{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T05:09:15Z","timestamp":1774933755683,"version":"3.50.1"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2022,7]]},"DOI":"10.1016\/j.fsi.2022.05.050","type":"journal-article","created":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T02:24:33Z","timestamp":1653618273000},"page":"311-317","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["Response of cecropin transgenesis to challenge with Edwardsiella ictaluri in channel catfish Ictalurus punctatus"],"prefix":"10.1016","volume":"126","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3590-2269","authenticated-orcid":false,"given":"Nermeen Y.","family":"Abass","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rhoda Mae C.","family":"Simora","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinhai","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shangjia","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"De","family":"Xing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Coogan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Creamer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rex A.","family":"Dunham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2022.05.050_bib1","series-title":"The State of World Fisheries and Aquaculture 2020","year":"2020"},{"key":"10.1016\/j.fsi.2022.05.050_bib2","series-title":"National Agricultural Statistics Service (NASS), Agricultural Statistics Board, Catfish Production","year":"2019"},{"key":"10.1016\/j.fsi.2022.05.050_bib3","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/0959-8030(93)90028-A","article-title":"Pathogenesis of gram-negative bacterial infections in warmwater fish","volume":"3","author":"Thune","year":"1993","journal-title":"Annu. Rev. Fish Dis."},{"key":"10.1016\/j.fsi.2022.05.050_bib4","series-title":"Parasites of North American Freshwater FIshes","author":"Hoffman","year":"1999"},{"key":"10.1016\/j.fsi.2022.05.050_bib5","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1577\/1548-8667(1998)010<0143:IMIEIS>2.0.CO;2","article-title":"Ichthyophthirius multifiliis (Ich) epizootics in spawning sockeye salmon in British Columbia, Canada","volume":"10","author":"Traxler","year":"1998","journal-title":"J. Aquat. Anim. Health"},{"key":"10.1016\/j.fsi.2022.05.050_bib6","doi-asserted-by":"crossref","first-page":"1508","DOI":"10.1139\/f79-219","article-title":"A bacterium associated with disease of pond cultured channel catfish, Ictalurus punctatus","volume":"36","author":"Hawke","year":"1979","journal-title":"J. Fish. Res. Board Can."},{"key":"10.1016\/j.fsi.2022.05.050_bib7","first-page":"396","article-title":"Edwardsiella ictaluri sp. nov., the causative agent of enteric septicemia of catfish","volume":"31","author":"Hawke","year":"1981","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"10.1016\/j.fsi.2022.05.050_bib8","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1111\/j.1749-7345.2009.00279.x","article-title":"Use of modified live vaccines in aquaculture","volume":"40","author":"Shoemaker","year":"2009","journal-title":"J. World Aquacult. Soc."},{"key":"10.1016\/j.fsi.2022.05.050_bib9","series-title":"Enteric Septicemia of Catfish","author":"Hawke","year":"2015"},{"key":"10.1016\/j.fsi.2022.05.050_bib10","series-title":"Health Maintenance and Principal Microbial Diseases of Cultured Fishes","first-page":"328","author":"Plumb","year":"1999"},{"key":"10.1016\/j.fsi.2022.05.050_bib11","first-page":"139","article-title":"Transgenic fish resistant to infectious diseases, their risk and prevention of escape into the environment and future candidate genes for disease transgene manipulation, Comparative Immunology","volume":"32","author":"Dunham","year":"2009","journal-title":"Microbiol. Infect. Dis."},{"key":"10.1016\/j.fsi.2022.05.050_bib12","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1111\/raq.12205","article-title":"Disease reduction in aquaculture with genetic and genomic technology: current and future approaches","volume":"10","author":"Elaswad","year":"2018","journal-title":"Rev. Aquacult."},{"key":"10.1016\/j.fsi.2022.05.050_bib13","series-title":"Aquaculture and Fisheries Biotechnology: Genetic Approaches","first-page":"495","author":"Dunham","year":"2011"},{"key":"10.1016\/j.fsi.2022.05.050_bib14","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/j.1432-1033.1980.tb05991.x","article-title":"Insect immunity. purification and properties of three inducible bactericidal proteins from hemolymph of immunized pupae of Hyalophora cecropia","volume":"106","author":"Hultmark","year":"1980","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.fsi.2022.05.050_bib15","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.1093\/jac\/37.6.1077","article-title":"Antimicrobial activity of cecropins","volume":"37","author":"Moore","year":"1996","journal-title":"J. Antimicrob. Chemother."},{"key":"10.1016\/j.fsi.2022.05.050_bib16","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1007\/s002329900201","article-title":"Structure and functions of channel-forming peptides: magainins, cecropins, melittin and alamethicin","volume":"156","author":"Bechinger","year":"1997","journal-title":"J. Membr. Biol."