{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T05:08:45Z","timestamp":1776402525784,"version":"3.51.2"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T00:00:00Z","timestamp":1695772800000},"content-version":"am","delay-in-days":330,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100018067","name":"Western Regional Aquaculture Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018067","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000192","name":"National Oceanic and Atmospheric Administration","doi-asserted-by":"publisher","award":["NA14OAR4170078"],"award-info":[{"award-number":["NA14OAR4170078"]}],"id":[{"id":"10.13039\/100000192","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010784","name":"Ocean Frontier Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010784","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"USDA NIFA","doi-asserted-by":"publisher","award":["2020-38500-32561"],"award-info":[{"award-number":["2020-38500-32561"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010785","name":"Canada First Research Excellence Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010785","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007812","name":"University of Washington","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007812","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2022,11]]},"DOI":"10.1016\/j.fsi.2022.09.038","type":"journal-article","created":{"date-parts":[[2022,9,24]],"date-time":"2022-09-24T01:16:49Z","timestamp":1663982209000},"page":"479-489","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"special_numbering":"C","title":["Production of a monoclonal antibody specific to sablefish (Anoplopoma fimbria) IgM and its application in ELISA, western blotting, and immunofluorescent staining"],"prefix":"10.1016","volume":"130","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7078-8580","authenticated-orcid":false,"given":"Evan M.","family":"Jones","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luke P.","family":"Oliver","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6687-4304","authenticated-orcid":false,"given":"Robine.H.J.","family":"Leeuwis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronica","family":"Myrsell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary R.","family":"Arkoosh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph P.","family":"Dietrich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cameron M.","family":"Schuster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3072-1672","authenticated-orcid":false,"given":"Matt","family":"Hawkyard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. Kurt","family":"Gamperl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9488-0461","authenticated-orcid":false,"given":"Kenneth D.","family":"Cain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2022.09.038_bib1","author":"Hartley","year":"2020"},{"key":"10.1016\/j.fsi.2022.09.038_bib2","series-title":"Advances in US Sablefish Aquaculture","author":"Wiedenhoft","year":"2017"},{"key":"10.1016\/j.fsi.2022.09.038_bib3","series-title":"Castill0-Jordan, Status of the Sablefish Stock in U.S. Waters","author":"Haltuch","year":"2019"},{"key":"10.1016\/j.fsi.2022.09.038_bib4","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1111\/jwas.12769","article-title":"Status of sablefish, Anoplopoma fimbria, aquaculture","volume":"52","author":"Goetz","year":"2021","journal-title":"J. World Aquacult. Soc."},{"key":"10.1016\/j.fsi.2022.09.038_bib5","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.aquaculture.2017.05.037","article-title":"Establishment of monosex female production of sablefish (Anoplopoma fimbria) through direct and indirect sex control","volume":"479","author":"Luckenbach","year":"2017","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2022.09.038_bib6","doi-asserted-by":"crossref","first-page":"3032","DOI":"10.1111\/are.13135","article-title":"Optimization of fishmeal, fish oil and wheat in diets for juvenile sablefish, Anoplopoma fimbria","volume":"48","author":"Forster","year":"2017","journal-title":"Aquacult. Res."},{"key":"10.1016\/j.fsi.2022.09.038_bib7","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.aquaeng.2015.09.003","article-title":"Culture of sablefish (Anoplopoma fimbria) larvae in four experimental tank designs","volume":"69","author":"Cook","year":"2015","journal-title":"Aquacult. Eng."},{"key":"10.1016\/j.fsi.2022.09.038_bib8","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1007\/s10886-016-0713-z","article-title":"Dimethylsulfoniopropionate (DMSP) increases survival of larval sablefish, Anoplopoma fimbria","volume":"42","author":"Lee","year":"2016","journal-title":"J. Chem. Ecol."},{"key":"10.1016\/j.fsi.2022.09.038_bib9","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1006160","article-title":"New paradigms to help solve the global aquaculture disease crisis","volume":"13","author":"Stentiford","year":"2017","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.fsi.2022.09.038_bib10","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.1139\/f71-243","article-title":"An aberrant strain of the bacterial fish pathogen Aeromonas salmonicida isolated from a marine host, the sablefish (Anoplopoma fimbria), and from two species of cultured pacific salmon","volume":"28","author":"Evelyn","year":"1971","journal-title":"J. Fish. Res. Board Can."