{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T12:09:07Z","timestamp":1778587747326,"version":"3.51.4"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:00:00Z","timestamp":1746662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["22\\u201303236"],"award-info":[{"award-number":["22\\u201303236"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002805","name":"Carl Tryggers Stiftelse f\u00f6r Vetenskaplig Forskning","doi-asserted-by":"publisher","award":["2022"],"award-info":[{"award-number":["2022"]}],"id":[{"id":"10.13039\/501100002805","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002805","name":"Carl Tryggers Stiftelse f\u00f6r Vetenskaplig Forskning","doi-asserted-by":"publisher","award":["CTS 22\\u20132021"],"award-info":[{"award-number":["CTS 22\\u20132021"]}],"id":[{"id":"10.13039\/501100002805","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004047","name":"Karolinska Institutet","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["PID2020-116115 GB-100"],"award-info":[{"award-number":["PID2020-116115 GB-100"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["202310500004"],"award-info":[{"award-number":["202310500004"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Spain Ministry of Science and Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2025,8]]},"DOI":"10.1016\/j.fsi.2025.110392","type":"journal-article","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T02:31:07Z","timestamp":1746757867000},"page":"110392","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Specific host factors determine resistance in a North American crayfish to the crayfish plague, Aphanomyces astaci"],"prefix":"10.1016","volume":"163","author":[{"given":"Yanhong","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlotta","family":"Ekblom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3191-5564","authenticated-orcid":false,"given":"Thanapong","family":"Kruangkum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4488-0658","authenticated-orcid":false,"given":"Javier Dieguez","family":"Uribeondo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7029-5946","authenticated-orcid":false,"given":"Kenneth","family":"S\u00f6derh\u00e4ll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3671-2689","authenticated-orcid":false,"given":"Irene","family":"S\u00f6derh\u00e4ll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2025.110392_bib1","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1111\/j.1365-2109.1993.tb00655.x","article-title":"Procambarus clarkii Girard as a vector for the crayfish plague fungus, Aphanomyces astaci Schikora","volume":"24","author":"Di\u00e9guez-Uribeondo","year":"1993","journal-title":"Aquac. Res."},{"key":"10.1016\/j.fsi.2025.110392_bib2","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1111\/j.1365-2761.1987.tb01098.x","article-title":"The influence of haemocyte number on the resistance of the freshwater crayfish, Pacifastacus leniusculus Dana, to the parasitic fungus Aphanomyces astaci","volume":"10","author":"Persson","year":"1987","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2025.110392_bib3","unstructured":"S\u00f6derh\u00e4ll K, Cerenius L., The Crayfish Plague Fungus: History and Recent Advances, Freshwater Crayfish 1998 (n.d.) 11\u201335."},{"key":"10.1016\/j.fsi.2025.110392_bib4","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1111\/j.1399-3054.1969.tb07372.x","article-title":"On the physiology of zoospore production in Aphanomyces astaci","volume":"22","author":"Unestam","year":"1969","journal-title":"Physiol. Plantarum"},{"key":"10.1016\/j.fsi.2025.110392_bib5","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/0147-5975(85)90022-2","article-title":"Repeated zoospore emergence as a possible adaptation to parasitism in Aphanomyces","volume":"9","author":"Cerenius","year":"1985","journal-title":"Exp. Mycol."},{"key":"10.1016\/j.fsi.2025.110392_bib6","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/0022-2011(75)90233-5","article-title":"Ultrastructure of the penetration of the crayfish integument by the fungal parasite, Aphanomyces astaci, Oomycetes","volume":"26","author":"Nyhl\u00e9n","year":"1975","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.fsi.2025.110392_bib7","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1111\/j.1399-3054.1975.tb03883.x","article-title":"Properties of extracellular enzymes from Aphanomyces astaci and their relevance in the penetration process of crayfish cuticle","volume":"35","author":"S\u00f6derh\u00e4ll","year":"1975","journal-title":"Physiol. Plantarum"},{"key":"10.1016\/j.fsi.2025.110392_bib8","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1007\/BF00443081","article-title":"Chitinase and protease activities in germinating zoospore cysts of a parasitic fungus, Aphanomyces astaci, Oomycetes","volume":"64","author":"S\u00f6derh\u00e4ll","year":"1978","journal-title":"Mycopathologia"},{"key":"10.1016\/j.fsi.2025.110392_bib9","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0181226","article-title":"Resistance to the