{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T13:46:10Z","timestamp":1768830370450,"version":"3.49.0"},"reference-count":71,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004195","name":"Ocean University of China","doi-asserted-by":"publisher","award":["2B7"],"award-info":[{"award-number":["2B7"]}],"id":[{"id":"10.13039\/501100004195","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004195","name":"Ocean University of China","doi-asserted-by":"publisher","award":["3A6"],"award-info":[{"award-number":["3A6"]}],"id":[{"id":"10.13039\/501100004195","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015956","name":"Special Project for Research and Development in Key Areas of Guangdong Province","doi-asserted-by":"publisher","award":["2021B0202040002"],"award-info":[{"award-number":["2021B0202040002"]}],"id":[{"id":"10.13039\/501100015956","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015956","name":"Special Project for Research and Development in Key Areas of Guangdong Province","doi-asserted-by":"publisher","award":["311021006"],"award-info":[{"award-number":["311021006"]}],"id":[{"id":"10.13039\/501100015956","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100031919","name":"Southern Marine Science and Engineering Guangdong Laboratory","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100031919","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018914","name":"Fujian Academy of Agricultural Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018914","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2025,7]]},"DOI":"10.1016\/j.fsi.2025.110358","type":"journal-article","created":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T18:58:27Z","timestamp":1745002707000},"page":"110358","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Dual protection against grouper Rana-Iridovirus (GIV-R) and nervous necrosis virus (NNV) by novel GIV-R-\u039451\u2212NNV-CP chimeric vaccine candidates"],"prefix":"10.1016","volume":"162","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8159-2916","authenticated-orcid":false,"given":"Shan","family":"Ding","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianqian","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiming","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fangzhao","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaoping","family":"Weng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianguo","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6276-7993","authenticated-orcid":false,"given":"Chuanfu","family":"Dong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2025.110358_bib1","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.3390\/v2071458","article-title":"The genomic diversity and phylogenetic relationship in the family iridoviridae","volume":"2","author":"Eaton","year":"2010","journal-title":"Viruses"},{"key":"10.1016\/j.fsi.2025.110358_bib2","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1099\/jgv.0.000818","article-title":"ICTV virus taxonomy profile: iridoviridae","volume":"98","author":"Chinchar","year":"2017","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib3","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.virol.2005.05.021","article-title":"Complete genome sequence analysis of an iridovirus isolated from the orange-spotted grouper, Epinephelus coioides","volume":"339","author":"L\u00fc","year":"2005","journal-title":"Virology"},{"key":"10.1016\/j.fsi.2025.110358_bib4","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1111\/j.1365-2761.1994.tb00237.x","article-title":"Investigation of outbreaks of a novel disease, 'sleepy grouper disease', affecting the brown-spotted grouper, Epinephelus tauvina Forskal","volume":"17","author":"Chua","year":"1994","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2025.110358_bib5","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1111\/jfd.12244","article-title":"Identification and characterization of a novel lymphocystis disease virus isolate from cultured grouper in China","volume":"38","author":"Huang","year":"2015","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2025.110358_bib6","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1046\/j.1365-2761.2000.00247.x","article-title":"Two iridovirus-susceptible cell lines established from kidney and liver of grouper, Epinephelus awoara (Temminck & Schlegel), and partial characterization of grouper iridovirus","volume":"23","author":"Lai","year":"2000","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2025.110358_bib7","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1046\/j.1365-2761.2002.00343.x","article-title":"Molecular characterization and pathogenicity of a grouper iridovirus (GIV) isolated from yellow grouper, Epinephelus awoara (Temminck &Schlegel)","volume":"25","author":"Murali","year":"2002","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2025.110358_bib8","doi-asserted-by":"crossref","first-page":"12576","DOI":"10.1128\/JVI.78.22.12576-12590.2004","article-title":"Functional genomics analysis of Singapore grouper iridovirus: complete sequence determination and proteomic