{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T19:27:42Z","timestamp":1793042862304,"version":"4.5.0"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2022,8]]},"DOI":"10.1016\/j.fsi.2022.06.052","type":"journal-article","created":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T12:07:32Z","timestamp":1656331652000},"page":"357-365","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"special_numbering":"C","title":["Th1\u2013Th2 and M1-M2 interplay sculpt Aeromonas hydrophila pathogenesis in zebrafish (Danio rerio)"],"prefix":"10.1016","volume":"127","author":[{"given":"Jai","family":"Kumar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manmohan","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shagun","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidhi","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashmi","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Arafat","family":"Hussain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2979-0910","authenticated-orcid":false,"given":"Shibnath","family":"Mazumder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2022.06.052_bib1","first-page":"18","article-title":"Experimental induction of motile Aeromonas septicemia in channel catfish (Ictalurus punctatus) by waterborne challenge with virulent Aeromonas hydrophila","volume":"3","author":"Zhang","year":"2016","journal-title":"Aquacult. Rep."},{"key":"10.1016\/j.fsi.2022.06.052_bib2","series-title":"Aeromonas Hydrophila and Motile Aeromonad Septicemias of Fish","first-page":"134","author":"Cipriano","year":"1984"},{"key":"10.1016\/j.fsi.2022.06.052_bib3","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.3389\/fmicb.2016.01337","article-title":"Virulence factors of Aeromonas hydrophila: in the wake of reclassification","volume":"7","author":"Rasmussen-Ivey","year":"2016","journal-title":"Front. Microbiol."},{"issue":"1","key":"10.1016\/j.fsi.2022.06.052_bib4","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1128\/CMR.00039-09","article-title":"The genus Aeromonas: taxonomy, pathogenicity, and infection","volume":"23","author":"Janda","year":"2010","journal-title":"Clin. Microbiol. Rev."},{"issue":"2","key":"10.1016\/j.fsi.2022.06.052_bib5","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1111\/jfd.12214","article-title":"Skin immune response in the zebrafish, Danio rerio (Hamilton), to Aeromonas hydrophila infection: a transcriptional profiling approach","volume":"38","author":"L\u00fc","year":"2015","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2022.06.052_bib6","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.fsi.2016.08.013","article-title":"Transcriptome signatures in common carp spleen in response to Aeromonas hydrophila infection","volume":"57","author":"Jiang","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.06.052_bib7","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.fsi.2016.02.035","article-title":"Transcriptome profiling of grass carp (Ctenopharyngodon idellus) infected with Aeromonas hydrophila","volume":"51","author":"Yang","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.06.052_bib8","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/j.fsi.2017.06.053","article-title":"Transcriptome profiling and expression analysis of immune responsive genes in the liver of Golden mahseer (Tor putitora) challenged with Aeromonas hydrophila","volume":"67","author":"Kumar","year":"2017","journal-title":"Fish Shellfish Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2022.06.052_bib9","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.imlet.2012.08.003","article-title":"Immune system and immune responses in fish and their role in comparative immunity study: a model for higher organisms","volume":"148","author":"Rauta","year":"2012","journal-title":"Immunol. Lett."},{"issue":"5","key":"10.1016\/j.fsi.2022.06.052_bib10","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1038\/nri798","article-title":"T-cell development and the CD4\u2013CD8 lineage decision","volume":"2","author":"Germain","year":"2002","journal-title":"Nat. Rev. Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2022.06.052_bib11","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1086\/317537","article-title":"Type 1\/Type 2 immunity in infectious diseases","volume":"32","author":"Spellberg","year":"2001","journal-title":"Clin. Infect. Dis."