{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:01:47Z","timestamp":1790348507443,"version":"4.1.0"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T00:00:00Z","timestamp":1745798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002924","name":"Federaci\u00f3n Espa\u00f1ola de Enfermedades Raras","doi-asserted-by":"publisher","award":["2014SGR-345 AGAUR"],"award-info":[{"award-number":["2014SGR-345 AGAUR"]}],"id":[{"id":"10.13039\/501100002924","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003030","name":"AGAUR","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003030","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003339","name":"Spanish Scientific Research Council","doi-asserted-by":"publisher","award":["AGL2015-65129-R"],"award-info":[{"award-number":["AGL2015-65129-R"]}],"id":[{"id":"10.13039\/501100003339","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"European Research Council","doi-asserted-by":"publisher","award":["GA639249"],"award-info":[{"award-number":["GA639249"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2025,5]]},"DOI":"10.1016\/j.fsi.2025.110202","type":"journal-article","created":{"date-parts":[[2025,2,15]],"date-time":"2025-02-15T18:52:29Z","timestamp":1739645549000},"page":"110202","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"special_numbering":"C","title":["Functional studies and synchrotron FTIR biochemical signatures reveal the potential of protein nanoparticles as a VHS virus vaccine"],"prefix":"10.1016","volume":"160","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7533-8717","authenticated-orcid":false,"given":"Rosemary","family":"Thwaite","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00faria","family":"Benseny-Cases","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2373-1792","authenticated-orcid":false,"given":"Mauricio","family":"Rojas-Pe\u00f1a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6983-2786","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Chico","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Carreras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Puente-Marin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Villaverde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4078-8763","authenticated-orcid":false,"given":"Luis","family":"Perez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2065-0601","authenticated-orcid":false,"given":"Maria del Mar","family":"Ortega-Villaizan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manel","family":"Sab\u00e9s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nerea","family":"Roher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2025.110202_bib1","series-title":"Viral Vaccines for Farmed Finfish","author":"Dhar","year":"2014"},{"key":"10.1016\/j.fsi.2025.110202_bib2","series-title":"Emerging Viruses in Aquaculture","author":"Kibenge","year":"2019"},{"issue":"2","key":"10.1016\/j.fsi.2025.110202_bib3","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.tibtech.2011.09.003","article-title":"Bacterial inclusion bodies: making gold from waste","volume":"30","author":"Garc\u00eda-Fruit\u00f3s","year":"2012","journal-title":"Trends Biotechnol."},{"issue":"9","key":"10.1016\/j.fsi.2025.110202_bib4","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.tibs.2017.01.005","article-title":"Bacterial inclusion bodies: discovering their better half","volume":"42","author":"Rinas","year":"2017","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.fsi.2025.110202_bib5","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.biomaterials.2016.08.043","article-title":"Nanostructured recombinant cytokines: a highly stable alternative to short-lived prophylactics","volume":"107","author":"Torrealba","year":"2016","journal-title":"Biomaterials"},{"key":"10.1016\/j.fsi.2025.110202_bib6","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1016\/j.fsi.2022.10.067","article-title":"How modular protein nanoparticles may expand the ability of subunit anti-viral vaccines: the spring viremia carp virus (SVCV) case","volume":"131","author":"Rojas-Pe\u00f1a","year":"2022","journal-title":"Fish Shellfish Immunol."