{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T12:08:07Z","timestamp":1778587687826,"version":"3.51.4"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,10,1]],"date-time":"2022-10-01T00:00:00Z","timestamp":1664582400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2021A1515011222"],"award-info":[{"award-number":["2021A1515011222"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2020YFD0900201"],"award-info":[{"award-number":["2020YFD0900201"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2022,10]]},"DOI":"10.1016\/j.fsi.2022.08.044","type":"journal-article","created":{"date-parts":[[2022,8,21]],"date-time":"2022-08-21T01:22:44Z","timestamp":1661044964000},"page":"161-169","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Construction of an attenuated glutamyl endopeptidase deletion strain of Nocardia seriolae"],"prefix":"10.1016","volume":"129","author":[{"given":"Wenji","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suying","family":"Hou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianlin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6244-8060","authenticated-orcid":false,"given":"Liqun","family":"Xia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yishan","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2022.08.044_bib1","doi-asserted-by":"crossref","DOI":"10.1002\/mbo3.656","article-title":"Genomic characterization of Nocardia seriolae strains isolated from diseased fish","volume":"8","author":"Han","year":"2019","journal-title":"MicrobiologyOpen"},{"key":"10.1016\/j.fsi.2022.08.044_bib2","first-page":"75","article-title":"Nocardiosis in immune disorder disease, Malays","volume":"21","author":"Bafghi","year":"2014","journal-title":"J. Med. Sci. MJMS."},{"key":"10.1016\/j.fsi.2022.08.044_bib3","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1111\/jfd.12782","article-title":"Current knowledge of nocardiosis in teleost fish","volume":"41","author":"Maekawa","year":"2018","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2022.08.044_bib4","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.fsi.2019.10.024","article-title":"Comparison of protective efficacy between two DNA vaccines encoding DnaK and GroEL against fish nocardiosis","volume":"95","author":"Chen","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.044_bib5","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3147\/jsfp.13.25","article-title":"Nocardial infection of cultured yellowtail","volume":"13","author":"Kusuda","year":"1978","journal-title":"Fish Pathol."},{"key":"10.1016\/j.fsi.2022.08.044_bib6","first-page":"270","article-title":"Detection of antibody response against Nocardia seriolae by enzyme-linked immunosorbent assay (ELISA) and a preliminary vaccine trial in yellowtail Seriola quinqueradiata","volume":"25","author":"Shimahara","year":"2005","journal-title":"Bull. Eur. Assoc. Fish Pathol."},{"key":"10.1016\/j.fsi.2022.08.044_bib7","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1016\/j.aquaculture.2006.09.006","article-title":"Live vaccine trials against nocardiosis in yellowtail Seriola quinqueradiata","volume":"261","author":"Itano","year":"2006","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2022.08.044_bib8","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1016\/j.fsi.2020.08.013","article-title":"Immunogenicity and efficacy of two DNA vaccines encoding antigenic PspA and TerD against Nocardia seriolae in hybrid snakehead","volume":"106","author":"Chen","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.044_bib9","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.fsi.2019.09.038","article-title":"Identification of common antigens of three pathogenic Nocardia species and development of DNA vaccine against fish nocardiosis","volume":"95","author":"Chen","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.044_bib10","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.fsi.2019.12.014","article-title":"Development of DNA vaccines encoding ribosomal proteins (RplL and RpsA) against Nocardia seriolae infection in fish","volume":"96","author":"Chen","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.044_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2020.103960","article-title":"RGD-binding integrins mediated phagocytosis involved in the entry of Edwardsiella tarda into mudskipper MO\/M\u0424","volume":"116","author":"Zhu","year":"2021","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2022.08.044_bib12","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1016\/j.it.2021.06.003","article-title":"CD4+ T cells that help B cells \u2013 a proposal for uniform nomenclature","volume":"42","author":"Eisenbarth","year":"2021","journal-title":"Trends Immunol."