi.2019.05.014_bib40","series-title":"Medical Immunology: Immune Molecule and Immune Cell","first-page":"43","author":"Gong","year":"2014"},{"key":"10.1016\/j.fsi.2019.05.014_bib41","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1111\/cei.12093","article-title":"Plasma levels of mannan-binding lectin (MBL)-associated serine proteases (MASPs) and MBL-associated protein in cardio and cerebrovascular diseases","volume":"173","author":"Frauenknecht","year":"2013","journal-title":"Clin. Exp. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib42","first-page":"281","article-title":"Macrophages in inflammation","volume":"4","author":"Fujiwara","year":"2005","journal-title":"Curr. Drug Targets"},{"key":"10.1016\/j.fsi.2019.05.014_bib43","series-title":"Fish Physiology: the Non-specific Cellular Immune Defense Mechanisms","first-page":"375","author":"Lin","year":"2011"},{"key":"10.1016\/j.fsi.2019.05.014_bib44","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1038\/nri1200","article-title":"Macrophage migration inhibitory factor: a regulator of innate immunity","volume":"3","author":"Calandra","year":"2003","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib45","doi-asserted-by":"crossref","first-page":"3369","DOI":"10.4049\/jimmunol.170.6.3369","article-title":"IL-8 directly enhanced Endothelial cell survival, proliferation, and matrix metalloproteinases production and regulated angiogenesis","volume":"170","author":"Li","year":"2003","journal-title":"J. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib46","doi-asserted-by":"crossref","first-page":"E433","DOI":"10.1152\/ajpendo.00074.2003","article-title":"IL-6 enhances plasma IL-1ra, IL-10, and cortisol in humans","volume":"285","author":"Steensberg","year":"2003","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"10.1016\/j.fsi.2019.05.014_bib47","first-page":"1","article-title":"Autophagy in macrophages: impacting inflammation and bacterial infection","volume":"2014","author":"Vural","year":"2014","journal-title":"Sci. Tech. Rep."},{"key":"10.1016\/j.fsi.2019.05.014_bib48","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1038\/nri2711","article-title":"The regulation of IL-10 production by immune cells","volume":"10","author":"Saraiva","year":"2010","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib49","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1146\/annurev.immunol.22.012703.104543","article-title":"Chemokines in innate and adaptive host defense: basic chemokinese grammar for immune cells","volume":"22","author":"Rot","year":"2004","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.fsi.2019.05.014_bib50","doi-asserted-by":"crossref","first-page":"2591","DOI":"10.1096\/fasebj.6.8.1592209","article-title":"Cytokine regulation of endothelial cell function","volume":"6","author":"Mantovani","year":"1992","journal-title":"FASEB J."},{"key":"10.1016\/j.fsi.2019.05.014_bib51","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1038\/nature12034","article-title":"Macrophage biology in development, homeostasis and disease","volume":"496","author":"Wynn","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2019.05.014_bib52","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1182\/blood-2003-08-2891","article-title":"Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments","volume":"104","author":"Oynebraten","year":"2004","journal-title":"Blood"}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464819305704?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464819305704?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T05:09:17Z","timestamp":1760936957000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464819305704"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8]]},"references-count":52,"alternative-id":["S1050464819305704"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2019.05.014","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2019,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Expression and functional characterization of a mannose-binding lectin-associated serine protease-1 (MASP-1) from Nile tilapia (Oreochromis niloticus) in host defense against bacterial infection","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2019.05.014","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}