{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T12:20:36Z","timestamp":1789906836400,"version":"4.0.1"},"reference-count":26,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31372564"],"award-info":[{"award-number":["31372564"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31672692"],"award-info":[{"award-number":["31672692"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2020MC197"],"award-info":[{"award-number":["ZR2020MC197"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014103","name":"Key Technology Research and Development Program of Shandong","doi-asserted-by":"publisher","award":["2017 NC210001"],"award-info":[{"award-number":["2017 NC210001"]}],"id":[{"id":"10.13039\/100014103","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2022,9]]},"DOI":"10.1016\/j.fsi.2022.08.033","type":"journal-article","created":{"date-parts":[[2022,8,18]],"date-time":"2022-08-18T12:19:39Z","timestamp":1660825179000},"page":"644-650","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Different antigen ratio in bivalent vaccine can affect immunological activation and protection against Aeromonas salmonicida and Vibrio anguillarum in Atlantic salmon"],"prefix":"10.1016","volume":"128","author":[{"given":"Baozhi","family":"Kuang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"You","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2616-4367","authenticated-orcid":false,"given":"Shiyong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2022.08.033_bib1","series-title":"Bacterial Fish Pathogens","isbn-type":"print","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-32674-0","author":"Austin","year":"2016","ISBN":"https:\/\/id.crossref.org\/isbn\/9783319326733"},{"key":"10.1016\/j.fsi.2022.08.033_bib2","first-page":"52","article-title":"Vibriosis and furunculosis in marine cultured salmon in Puget Sound, Washington","volume":"40","author":"Novotny","year":"1978","journal-title":"Mar. Fish. Rev."},{"key":"10.1016\/j.fsi.2022.08.033_bib3","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/BF02850735","article-title":"A Vibrio anguillarum strain associated with the ulcerative skin in cultured flounder, Paralichthy olivaceus, Chin","volume":"19","author":"Mo","year":"2001","journal-title":"J. Oceanol. Limnol."},{"key":"10.1016\/j.fsi.2022.08.033_bib4","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.fsi.2015.02.029","article-title":"The impact of Aeromonas salmonicida infection on innate immune parameters of Atlantic salmon (Salmo salar L)","volume":"44","author":"Du","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.033_bib5","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1006\/fsim.1997.0135","article-title":"Immunological cross reactions between Aeromonas salmonicida and Vibrio salmonicida in Atlantic salmon (Salmo salar L.) and rabbit","volume":"8","author":"Hoel","year":"1998","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.033_bib6","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.fsi.2019.02.014","article-title":"Synergistic effect of a combined live Vibrio anguillarum and Edwardsiella piscicida vaccine in turbot","volume":"88","author":"Bao","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.033_bib7","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1016\/j.vaccine.2017.11.074","article-title":"FlaC supplemented with VAA, OmpK or OmpR as bivalent subunit vaccine candidates induce immune responses against Vibrio anguillarum in flounder (Paralichthys olivaceus)","volume":"36","author":"Xing","year":"2018","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2022.08.033_bib8","doi-asserted-by":"crossref","first-page":"906","DOI":"10.3390\/vaccines9080906","article-title":"Effect of bivalent vaccines against Vibrio anguillarum and Aeromonas salmonicida subspecie achromogenes on health and survival of turbot","volume":"14","author":"Torres","year":"2021","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.fsi.2022.08.033_bib9","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.fsi.2006.04.010","article-title":"Multiple whole bacterial antigens in polyvalent vaccine may result in inhibition of specific responses in rainbow trout (Oncorhynchus mykiss)","volume":"22","author":"Nikoskelainen","year":"2007","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.033_bib10","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1111\/jfd.12527","article-title":"Positive correlation between Aeromonas salmonicida vaccine antigen concentration and protection in vaccinated rainbow trout Oncorhynchus mykiss evaluated by a tail fin infection model","volume":"40","author":"Marana","year":"2017","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2022.08.033_bib11","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1006\/fsim.1996.0063","article-title":"Vibrio antigens of polyvalent vaccines enhance the humoral immune response to Aeromonas salmonicida antigens in Atlantic salmon (Salmo salar L.)","volume":"7","author":"Hoel","year":"1997","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.033_bib12","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1038\/nri3151","article-title":"Accessory molecules for Toll-like receptors and their