analysis","volume":"78","author":"Song","year":"2004","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib9","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.aquaculture.2016.05.032","article-title":"Isolation of a Ranavirus-type grouper iridovirus in mainland China and comparison of its pathogenicity with that of a Megalocytivirus-type grouper iridovirus","volume":"463","author":"Ma","year":"2016","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110358_bib10","doi-asserted-by":"crossref","first-page":"521","DOI":"10.3390\/v4040521","article-title":"Review: megalocytiviruses","volume":"4","author":"Kurita","year":"2012","journal-title":"Viruses"},{"key":"10.1016\/j.fsi.2025.110358_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2020.736326","article-title":"Pathogenicity and histopathology of infectious spleen and kidney necrosis virus genotype II (ISKNV-II) recovering from mass mortality of farmed Asian seabass, Lates calcarifer, in Southern China","volume":"534","author":"Zhu","year":"2021","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110358_bib12","doi-asserted-by":"crossref","first-page":"45","DOI":"10.3147\/jsfp.37.45","article-title":"Cultured fish species affected by red sea bream iridoviral disease from 1996 to 2000","volume":"37","author":"Kawakami","year":"2002","journal-title":"Fish Pathol."},{"key":"10.1016\/j.fsi.2025.110358_bib13","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1007\/s00705-006-0870-4","article-title":"Molecular epidemiology and phylogenetic analysis of a marine fish infectious spleen and kidney necrosis virus-like (ISKNV-like) virus","volume":"152","author":"Wang","year":"2007","journal-title":"Arch. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib14","doi-asserted-by":"crossref","first-page":"2010","DOI":"10.1128\/JVI.79.4.2010-2023.2005","article-title":"Complete genome sequence of the grouper iridovirus and comparison of genomic organization with those of other iridoviruses","volume":"79","author":"Tsai","year":"2005","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib15","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1007\/s00705-011-1017-9","article-title":"Genetic analysis of fish iridoviruses isolated in Taiwan during 2001-2009","volume":"156","author":"Huang","year":"2011","journal-title":"Arch. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3354\/dao053001","article-title":"Characterization of a novel ranavirus isolated from grouper Epinephelus tauvina","volume":"53","author":"Qin","year":"2003","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.fsi.2025.110358_bib17","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1002\/aah.10090","article-title":"Isolation and characterization of a ranavirus associated with disease outbreaks in cultured hybrid grouper (female symbol tiger grouper Epinephelus fuscoguttatus x male symbol giant grouper E. lanceolatus) in Guangxi, China","volume":"31","author":"Xiao","year":"2019","journal-title":"J. Aquat. Anim. Health"},{"key":"10.1016\/j.fsi.2025.110358_bib18","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0166-0934(01)00350-0","article-title":"Electron microscopic observations of a marine fish iridovirus isolated from brown-spotted grouper, Epinephelus tauvina","volume":"98","author":"Qin","year":"2001","journal-title":"J. Virol Methods"},{"key":"10.1016\/j.fsi.2025.110358_bib19","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1046\/j.1365-2761.2003.00434.x","article-title":"Establishment of cell lines from a tropical grouper, Epinephelus awoara (Temminck & Schlegel), and their susceptibility to grouper irido- and nodaviruses","volume":"26","author":"Lai","year":"2003","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2025.110358_bib20","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.fsi.2015.12.041","article-title":"Differential autophagic effects triggered by five different vertebrate iridoviruses in a common, highly permissive mandarinfish fry (MFF-1) cell model","volume":"49","author":"Qi","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110358_bib21","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.vetmic.2012.10.026","article-title":"Efficacy of a formalin-killed cell vaccine against infectious spleen and kidney necrosis virus (ISKNV) and immunoproteomic analysis of its major immunogenic proteins","volume":"162","author":"Dong","year":"2013","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.fsi.2025.110358_bib22","doi-asserted-by":"crossref","first-page":"1598","DOI":"10.1016\/j.fsi.2013.09.005","article-title":"Field trial tests of FKC vaccines against RSIV genotype Megalocytivirus in cage-cultured Mandarin fish (Siniperca chuatsi) in an inland reservoir","volume":"35","author":"Dong","year":"2013","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110358_bib23","first-page":"205","volume":"vol. 32","author":"Nakajima","year":"1997"},{"key":"10.1016\/j.fsi.2025.110358_bib24","doi-asserted-by":"crossref","first-page":"163","DOI":"10.3354\/dao01896","article-title":"Histopathological and ultrastructural features of enlarged cells of humpback grouper Cromileptes altivelis challenged with Megalocytivirus (family Iridoviridae) after