},{"issue":"31","key":"10.1016\/j.fsi.2025.110202_bib7","doi-asserted-by":"crossref","first-page":"3955","DOI":"10.1016\/j.vaccine.2014.05.010","article-title":"Targeting and stimulation of the zebrafish (Danio rerio) innate immune system with LPS\/dsRNA-loaded nanoliposomes","volume":"32","author":"Ruyra","year":"2014","journal-title":"Vaccine"},{"issue":"Jul","key":"10.1016\/j.fsi.2025.110202_bib8","article-title":"Protein nanoparticles made of recombinant viral antigens: a promising biomaterial for oral delivery of fish prophylactics","volume":"9","author":"Thwaite","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2025.110202_bib9","doi-asserted-by":"crossref","first-page":"201","DOI":"10.3354\/dao072201","article-title":"Viral haemorrhagic septicaemia (VHS) outbreaks in Finnish rainbow trout farms","volume":"72","author":"Raja-Halli","year":"2006","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.fsi.2025.110202_bib10","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2016.00192","article-title":"Turbot (Scophthalmus maximus) vs. VHSV (viral hemorrhagic septicemia virus): a review","volume":"7","author":"Pereiro","year":"2016","journal-title":"Front. Physiol."},{"issue":"1\u20132","key":"10.1016\/j.fsi.2025.110202_bib11","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.aquaculture.2009.07.019","article-title":"An outbreak of VHSV (viral hemorrhagic septicemia virus) infection in farmed olive flounder Paralichthys olivaceus in Korea","volume":"296","author":"Kim","year":"2009","journal-title":"Aquaculture"},{"issue":"1\u20132","key":"10.1016\/j.fsi.2025.110202_bib12","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.vetmic.2012.07.020","article-title":"Viral tropism and pathology associated with viral hemorrhagic septicemia in larval and juvenile Pacific herring","volume":"161","author":"Lovy","year":"2012","journal-title":"Vet. Microbiol."},{"issue":"9","key":"10.1016\/j.fsi.2025.110202_bib13","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1111\/j.1365-2761.2005.00654.x","article-title":"Viral haemorrhagic septicaemia virus in marine fish and its implications for fish farming \u2013 a review","volume":"28","author":"Skall","year":"2005","journal-title":"J. Fish. Dis."},{"issue":"13","key":"10.1016\/j.fsi.2025.110202_bib14","doi-asserted-by":"crossref","first-page":"1938","DOI":"10.1016\/j.vaccine.2009.01.103","article-title":"The immunogenicity of viral haemorragic septicaemia rhabdovirus (VHSV) DNA vaccines can depend on plasmid regulatory sequences","volume":"27","author":"Chico","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2025.110202_bib16","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.fsi.2017.01.031","article-title":"Enhancement of glycoprotein-based DNA vaccine for viral hemorrhagic septicemia virus (VHSV) via addition of the molecular adjuvant, DDX41","volume":"62","author":"Lazarte","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110202_bib17","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.fsi.2018.07.012","article-title":"DNA vaccination for finfish aquaculture","volume":"85","author":"Collins","year":"2019","journal-title":"Fish Shellfish Immunol."},{"issue":"7","key":"10.1016\/j.fsi.2025.110202_bib18","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1016\/j.vaccine.2018.12.063","article-title":"PLGA encapsulated inactivated-viral vaccine: formulation and evaluation of its protective efficacy against viral haemorrhagic septicaemia virus (VHSV) infection in olive flounder (Paralichthys olivaceus) vaccinated by mucosal delivery routes","volume":"37","author":"Kole","year":"2019","journal-title":"Vaccine"},{"issue":"6","key":"10.1016\/j.fsi.2025.110202_bib19","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1016\/j.immuni.2016.06.006","article-title":"Type 1 interferons induce changes in core metabolism that are critical for immune function","volume":"44","author":"Wu","year":"2016","journal-title":"Immunity"},{"issue":"6","key":"10.1016\/j.fsi.2025.110202_bib20","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.it.2017.03.001","article-title":"Macrophage immunometabolism: where are we (going)?","volume":"38","author":"Van den Bossche","year":"2017","journal-title":"Trends Immunol."