},{"key":"10.1016\/j.fsi.2022.08.044_bib13","first-page":"563","article-title":"Glutamyl endopeptidase. Structure, function, practical use","volume":"29","author":"Mil\u2019gotina","year":"2003","journal-title":"Bioorg. Khim."},{"key":"10.1016\/j.fsi.2022.08.044_bib14","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1046\/j.1365-2958.1998.01075.x","article-title":"Staphylococcus aureus genetic loci impacting growth and survival in multiple infection environments","volume":"30","author":"Coulter","year":"1998","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.fsi.2022.08.044_bib15","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1111\/j.1574-695X.2007.00371.x","article-title":"Staphylococcus aureus infection triggers production of neutralizing, V8 protease-specific antibodies","volume":"52","author":"Calander","year":"2008","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"10.1016\/j.fsi.2022.08.044_bib16","series-title":"Handb. Proteolytic Enzym","first-page":"2534","article-title":"Glutamyl endopeptidase I","author":"Stennicke","year":"2013"},{"key":"10.1016\/j.fsi.2022.08.044_bib17","article-title":"Characterization and function study of a glutamyl endopeptidase homolog from Nocardia seriolae","author":"Wang","year":"2020","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2022.08.044_bib18","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1007\/s12274-017-1778-x","article-title":"Cell-assembled (Gd-DOTA)i-triphenylphosphonium (TPP) nanoclusters as a T2 contrast agent reveal in vivo fates of stem cell transplants","volume":"11","author":"Zhang","year":"2018","journal-title":"Nano Res."},{"key":"10.1016\/j.fsi.2022.08.044_bib19","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.fsi.2019.09.038","article-title":"Identification of common antigens of three pathogenic nocardia species and development of DNA vaccine against fish nocardiosis","volume":"95","author":"Chen","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.044_bib20","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1016\/j.fsi.2020.08.013","article-title":"Immunogenicity and efficacy of two DNA vaccines encoding antigenic PspA and TerD against Nocardia seriolae in hybrid snakehead","volume":"106","author":"Chen","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.044_bib21","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods San Diego Calif"},{"key":"10.1016\/j.fsi.2022.08.044_bib22","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/j.fsi.2018.07.039","article-title":"Transcriptome analysis of immune-related gene expression in hybrid snakehead (Channa maculata \u2640 \u00d7 Channa argus \u2642) after challenge with Nocardia seriolae","volume":"81","author":"Chen","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.044_bib23","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.fsi.2014.05.015","article-title":"Immune responses to live and inactivated Nocardia seriolae and protective effect of recombinant interferon gamma (rIFN\u03b3) against nocardiosis in ginbuna crucian carp, Carassius auratus langsdorfii","volume":"39","author":"Nayak","year":"2014","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.044_bib24","doi-asserted-by":"crossref","DOI":"10.1093\/femsle\/fnx143","article-title":"Subcellular localization and function study of a secreted phospholipase C from Nocardia seriolae","volume":"364","author":"Xia","year":"2017","journal-title":"FEMS Microbiol. Lett."},{"key":"10.1016\/j.fsi.2022.08.044_bib25","article-title":"Identification of a Nocardia seriolae secreted protein targeting host cell mitochondria and inducing apoptosis in fathead minnow (FHM) cells","author":"Chen","year":"2018","journal-title":"bioRxiv"},{"key":"10.1016\/j.fsi.2022.08.044_bib26","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1111\/jfd.13062","article-title":"Identification of a mitochondrial-targeting secretory protein from Nocardia seriolae which induces apoptosis in fathead minnow cells","volume":"42","author":"Chen","year":"2019","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2022.08.044_bib27","article-title":"Impact of individual extracellular proteases on Staphylococcus aureus biofilm formation in diverse clinical isolates and their isogenic sarA mutants","volume":"3","author":"Aj","year":"2014","journal-title":"MicrobiologyOpen"},{"key":"10.1016\/j.fsi.2022.08.044_bib28","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/s11033-006-9017-7","article-title":"The expression of Bacillus intermedius glutamyl endopeptidase gene in Bacillus subtilis recombinant strains","volume":"34","author":"Sharipova","year":"2007","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.fsi.2022.08.044_bib29","doi-asserted-by":"crossref","first-page":"176","DOI":"10.2174\/1872208310666160517115047","article-title":"A review of gene knockout strategies for microbial cells","volume":"9","author":"Tang","year":"2015","journal-title":"Recent Pat. Biotechnol."