{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:28:11Z","timestamp":1789586891861,"version":"build-2803163510"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T00:00:00Z","timestamp":1728000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008425","name":"Government of Galicia Department of Education Science Universities and Professional Training","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008425","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"State Agency of Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2024,11]]},"DOI":"10.1016\/j.fsi.2024.109943","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T21:50:24Z","timestamp":1728078624000},"page":"109943","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"special_numbering":"C","title":["Outer membrane vesicles (OMVs) from Tenacibaculum maritimum as a potential vaccine against fish tenacibaculosis"],"prefix":"10.1016","volume":"154","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2363-8694","authenticated-orcid":false,"given":"M. Pilar","family":"Escribano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5702-4631","authenticated-orcid":false,"given":"Miguel","family":"Balado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia E.","family":"Toranzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel L.","family":"Lemos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Magari\u00f1os","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2024.109943_bib1","doi-asserted-by":"crossref","DOI":"10.1155\/2021\/1490732","article-title":"Outer membrane vesicles (OMVs) produced by gram-negative bacteria: structure, functions, biogenesis, and vaccine application","volume":"2021","author":"Furuyama","year":"2021","journal-title":"BioMed Res. Int."},{"key":"10.1016\/j.fsi.2024.109943_bib2","doi-asserted-by":"crossref","first-page":"3424","DOI":"10.1002\/pmic.201000212","article-title":"Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa","volume":"11","author":"Choi","year":"2011","journal-title":"Proteomics"},{"key":"10.1016\/j.fsi.2024.109943_bib3","series-title":"New Frontiers : Extracellular Vesicles","author":"Mathivanan","year":"2021"},{"issue":"10","key":"10.1016\/j.fsi.2024.109943_bib4","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1038\/nrmicro3525","article-title":"Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions","volume":"13","author":"Schwechheimer","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"issue":"9","key":"10.1016\/j.fsi.2024.109943_bib5","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1080\/09168451.2019.1608809","article-title":"Bacterial communication through membrane vesicles","volume":"83","author":"Toyofuku","year":"2019","journal-title":"Biosci. Biotechnol. Biochem."},{"issue":"1","key":"10.1016\/j.fsi.2024.109943_bib6","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1128\/MMBR.00031-09","article-title":"Virulence and immunomodulatory roles of bacterial outer membrane vesicles","volume":"74","author":"Ellis","year":"2010","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"10.1016\/j.fsi.2024.109943_bib7","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1146\/annurev-micro-052821-031444","article-title":"Bacterial outer membrane vesicles: from discovery to applications","volume":"75","author":"Sartorio","year":"2021","journal-title":"Annu. Rev. Microbiol."},{"issue":"8","key":"10.1016\/j.fsi.2024.109943_bib8","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0134478","article-title":"Production of a Shigella sonnei vaccine based on generalized modules for membrane antigens (GMMA), 1790GAHB","volume":"10","author":"Gerke","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2024.109943_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.smim.2020.101433","article-title":"Outer membrane vesicle vaccines","volume":"50","author":"Micoli","year":"2020","journal-title":"Semin. Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2024.109943_bib10","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1007\/s40265-013-0155-7","article-title":"A multi-component meningococcal serogroup B vaccine (4CMenB): the clinical development program","volume":"74","author":"O'Ryan","year":"2014","journal-title":"Drugs"},{"issue":"10","key":"10.1016\/j.fsi.2024.109943_bib11","doi-asserted-by":"crossref","first-page":"4554","DOI":"10.1128\/IAI.00532-08","article-title":"Immunization with Vibrio cholerae outer membrane vesicles induces protective immunity in mice","volume":"76","author":"Schild","year":"2008","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fsi.2024.109943_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.nano.2019.102148","article-title":"Nanoparticle reinforced bacterial outer-membrane vesicles effectively prevent fatal infection of carbapenem-resistant Klebsiella pneumoniae","volume":"24","author":"Wu","year":"2020","journal-title":"Nanomed. Nanotechnol. Biol. Med."},{"issue":"1","key":"10.1016\/j.fsi.2024.109943_bib13","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.fsi.2014.10.025","article-title":"Vaccination with outer membrane vesicles from Francisella noatunensis reduces development of francisellosis in a zebrafish model","volume":"42","author":"Brudal","year":"2015","journal-title":"Fish Shellfish Immunol."},{"issue":"3","key":"10.1016\/j.fsi.2024.109943_bib14","article-title":"Outer membrane vesicles as a candidate vaccine against edwardsiellosis","volume":"8","author":"Park","year":"2011","journal-title":"PLoS One"},{"issue":"5138","key":"10.1016\/j.fsi.2024.109943_bib15","article-title":"Characterization and vaccine potential of outer membrane vesicles from Photobacterium damselae subsp","volume":"24","author":"Teixeira","year":"2023","journal-title":"piscicida. International Journal of Molecular Sciences"},{"issue":"3","key":"10.1016\/j.fsi.2024.109943_bib16","doi-asserted-by":"crossref","first-page":"255","DOI":"10.3354\/dao071255","article-title":"Tenacibaculosis infection in marine fish caused by Tenacibaculum maritimum: a review","volume":"71","author":"Avenda\u00f1o-Herrera","year":"2006","journal-title":"Dis. Aquat. Org."},{"issue":"11","key":"10.1016\/j.fsi.2024.109943_bib17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0206951","article-title":"Pathology of experimentally induced mouthrot caused by Tenacibaculum maritimum in Atlantic salmon smolts","volume":"13","author":"Frisch","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2024.109943_bib18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fcimb.2022.1068000","article-title":"Tenacibaculosis caused by Tenacibaculum maritimum: updated knowledge of this marine bacterial fish pathogen","volume":"12","author":"Mabrok","year":"2023","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.fsi.2024.109943_bib19","series-title":"Tenacibaculum maritimum: estudio epidemiol\u00f3gico y de la base gen\u00e9tica del proceso infeccioso en peces marinos","author":"Santos","year":"2022"},{"issue":"10","key":"10.1016\/j.fsi.2024.109943_bib20","doi-asserted-by":"crossref","first-page":"605","DOI":"10.47853\/FAS.2023.e52","article-title":"Development of a trivalent vaccine for prevention of co-infection by Miamiensis avidus and Tenacibaculum maritimum in farmed olive flounder","volume":"26","author":"Sohn","year":"2023","journal-title":"Fisheries and Aquatic Sciences"},{"issue":"8","key":"10.1016\/j.fsi.2024.109943_bib21","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1111\/j.1365-2761.2009.01032.x","article-title":"Effect of extracellular products of Tenacibaculum maritimum in atlantic salmon, Salmo salar L","volume":"32","author":"Van Gelderen","year":"2009","journal-title":"J. Fish. Dis."},{"issue":"1\u20132","key":"10.1016\/j.fsi.2024.109943_bib22","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.aquaculture.2008.11.012","article-title":"Experimental vaccination of Atlantic salmon (Salmo salar L.) against marine flexibacteriosis","volume":"288","author":"Van Gelderen","year":"2009","journal-title":"Aquaculture"},{"issue":"2","key":"10.1016\/j.fsi.2024.109943_bib23","doi-asserted-by":"crossref","first-page":"105","DOI":"10.3147\/jsfp.29.105","article-title":"Comparative study on Flexibacter maritimus strains isolated from farmed sea bass (Dicentrarchus labrax) in France","volume":"29","author":"Bernardet","year":"1994","journal-title":"Fish Pathol."},{"key":"10.1016\/j.fsi.2024.109943_bib24","series-title":"Flexibacter Maritimus: Estudio Fenot\u00edpico, Inmunol\u00f3gico Y Molecular","author":"Pazos","year":"1997"},{"issue":"2","key":"10.1016\/j.fsi.2024.109943_bib25","first-page":"70","article-title":"A novel O-serotype in Tenacibaculum maritimum strains isolated from cultured sole (Solea senegalensis)","volume":"25","author":"Avenda\u00f1o-Herrera","year":"2005","journal-title":"Bull. Eur. Assoc. Fish Pathol."