crayfish plague, Aphanomyces astaci (Oomycota) in the endangered freshwater crayfish species, Austropotamobius pallipes","volume":"12","author":"Mart\u00edn-Torrijos","year":"2017","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2025.110392_bib10","doi-asserted-by":"crossref","first-page":"342","DOI":"10.3390\/jof8040342","article-title":"Resistance to crayfish plague: assessing the response of native iberian populations of the white-clawed freshwater crayfish","volume":"8","author":"Mart\u00ednez-R\u00edos","year":"2022","journal-title":"J Fungi (Basel)"},{"key":"10.1016\/j.fsi.2025.110392_bib11","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.it.2008.02.009","article-title":"The proPO-system: pros and cons for its role in invertebrate immunity","volume":"29","author":"Cerenius","year":"2008","journal-title":"Trends Immunol."},{"key":"10.1016\/j.fsi.2025.110392_bib12","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.tibs.2010.04.006","article-title":"Proteolytic cascades and their involvement in invertebrate immunity","volume":"35","author":"Cerenius","year":"2010","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.fsi.2025.110392_bib13","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1016\/j.celrep.2019.03.101","article-title":"More than black or white: melanization and Toll share regulatory serine proteases in Drosophila","volume":"27","author":"Dudzic","year":"2019","journal-title":"Cell Rep."},{"key":"10.1016\/j.fsi.2025.110392_bib14","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1111\/j.0105-2896.2004.0121.x","article-title":"Innate immune responses of a lepidopteran insect, Manduca sexta","volume":"198","author":"Kanost","year":"2004","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.fsi.2025.110392_bib15","doi-asserted-by":"crossref","first-page":"25316","DOI":"10.1074\/jbc.M804364200","article-title":"Molecular control of phenoloxidase-induced melanin synthesis in an insect","volume":"283","author":"Kan","year":"2008","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fsi.2025.110392_bib16","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2022.104850","article-title":"Characterization of hemocytes and hematopoietic cells of a freshwater crayfish based on single-cell transcriptome analysis","author":"S\u00f6derh\u00e4ll","year":"2022","journal-title":"iScience"},{"key":"10.1016\/j.fsi.2025.110392_bib17","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.dci.2018.11.016","article-title":"A comparative global proteomic analysis of the hematopoietic lineages in the crustacean Pacifastacus leniusculus","volume":"92","author":"S\u00f6derh\u00e4ll","year":"2019","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2025.110392_bib18","doi-asserted-by":"crossref","DOI":"10.1007\/s00441-024-03943-1","article-title":"The hematopoietic tissue of the freshwater crayfish, Pacifastacus leniusculus: organization and expression analysis","author":"Kruangkum","year":"2025","journal-title":"Cell Tissue Res."},{"key":"10.1016\/j.fsi.2025.110392_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2021.104098","article-title":"Immune properties of invertebrate phenoloxidases","volume":"122","author":"Cerenius","year":"2021","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2025.110392_bib20","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1016\/j.fsi.2022.10.008","article-title":"Blood cell formation in crustaceans","volume":"131","author":"S\u00f6derh\u00e4ll","year":"2022","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110392_bib21","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1111\/j.1399-3054.1965.tb06911.x","article-title":"Studies on the crayfish plague fungus Aphanomyces astaci I. Some factors affecting growth in vitro","volume":"18","author":"Unestam","year":"1965","journal-title":"Physiol. Plantarum"},{"key":"10.1016\/j.fsi.2025.110392_bib22","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jip.2009.02.002","article-title":"A highly virulent pathogen, Aeromonas hydrophila, from the freshwater crayfish Pacifastacus leniusculus","volume":"101","author":"Jiravanichpaisal","year":"2009","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.fsi.2025.110392_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.109769","article-title":"An i-type lysozyme from a crustacean, Pacifastacus leniusculus, functions as a clot-destabilising enzyme","volume":"152","author":"Ekblom","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110392_bib24","doi-asserted-by":"crossref","first-page":"33593","DOI":"10.1074\/jbc.M706113200","article-title":"Phenoloxidase is an important component of the defense against Aeromonas hydrophila Infection in a crustacean, Pacifastacus leniusculus","volume":"282","author":"Liu","year":"2007","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fsi.2025.110392_bib25","doi-asserted-by":"crossref","first-page":"1730","DOI":"10.1021\/acs.jproteome.7b00913","article-title":"Extending the compatibility of the SP3 paramagnetic bead processing approach for proteomics","volume":"17","author":"Moggridge","year":"2018","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.fsi.2025.110392_bib26","unstructured":"Schr\u00f6dinger, The PyMOL Molecular Graphics System, Version 3.0, (n.d.)