},{"key":"10.1016\/j.fsi.2022.08.044_bib30","first-page":"1105","article-title":"The effect of OmpR on the growth and replication of Brucella melitensis","volume":"40","author":"Song","year":"2018","journal-title":"Chin. J. Prev. Vet. Med."},{"key":"10.1016\/j.fsi.2022.08.044_bib31","doi-asserted-by":"crossref","first-page":"2709","DOI":"10.3390\/ijms19092709","article-title":"Discovery of lahS as a global regulator of environmental adaptation and virulence in Aeromonas hydrophila","volume":"19","author":"Dong","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fsi.2022.08.044_bib32","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1128\/IAI.66.2.771-776.1998","article-title":"Genetic inactivation of an extracellular cysteine protease (SpeB) expressed by Streptococcus pyogenes decreases resistance to phagocytosis and dissemination to organs","volume":"66","author":"Lukomski","year":"1998","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fsi.2022.08.044_bib33","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1007\/s12562-010-0239-1","article-title":"Immune response of largemouth bass, Micropterus salmoides, to whole cells of different Nocardia seriolae strains","volume":"76","author":"Shimahara","year":"2010","journal-title":"Fish. Sci."},{"key":"10.1016\/j.fsi.2022.08.044_bib34","first-page":"270","article-title":"Detection of antibody response against Nocardia seriolae by enzyme-linked immunosorbent assay (ELISA) and a preliminary vaccine trial in yellowtail Seriola quinqueradiata","volume":"25","author":"Shimahara","year":"2005","journal-title":"Bull. Eur. Assoc. Fish Pathol."},{"key":"10.1016\/j.fsi.2022.08.044_bib35","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.fsi.2012.05.002","article-title":"Mycobacterium bovis BCG vaccine induces non-specific immune responses in Japanese flounder against Nocardia seriolae","volume":"33","author":"Kato","year":"2012","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.044_bib36","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.fsi.2014.05.015","article-title":"Immune responses to live and inactivated Nocardia seriolae and protective effect of recombinant interferon gamma (rIFN\u03b3) against nocardiosis in ginbuna crucian carp, Carassius auratus langsdorfii","volume":"39","author":"Nayak","year":"2014","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.044_bib37","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.fsi.2019.08.031","article-title":"Gene expression associated with immune response in Atlantic salmon head-kidney vaccinated with inactivated whole-cell bacterin of Piscirickettsia salmonis and pathogenic isolates","volume":"93","author":"Rozas-Serri","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.08.044_bib38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13567-018-0620-0","article-title":"CD4: a vital player in the teleost fish immune system","volume":"50","author":"Ashfaq","year":"2019","journal-title":"Vet. Res."},{"key":"10.1016\/j.fsi.2022.08.044_bib39","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.dci.2019.03.017","article-title":"Functional characterization of IL-10 and its receptor subunits in a perciform fish, the Mandarin fish, Siniperca chuatsi","volume":"97","author":"Huo","year":"2019","journal-title":"Dev. Comp. Immunol."}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464822005198?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464822005198?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T11:10:20Z","timestamp":1778584220000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464822005198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10]]},"references-count":39,"alternative-id":["S1050464822005198"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2022.08.044","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2022,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Construction of an attenuated glutamyl endopeptidase deletion strain of Nocardia seriolae","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2022.08.044","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}