},{"issue":"6","key":"10.1016\/j.fsi.2024.109943_bib26","first-page":"229","article-title":"Reassessment of the Tenacibaculum maritimum serotypes causing mortalities in cultured marine fish","volume":"27","author":"Castro","year":"2007","journal-title":"Bull. Eur. Assoc. Fish Pathol."},{"key":"10.1016\/j.fsi.2024.109943_bib27","series-title":"Genomic Characterization of Tenacibaculum Maritimum O\u2010antigen Gene Cluster and Development of a Multiplex PCR\u2010based Serotyping Scheme","author":"Lopez","year":"2022"},{"issue":"June","key":"10.1016\/j.fsi.2024.109943_bib28","article-title":"The secretome of the fish pathogen Tenacibaculum maritimum includes soluble virulence-related proteins and outer membrane vesicles","volume":"13","author":"Escribano","year":"2023","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.fsi.2024.109943_bib29","first-page":"1","article-title":"In vitro and in vivo activities of Flexibacter maritimus toxins","volume":"10","author":"Baxa","year":"1988","journal-title":"Reports of the USA Marine Biological"},{"key":"10.1016\/j.fsi.2024.109943_bib30","doi-asserted-by":"crossref","first-page":"103","DOI":"10.3354\/dao021103","article-title":"Response of Pasteurella piscicida and Flexibacter maritimus to skin mucus of marine fish","volume":"21","author":"Magari\u00f1os","year":"1995","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.fsi.2024.109943_bib31","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2017.01542","article-title":"The complete genome sequence of the fish pathogen Tenacibaculum maritimum provides insights into virulence mechanisms","volume":"8","author":"P\u00e9rez-Pascual","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.fsi.2024.109943_bib32","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2020.589115","article-title":"Application of TonB-dependent transporters in vaccine development of gram-negative bacteria","volume":"10","author":"Wang","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"3","key":"10.1016\/j.fsi.2024.109943_bib33","doi-asserted-by":"crossref","first-page":"211","DOI":"10.7589\/0090-3558-21.3.211","article-title":"Detection of Vibrio anguillarum antigen by the dot blot assay","volume":"21","author":"Cipriano","year":"1985","journal-title":"J. Wildl. Dis."},{"issue":"1","key":"10.1016\/j.fsi.2024.109943_bib34","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1577\/1548-8667(1990)002<0021:SRAVAS>2.3.CO;2","article-title":"Serological relationships among Vibrio anguillarum strains","volume":"2","author":"Bolinches","year":"1990","journal-title":"J. Aquat. Anim. Health"},{"issue":"3","key":"10.1016\/j.fsi.2024.109943_bib35","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1128\/aem.51.3.593-597.1986","article-title":"Serotyping of Vibrio anguillarum","volume":"51","author":"S\u00f8rensen","year":"1986","journal-title":"Appl. Environ. Microbiol."},{"issue":"3","key":"10.1016\/j.fsi.2024.109943_bib36","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty percent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Epidemiol."},{"issue":"16","key":"10.1016\/j.fsi.2024.109943_bib37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/ijms241612944","article-title":"Early molecular immune responses of turbot (Scophthalmus maximus L.) following infection with Aeromonas salmonicida subsp. salmonicida","volume":"24","author":"Fajardo","year":"2023","journal-title":"Int. J. Mol. Sci."},{"issue":"1","key":"10.1016\/j.fsi.2024.109943_bib38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2164-15-648","article-title":"Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset","volume":"15","author":"Robledo","year":"2014","journal-title":"BMC Genom."},{"key":"10.1016\/j.fsi.2024.109943_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2022.108461","article-title":"Comparative transcriptome analysis reveals the immune response of turbot (Scophthalmus maximus) induced by inactivated bivalent vaccine","volume":"132","author":"Zhang","year":"2023","journal-title":"Fish Shellfish Immunol."