."},{"key":"10.1016\/j.fsi.2025.110392_bib27","doi-asserted-by":"crossref","first-page":"6464","DOI":"10.3390\/ijms22126464","article-title":"Early changes in crayfish hemocyte proteins after injection with a \u03b2-1,3-glucan, compared to saline injected and naive animals","volume":"22","author":"Ekblom","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fsi.2025.110392_bib28","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1016\/j.dci.2005.03.010","article-title":"Exocytosis and proteomic analysis of the vesicle content of granular hemocytes from a crayfish","volume":"29","author":"Sricharoen","year":"2005","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2025.110392_bib29","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.dci.2009.08.005","article-title":"High sequence variability among hemocyte-specific Kazal-type proteinase inhibitors in decapod crustaceans","volume":"34","author":"Cerenius","year":"2010","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2025.110392_bib30","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1016\/j.fsi.2010.07.001","article-title":"Proteinase inhibitory activities of two two-domain Kazal proteinase inhibitors from the freshwater crayfish Pacifastacus leniusculus and the importance of the P(2) position in proteinase inhibitory activity","volume":"29","author":"Donpudsa","year":"2010","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110392_bib31","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-16167-x","article-title":"Prokaryotic soluble expression and purification of bioactive human fibroblast growth factor 21 using maltose-binding protein","volume":"7","author":"Nguyen","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fsi.2025.110392_bib32","doi-asserted-by":"crossref","first-page":"517","DOI":"10.3390\/toxins11090517","article-title":"Serrulin: a glycine-rich bioactive peptide from the hemolymph of the yellow Tityus serrulatus scorpion","volume":"11","author":"de Jesus Oliveira","year":"2019","journal-title":"Toxins"},{"key":"10.1016\/j.fsi.2025.110392_bib33","doi-asserted-by":"crossref","first-page":"2787","DOI":"10.1007\/s00018-010-0364-0","article-title":"Ctenidins: antimicrobial glycine-rich peptides from the hemocytes of the spider Cupiennius salei","volume":"67","author":"Baumann","year":"2010","journal-title":"Cell. Mol. Life Sci."},{"key":"10.1016\/j.fsi.2025.110392_bib34","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1016\/S0145-305X(03)00058-2","article-title":"Acanthoscurrin: a novel glycine-rich antimicrobial peptide constitutively expressed in the hemocytes of the spider Acanthoscurria gomesiana","volume":"27","author":"Lorenzini","year":"2003","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2025.110392_bib35","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2016.01484","article-title":"A glycine-rich peptide from the terrestrial Crustacean Armadillidium vulgare, displays an unexpected wide antimicrobial spectrum with membranolytic activity","volume":"7","author":"Verdon","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.fsi.2025.110392_bib36","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.fsi.2020.08.009","article-title":"Characterization and antimicrobial evaluation of a new Spgly-AMP, glycine-rich antimicrobial peptide from the mud crab Scylla paramamosain","volume":"106","author":"Xie","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110392_bib37","doi-asserted-by":"crossref","first-page":"47070","DOI":"10.1074\/jbc.M103817200","article-title":"Crustacean immunity. Antifungal peptides are generated from the C terminus of shrimp hemocyanin in response to microbial challenge","volume":"276","author":"Destoumieux-Garz\u00f3n","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fsi.2025.110392_bib38","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2025.110392_bib39","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0022-2011(79)90075-2","article-title":"Attachment of phenoloxidase to fungal cell walls in arthropod immunity","volume":"34","author":"S\u00f6derh\u00e4ll","year":"1979","journal-title":"J. Invertebr. Pathol."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464825002815?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464825002815?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T11:12:54Z","timestamp":1778584374000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464825002815"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":39,"alternative-id":["S1050464825002815"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2025.110392","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Specific host factors determine resistance in a North American crayfish to the crayfish plague, Aphanomyces astaci","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2025.110392","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"110392"}}