},{"key":"10.1016\/j.fsi.2022.09.038_bib11","doi-asserted-by":"crossref","first-page":"189","DOI":"10.3390\/microorganisms10010189","article-title":"Comparative genomics of typical and atypical Aeromonas salmonicida complete genomes revealed new insights into pathogenesis evolution","volume":"10","author":"Vasquez","year":"2022","journal-title":"Microorganisms"},{"key":"10.1016\/j.fsi.2022.09.038_bib12","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1111\/are.13449","article-title":"Exploring the efficacy of vaccine techniques in juvenile sablefish, Anoplopoma fimbria","volume":"49","author":"Arkoosh","year":"2018","journal-title":"Aquacult. Res."},{"key":"10.1016\/j.fsi.2022.09.038_bib13","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.fsi.2020.06.005","article-title":"Aeromonas salmonicida infection kinetics and protective immune response to vaccination in sablefish (Anoplopoma fimbria)","volume":"104","author":"Vasquez","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib14","series-title":"Climate Change May Impair a Fish's Ability to Defend against Bacterial Pathogens via Hypoxia-Mediated Effects of Adaptive Immunity","author":"Leeuwis","year":"2022"},{"key":"10.1016\/j.fsi.2022.09.038_bib15","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1016\/j.fsi.2013.10.015","article-title":"The teleost humoral immune response","volume":"35","author":"Ye","year":"2013","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2021.104079","article-title":"Mucosal immunoglobulins of teleost fish: a decade of advances","volume":"121","author":"Salinas","year":"2021","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib17","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1016\/j.dci.2011.11.009","article-title":"Mucosal immunoglobulins and B cells of teleost fish","volume":"35","author":"Salinas","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib18","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.fsi.2018.04.042","article-title":"Development of monoclonal antibodies against IgM of sea bass (Lateolabrax japonicus) and analysis of phagocytosis by mIgM+ lymphocytes","volume":"78","author":"Yang","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib19","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/j.fsi.2019.03.002","article-title":"Production of a monoclonal antibody against of muskellunge (Esox masquinongy) IgM heavy chain and its use in development of an indirect ELISA for titrating circulating antibodies against VHSV-IVB","volume":"88","author":"Faisal","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib20","doi-asserted-by":"crossref","first-page":"567","DOI":"10.3390\/biom10040567","article-title":"Development of a monoclonal antibody specific to the IgM heavy chain of bighead catfish (Clarias macrocephalus): a biomolecular tool for the detection and quantification of IgM molecules and IgM+ cells in Clarias catfish","volume":"10","author":"Bunnoy","year":"2020","journal-title":"Biomolecules"},{"key":"10.1016\/j.fsi.2022.09.038_bib21","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.fsi.2020.12.007","article-title":"Monoclonal antibody against Nile tilapia (Oreochromis niloticus) IgM heavy chain: a valuable tool for detection and quantification of IgM and IgM+ cells","volume":"110","author":"Vel\u00e1zquez","year":"2021","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib22","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.fsi.2019.05.035","article-title":"Development of monoclonal antibody against IgM of large yellow croaker (Larimichthys crocea) and characterization of IgM+ B cells","volume":"91","author":"Huang","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib23","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.fsi.2021.03.006","article-title":"Production and characterization of a mouse monoclonal antibody against smallmouth bass (Micropterus dolomieu) IgM","volume":"113","author":"Ottinger","year":"2021","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib24","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.fsi.2019.11.059","article-title":"Development of monoclonal antibodies against serum immunoglobulins from gibel carp (Carassius auratus gibelio) and their applications in serodiagnosis of inapparent infection and evaluation of vaccination strategies","volume":"96","author":"Wu","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib25","doi-asserted-by":"crossref","first-page":"4371","DOI":"10.1111\/are.15275","article-title":"Development and application of monoclonal antibody against IgM of hybrid snakehead (Channa maculata \u2640 \u00d7 Channa argus \u2642)","volume":"52","author":"Gong","year":"2021","journal-title":"Aquacult. Res."},{"key":"10.1016\/j.fsi.2022.09.038_bib26","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1128\/CDLI.10.5.876-881.2003","article-title":"Systemic and cutaneous mucus antibody responses of channel catfish immunized against the Protozoan parasite ichthyophthirius multifiliis","volume":"10","author":"Maki","year":"2003","journal-title":"Clin. Vaccine Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib27","article-title":"Production of monoclonal antibodies","volume":"102","author":"Yokoyama","year":"2013","journal-title":"Curr. Protoc. Im."