},{"issue":"12","key":"10.1016\/j.fsi.2022.06.052_bib12","doi-asserted-by":"crossref","first-page":"4245","DOI":"10.4049\/jimmunol.140.12.4245","article-title":"Anti-proliferative effect of IFN-gamma in immune regulation. I. IFN-gamma inhibits the proliferation of Th2 but not Th1 murine helper T lymphocyte clones","volume":"140","author":"Gajewski","year":"1988","journal-title":"J. Immunol."},{"issue":"7258","key":"10.1016\/j.fsi.2022.06.052_bib13","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1136\/bmj.321.7258.424","article-title":"Th1 and Th2 responses: what are they?","volume":"321","author":"Berger","year":"2000","journal-title":"Bmj"},{"issue":"1","key":"10.1016\/j.fsi.2022.06.052_bib14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13567-018-0620-0","article-title":"CD4: a vital player in the teleost fish immune system","volume":"50","author":"Ashfaq","year":"2019","journal-title":"Vet. Res."},{"issue":"3","key":"10.1016\/j.fsi.2022.06.052_bib15","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1172\/JCI59643","article-title":"Macrophage plasticity and polarization: in vivo veritas","volume":"122","author":"Sica","year":"2012","journal-title":"J. Clin. Investig."},{"issue":"6","key":"10.1016\/j.fsi.2022.06.052_bib16","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1189\/jlb.1012512","article-title":"Macrophage phenotypes during tissue repair","volume":"93","author":"Novak","year":"2013","journal-title":"J. Leukoc. Biol."},{"key":"10.1016\/j.fsi.2022.06.052_bib17","first-page":"6","article-title":"The M1 and M2 paradigm of macrophage activation: time for reassessment","author":"Martinez","year":"2014","journal-title":"F1000prime reports"},{"issue":"6","key":"10.1016\/j.fsi.2022.06.052_bib18","doi-asserted-by":"crossref","first-page":"3733","DOI":"10.4049\/jimmunol.181.6.3733","article-title":"Macrophage polarization in bacterial infections","volume":"181","author":"Benoit","year":"2008","journal-title":"J. Immunol."},{"issue":"35","key":"10.1016\/j.fsi.2022.06.052_bib19","doi-asserted-by":"crossref","first-page":"14978","DOI":"10.1073\/pnas.0809784106","article-title":"Tolerance and M2 (alternative) macrophage polarization are related processes orchestrated by p50 nuclear factor \u03baB","volume":"106","author":"Porta","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"issue":"2","key":"10.1016\/j.fsi.2022.06.052_bib20","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1002\/path.4133","article-title":"Macrophage plasticity and polarization in tissue repair and remodelling","volume":"229","author":"Mantovani","year":"2013","journal-title":"J. Pathol."},{"issue":"2","key":"10.1016\/j.fsi.2022.06.052_bib21","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1007\/s10439-013-0941-0","article-title":"A perspective on immunomodulation and tissue repair","volume":"42","author":"Mokarram","year":"2014","journal-title":"Ann. Biomed. Eng."},{"issue":"12","key":"10.1016\/j.fsi.2022.06.052_bib22","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0145342","article-title":"Novel markers to delineate murine M1 and M2 macrophages","volume":"10","author":"Jablonski","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2022.06.052_bib23","doi-asserted-by":"crossref","first-page":"152","DOI":"10.3389\/fimmu.2020.00152","article-title":"Fish macrophages show distinct metabolic signatures upon polarization","volume":"11","author":"Wentzel","year":"2020","journal-title":"Front. Immunol."},{"issue":"5","key":"10.1016\/j.fsi.2022.06.052_bib24","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/nrg2091","article-title":"Animal models of human disease: zebrafish swim into view","volume":"8","author":"Lieschke","year":"2007","journal-title":"Nat. Rev. Genet."},{"issue":"2","key":"10.1016\/j.fsi.2022.06.052_bib25","first-page":"497","article-title":"Bacterial infections from aquatic species: potential for and prevention of contact zoonoses","volume":"32","author":"Haenen","year":"2013","journal-title":"Revue scientifique et technique (International Office of Epizootics)"},{"issue":"4","key":"10.1016\/j.fsi.2022.06.052_bib26","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.fsi.2008.05.005","article-title":"Zebrafish as a model for infectious disease and immune function","volume":"25","author":"Sullivan","year":"2008","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.06.052_bib27","series-title":"The Zebrafish Book: a Guide for the Laboratory Use of Zebrafish","author":"Westerfield","year":"2000"},{"issue":"3\u20134","key":"10.1016\/j.fsi.2022.06.052_bib28","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.dci.2012.02.005","article-title":"Aeromonas hydrophila induced head kidney macrophage apoptosis in Clarias batrachus involves the activation of calpain and is caspase-3 mediated","volume":"37","author":"Banerjee","year":"2012","journal-title":"Dev. Comp. Immunol."