},{"key":"10.1016\/j.fsi.2022.05.050_bib17","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1111\/j.1399-3011.1992.tb00321.x","article-title":"Antibacterial peptides designed as analogs or hybrids of cecropins and melittin","volume":"40","author":"Wade","year":"2009","journal-title":"Int. J. Pept. Protein Res."},{"key":"10.1016\/j.fsi.2022.05.050_bib18","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1111\/j.1399-3011.1997.tb01121.x","article-title":"Synthesis and antibacterial action of cecropin and proline-arginine-rich peptides from pig intestine","volume":"49","author":"Vunnam","year":"2009","journal-title":"J. Pept. Res."},{"key":"10.1016\/j.fsi.2022.05.050_bib19","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S0304-4165(97)00024-X","article-title":"Effects of the anti-bacterial peptide cecropin B and its analogs, cecropins B-1 and B-2, on liposomes, bacteria, and cancer cells","volume":"1336","author":"Chen","year":"1997","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"10.1016\/j.fsi.2022.05.050_bib20","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1006\/jipa.1995.1047","article-title":"Effects of Synthetic Bombyx mori ceoropin B on the growth of plant pathogenic bacteria","volume":"65","author":"Kadono-Okuda","year":"1995","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.fsi.2022.05.050_bib21","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1007\/s10126-002-0024-y","article-title":"Enhanced bacterial disease resistance of transgenic channel catfish Ictalurus punctatus possessing cecropin genes","volume":"4","author":"Dunham","year":"2002","journal-title":"Mar. Biotechnol."},{"key":"10.1016\/j.fsi.2022.05.050_bib22","doi-asserted-by":"crossref","first-page":"127","DOI":"10.17660\/ActaHortic.1993.336.16","article-title":"Transformation of potato (Solanum tuberosum) with a gene for an anti-bacterial protein, cecropin","author":"Hassan","year":"1993","journal-title":"Acta Hortic."},{"key":"10.1016\/j.fsi.2022.05.050_bib23","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1002\/naaq.10096","article-title":"Effects of cecropin transgenesis and interspecific hybridization on the resistance to Ichthyophthirius multifiliis in channel catfish and female channel catfish \u00d7 male blue catfish hybrids","volume":"81","author":"Elaswad","year":"2019","journal-title":"N. Am. J. Aquacult."},{"key":"10.1016\/j.fsi.2022.05.050_bib24","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1007\/s10126-013-9550-z","article-title":"Production of homozygous transgenic rainbow trout with enhanced disease resistance","author":"Chiou","year":"2014","journal-title":"Mar. Biotechnol."},{"key":"10.1016\/j.fsi.2022.05.050_bib25","doi-asserted-by":"crossref","DOI":"10.3390\/md15060155","article-title":"Salt sensitive Tet-off-like systems to knockdown primordial germ cell genes for repressible transgenic sterilization in channel catfish, Ictalurus punctatus","volume":"15","author":"Li","year":"2017","journal-title":"Mar. Drugs"},{"key":"10.1016\/j.fsi.2022.05.050_bib26","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1007\/s10126-018-9819-3","article-title":"Repressible transgenic sterilization in channel catfish, Ictalurus punctatus, by knockdown of primordial germ cell genes with copper-sensitive constructs","volume":"20","author":"Li","year":"2018","journal-title":"Mar. Biotechnol."},{"key":"10.1016\/j.fsi.2022.05.050_bib27","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.aquaculture.2012.10.001","article-title":"Relative effectiveness of carp pituitary extract, luteininzing hormone releasing hormone analog (LHRHa) injections and LHRHa implants for producing hybrid catfish fry","volume":"372\u2013375","author":"Su","year":"2013","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2022.05.050_bib28","doi-asserted-by":"crossref","DOI":"10.1111\/jfd.13257","article-title":"Cathelicidins enhance protection of channel catfish, Ictalurus punctatus , and channel catfish \u2640 \u00d7 blue catfish, Ictalurus furcatus \u2642 hybrid catfish against Edwarsiella ictaluri infection","author":"Simora","year":"2020","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2022.05.050_bib29","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1073\/pnas.0304265101","article-title":"Transgenic zebrafish produced by retroviral infection of in vitro-cultured sperm","volume":"101","author":"Kurita","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.fsi.2022.05.050_bib30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2307\/3001478","article-title":"Multiple range and multiple F tests","volume":"11","author":"Duncan","year":"1955","journal-title":"Biometrics"},{"key":"10.1016\/j.fsi.2022.05.050_bib31","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/0044-8486(93)90025-T","article-title":"Genetic improvement of disease resistance in fish: an overview","volume":"111","author":"Fjalestad","year":"1993","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2022.05.050_bib32","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1126\/science.286.5439.420","article-title":"Defensins