{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:11:39Z","timestamp":1761005499013,"version":"build-2065373602"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2020,9]]},"DOI":"10.1016\/j.fsi.2020.05.042","type":"journal-article","created":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T20:49:17Z","timestamp":1590439757000},"page":"101-110","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Cloning, expression profile of the complement component C9 gene and influence of the recombinant C9 protein on peripheral mononuclear leukocytes transcriptome in half-smooth tongue sole (Cynoglossus semilaevis)"],"prefix":"10.1016","volume":"104","author":[{"given":"Jingchao","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yadong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linqing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiyun","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenxia","family":"Sha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2020.05.042_bib1","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1042\/CS20020362","article-title":"Beyond lysis: how complement influences cell fate","volume":"104","author":"Cole","year":"2003","journal-title":"Clin. Sci."},{"key":"10.1016\/j.fsi.2020.05.042_bib2","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.vaccine.2008.11.022","article-title":"Complement and humoral immunity","volume":"26","author":"Carroll","year":"2008","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2020.05.042_bib3","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/cr.2009.139","article-title":"Complement and its role in innate and adaptive immune responses","volume":"20","author":"Dunkelberger","year":"2010","journal-title":"Cell Res."},{"key":"10.1016\/j.fsi.2020.05.042_bib4","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1006\/fsim.2001.0408","article-title":"The complement system in teleosts","volume":"12","author":"Holland","year":"2002","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib5","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/j.imbio.2015.04.006","article-title":"The membrane attack complex as an inflammatory trigger","volume":"221","author":"Morgan","year":"2016","journal-title":"Immunobiology"},{"key":"10.1016\/j.fsi.2020.05.042_bib6","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1385\/IR:31:1:37","article-title":"The role of C5b-9 terminal complement complex in activation of the cell cycle and transcription","volume":"31","author":"Fosbrink","year":"2005","journal-title":"Immunol. Res."},{"key":"10.1016\/j.fsi.2020.05.042_bib7","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1007\/s00251-006-0142-1","article-title":"Genomic view of the evolution of the complement system","volume":"58","author":"Nonaka","year":"2006","journal-title":"Immunogenetics"},{"key":"10.1016\/j.fsi.2020.05.042_bib8","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1016\/j.fsi.2012.02.010","article-title":"An unexpected loss of domains in the conservative evolution ninth complement component in a higher teleost, Miichthys miiuy","volume":"32","author":"Meng","year":"2012","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib9","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s002510050684","article-title":"Molecular analysis of complement component C8\u03b2 and C9 cDNAs of Japanese flounder, Paralichthys olivaceus","volume":"50","author":"Katagiri","year":"1999","journal-title":"Immunogenetics"},{"key":"10.1016\/j.fsi.2020.05.042_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/ijms17030345","article-title":"Molecular characterization and expression analyses of the complement component C8\u03b1, C8\u03b2 and C9 genes in yellow catfish (Pelteobagrus fulvidraco) after the Aeromonas hydrophila challenge","volume":"17","author":"Zheng","year":"2016","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fsi.2020.05.042_bib11","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S0378-1119(97)00423-X","article-title":"Cloning and sequencing of complement component C9 and its linkage to DOC-2 in the pufferfish Fugu rubripes","volume":"200","author":"Yeo","year":"1997","journal-title":"Gene"},{"key":"10.1016\/j.fsi.2020.05.042_bib12","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.vetimm.2007.05.005","article-title":"Molecular cloning, promoter analysis and induced expression of the complement component C9 gene in the grass carp Ctenopharyngodon idella","volume":"118","author":"Li","year":"2007","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.fsi.2020.05.042_bib13","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/0145-305X(93)90016-J","article-title":"Domain structure, functional activity, and polymerization of trout complement protein C9","volume":"17","author":"Tomlinson","year":"1993","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib14","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/S0161-5890(96)00054-5","article-title":"Purification and characterization of the eighth and ninth components of carp complement","volume":"33","author":"Uemura","year":"1996","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib15","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.fsi.2012.06.019","article-title":"Genomic characterization and expression analysis of complement component 9 in rock bream (Oplegnathus fasciatus)","volume":"33","author":"Wickramaarachchi","year":"2012","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib16","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.fsi.2016.01.015","article-title":"Characterisation and expression analysis of two terminal complement components: C7 and C9 from large yellow croaker, Larimichthys crocea","volume":"51","author":"Guo","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib17","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.fsi.2013.04.042","article-title":"Molecular characterization and expression analysis of complement component C9 gene in the whitespotted bambooshark, Chiloscyllium plagiosum","volume":"35","author":"Wang","year":"2013","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib18","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1186\/1471-2164-11-518","article-title":"Zebrafish fin immune responses during high mortality infections with viral haemorrhagic septicemia rhabdovirus. A proteomic and transcriptomic approach","volume":"11","author":"Encinas","year":"2010","journal-title":"BMC Genom."},{"year":"2019","series-title":"China Fishery Statistics Yearbook","author":"Xu","key":"10.1016\/j.fsi.2020.05.042_bib19"},{"key":"10.1016\/j.fsi.2020.05.042_bib20","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1038\/s41598-017-00884-4","article-title":"Identification of exosomes and its signature miRNAs of male and female Cynoglossus semilaevis","volume":"7","author":"Sun","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fsi.2020.05.042_bib21","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1016\/j.fsi.2017.11.017","article-title":"Identification and expression of complement component C8\u03b1, C8\u03b2 and C8\u03b3 gene in half-smooth tongue sole (Cynoglossus semilaevis) and C8\u03b1 recombinant protein antibacterial activity analysis","volume":"72","author":"Yang","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib22","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.fsi.2014.11.018","article-title":"Transcriptome analysis revealed changes of multiple genes involved in immunity in Cynoglossus semilaevis during Vibrio anguillarum infection","volume":"43","author":"Zhang","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib23","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1111\/j.1095-8649.2010.02592.x","article-title":"Generation and analysis of 10 000 ESTs from the half-smooth tongue sole Cynoglossus semilaevis and identification of microsatellite and SNP markers","volume":"76","author":"Sha","year":"2010","journal-title":"J. Fish. Biol."