{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T17:32:12Z","timestamp":1779125532292,"version":"3.51.4"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100010029","name":"Taishan Scholar Foundation of Shandong Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010029","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2021,8]]},"DOI":"10.1016\/j.fsi.2021.05.018","type":"journal-article","created":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T13:22:00Z","timestamp":1622121720000},"page":"27-34","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"special_numbering":"C","title":["Characterization of toll-like receptor 1 (TLR1) in turbot (Scophthalmus maximus L.)"],"prefix":"10.1016","volume":"115","author":[{"given":"Junxia","family":"Yu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beibei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baofeng","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fenghua","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1950-0190","authenticated-orcid":false,"given":"Chao","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2021.05.018_bib1","doi-asserted-by":"crossref","first-page":"486","DOI":"10.17221\/3294-VETMED","article-title":"Innate and adaptive immunity in teleost fish: a review","volume":"56","author":"Uribe","year":"2011","journal-title":"Vet. Med."},{"key":"10.1016\/j.fsi.2021.05.018_bib2","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/j.cell.2010.01.022","article-title":"Pattern recognition receptors and inflammation","volume":"140","author":"Takeuchi","year":"2010","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2021.05.018_bib3","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.1016\/j.dci.2011.02.010","article-title":"PAMPs, PRRs and the genomics of gram negative bacterial recognition in fish","volume":"35","author":"BoltaA","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib4","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.imlet.2013.11.013","article-title":"Toll-like receptors (TLRs) in aquatic animals: signaling pathways, expressions and immune responses","volume":"158","author":"Rauta","year":"2014","journal-title":"Immunol. Lett."},{"key":"10.1016\/j.fsi.2021.05.018_bib5","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1146\/annurev.immunol.21.120601.141126","article-title":"Toll-like receptors","volume":"21","author":"Takeda","year":"2003","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib6","doi-asserted-by":"crossref","first-page":"10","DOI":"10.4049\/jimmunol.169.1.10","article-title":"Cutting Edge: role of Toll-like receptor 1 in mediating immune response to microbial lipoproteins","volume":"169","author":"Takeuchi","year":"2002","journal-title":"J. Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib7","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1016\/j.cell.2007.09.008","article-title":"Crystal structure of the TLR1-TLR2 heterodimer induced by binding of a tri-acylated lipopeptide","volume":"130","author":"Jin","year":"2007","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2021.05.018_bib8","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.vetimm.2011.02.016","article-title":"Cloning and expression of Toll-like receptors 1 and 2 from a teleost fish, the orange-spotted grouper Epinephelus coioides","volume":"141","author":"Wei","year":"2011","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.fsi.2021.05.018_bib9","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.dci.2018.04.022","article-title":"Genomic structure and molecular characterization of Toll-like receptors 1 and 2 from golden pompano Trachinotus ovatus (Linnaeus, 1758) and their expression response to three types of pathogen-associated molecular patterns","volume":"86","author":"Wu","year":"2018","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib10","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1111\/j.1744-313X.2008.00759.x","article-title":"Characterization, expression and evolution analysis of Toll-like receptor 1 gene in pufferfish (Tetraodon nigroviridis)","volume":"35","author":"Wu","year":"2008","journal-title":"Int. J. Immunogenet."},{"key":"10.1016\/j.fsi.2021.05.018_bib11","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.1111\/jfb.12997","article-title":"Characterization, expression analysis and localization pattern of toll\u2010like receptor 1 (tlr1) and toll\u2010like receptor 2 (tlr2) genes in grass carp Ctenopharyngodon idella","volume":"89","author":"He","year":"2016","journal-title":"J. Fish. Biol."},{"key":"10.1016\/j.fsi.2021.05.018_bib12","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.dci.2017.03.021","article-title":"Cloning and expression analysis of a Toll-like receptor 21 (TLR21) gene from turbot, Scophthalmus maximus","volume":"73","author":"Li","year":"2017","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib13","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1111\/jfb.12559","article-title":"A toll-like receptor 3 homologue that is up-regulated by poly I:C and DNA virus in turbot Scophthalmus maximus","volume":"86","author":"Hu","year":"2015","journal-title":"J. Fish. Biol."},{"issue":"2","key":"10.1016\/j.fsi.2021.05.018_bib14","doi-asserted-by":"crossref","DOI":"10.32629\/jcmr.v1i2.188","article-title":"Molecular characterization and expression analysis of toll-like receptor 9 gene in turbot, Scophthalmus maximus","volume":"1","author":"Wang","year":"2020","journal-title":"J. Clin. Med. Res."},{"key":"10.1016\/j.fsi.2021.05.018_bib15","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.dci.2018.03.020","article-title":"Molecular cloning and expression study on Toll-like receptor 5M in turbot, Scophthalmus maximus","volume":"85","author":"Liu","year":"2018","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib16","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.fsi.2017.05.025","article-title":"Characterization of Toll-like receptor 22 in turbot (Scophthalmus maximus)","volume":"66","author":"Jing","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib17","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.fsi.2016.09.021","article-title":"Identification and expression analysis of toll-like receptor genes (TLR8 and TLR9) in mucosal tissues of turbot (Scophthalmus maximus L.) following bacterial challenge","volume":"58","author":"Dong","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib18","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1007\/s00018-005-5035-1","article-title":"A marvel of mucosal T cells and secretory antibodies for the creation of first lines of defense","volume":"62","author":"Kunisawa","year":"2005","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.fsi.2021.05.018_bib19","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.ijdevneu.2015.06.004","article-title":"Neuroanatomical localization of nitric oxide synthase (nNOS) in the central nervous system of carp, Labeo rohita during post-embryonic development","volume":"46","author":"Biswas","year":"2015","journal-title":"Int. J. Dev. Neurosci."