vaccination","volume":"79","author":"Mahardika","year":"2008","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.fsi.2025.110358_bib25","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.dci.2012.07.004","article-title":"Immunogenicity and protective effects of inactivated Singapore grouper iridovirus (SGIV) vaccines in orange-spotted grouper, Epinephelus coioides","volume":"38","author":"Ou-yang","year":"2012","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2025.110358_bib26","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2022.738455","article-title":"Effective protection against Ranavirus-type grouper iridovirus and Vibrio harveyi by priming a bivalent GrouperVAC-Irido-R-Vh followed by boosting a monovalent V. harveyi vaccine","volume":"560","author":"Ma","year":"2022","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110358_bib27","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.109218","article-title":"Development and immune evaluation of LAMP1 chimeric DNA vaccine against Singapore grouper iridovirus in orange-spotted grouper, Epinephelus coioides","volume":"144","author":"Xu","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110358_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.109855","article-title":"Evaluation of protective immune response of immersion inactivated vaccine against Singapore grouper iridovirus","volume":"153","author":"Xu","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110358_bib29","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens9020106","article-title":"Betanodavirus and ver disease: a 30-year research review","volume":"9","author":"Band\u00edn","year":"2020","journal-title":"Pathogens"},{"key":"10.1016\/j.fsi.2025.110358_bib30","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1046\/j.1365-2761.2002.00350.x","article-title":"Betanodavirus infections of teleost fish: a review","volume":"25","author":"Munday","year":"2002","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2025.110358_bib31","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1128\/aem.63.4.1633-1636.1997","article-title":"Genomic classification of fish nodaviruses by molecular phylogenetic analysis of the coat protein gene","volume":"63","author":"Nishizawa","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.fsi.2025.110358_bib32","doi-asserted-by":"crossref","first-page":"2979","DOI":"10.1007\/s00705-015-2590-0","article-title":"Epidemiological characterization of VNNV in hatchery-reared and wild marine fish on Hainan Island, China, and experimental infection of golden pompano (Trachinotus ovatus) juveniles","volume":"160","author":"Ma","year":"2015","journal-title":"Arch. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib33","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.fsi.2019.09.031","article-title":"Current status of fish vaccines in Japan","volume":"95","author":"Matsuura","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110358_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2019.734211","article-title":"Propagations of grouper (Epinephelus sp.) viruses in a new fibroblast-like cell line from orange spotted grouper (E. coioides) brain","volume":"511","author":"Ma","year":"2019","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110358_bib35","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0036183","article-title":"Nervous necrosis virus replicates following the embryo development and dual infection with iridovirus at juvenile stage in grouper","volume":"7","author":"Kuo","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2025.110358_bib36","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.aquaculture.2012.06.032","article-title":"Co-infection of megalocytivirus and viral nervous necrosis virus in a very severe mass mortality of juvenile orange-spotted groupers (Epinephelus coioides)","volume":"358\u2013359","author":"Ma","year":"2012","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110358_bib37","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2024.741179","article-title":"Immune responses in giant groupers after vaccination with inactivated bivalent vaccine against betanodavirus and ranavirus","volume":"592","author":"Kai","year":"2024","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110358_bib38","doi-asserted-by":"crossref","DOI":"10.1002\/eji.202250133","article-title":"YF17D-based vaccines - standing on the shoulders of a giant","volume":"54","author":"Sanchez-Felipe","year":"2024","journal-title":"Eur. J. Immunol."