{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T20:07:08Z","timestamp":1782158828812,"version":"3.54.5"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2022,6]]},"DOI":"10.1016\/j.fsi.2022.05.007","type":"journal-article","created":{"date-parts":[[2022,5,7]],"date-time":"2022-05-07T11:23:07Z","timestamp":1651922587000},"page":"84-89","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Prediction and characterization of a novel hemoglobin-derived mutant peptide (mTgHbP7) from Tegillarca granosa"],"prefix":"10.1016","volume":"125","author":[{"given":"Xiaoying","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8840-467X","authenticated-orcid":false,"given":"Shanjie","family":"Zha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8268-1887","authenticated-orcid":false,"given":"Yongbo","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.fsi.2022.05.007_bib1","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/S0968-0896(00)00263-7","article-title":"Broad-spectrum antimicrobial activity of hemoglobin","volume":"9","author":"Parish","year":"2001","journal-title":"Bioorg. Med. Chem."},{"issue":"8","key":"10.1016\/j.fsi.2022.05.007_bib2","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1007\/s00726-018-2575-x","article-title":"Prediction and characterization of a novel hemocyanin-derived antimicrobial peptide from shrimp Litopenaeus vannamei","volume":"50","author":"Yang","year":"2018","journal-title":"Amino Acids"},{"key":"10.1016\/j.fsi.2022.05.007_bib3","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.rinim.2012.03.001","article-title":"Rondonin an antifungal peptide from spider (Acanthoscurria rondoniae) haemolymph","volume":"2","author":"Riciluca","year":"2012","journal-title":"Results.Immunol."},{"key":"10.1016\/j.fsi.2022.05.007_bib4","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.toxicon.2013.03.011","article-title":"Melittin peptide kills Trypanosoma cruzi parasites by inducing different cell death pathways","volume":"69","author":"Adade","year":"2013","journal-title":"Toxicon"},{"issue":"9","key":"10.1016\/j.fsi.2022.05.007_bib5","doi-asserted-by":"crossref","first-page":"2180","DOI":"10.1016\/j.bbamem.2014.04.015","article-title":"Peptide entry inhibitors of enveloped viruses: the importance of interfacial hydrophobicity","volume":"1838","author":"Badani","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"10.1016\/j.fsi.2022.05.007_bib6","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.biochi.2014.06.014","article-title":"Antifungal activity and pore-forming mechanism of astacidin 1 against Candida albicans","volume":"105","author":"Choi","year":"2014","journal-title":"Biochimie"},{"issue":"1","key":"10.1016\/j.fsi.2022.05.007_bib7","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.actbio.2013.08.043","article-title":"Design of imperfectly amphipathic alpha-helical antimicrobial peptides with enhanced cell selectivity","volume":"10","author":"Zhu","year":"2014","journal-title":"Acta Biomater."},{"issue":"1","key":"10.1016\/j.fsi.2022.05.007_bib8","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.dci.2014.11.008","article-title":"Identification of candidate antimicrobial peptides derived from abalone hemocyanin","volume":"49","author":"Zhuang","year":"2015","journal-title":"Dev. Comp. Immunol."},{"issue":"3","key":"10.1016\/j.fsi.2022.05.007_bib9","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1111\/cbdd.12667","article-title":"Design, synthesis, and biological evaluation of novel cholesteryl peptides with anticancer and multidrug resistance-reversing activities","volume":"87","author":"Deng","year":"2016","journal-title":"Chem. Biol. Drug Des."},{"issue":"6","key":"10.1016\/j.fsi.2022.05.007_bib10","doi-asserted-by":"crossref","first-page":"1696","DOI":"10.1016\/j.fsi.2013.03.180","article-title":"Hemoglobin and its derived peptides from blood clam (Tegillarca granosa) exhibiting an antimicrobial activity","volume":"34","author":"Bao","year":"2013","journal-title":"Fish Shellfish Immunol."