},{"key":"10.1016\/j.fsi.2021.05.018_bib20","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.dci.2016.07.007","article-title":"Transcriptomic profiling revealed the signatures of intestinal barrier alteration and pathogen entry in turbot (Scophthalmus maximus) following Vibrio anguillarum challenge","volume":"65","author":"Gao","year":"2016","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib21","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1093\/dnares\/dsw007","article-title":"Whole genome sequencing of turbot (Scophthalmus maximus; Pleuronectiformes): a fish adapted to demersal life","volume":"23","author":"Figueras","year":"2016","journal-title":"DNA Res."},{"key":"10.1016\/j.fsi.2021.05.018_bib22","first-page":"571","article-title":"Protein identification and analysis tools in the ExPASy server","volume":"112","author":"Wilkins","year":"1999","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.fsi.2021.05.018_bib23","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: molecular evolutionary Genetics analysis version 6.0","volume":"30","author":"Tamura","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.fsi.2021.05.018_bib24","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/msb.2011.75","article-title":"Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega","volume":"7","author":"Sievers","year":"2011","journal-title":"Mol. Syst. Biol."},{"key":"10.1016\/j.fsi.2021.05.018_bib25","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0145-305X(82)80011-6","article-title":"Rainbow trout macrophages in vitro: morphology and phagocytic activity","volume":"6","author":"Braun-Nesje","year":"1982","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib26","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1016\/0092-8674(85)90275-2","article-title":"Establishment of dorsal-ventral polarity in the drosophila embryo: the induction of polarity by the Toll gene product","volume":"42","author":"Anderson","year":"1985","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2021.05.018_bib27","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1126\/science.282.5396.2085","article-title":"Defective LPS signaling in C3H\/HeJ and C57BL\/10ScCr mice: mutations in Tlr4 gene","volume":"282","author":"Poltorak","year":"1999","journal-title":"Science"},{"key":"10.1016\/j.fsi.2021.05.018_bib28","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.fsi.2016.06.047","article-title":"Identification and expression analysis of TLR2 in mucosal tissues of turbot (Scophthalmus maximus L.) following bacterial challenge","volume":"55","author":"Liu","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib29","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.dci.2013.01.009","article-title":"Pathogen recognition receptors in channel catfish: III Phylogeny and expression analysis of Toll-like receptors","volume":"40","author":"Zhang","year":"2013","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib30","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1016\/j.fsi.2010.02.002","article-title":"Identification, characterization and genetic mapping of TLR1 loci in rainbow trout (Oncorhynchus mykiss)","volume":"28","author":"Palti","year":"2010","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib31","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1084\/jem.20112339","article-title":"A specific role for TLR1 in protective T(H)17 immunity during mucosal infection","volume":"209","author":"DePaolo","year":"2012","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.fsi.2021.05.018_bib32","doi-asserted-by":"crossref","first-page":"230","DOI":"10.4049\/jimmunol.1701216","article-title":"Innate recognition of the microbiota by TLR1 promotes epithelial homeostasis and prevents chronic inflammation","volume":"201","author":"Kamdar","year":"2018","journal-title":"J. Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib33","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.fsi.2016.07.024","article-title":"Comparative genomic evidence for duplication of TLR1 subfamily and miiuy croaker TLR1 perceives LPS stimulation via MyD88 and TIRAP","volume":"56","author":"Xu","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib34","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1111\/j.1365-2761.1996.tb00129.x","article-title":"Is the turbot, Scophthalmus maximus (L.), intestine a portal of entry for the fish pathogen Vibrio anguillarum?","volume":"19","author":"Oisson","year":"2006","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2021.05.018_bib35","doi-asserted-by":"crossref","first-page":"7125","DOI":"10.4049\/jimmunol.165.12.7125","article-title":"Evidence for an accessory protein function for toll-like receptor 1 in anti-bacterial responses","volume":"165","author":"Wyllie","year":"2001","journal-title":"J. Immunol."},{"key":"10.1016\/j.fsi.2021.05.018_bib36","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1016\/j.ijbiomac.2020.07.293","article-title":"Structure of fish Toll-like receptors (TLR) and NOD-like receptors (NLR)","volume":"161","author":"Sahoo","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.fsi.2021.05.018_bib37","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1016\/j.ijbiomac.2020.07.111","article-title":"Genome-wide characterization of Toll-like receptors in black rockfish Sebastes schlegelii: evolution and response mechanisms following Edwardsiella tarda infection","volume":"164","author":"Cao","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.fsi.2021.05.018_bib38","doi-asserted-by":"crossref","first-page":"2563","DOI":"10.1016\/j.bbamcr.2014.05.014","article-title":"Cytokines and chemokines: at the crossroads of cell signalling and inflammatory disease","volume":"1843","author":"Turner","year":"2014","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"10.1016\/j.fsi.2021.05.018_bib39","doi-asserted-by":"crossref","first-page":"2913","DOI":"10.4049\/jimmunol.173.5.2913","article-title":"Cutting edge: TNFR-associated factor (TRAF) 6 is essential for MyD88-dependent pathway but not toll\/IL-1 receptor domain-containing adaptor-inducing IFN-beta (TRIF)-dependent pathway in TLR signaling","volume":"173","author":"Gohda","year":"2014","journal-title":"J. Immunol."}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464821001467?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464821001467?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T05:26:34Z","timestamp":1760937994000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464821001467"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8]]},"references-count":39,"alternative-id":["S1050464821001467"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2021.05.018","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2021,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization of toll-like receptor 1 (TLR1) in turbot (Scophthalmus maximus L.)","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2021.05.018","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2021 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}