},{"key":"10.1016\/j.fsi.2022.09.038_bib28","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1016\/j.fsi.2013.08.009","article-title":"Enhanced efficacy of an attenuated Flavobacterium psychrophilum strain cultured under iron-limited conditions","volume":"35","author":"Long","year":"2013","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib29","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1046\/j.1365-2761.2002.00424.x","article-title":"Characterization of serum and mucosal antibody responses and relative per cent survival in rainbow trout, Oncorhynchus mykiss (Walbaum), following immunization and challenge with Flavobacterium psychrophilum","volume":"25","author":"LaFrentz","year":"2002","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2022.09.038_bib30","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1002\/eji.1830130706","article-title":"Lymphocyte heterogeneity in the trout,Salmo gairdneri, defined with monoclonal antibodies to IgM","volume":"13","author":"Deluca","year":"1983","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib31","series-title":"Monoclonal Antibody against Burbot (Lota Lota Maculosa) IgM, Manuscript in Preparation","author":"Oliver","year":"2022"},{"key":"10.1016\/j.fsi.2022.09.038_bib32","series-title":"The ELISA Guidebook","author":"Crowther","year":"2000"},{"key":"10.1016\/j.fsi.2022.09.038_bib33","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0003-2697(82)90673-X","article-title":"A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels","volume":"119","author":"Tsai","year":"1982","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.fsi.2022.09.038_bib34","series-title":"R: A Language and Environment for Statistical Computing","year":"2020"},{"key":"10.1016\/j.fsi.2022.09.038_bib35","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.fsi.2018.04.016","article-title":"Under control: the innate immunity of fish from the inhibitors' perspective","volume":"77","author":"Rebl","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib36","author":"Nakanishi"},{"key":"10.1016\/j.fsi.2022.09.038_bib37","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/0145-305X(88)90052-3","article-title":"Purification and partial characterization of an IgM-like serum immunoglobulin from Atlantic salmon (Salmo salar)","volume":"12","author":"H\u00e5varstein","year":"1988","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib38","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/0165-2427(90)90134-E","article-title":"Characterization of monoclonal antibodies to channel catfish, Ictalurus punctatus, leucocytes","volume":"26","author":"Ainsworth","year":"1990","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.fsi.2022.09.038_bib39","author":"Mashoof"},{"key":"10.1016\/j.fsi.2022.09.038_bib40","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0246982","article-title":"Sablefish (Anoplopoma fimbra Pallas, 1814) plasma biochemistry and hematology reference intervals including blood cell morphology","volume":"16","author":"Schubiger","year":"2021","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2022.09.038_bib41","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1080\/08997659.2015.1019159","article-title":"Pathogenicity of members of the vibrionaceae family to cultured juvenile sablefish","volume":"27","author":"Arkoosh","year":"2015","journal-title":"J. Aquat. Anim. Health"},{"key":"10.1016\/j.fsi.2022.09.038_bib42","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.fsi.2008.11.009","article-title":"Characterization of IgM of Indian major carps and their cross-reactivity with anti-fish IgM antibodies","volume":"26","author":"Bag","year":"2009","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib43","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1134\/S0032945214020131","article-title":"New data on the range, biology, and abundance of skilfish Erilepis zonifer (Anoplopomatidae)","volume":"54","author":"Zolotov","year":"2014","journal-title":"J. Ichthyol."},{"key":"10.1016\/j.fsi.2022.09.038_bib44","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1016\/j.dci.2011.04.011","article-title":"A holistic view of the dynamisms of teleost IgM: a case study of Streptococcus iniae vaccinated rainbow trout (Oncorhynchus mykiss)","volume":"36","author":"Costa","year":"2012","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2022.09.038_bib45","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1577\/1548-8667(1990)002<0135:DOAIIR>2.3.CO;2","article-title":"Detection of antibodies induced in rainbow trout by different Aeromonas salmonicida vaccine preparations","volume":"2","author":"Hastings","year":"1990","journal-title":"J. Aquat. Anim. Health"},{"key":"10.1016\/j.fsi.2022.09.038_bib46","doi-asserted-by":"crossref","first-page":"3203","DOI":"10.1002\/pmic.200300844","article-title":"Rapid screening of highly efficient vaccine candidates by immunoproteomics","volume":"4","author":"Chen","year":"2004","journal-title":"Proteomics"},{"key":"10.1016\/j.fsi.2022.09.038_bib47","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1111\/jfd.12902","article-title":"Assessment of cross\u2010protection to heterologous strains of Flavobacterium psychrophilum following vaccination with a live\u2010attenuated coldwater disease immersion vaccine","volume":"42","author":"Ma","year":"2019","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2022.09.038_bib48","article-title":"Recent advances on phagocytic B cells in teleost fish","volume":"11","author":"Wu","year":"2020","journal-title":"Front. Immunol."}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464822006040?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464822006040?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T05:41:02Z","timestamp":1760938862000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464822006040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11]]},"references-count":48,"alternative-id":["S1050464822006040"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2022.09.038","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2022,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Production of a monoclonal antibody specific to sablefish (Anoplopoma fimbria) IgM and its application in ELISA, western blotting, and immunofluorescent staining","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2022.09.038","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2022 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}