},{"issue":"4","key":"10.1016\/j.fsi.2022.05.007_bib11","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.fsi.2011.05.029","article-title":"Hemoglobin of the bloody clam Tegillarca granosa (Tg-HbI) is involved in the immune response against bacterial infection","volume":"31","author":"Bao","year":"2011","journal-title":"Fish Shellfish Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2022.05.007_bib12","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1093\/humrep\/den318","article-title":"Hemoglobin and its derived peptides may play a role in the antibacterial mechanism of the vagina","volume":"24","author":"Deng","year":"2009","journal-title":"Hum. Reprod."},{"issue":"11","key":"10.1016\/j.fsi.2022.05.007_bib13","doi-asserted-by":"crossref","first-page":"1839","DOI":"10.1016\/j.peptides.2004.06.015","article-title":"Antibacterial hemoglobin peptides in human menstrual blood","volume":"25","author":"Mak","year":"2004","journal-title":"Peptides"},{"issue":"3","key":"10.1016\/j.fsi.2022.05.007_bib14","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.fsi.2012.08.022","article-title":"Isolation and characterization of a novel antibacterial peptide derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica","volume":"35","author":"Zhang","year":"2013","journal-title":"Fish Shellfish Immunol."},{"issue":"32","key":"10.1016\/j.fsi.2022.05.007_bib15","doi-asserted-by":"crossref","first-page":"12011","DOI":"10.1073\/pnas.0601861103","article-title":"Red blood with blue-blood ancestry: intriguing structure of a snail hemoglobin","volume":"103","author":"Lieb","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"9","key":"10.1016\/j.fsi.2022.05.007_bib16","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0184584","article-title":"Transcriptome analysis of sex-related genes in the blood clam Tegillarca granosa","volume":"12","author":"Chen","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2022.05.007_bib17","first-page":"149","article-title":"Hemoglobin from the blood clam Tegillarca granosa (Tg-HbIIA, Tg-HbIIB): expression and antibacterial activity of recombinant proteins","volume":"14","author":"Song","year":"2017","journal-title":"Invertebr. Surviv. J."},{"key":"10.1016\/j.fsi.2022.05.007_bib18","first-page":"106","article-title":"Antibacterial activity and mechanisms of heterotetramer hemoglobin derived polypeptide from Tegillarca granosa","volume":"37","author":"Wang J","year":"2015","journal-title":"Hai Yang Xue Bao"},{"key":"10.1016\/j.fsi.2022.05.007_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2020.104302","article-title":"Antimicrobial activity and acting mechanism of Tegillarca granosa hemoglobin-derived peptide (TGH1) against Vibrio parahaemolyticus","author":"Yang","year":"2020","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.fsi.2022.05.007_bib20","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.fsi.2016.02.004","article-title":"A preliminary study on the antibacterial mechanism of Tegillarca granosa hemoglobin by derived peptides and peroxidase activity","volume":"51","author":"Bao","year":"2016","journal-title":"Fish Shellfish Immunol."},{"issue":"13","key":"10.1016\/j.fsi.2022.05.007_bib21","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.1093\/bioinformatics\/btx081","article-title":"Empirical comparison of web-based antimicrobial peptide prediction tools","volume":"33","author":"Gabere","year":"2017","journal-title":"Bioinformatics"},{"issue":"3","key":"10.1016\/j.fsi.2022.05.007_bib22","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/S1473-3099(01)00092-5","article-title":"Cationic peptides: effectors in innate immunity and novel antimicrobials","volume":"1","author":"Hancock","year":"2001","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.fsi.2022.05.007_bib23","doi-asserted-by":"crossref","first-page":"9388","DOI":"10.1038\/srep09388","article-title":"Hydrophobic mismatch demonstrated for membranolytic peptides, and their use as molecular rulers to measure bilayer thickness in native cells","volume":"5","author":"Grau-Campistany","year":"2015","journal-title":"Sci. Rep."},{"issue":"6","key":"10.1016\/j.fsi.2022.05.007_bib24","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1089\/mdr.2017.0392","article-title":"Antimicrobial peptides: features, action, and their resistance mechanisms in bacteria","volume":"24","author":"Moravej","year":"2018","journal-title":"Microb. Drug Resist."