},{"key":"10.1016\/j.fsi.2025.110358_bib39","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1138961","article-title":"Recombinant viral hemorrhagic septicemia virus with rearranged genomes as vaccine vectors to protect against lethal betanodavirus infection","volume":"14","author":"Souto","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2025.110358_bib40","article-title":"Generation and characterization of ORF55\/ORF57-deleted recombinant Cyprinid herpesvirus 2 mutants with chimeric capsid protein gene of grouper nervous necrosis virus","volume":"12","author":"Feng","year":"2024","journal-title":"Vaccines"},{"key":"10.1016\/j.fsi.2025.110358_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2022.738562","article-title":"Development and characterization of monoclonal antibodies against red-spotted grouper nervous necrosis virus and their neutralizing potency","volume":"560","author":"Zhang","year":"2022","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110358_bib42","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.vetimm.2013.10.003","article-title":"Immune responses of orange-spotted grouper, Epinephelus coioides, against virus-like particles of betanodavirus produced in Escherichia coli","volume":"157","author":"Lai","year":"2014","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.fsi.2025.110358_bib43","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2022.739177","article-title":"Immune and protective effects of subunit vaccines from S-domain or P-domain in capsid protein against nervous necrosis virus in pearl gentian grouper","volume":"566","author":"Lin","year":"2023","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110358_bib44","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.virusres.2008.04.004","article-title":"Development of a Mandarin fish Siniperca chuatsi fry cell line suitable for the study of infectious spleen and kidney necrosis virus (ISKNV)","volume":"135","author":"Dong","year":"2008","journal-title":"Virus Res."},{"key":"10.1016\/j.fsi.2025.110358_bib45","doi-asserted-by":"crossref","DOI":"10.1111\/jfd.14052","article-title":"The Co-infection of ISKNV-II and RGNNV resulting in mass mortality of juvenile asian seabass (Lates calcarifer), Zhuhai, southern China","volume":"48","author":"Sun","year":"2025","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2025.110358_bib46","doi-asserted-by":"crossref","first-page":"2537","DOI":"10.1099\/0022-1317-72-10-2537","article-title":"Spontaneously productive C-type retrovirus infection of fish cell lines","volume":"72","author":"Frerichs","year":"1991","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib47","doi-asserted-by":"crossref","first-page":"2869","DOI":"10.1128\/JVI.01444-10","article-title":"Global landscape of structural proteins of infectious spleen and kidney necrosis virus","volume":"85","author":"Dong","year":"2011","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib48","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.04495-22","article-title":"The inactivated ISKNV-I vaccine confers highly effective cross-protection against epidemic RSIV-I and RSIV-II from cultured spotted sea bass Lateolabrax maculatus","volume":"11","author":"Fu","year":"2023","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.fsi.2025.110358_bib49","first-page":"493","article-title":"A simple method of estimating fifty percent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Hyg."},{"key":"10.1016\/j.fsi.2025.110358_bib50","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.jviromet.2007.08.016","article-title":"Bohle iridovirus as a vector for heterologous gene expression","volume":"146","author":"Pallister","year":"2007","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.fsi.2025.110358_bib51","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.virusres.2011.08.012","article-title":"Rana grylio virus as a vector for foreign gene expression in fish cells","volume":"163","author":"He","year":"2012","journal-title":"Virus Res."},{"key":"10.1016\/j.fsi.2025.110358_bib52","doi-asserted-by":"crossref","first-page":"5061","DOI":"10.1128\/JVI.01488-10","article-title":"Innate immune evasion mediated by the Ambystoma tigrinum virus eukaryotic translation initiation factor 2alpha homologue","volume":"85","author":"Jancovich","year":"2011","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib53","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.fsi.2022.02.041","article-title":"Deleting ORF71L of infectious spleen and kidney necrosis virus (ISKNV) resulted in virulence attenuation in Mandarin fish","volume":"123","author":"Zhang","year":"2022","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110358_bib54","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.virol.2017.06.025","article-title":"Singapore grouper iridovirus (SGIV) TNFR homolog VP51 functions as a virulence factor via modulating host inflammation response","volume":"511","author":"Yu","year":"2017","journal-title":"Virology"},{"key":"10.1016\/j.fsi.2025.110358_bib55","article-title":"Singapore grouper iridovirus protein VP088 is essential for viral infectivity","volume":"6","author":"Yuan","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fsi.2025.110358_bib56","article-title":"Low-temperature immunization attenuates the residual virulence of orf074r gene-deleted infectious spleen and kidney necrosis virus: a candidate immersion vaccine","volume":"97","author":"Zeng","year":"2023","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib57","article-title":"A safe and efficient double-gene-deleted live attenuated immersion vaccine to prevent the disease caused by the infectious spleen and kidney necrosis virus","volume":"97","author":"Zeng","year":"2023","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib58","doi-asserted-by":"crossref","first-page":"3534","DOI":"10.1128\/JVI.76.7.3534-3543.2002","article-title":"Capsid protein C of tick-borne encephalitis virus tolerates