},{"issue":"10","key":"10.1016\/j.fsi.2025.110202_bib21","doi-asserted-by":"crossref","DOI":"10.3390\/v10100521","article-title":"Interplay between cellular metabolism and cytokine responses during viral infection","volume":"10","author":"Zhang","year":"2018","journal-title":"Viruses"},{"issue":"3","key":"10.1016\/j.fsi.2025.110202_bib22","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1016\/j.fsi.2011.01.021","article-title":"Rainbow trout surviving infections of viral haemorrhagic septicemia virus (VHSV) show lasting antibodies to recombinant G protein fragments","volume":"30","author":"Encinas","year":"2011","journal-title":"Fish Shellfish Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2025.110202_bib23","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1177\/1753425909348232","article-title":"Endotoxin recognition in fish results in inflammatory cytokine secretion not gene expression","volume":"17","author":"Roher","year":"2011","journal-title":"Innate Immun."},{"issue":"4","key":"10.1016\/j.fsi.2025.110202_bib24","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1080\/08940886.2017.1338410","article-title":"MIRAS: the infrared synchrotron radiation beamline at ALBA","volume":"30","author":"Yousef","year":"2017","journal-title":"Synchrotron Radiat. News"},{"issue":"4","key":"10.1016\/j.fsi.2025.110202_bib25","doi-asserted-by":"crossref","first-page":"2772","DOI":"10.1021\/acs.analchem.7b04818","article-title":"Synchrotron-based fourier transform infrared microspectroscopy (\u03bcFTIR) study on the effect of alzheimer's a\u03b2 amorphous and fibrillar aggregates on PC12 cells","volume":"90","author":"Benseny-Cases","year":"2018","journal-title":"Anal. Chem."},{"key":"10.1016\/j.fsi.2025.110202_bib26","doi-asserted-by":"crossref","first-page":"924","DOI":"10.3390\/biology12070924","article-title":"Immune transcriptional response in head kidney primary cell cultures isolated from the three most important species in Chilean salmonids aquaculture","volume":"12","author":"Nualart","year":"2023","journal-title":"Biology"},{"issue":"14","key":"10.1016\/j.fsi.2025.110202_bib27","doi-asserted-by":"crossref","first-page":"3451","DOI":"10.1039\/C5SM02930A","article-title":"Cellular uptake and intracellular fate of protein releasing bacterial amyloids in mammalian cells","volume":"12","author":"Seras-Franzoso","year":"2016","journal-title":"Soft Matter"},{"issue":"4","key":"10.1016\/j.fsi.2025.110202_bib28","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2025.110202_bib29","series-title":"Identification s\u00e9rologique des rhabdovirus des salmonid\u00e9s","author":"Le Berre","year":"1977"},{"issue":"8","key":"10.1016\/j.fsi.2025.110202_bib30","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1111\/j.1365-2761.2010.01165.x","article-title":"Current lineages of the epithelioma papulosum cyprini (EPC) cell line are contaminated with fathead minnow, Pimephales promelas, cells","volume":"33","author":"Winton","year":"2010","journal-title":"J. Fish. Dis."},{"issue":"9","key":"10.1016\/j.fsi.2025.110202_bib31","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.4161\/auto.29557","article-title":"Autophagy-inducing peptides from mammalian VSV and fish VHSV rhabdoviral G glycoproteins (G) as models for the development of new therapeutic molecules","volume":"10","author":"Garcia-Valtanen","year":"2014","journal-title":"Autophagy"},{"issue":"7","key":"10.1016\/j.fsi.2025.110202_bib32","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1002\/eji.1830130706","article-title":"Lymphocyte heterogeneity in the trout, Salmo gairdneri, defined with monoclonal antibodies to IgM","volume":"13","author":"Deluca","year":"1983","journal-title":"Eur. J. Immunol."},{"issue":"3","key":"10.1016\/j.fsi.2025.110202_bib33","doi-asserted-by":"crossref","first-page":"167","DOI":"10.3354\/dao034167","article-title":"Mapping of linear antibody epitopes of the glycoprotein of VHSV, a salmonid rhabdovirus","volume":"34","author":"Fernandez-Alonso","year":"1998","journal-title":"Dis. Aquat. Org."