},{"issue":"1\u20132","key":"10.1016\/j.fsi.2022.05.007_bib25","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/S0005-2736(99)00201-1","article-title":"Structural features of helical antimicrobial peptides: their potential to modulate activity on model membranes and biological cells","volume":"1462","author":"Dathe","year":"1999","journal-title":"Biochim. Biophys. Acta Biomembr."},{"issue":"4","key":"10.1016\/j.fsi.2022.05.007_bib26","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1016\/j.peptides.2005.08.016","article-title":"Design and synthesis of novel antimicrobial peptides on the basis of alpha helical domain of Tenecin 1, an insect defensin protein, and structure-activity relationship study","volume":"27","author":"Ahn","year":"2006","journal-title":"Peptides"},{"issue":"38","key":"10.1016\/j.fsi.2022.05.007_bib27","doi-asserted-by":"crossref","first-page":"4270","DOI":"10.2174\/138161211798999447","article-title":"Plant defensins and defensin-like peptides - biological activities and biotechnological applications","volume":"17","author":"Carvalho Ade","year":"2011","journal-title":"Curr. Pharmaceut. Des."},{"issue":"1","key":"10.1016\/j.fsi.2022.05.007_bib28","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1111\/cbdd.13058","article-title":"Role of positively charged residues on the polar and non-polar faces of amphipathic alpha-helical antimicrobial peptides on specificity and selectivity for Gram-negative pathogens","volume":"91","author":"Jiang","year":"2018","journal-title":"Chem. Biol. Drug Des."},{"issue":"6","key":"10.1016\/j.fsi.2022.05.007_bib29","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1111\/j.1747-0285.2008.00728.x","article-title":"Effects of hydrophobicity on the antifungal activity of alpha-helical antimicrobial peptides","volume":"72","author":"Jiang","year":"2008","journal-title":"Chem. Biol. Drug Des."},{"key":"10.1016\/j.fsi.2022.05.007_bib30","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/j.colsurfb.2016.02.003","article-title":"Role of amphipathicity and hydrophobicity in the balance between hemolysis and peptide-membrane interactions of three related antimicrobial peptides","volume":"141","author":"Hollmann","year":"2016","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"10.1016\/j.fsi.2022.05.007_bib31","series-title":"Application of Tryptophan to Enhance Bactericidal Activity of Gram-Negative Bacteria","author":"Zhang","year":"2020"},{"issue":"3","key":"10.1016\/j.fsi.2022.05.007_bib32","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1007\/s10989-019-09946-9","article-title":"Antimicrobial peptides (AMPs): roles, functions and mechanism of action","volume":"26","author":"Seyfi","year":"2019","journal-title":"Int. J. Pept. Res. Therapeut."},{"issue":"D1","key":"10.1016\/j.fsi.2022.05.007_bib33","doi-asserted-by":"crossref","first-page":"D1087","DOI":"10.1093\/nar\/gkv1278","article-title":"APD3: the antimicrobial peptide database as a tool for research and education","volume":"44","author":"Wang","year":"2016","journal-title":"Nucleic Acids Res."},{"issue":"Database issue","key":"10.1016\/j.fsi.2022.05.007_bib34","doi-asserted-by":"crossref","first-page":"D586","DOI":"10.1093\/nar\/gkh032","article-title":"ANTIMIC: a database of antimicrobial sequences","volume":"32","author":"Brahmachary","year":"2004","journal-title":"Nucleic Acids Res."},{"issue":"D1","key":"10.1016\/j.fsi.2022.05.007_bib35","doi-asserted-by":"crossref","first-page":"D488","DOI":"10.1093\/nar\/gkab651","article-title":"Dramp 3.0: an enhanced comprehensive data repository of antimicrobial peptides","volume":"50","author":"Shi","year":"2022","journal-title":"Nucleic Acids Res."},{"issue":"6","key":"10.1016\/j.fsi.2022.05.007_bib36","doi-asserted-by":"crossref","first-page":"2649","DOI":"10.1007\/s00253-014-6077-9","article-title":"Design and recombination expression of a novel plectasin-derived peptide MP1106 and its properties against Staphylococcus aureus","volume":"99","author":"Cao","year":"2015","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.fsi.2022.05.007_bib37","first-page":"342","article-title":"Research progress on molecular structure and functional characteristics of natural antimicrobial peptides","volume":"19","author":"Meng","year":"2019","journal-title":"J. Chin. Inst. Food Sci. Technol."