},{"issue":"4","key":"10.1016\/j.fsi.2024.109943_bib40","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2024.109943_bib41","doi-asserted-by":"crossref","first-page":"4591","DOI":"10.1128\/AEM.00428-15","article-title":"Extracellular vesicles of the hyperthermophilic archaeon \u201cThermococcus onnurineus\u201d NA1 T","volume":"81","author":"Choi","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"issue":"11","key":"10.1016\/j.fsi.2024.109943_bib42","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens10111439","article-title":"Experimental induction of tenacibaculosis in atlantic salmon (Salmo salar l.) using Tenacibaculum maritimum, T. dicentrarchi, and T. finnmarkense","volume":"10","author":"Nowlan","year":"2021","journal-title":"Pathogens"},{"key":"10.1016\/j.fsi.2024.109943_bib43","first-page":"1","article-title":"Experimental induction of mouthrot in Atlantic salmon smolts using Tenacibaculum maritimum from Western Canada","author":"Frisch","year":"2018","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2024.109943_bib44","series-title":"Vaccination of Atlantic salmon (Salmo salar) Against Marine FlexibacteriosisPhD Thesis","author":"Gelderen","year":"2007"},{"key":"10.1016\/j.fsi.2024.109943_bib45","first-page":"1","article-title":"Flexibacter maritimus, causal agent of flexibacteriosis in marine fish","volume":"vol. 55","author":"Santos","year":"1999"},{"key":"10.1016\/j.fsi.2024.109943_bib46","doi-asserted-by":"crossref","DOI":"10.1146\/annurev-micro-032421-111116","article-title":"TonB-dependent transport across the bacterial outer membrane","author":"Silale","year":"2023","journal-title":"Annu. Rev. Microbiol."},{"issue":"5","key":"10.1016\/j.fsi.2024.109943_bib47","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.biotechadv.2017.05.003","article-title":"Bioengineering bacterial outer membrane vesicles as vaccine platform","volume":"35","author":"Gerritzen","year":"2017","journal-title":"Biotechnol. Adv."},{"issue":"22","key":"10.1016\/j.fsi.2024.109943_bib48","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1172\/jci.insight.163347","article-title":"Consecutive BNT162b2 mRNA vaccination induces short-term epigenetic memory in innate immune cells","volume":"7","author":"Yamaguchi","year":"2022","journal-title":"JCI Insight"},{"issue":"11","key":"10.1016\/j.fsi.2024.109943_bib49","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1111\/jfd.12199","article-title":"Immunohistochemical diagnosis of tenacibaculosis in paraffin-embedded tissues of Senegalese sole Solea senegalensis Kaup, 1858","volume":"37","author":"Fa\u00edlde","year":"2014","journal-title":"J. Fish. Dis."},{"issue":"February","key":"10.1016\/j.fsi.2024.109943_bib50","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1016\/j.fsi.2019.02.015","article-title":"Mucosal and systemic immune responses in Senegalese sole (Solea senegalensis Kaup) bath challenged with Tenacibaculum maritimum: a time-course study","volume":"87","author":"Guardiola","year":"2019","journal-title":"Fish Shellfish Immunol."},{"issue":"May","key":"10.1016\/j.fsi.2024.109943_bib51","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.fsi.2020.05.080","article-title":"Mucosal immune responses in Senegalese sole (Solea senegalensis) juveniles after Tenacibaculum maritimum challenge: a comparative study between ocular and blind sides","volume":"104","author":"Escribano","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2024.109943_bib52","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1254677","article-title":"Early innate immune responses in European sea bass (Dicentrarchus labrax L.) following Tenacibaculum maritimum infection","volume":"14","author":"Ferreira","year":"2023","journal-title":"Front. Immunol."}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464824005886?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464824005886?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T23:51:17Z","timestamp":1752623477000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464824005886"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11]]},"references-count":52,"alternative-id":["S1050464824005886"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2024.109943","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2024,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Outer membrane vesicles (OMVs) from Tenacibaculum maritimum as a potential vaccine against fish tenacibaculosis","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2024.109943","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"109943"}}