},{"issue":"1\u20132","key":"10.1016\/j.fsi.2025.110202_bib34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0166-0934(95)01972-3","article-title":"Fast neutralization\/immunoperoxidase assay for viral haemorrhagic septicaemia with anti-nucleoprotein monoclonal antibody","volume":"58","author":"Lorenzo","year":"1996","journal-title":"J. Virol Methods"},{"issue":"1","key":"10.1016\/j.fsi.2025.110202_bib35","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/j.1365-2761.1993.tb00847.x","article-title":"Monoclonal antibodies against the structural proteins of viral haemorrhagic septicaemia virus isolates","volume":"16","author":"Sanz","year":"1993","journal-title":"J. Fish. Dis."},{"issue":"7","key":"10.1016\/j.fsi.2025.110202_bib36","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"issue":"2","key":"10.1016\/j.fsi.2025.110202_bib38","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.cytogfr.2011.02.001","article-title":"Toll-like receptor, RIG-I-like receptors and the NLRP3 inflammasome: key modulators of innate immune responses to double-stranded RNA viruses","volume":"22","author":"Yu","year":"2011","journal-title":"Cytokine Growth Factor Rev."},{"issue":"5","key":"10.1016\/j.fsi.2025.110202_bib39","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1016\/j.immuni.2011.05.006","article-title":"Toll-like receptors and their crosstalk with other innate receptors in infection and immunity","volume":"34","author":"Kawai","year":"2011","journal-title":"Immunity"},{"key":"10.1016\/j.fsi.2025.110202_bib40","article-title":"Lipid peroxidation by-products and the metabolic syndrome","author":"Pillon","year":"2012","journal-title":"Lipid Peroxidation"},{"issue":"14","key":"10.1016\/j.fsi.2025.110202_bib41","doi-asserted-by":"crossref","first-page":"7140","DOI":"10.1128\/JVI.00023-10","article-title":"Pepscan mapping of viral hemorrhagic septicemia virus glycoprotein G major lineal determinants implicated in triggering host cell antiviral responses mediated by type I interferon","volume":"84","author":"Chico","year":"2010","journal-title":"J. Virol."},{"issue":"7","key":"10.1016\/j.fsi.2025.110202_bib42","doi-asserted-by":"crossref","DOI":"10.1002\/adma.201907348","article-title":"Engineering secretory amyloids for remote and highly selective destruction of metastatic foci","volume":"32","author":"C\u00e9spedes","year":"2020","journal-title":"Adv. Mater."},{"key":"10.1016\/j.fsi.2025.110202_bib43","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1016\/j.fsi.2020.02.029","article-title":"Nanostructured recombinant protein particles raise specific antibodies against the nodavirus NNV coat protein in sole","volume":"99","author":"Thwaite","year":"2020","journal-title":"Fish Shellfish Immunol."},{"issue":"7","key":"10.1016\/j.fsi.2025.110202_bib44","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/0923-2494(89)90021-7","article-title":"C. Torchy, P. De Kinkelin, and S. Avrameas, \u201cNatural anti-TNP antibodies from rainbow trout interfere with viral infection in vitro,\u201d","volume":"140","author":"Gonzalez","year":"1989","journal-title":"Res. Immunol."},{"issue":"1\u20132","key":"10.1016\/j.fsi.2025.110202_bib45","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/0014-5793(90)80535-Q","article-title":"A semi-empirical method for prediction of antigenic determinants on protein antigens","volume":"276","author":"Kolaskar","year":"1990","journal-title":"FEBS Lett."},{"issue":"6","key":"10.1016\/j.fsi.2025.110202_bib46","doi-asserted-by":"crossref","first-page":"1289","DOI":"10.1016\/j.vaccine.2010.11.073","article-title":"The introduction of multi-copy CpG motifs into an antiviral DNA vaccine strongly up-regulates its immunogenicity in fish","volume":"29","author":"Martinez-Alonso","year":"2011","journal-title":"Vaccine"}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464825000919?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464825000919?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T15:04:08Z","timestamp":1762355048000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464825000919"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":44,"alternative-id":["S1050464825000919"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2025.110202","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Functional studies and synchrotron FTIR biochemical signatures reveal the potential of protein nanoparticles as a VHS virus vaccine","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2025.110202","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"110202"}}