function","volume":"12","author":"Clarissa","year":"2012","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.fsi.2022.08.033_bib13","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/ni.1863","article-title":"The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors","volume":"11","author":"Kawai","year":"2010","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.fsi.2022.08.033_bib14","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.fsi.2014.09.022","article-title":"Toll-like receptor recognition of bacteria in fish: ligand specificity and signal pathways","volume":"41","author":"Zhang","year":"2014","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.033_bib15","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1016\/j.ijbiomac.2020.07.293","article-title":"Structure of fish Toll-like receptors (TLR) and NOD-like receptors (NLR)","volume":"161","author":"Sahoo","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.fsi.2022.08.033_bib16","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1007\/s00251-013-0694-9","article-title":"Comprehensive survey and genomic characterization of Toll-like receptors (TLRs) in channel catfish, Ictalurus punctatus: identification of novel fish TLRs","volume":"65","author":"Quiniou","year":"2013","journal-title":"Immunogenetics"},{"key":"10.1016\/j.fsi.2022.08.033_bib17","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.fsi.2016.06.047","article-title":"Identification and expression analysis of TLR2 in mucosal tissues of turbot (Scophthalmus maximus L.) following bacterial challenge","volume":"55","author":"Liu","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.033_bib18","doi-asserted-by":"crossref","first-page":"5292","DOI":"10.1128\/jb.179.17.5292-5299.1997","article-title":"Identification and molecular characterization of two tandemly located flagellin genes from Aeromonas salmonicida A449","volume":"179","author":"Umelo","year":"1997","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.fsi.2022.08.033_bib19","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.fsi.2012.03.031","article-title":"Immune responses of zebrafish (Danio rerio) induced by bath-vaccination with a live attenuated Vibrio anguillarum vaccine candidate","volume":"33","author":"Zhang","year":"2012","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.033_bib20","article-title":"Transcriptome profiling reveals Th17-Like immune responses induced in zebrafish bath-vaccinated with a live attenuated Vibrio anguillarum","volume":"8","author":"Zhang","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2022.08.033_bib21","doi-asserted-by":"crossref","first-page":"5937","DOI":"10.1016\/j.vaccine.2014.08.074","article-title":"A live attenuated combination vaccine evokes effective immune-mediated protection against Edwardsiella tarda and Vibrio anguillarum","volume":"32","author":"Gao","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2022.08.033_bib22","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1038\/nature06246","article-title":"Recognition of microorganisms and activation of the immune response","volume":"449","author":"Medzhitov","year":"2007","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2022.08.033_bib23","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.molimm.2018.12.018","article-title":"A time-course study of gene expression and antibody repertoire at early time post vaccination of Atlantic salmon","volume":"106","author":"Lund","year":"2019","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2022.08.033_bib24","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1006\/fsim.1998.0192","article-title":"Immunity to bacteria in fish","volume":"9","author":"Ellis","year":"1999","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.033_bib25","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1111\/j.1365-2761.2005.00617.x","article-title":"Experimental infection of turbot, Scophthalmus maximus (L.), by Aeromonas salmonicida subsp. achromogenes and evaluation of cross protection induced by a furunculosis vaccine","volume":"28","author":"Bj\u00f6rnsd\u00f3ttir","year":"2005","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2022.08.033_bib26","doi-asserted-by":"crossref","first-page":"3065","DOI":"10.1093\/bioinformatics\/btx341","article-title":"Computational modeling of immune system of the fish for a more effective vaccination in aquaculture","volume":"33","author":"Madonia","year":"2017","journal-title":"Bioinformatics"}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464822005095?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464822005095?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T05:43:33Z","timestamp":1760939013000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464822005095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9]]},"references-count":26,"alternative-id":["S1050464822005095"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2022.08.033","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2022,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Different antigen ratio in bivalent vaccine can affect immunological activation and protection against Aeromonas salmonicida and Vibrio anguillarum in Atlantic salmon","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2022.08.033","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}