},{"issue":"1","key":"10.1016\/j.fsi.2022.05.007_bib38","first-page":"98","article-title":"Structure reformation strategy of natural antimicrobial peptides","volume":"25","author":"Liu","year":"2015","journal-title":"Biotechnology"},{"key":"10.1016\/j.fsi.2022.05.007_bib39","first-page":"66","article-title":"Design of peptide with higher activity by studying the Granulysin's antibacterial mechanism","volume":"3","author":"Chen","year":"2005","journal-title":"Chin. J. Bioprocess Eng."},{"issue":"11","key":"10.1016\/j.fsi.2022.05.007_bib40","doi-asserted-by":"crossref","first-page":"4180","DOI":"10.1529\/biophysj.106.084293","article-title":"TASSER-Lite: an automated tool for protein comparative modeling","volume":"91","author":"Pandit","year":"2006","journal-title":"Biophys. J."},{"issue":"1","key":"10.1016\/j.fsi.2022.05.007_bib41","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1038\/nmeth.3213","article-title":"The I-TASSER Suite: protein structure and function prediction","volume":"12","author":"Yang","year":"2015","journal-title":"Nat. Methods"},{"issue":"7","key":"10.1016\/j.fsi.2022.05.007_bib42","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.tips.2019.04.012","article-title":"The vast structural diversity of antimicrobial peptides","volume":"40","author":"Koehbach","year":"2019","journal-title":"Trends Pharmacol. Sci."},{"issue":"1","key":"10.1016\/j.fsi.2022.05.007_bib43","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1124\/pr.55.1.2","article-title":"Mechanisms of antimicrobial peptide action and resistance","volume":"55","author":"Yeaman","year":"2003","journal-title":"Pharmacol. Rev."},{"key":"10.1016\/j.fsi.2022.05.007_bib44","doi-asserted-by":"crossref","first-page":"867381","DOI":"10.1155\/2014\/867381","article-title":"Antimicrobial peptides: their role as infection-selective tracers for molecular imaging","volume":"2014","author":"Ebenhan","year":"2014","journal-title":"BioMed Res. Int."},{"issue":"9","key":"10.1016\/j.fsi.2022.05.007_bib45","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.tibtech.2011.05.001","article-title":"The expanding scope of antimicrobial peptide structures and their modes of action","volume":"29","author":"Nguyen","year":"2011","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.fsi.2022.05.007_bib46","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.jcis.2018.10.103","article-title":"Membrane targeting cationic antimicrobial peptides","volume":"537","author":"Ciumac","year":"2019","journal-title":"J. Colloid Interface Sci."},{"issue":"8","key":"10.1016\/j.fsi.2022.05.007_bib47","doi-asserted-by":"crossref","DOI":"10.3390\/md19080420","article-title":"Large-scale plasma peptidomic profiling reveals a novel, nontoxic, Crassostrea hongkongensis-derived antimicrobial peptide against foodborne pathogens","volume":"19","author":"Mao","year":"2021","journal-title":"Mar. Drugs"},{"issue":"3","key":"10.1016\/j.fsi.2022.05.007_bib48","doi-asserted-by":"crossref","first-page":"529","DOI":"10.3934\/microbiol.2017.3.529","article-title":"Foodborne pathogens","volume":"3","author":"Bintsis","year":"2017","journal-title":"AIMS Microbiology"},{"key":"10.1016\/j.fsi.2022.05.007_bib49","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/978-1-4939-2074-7_26","article-title":"General cytotoxicity assessment by means of the MTT assay","volume":"1250","author":"Tolosa","year":"2015","journal-title":"Methods Mol. Biol."},{"issue":"7","key":"10.1016\/j.fsi.2022.05.007_bib50","first-page":"3919","article-title":"The antimicrobial peptides and their potential clinical applications","volume":"11","author":"Lei","year":"2019","journal-title":"Am. J. Tourism Res."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464822002418?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464822002418?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T05:32:56Z","timestamp":1760938376000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464822002418"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6]]},"references-count":50,"alternative-id":["S1050464822002418"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2022.05.007","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2022,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Prediction and characterization of a novel hemoglobin-derived mutant peptide (mTgHbP7) from Tegillarca granosa","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2022.05.007","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}