large internal deletions and is a favorable target for attenuation of virulence","volume":"76","author":"Kofler","year":"2002","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib59","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1128\/JVI.01467-06","article-title":"A severe acute respiratory syndrome coronavirus that lacks the E gene is attenuated in vitro and in vivo","volume":"81","author":"DeDiego","year":"2007","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib60","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1016\/j.cell.2020.07.012","article-title":"The impact of mutations in SARS-CoV-2 spike on viral infectivity and antigenicity","volume":"182","author":"Li","year":"2020","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2025.110358_bib61","doi-asserted-by":"crossref","first-page":"2236","DOI":"10.1002\/pmic.200900820","article-title":"Proteomic analysis of Singapore grouper iridovirus envelope proteins and characterization of a novel envelope protein VP088","volume":"11","author":"Zhou","year":"2011","journal-title":"Proteomics"},{"key":"10.1016\/j.fsi.2025.110358_bib62","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.00686-24","article-title":"Nervous necrosis virus capsid protein and Protein A dynamically modulate the fish cGAS-mediated IFN signal pathway to facilitate viral evasion","author":"Huang","year":"2024","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib63","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2023.739428","article-title":"A mandarinfish Siniperca chuatsi infection and vaccination model for SDDV and efficacy evaluation of the formalin-killed cell vaccine in yellowfin seabream Acanthopagrus latus","volume":"570","author":"Fu","year":"2023","journal-title":"Acquaculture"},{"key":"10.1016\/j.fsi.2025.110358_bib64","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1005074","article-title":"A novel virus causes scale drop disease in Lates calcarifer","volume":"11","author":"de Groof","year":"2015","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.fsi.2025.110358_bib65","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2022.739218","article-title":"Effectively protecting Asian seabass Lates calcarifer from ISKNV-I, ISKNV-II, RSIV-II and SDDV by an inactivated ISKNV-I and SDDV bivalent vaccine","volume":"566","author":"Fu","year":"2023","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110358_bib66","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.vetmic.2017.04.022","article-title":"Oral vaccination through voluntary consumption of the convict grouper Epinephelus septemfasciatus with yeast producing the capsid protein of red-spotted grouper nervous necrosis virus","volume":"204","author":"Cho","year":"2017","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.fsi.2025.110358_bib67","doi-asserted-by":"crossref","first-page":"880","DOI":"10.3389\/fpls.2019.00880","article-title":"Plant-made nervous necrosis virus-like particles protect fish against disease","volume":"10","author":"Marsian","year":"2019","journal-title":"Front. Plant Sci."},{"key":"10.1016\/j.fsi.2025.110358_bib68","doi-asserted-by":"crossref","first-page":"10201","DOI":"10.1128\/JVI.01098-06","article-title":"Induction of a protective immune response against viral nervous necrosis in the European sea bass Dicentrarchus labrax by using betanodavirus virus-like particles","volume":"80","author":"Thiery","year":"2006","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110358_bib69","doi-asserted-by":"crossref","first-page":"15373","DOI":"10.1021\/ja403550f","article-title":"Norovirus capsid proteins self-assemble through biphasic kinetics via long-lived stave-like intermediates","volume":"135","author":"Tresset","year":"2013","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.fsi.2025.110358_bib70","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13567-015-0294-9","article-title":"Structural analysis and insertion study reveal the ideal sites for surface displaying foreign peptides on a betanodavirus-like particle","volume":"47","author":"Xie","year":"2016","journal-title":"Vet. Res."},{"key":"10.1016\/j.fsi.2025.110358_bib71","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1005203","article-title":"Crystal structures of a piscine betanodavirus: mechanisms of capsid assembly and viral infection","volume":"11","author":"Chen","year":"2015","journal-title":"PLoS Pathog."}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464825002475?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464825002475?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T15:05:20Z","timestamp":1762355120000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464825002475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":71,"alternative-id":["S1050464825002475"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2025.110358","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Dual protection against grouper Rana-Iridovirus (GIV-R) and nervous necrosis virus (NNV) by novel GIV-R-\u039451\u2212NNV-CP chimeric vaccine candidates","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2025.110358","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"110358"}}