{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T11:05:08Z","timestamp":1772881508583,"version":"3.50.1"},"reference-count":48,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T00:00:00Z","timestamp":1711929600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2023M733697"],"award-info":[{"award-number":["2023M733697"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010877","name":"Science, Technology and Innovation Commission of Shenzhen Municipality","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010877","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017610","name":"Shenzhen Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["JCYJ20220530162006015"],"award-info":[{"award-number":["JCYJ20220530162006015"]}],"id":[{"id":"10.13039\/501100017610","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017610","name":"Shenzhen Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["KCXFZ20211020165547010"],"award-info":[{"award-number":["KCXFZ20211020165547010"]}],"id":[{"id":"10.13039\/501100017610","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2024,4]]},"DOI":"10.1016\/j.fsi.2024.109410","type":"journal-article","created":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T01:10:10Z","timestamp":1706836210000},"page":"109410","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"special_numbering":"C","title":["Identifying the in vivo-induced antigenic genes is a strategy to develop DNA vaccine against Nocardia seriolae in hybrid snakehead (Channa maculata \u2640 \u00d7 Channa argus \u2642)"],"prefix":"10.1016","volume":"147","author":[{"given":"Tingting","family":"Weng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoquan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanna","family":"Sirimanapong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianlin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liqun","family":"Xia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2024.109410_bib1","article-title":"A review on the pathogenic bacterium Nocardia seriolae: aetiology, pathogenesis, diagnosis and vaccine development","author":"Liu","year":"2022","journal-title":"Rev. Aquacult."},{"key":"10.1016\/j.fsi.2024.109410_bib2","doi-asserted-by":"crossref","first-page":"e01606","DOI":"10.1128\/genomeA.01606-15","article-title":"Draft genome sequence of erythromycin and oxytetracycline-sensitive Nocardia seriolae strain U-1 (NBRC 110359)","volume":"4","author":"Imajoh","year":"2016","journal-title":"Genome Announc."},{"key":"10.1016\/j.fsi.2024.109410_bib3","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1046\/j.1365-2761.2000.00217.x","article-title":"Nocardiosis in sea bass, Lateolabrax japonicus, in Taiwan","volume":"23","author":"Chen","year":"2000","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2024.109410_bib4","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.aquaculture.2007.06.019","article-title":"Nocardiosis in snakehead, Ophiocephalus argus cantor","volume":"271","author":"Wang","year":"2007","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2024.109410_bib5","doi-asserted-by":"crossref","first-page":"13","DOI":"10.3354\/dao03517","article-title":"Nocardia seriolae: a serious threat to the largemouth bass Micropterus salmoides industry in Southwest China","volume":"142","author":"Lei","year":"2020","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.fsi.2024.109410_bib6","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1007\/s10499-017-0184-4","article-title":"Nocardia seriolae infection in cultured jade perch","volume":"25","author":"Wang","year":"2017","journal-title":"Scortum barcoo. Aquac. Int."},{"issue":"3","key":"10.1016\/j.fsi.2024.109410_bib7","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1111\/jfd.12782","article-title":"Current knowledge of nocardiosis in teleost fish","volume":"41","author":"Maekawa","year":"2018","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2024.109410_bib8","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1016\/j.fsi.2020.08.013","article-title":"Immunogenicity and efficacy of two DNA vaccines encoding antigenic PspA and TerD against Nocardia seriolae in hybrid snakehead","volume":"106","author":"Chen","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib9","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.fsi.2021.01.015","article-title":"Recombinant resuscitation-promoting factor protein of Nocardia seriolae, a promissing vaccine candidate for largemouth bass (Micropterus salmoides)","volume":"111","author":"Hoang","year":"2021","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib10","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.fsi.2016.04.089","article-title":"Induction of cell-mediated immune responses after vaccination with inactivated oil-emulsion Nocardia seriolae in amberjack Seriola dumerili","volume":"53","author":"Araki","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib11","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.fsi.2017.06.025","article-title":"Adjuvant effect of recombinant interleukin-12 in the Nocardiosis formalin-killed vaccine of the amberjack Seriola dumerili","volume":"67","author":"Matsumoto","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib12","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1016\/j.aquaculture.2006.09.006","article-title":"Live vaccine trials against nocardiosis in yellowtail Seriola quinqueradiata","volume":"261","author":"Itano","year":"2006","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2024.109410_bib13","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.1040336","article-title":"Current status and development prospects of aquatic vaccines","volume":"13","author":"Du","year":"2022","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib14","doi-asserted-by":"crossref","DOI":"10.1155\/2018\/5432497","article-title":"Maintenance of fish health in aquaculture: review of epidemiological approaches for prevention and control of infectious disease of fish","volume":"2018","author":"Assefa","year":"2018","journal-title":"Vet. Med. Int."