and host defense","volume":"286","author":"Ganz","year":"1999","journal-title":"Science"},{"key":"10.1016\/j.fsi.2022.05.050_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2021.736468","article-title":"Effects of family and promoter on growth performance of ccGH cDNA transgenic channel catfish, Ictalurus punctatus, grown in a trough culture system","volume":"536","author":"Abass","year":"2021","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2022.05.050_bib34","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1590\/S1415-47572007000100008","article-title":"Improving the production of transgenic fish germlines: in vivo evaluation of mosaicism in zebrafish (Danio rerio) using fluorescent protein (GFP) and growth hormone cDNA transgene co-injection strategy","volume":"30","author":"Figueiredo","year":"2007","journal-title":"Genet. Mol. Biol."},{"key":"10.1016\/j.fsi.2022.05.050_bib35","first-page":"e124","article-title":"Importance of transgenic fish to global aquaculture : a review","volume":"6","author":"Wakchaure","year":"2015","journal-title":"Fish. Aquacult. J."},{"key":"10.1016\/j.fsi.2022.05.050_bib36","series-title":"Transgene Insertion of Cathelicidin Gene in Channel Catfish Ictalurus punctatus Using CRISPR\/Cas9 Knock-In Technology and Cathelicidin Activity against Catfish Pathogens","author":"Simora","year":"2020"},{"key":"10.1016\/j.fsi.2022.05.050_bib37","first-page":"338","article-title":"Selection of promoters for gene transfer into fish","volume":"1","author":"Moav","year":"1992","journal-title":"Mol. Mar. Biol. Biotechnol."},{"key":"10.1016\/j.fsi.2022.05.050_bib38","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1007\/s11434-015-0893-x","article-title":"The integration characteristics of the exogenous growth hormone gene in a transgenic common carp (Cyprinus carpio L.) with fast-growth performance","volume":"60","author":"Chen","year":"2015","journal-title":"Sci. Bull."},{"key":"10.1016\/j.fsi.2022.05.050_bib39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2164-15-887","article-title":"Altered gene expression patterns of innate and adaptive immunity pathways in transgenic rainbow trout harboring cecropin P1 transgene","volume":"15","author":"Lo","year":"2014","journal-title":"BMC Genom."},{"key":"10.1016\/j.fsi.2022.05.050_bib40","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1080\/mmy.38.4.301.308","article-title":"D-cecropin b: proteolytic resistance, lethality for pathogenic fungi and binding properties","volume":"38","author":"De Lucca","year":"2000","journal-title":"Med. Mycol."},{"key":"10.1016\/j.fsi.2022.05.050_bib41","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1016\/S0965-1748(97)00049-0","article-title":"Protein purification and cDNA cloning of a cecropin\u2010like peptide from the larvae of fall webworm (Hyphantria cunea)","volume":"27","author":"Park","year":"1997","journal-title":"Insect Biochem. Mol. Biol."},{"key":"10.1016\/j.fsi.2022.05.050_bib42","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1128\/iai.57.9.2628-2633.1989","article-title":"Effects of magainins and cecropins on the sporogonic development of malaria parasites in mosquitoe","volume":"57","author":"Gwadz","year":"1989","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fsi.2022.05.050_bib43","first-page":"77","article-title":"Drosophila antibacterial protein, cecropin A, differentially affects non-bacterial organisms such as Leishmania in a manner different from other amphipathic peptides","author":"Akuffo","year":"1998","journal-title":"Int. J. Mol. Med."},{"key":"10.1016\/j.fsi.2022.05.050_bib44","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1603\/0022-2585-41.3.447","article-title":"Ectopic expression of a cecropin transgene in the human malaria vector mosquito Anopheles gambiae (Diptera: Culicidae): effects on susceptibility to Plasmodium","volume":"41","author":"Kim","year":"2004","journal-title":"J. Med. Entomol."},{"key":"10.1016\/j.fsi.2022.05.050_bib45","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1007\/s10126-002-0023-z","article-title":"Production of transgenic with increased resistance to bacterial pathogens","volume":"4","author":"Sarmasik","year":"2002","journal-title":"Mar. Biotechnol."}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464822002996?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464822002996?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T05:35:57Z","timestamp":1760938557000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464822002996"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7]]},"references-count":45,"alternative-id":["S1050464822002996"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2022.05.050","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2022,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Response of cecropin transgenesis to challenge with Edwardsiella ictaluri in channel catfish Ictalurus punctatus","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2022.05.050","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}