},{"issue":"4","key":"10.1016\/j.fsi.2022.06.052_bib29","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1004018","article-title":"Role of calmodulin-calmodulin kinase II, cAMP\/protein kinase A and ERK 1\/2 on Aeromonas hydrophila-induced apoptosis of head kidney macrophages","volume":"10","author":"Banerjee","year":"2014","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.fsi.2022.06.052_bib30","article-title":"Dissection of organs from the adult zebrafish","volume":"37","author":"Gupta","year":"2010","journal-title":"JoVE: JoVE"},{"key":"10.1016\/j.fsi.2022.06.052_bib31","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.cbpc.2017.11.003","article-title":"Comparative of fixation and embedding techniques for optimized histological preparation of zebrafish","volume":"208","author":"Copper","year":"2018","journal-title":"Comp. Biochem. Physiol. C Toxicol. Pharmacol."},{"key":"10.1016\/j.fsi.2022.06.052_bib32","doi-asserted-by":"crossref","DOI":"10.1038\/cddiscovery.2017.67","article-title":"Aeromonas hydrophila utilizes TLR4 topology for synchronous activation of MyD88 and TRIF to orchestrate anti-inflammatory responses in zebrafish","volume":"3","author":"Srivastava","year":"2017","journal-title":"Cell death Discov."},{"issue":"4","key":"10.1016\/j.fsi.2022.06.052_bib33","first-page":"254","article-title":"Pathogenic analysis of Aeromonas hydrophila septicemia","volume":"27","author":"Chang","year":"1997","journal-title":"Ann. Clin. Lab. Sci."},{"issue":"7","key":"10.1016\/j.fsi.2022.06.052_bib34","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1016\/j.dci.2007.11.011","article-title":"Immunology and zebrafish: spawning new models of human disease","volume":"32","author":"Meeker","year":"2008","journal-title":"Dev. Comp. Immunol."},{"issue":"3","key":"10.1016\/j.fsi.2022.06.052_bib35","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.fsi.2008.05.002","article-title":"Immune response of zebrafish (Danio rerio) against a newly isolated bacterial pathogen Aeromonas hydrophila","volume":"25","author":"Rodr\u00edguez","year":"2008","journal-title":"Fish Shellfish Immunol."},{"issue":"4","key":"10.1016\/j.fsi.2022.06.052_bib36","first-page":"695","article-title":"Pro-versus anti-inflammatory cytokines: myth or reality","volume":"47","author":"Cavaillon","year":"2001","journal-title":"Cell. Mol. Biol."},{"issue":"2","key":"10.1016\/j.fsi.2022.06.052_bib37","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1378\/chest.118.2.503","article-title":"Proinflammatory cytokines","volume":"118","author":"Dinarello","year":"2000","journal-title":"Chest"},{"issue":"3","key":"10.1016\/j.fsi.2022.06.052_bib38","doi-asserted-by":"crossref","first-page":"214","DOI":"10.2174\/187152309789151986","article-title":"Pathogenic bacterial proteins and their anti-inflammatory effects in the eukaryotic host","volume":"8","author":"Sun","year":"2009","journal-title":"Anti-Inflammatory Anti-Allergy Agents Med. Chem."},{"issue":"8","key":"10.1016\/j.fsi.2022.06.052_bib39","doi-asserted-by":"crossref","first-page":"2964","DOI":"10.1128\/IAI.00047-11","article-title":"Interleukin-10 and immunity against prokaryotic and eukaryotic intracellular pathogens","volume":"79","author":"Cyktor","year":"2011","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fsi.2022.06.052_bib40","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.37172","article-title":"A bacterial immunomodulatory protein with lipocalin-like domains facilitates host\u2013bacteria mutualism in larval zebrafish","volume":"7","author":"Rolig","year":"2018","journal-title":"Elife"}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464822003709?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464822003709?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T05:37:28Z","timestamp":1760938648000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464822003709"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8]]},"references-count":40,"alternative-id":["S1050464822003709"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2022.06.052","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2022,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Th1\u2013Th2 and M1-M2 interplay sculpt Aeromonas hydrophila pathogenesis in zebrafish (Danio rerio)","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2022.06.052","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}