},{"key":"10.1016\/j.fsi.2024.109410_bib15","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.fsi.2019.04.066","article-title":"Progress, challenges and opportunities in fish vaccine development","volume":"90","author":"Adams","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib16","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/j.fsi.2015.05.045","article-title":"An oral DNA vaccine against infectious haematopoietic necrosis virus (IHNV) encapsulated in alginate microspheres induces dose-dependent immune responses and significant protection in rainbow trout (Oncorrhynchus mykiss)","volume":"45","author":"Ballesteros","year":"2015","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib17","doi-asserted-by":"crossref","first-page":"165","DOI":"10.3147\/jsfp.49.165","article-title":"Development of DNA vaccines against Nocardia seriolae infection in Fish","volume":"49","author":"Kato","year":"2014","journal-title":"Fish Pathol."},{"key":"10.1016\/j.fsi.2024.109410_bib18","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.fsi.2019.12.014","article-title":"Development of DNA vaccines encoding ribosomal proteins (RplL and RpsA) against Nocardia seriolae infection in fish","volume":"96","author":"Chen","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib19","unstructured":"In vivo induced antigen technology (IVIAT) - Rollins - 2005 - Cell. Microbiol. - Wiley Online Library n.d. https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1462-5822.2004.00477.x (accessed August 9, 2023)."},{"key":"10.1016\/j.fsi.2024.109410_bib20","article-title":"Identification of group A Streptococcus antigenic determinants upregulated in vivo","volume":"73","author":"Kowthar","year":"2005","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fsi.2024.109410_bib21","unstructured":"Use of in vivo-induced antigen technology for identification of Escherichia coli O157:H7 proteins expressed during human infection | Infect. Immun.. n.d. https:\/\/journals.asm.org\/doi\/10.1128\/IAI.73.5.2665-2679.2005 (accessed August 9, 2023)."},{"key":"10.1016\/j.fsi.2024.109410_bib22","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1054\/tube.2002.0337","article-title":"Selective identification of new therapeutic targets of Mycobacterium tuberculosis by IVIAT approach","volume":"82","author":"Deb","year":"2002","journal-title":"Tuberculosis"},{"key":"10.1016\/j.fsi.2024.109410_bib23","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1046\/j.1365-2958.2003.03521.x","article-title":"Identification of Candida albicans genes induced during thrush offers insight into pathogenesis: gene expression by Candida albicans during thrush","volume":"48","author":"Cheng","year":"2003","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.fsi.2024.109410_bib24","doi-asserted-by":"crossref","first-page":"8508","DOI":"10.1073\/pnas.1431769100","article-title":"Use of in vivo -induced antigen technology (IVIAT) to identify genes uniquely expressed during human infection with Vibrio cholerae","volume":"100","author":"Hang","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.fsi.2024.109410_bib25","unstructured":"Characterization and pathogenic significance of Vibrio vulnificus antigens preferentially expressed in septicemic patients | Infect. Immun.. https:\/\/journals.asm.org\/doi\/10.1128\/IAI.71.10.5461-5471.2003 (accessed August 9, 2023)."},{"key":"10.1016\/j.fsi.2024.109410_bib33","first-page":"90","article-title":"Advance in screening technologies of in vivo-induced antigen of bacteria","volume":"32","author":"Wu","year":"2011","journal-title":"Prev. Vet. Med."},{"key":"10.1016\/j.fsi.2024.109410_bib26","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/978-1-59259-948-6_7","article-title":"High-throughput cloning for proteomics research","volume":"310","author":"Doyle","year":"2005","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.fsi.2024.109410_bib31","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.fsi.2019.10.024","article-title":"Comparison of protective efficacy between two DNA vaccines encoding DnaK and GroEL against fish nocardiosis","volume":"95","author":"Chen","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib27","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-35475-4","article-title":"Regulation and overexpression studies of yidC in Mycobacterium tuberculosis","volume":"8","author":"Thakur","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fsi.2024.109410_bib28","unstructured":"The preprotein translocase YidC controls respiratory metabolism in Mycobacterium tuberculosis | Sci. Rep. https:\/\/www.nature.com\/articles\/srep24998 (accessed August 18, 2023)."