},{"key":"10.1016\/j.fsi.2020.05.042_bib24","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.fsi.2020.05.042_bib25","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1016\/j.fsi.2012.01.010","article-title":"Identification and expression analysis of goose-type lysozyme in half-smooth tongue sole (Cynoglossus semilaevis)","volume":"32","author":"Sha","year":"2012","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib26","doi-asserted-by":"crossref","unstructured":"T.D. Schmittgen, K.J. Livak, Analyzing real-time PCR data by the comparative CT method, 3 (n.d.) 1101\u20131108.","DOI":"10.1038\/nprot.2008.73"},{"key":"10.1016\/j.fsi.2020.05.042_bib27","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.fsi.2017.04.015","article-title":"Isolation and characterization of monocyte\/macrophage from peripheral blood of half smooth tongue sole (Cynoglossus semilaevis)","volume":"65","author":"Sha","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib28","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.fsi.2006.02.008","article-title":"Expression and phylogenetic analysis of the ninth complement component (C9) in rainbow trout","volume":"21","author":"Chondrou","year":"2006","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib29","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1111\/j.0105-2896.2004.00118.x","article-title":"Primitive complement system of invertebrates","volume":"198","author":"Nonaka","year":"2010","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.fsi.2020.05.042_bib30","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1007\/s00239-001-0068-z","article-title":"C6-Like and C3-like molecules from the cephalochordate, amphioxus, suggest a cytolytic complement system in invertebrates","volume":"54","author":"Suzuki","year":"2002","journal-title":"J. Mol. Evol."},{"key":"10.1016\/j.fsi.2020.05.042_bib31","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1007\/PL00006550","article-title":"Phylogenetic analysis of the homologous proteins of the terminal complement complex supports the emergence of C6 and C7 followed by C8 and C9","volume":"49","author":"Mondrag\u00f3n-Palomino","year":"1999","journal-title":"J. Mol. Evol."},{"key":"10.1016\/j.fsi.2020.05.042_bib32","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.dci.2012.09.005","article-title":"Genomic characterization and expression analysis of complement component 8\u03b1 and 8\u03b2 in rock bream (Oplegnathus fasciatus)","volume":"39","author":"Wickramaarachchi","year":"2013","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib33","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1042\/bj2440393","article-title":"Bacterial killing by complement. C9-mediated killing in the absence of C5b-8","volume":"244","author":"Dankert","year":"1987","journal-title":"Biochem. J."},{"key":"10.1016\/j.fsi.2020.05.042_bib34","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1146\/annurev.biochem.66.1.823","article-title":"The molecular structure of cell adhesion molecules","volume":"66","author":"Chothia","year":"1997","journal-title":"Annu. Rev. Biochem."},{"key":"10.1016\/j.fsi.2020.05.042_bib35","doi-asserted-by":"crossref","first-page":"2563","DOI":"10.1016\/j.bbamcr.2014.05.014","article-title":"Cytokines and chemokines: at the crossroads of cell signalling and inflammatory disease","volume":"1843","author":"Turner","year":"2014","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"10.1016\/j.fsi.2020.05.042_bib36","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.vetimm.2009.09.021","article-title":"Toll-like receptor signaling in bony fish","volume":"134","author":"Rebl","year":"2010","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.fsi.2020.05.042_bib37","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.humimm.2019.01.001","article-title":"CD3+CD20+ T cells and their roles in human diseases","volume":"80","author":"Chen","year":"2019","journal-title":"Hum. Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib38","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.imlet.2018.01.009","article-title":"CD45 in human physiology and clinical medicine","volume":"196","author":"Rheinl\u00e4nder","year":"2018","journal-title":"Immunol. Lett."},{"key":"10.1016\/j.fsi.2020.05.042_bib39","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0092-8674(85)80105-7","article-title":"The isolation and nucleotide sequence of a cDNA encoding the T cell surface protein T4: a new member of the immunoglobulin gene family","volume":"42","author":"Maddon","year":"1985","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2020.05.042_bib40","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1084\/jem.174.3.561","article-title":"CTLA-4 is a second receptor for the B cell activation antigen B7","volume":"174","author":"Linsley","year":"1991","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.fsi.2020.05.042_bib41","doi-asserted-by":"crossref","first-page":"5379","DOI":"10.4049\/jimmunol.175.8.5379","article-title":"A critical role for complement C3d and the B cell coreceptor (CD19\/CD21) complex in the initiation of inflammatory arthritis","volume":"175","author":"Del Nagro","year":"2005","journal-title":"J. Immunol."},{"key":"10.1016\/j.fsi.2020.05.042_bib42","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1016\/S1074-7613(00)80124-7","article-title":"CD72-deficient mice reveal nonredundant roles of CD72 in B cell development and activation","volume":"11","author":"Pan","year":"1999","journal-title":"Immunity"}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464820303685?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464820303685?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T05:21:20Z","timestamp":1760937680000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464820303685"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9]]},"references-count":42,"alternative-id":["S1050464820303685"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2020.05.042","relation":{},"ISSN":["1050-4648"],"issn-type":[{"type":"print","value":"1050-4648"}],"subject":[],"published":{"date-parts":[[2020,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Cloning, expression profile of the complement component C9 gene and influence of the recombinant C9 protein on peripheral mononuclear leukocytes transcriptome in half-smooth tongue sole (Cynoglossus semilaevis)","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2020.05.042","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}