},{"key":"10.1016\/j.fsi.2024.109410_bib29","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.fsi.2017.05.021","article-title":"Immunogenicity and efficacy of DNA vaccine encoding antigenic AcfA via addition of the molecular adjuvant Myd88 against Vibrio alginolyticus in Epinephelus coioides","volume":"66","author":"Huang","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib30","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.fsi.2019.09.038","article-title":"Identification of common antigens of three pathogenic Nocardia species and development of DNA vaccine against fish nocardiosis","volume":"95","author":"Chen","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib32","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-Time quantitative PCR and the 2\u2212\u0394\u0394CT Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2024.109410_bib34","first-page":"327","article-title":"In vivo induced antigen technology (IVIAT) and change mediated antigen technology (CMAT)","volume":"6","author":"Handfield","year":"2006","journal-title":"Infect. Disord.: Drug Targets"},{"key":"10.1016\/j.fsi.2024.109410_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2020.108650","article-title":"Identification of Haemophilus parasuis genes uniquely expressed during infection using in vivo-induced antigen technology","volume":"243","author":"Mao","year":"2020","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.fsi.2024.109410_bib36","unstructured":"Use of in vivo-induced antigen technology to identify genes from Aeromonas salmonicida subsp. salmonicida that are specifically expressed during infection of the rainbow trout Oncorhynchus mykiss | BMC Vet. Res. | Full Text n.d. https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-014-0298-0 (accessed October 2, 2023)."},{"key":"10.1016\/j.fsi.2024.109410_bib37","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.fsi.2009.08.006","article-title":"Identification and immunoprotective analysis of an in vivo-induced Edwardsiella tarda antigen","volume":"27","author":"Jiao","year":"2009","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib38","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1016\/j.str.2015.08.002","article-title":"Membrane insertases are present in all three domains of life","volume":"23","author":"Dalbey","year":"2015","journal-title":"Structure"},{"key":"10.1016\/j.fsi.2024.109410_bib39","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1007\/s10930-019-09847-2","article-title":"The principles of protein targeting and transport across cell membranes","volume":"38","author":"Chen","year":"2019","journal-title":"Protein J."},{"key":"10.1016\/j.fsi.2024.109410_bib40","doi-asserted-by":"crossref","first-page":"5801","DOI":"10.1073\/pnas.0636761100","article-title":"A conserved function of YidC in the biogenesis of respiratory chain complexes","volume":"100","author":"van der Laan","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.fsi.2024.109410_bib41","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1111\/mmi.13586","article-title":"Membrane protein insertase YidC in bacteria and archaea","volume":"103","author":"Kuhn","year":"2017","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.fsi.2024.109410_bib42","first-page":"13","article-title":"Intracellular behavior of Nocardia seriolae and its apoptotic effect on RAW264.7 macrophages","author":"Liu","year":"2023","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.fsi.2024.109410_bib43","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/j.dci.2011.03.007","article-title":"Advances in fish vaccine delivery","volume":"35","author":"Plant","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib44","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.fsi.2008.03.007","article-title":"What happens to the DNA vaccine in fish? A review of current knowledge","volume":"25","author":"Tonheim","year":"2008","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib45","first-page":"175","article-title":"Biotechnology and DNA vaccines for aquatic animals","volume":"27","author":"Kurath","year":"2008","journal-title":"Natl. Sci. Rev."},{"key":"10.1016\/j.fsi.2024.109410_bib46","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.fsi.2014.07.021","article-title":"Optimisation and standardisation of functional immune assays for striped catfish (Pangasianodon hypophthalmus) to compare their immune response to live and heat killed Aeromonas hydrophila as models of infection and vaccination","volume":"40","author":"Sirimanapong","year":"2014","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib47","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.fsi.2018.05.029","article-title":"Immune response and protective effect against spring viremia of carp virus induced by intramuscular vaccination with a SWCNTs-DNA vaccine encoding matrix protein","volume":"79","author":"Zhang","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109410_bib48","doi-asserted-by":"crossref","first-page":"7328","DOI":"10.1016\/j.vaccine.2015.10.078","article-title":"A cytomegalovirus DNA vaccine induces antibodies that block viral entry into fibroblasts and epithelial cells","volume":"33","author":"McVoy","year":"2015","journal-title":"Vaccine"}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464824000548?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464824000548?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T19:53:51Z","timestamp":1766778831000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464824000548"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":48,"alternative-id":["S1050464824000548"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2024.109410","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identifying the in vivo-induced antigenic genes is a strategy to develop DNA vaccine against Nocardia seriolae in hybrid snakehead (Channa maculata \u2640 \u00d7 Channa argus \u2642)","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2024.109410","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"109410"}}