{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T19:27:38Z","timestamp":1791833258868,"version":"4.3.4"},"reference-count":43,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100017577","name":"Zhejiang Province Basic Public Welfare Research Program","doi-asserted-by":"publisher","award":["LY22C010003"],"award-info":[{"award-number":["LY22C010003"]}],"id":[{"id":"10.13039\/501100017577","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008043","name":"Zhejiang Sci-Tech University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008043","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270198"],"award-info":[{"award-number":["32270198"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1016\/j.fsi.2025.110568","type":"journal-article","created":{"date-parts":[[2025,7,16]],"date-time":"2025-07-16T05:14:09Z","timestamp":1752642849000},"page":"110568","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":4,"special_numbering":"C","title":["Development of a novel live attenuated vaccine based on epitope-fused OmpU1-LptDEP antigen for vibriosis control in aquaculture"],"prefix":"10.1016","volume":"165","author":[{"given":"Qian","family":"Jin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanglin","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiming","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5805-2888","authenticated-orcid":false,"given":"Jianyi","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2025.110568_bib1","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1128\/CMR.00059-09","article-title":"Epidemiology of seafood-associated infections in the United States","volume":"23","author":"Iwamoto","year":"2010","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.fsi.2025.110568_bib2","first-page":"815","article-title":"Prevalence and antibiotic resistance of pathogenic vibrios in shellfishes from cochin market","volume":"43","author":"Sudha","year":"2014","journal-title":"Indian J. Geo-Mar. Sci."},{"key":"10.1016\/j.fsi.2025.110568_bib3","doi-asserted-by":"crossref","first-page":"524","DOI":"10.3390\/toxins13080524","article-title":"Acute hepatopancreatic necrosis disease (AHPND): virulence, pathogenesis and mitigation strategies in shrimp aquaculture","volume":"13","author":"Kumar","year":"2021","journal-title":"Toxins"},{"key":"10.1016\/j.fsi.2025.110568_bib4","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s12985-025-02637-6","article-title":"Characterization and genome analysis of lytic vibrio phage VPK8 with potential in lysing Vibrio parahaemolyticus isolates from clinical and seafood sources","volume":"22","author":"Jintasakul","year":"2025","journal-title":"Virol. J."},{"key":"10.1016\/j.fsi.2025.110568_bib5","first-page":"48","article-title":"Effect of water temperature on susceptibility of culture marine fish species to vibriosis","volume":"3","author":"Albert","year":"2013","journal-title":"Int. J. Res. Pure Appl. Microbiol."},{"key":"10.1016\/j.fsi.2025.110568_bib6","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.3390\/biology11091268","article-title":"Effect of an oral bivalent vaccine on immune response and immune gene profiling in vaccinated red tilapia (oreochromis spp.) during infections with Streptococcus iniae and Aeromonas hydrophila","volume":"11","author":"Monir","year":"2022","journal-title":"Biology"},{"key":"10.1016\/j.fsi.2025.110568_bib7","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1186\/s12917-025-04548-8","article-title":"Systematic review and meta-analysis of antimicrobial resistant bacteria in free-ranging wild mammals","volume":"21","author":"Smoglica","year":"2025","journal-title":"BMC Vet. Res."},{"key":"10.1016\/j.fsi.2025.110568_bib8","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.imlet.2020.07.002","article-title":"A review: progress in the development of fish vibrio spp. vaccines","volume":"226","author":"Ji","year":"2020","journal-title":"Immunol. Lett."},{"key":"10.1016\/j.fsi.2025.110568_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2022.738560","article-title":"Construction and immunological evaluation of live vector vaccine based on attenuated Listeria monocytogenes vector against Vibrio parahaemolyticus infection","volume":"560","author":"Ji","year":"2022","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110568_bib10","doi-asserted-by":"crossref","first-page":"3644","DOI":"10.1016\/j.vaccine.2013.05.100","article-title":"Outer membrane protein A (OmpA) from Shigella flexneri 2a: a promising subunit vaccine candidate","volume":"31","author":"Pore","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2025.110568_bib11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.molimm.2016.08.011","article-title":"OmpN, outer membrane proteins of Edwardsiella ictaluri are potential vaccine candidates for channel catfish (Ictalurus punctatus)","volume":"78","author":"Yang","year":"2016","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2025.110568_bib12","doi-asserted-by":"crossref","DOI":"10.1038\/srep38577","article-title":"LptD is a promising vaccine antigen and potential immunotherapeutic target for protection against vibrio species infection","volume":"6","author":"Zha","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fsi.2025.110568_bib13","doi-asserted-by":"crossref","first-page":"6115","DOI":"10.1016\/j.vaccine.2014.08.077","article-title":"Identification of a novel vaccine candidate by immunogenic screening of Vibrio parahaemolyticus outer membrane proteins","volume":"32","author":"Li","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2025.110568_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmb.2025.168960","article-title":"Phyre2.2: a community resource for template-based protein structure prediction","author":"Powell","year":"2025","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.fsi.2025.110568_bib15","doi-asserted-by":"crossref","DOI":"10.1088\/1361-6528\/ac659b","article-title":"LptD-antigen system on gold nanoparticles: an innovative strategy in the nanovaccine development","volume":"33","author":"Aguilera-Ju\u00e1rez","year":"2022","journal-title":"Nanotechnology"},{"key":"10.1016\/j.fsi.2025.110568_bib16","doi-asserted-by":"crossref","first-page":"3137","DOI":"10.1128\/iai.63.8.3137-3142.1995","article-title":"A conserved Aeromonas salmonicida porin provides protective immunity to rainbow trout","volume":"63","author":"Lutwyche","year":"1995","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fsi.2025.110568_bib17","doi-asserted-by":"crossref","first-page":"183","DOI":"10.3389\/fcimb.2020.00183","article-title":"A T3SS regulator mutant of Vibrio alginolyticus affects antibiotic susceptibilities and provides significant protection to danio rerio as a live attenuated vaccine","volume":"10","author":"Zhou","year":"2020","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.fsi.2025.110568_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2021.105267","article-title":"Structure, regulation, and host interaction of outer membrane protein U (OmpU) of Vibrio species","volume":"162","author":"Ganie","year":"2022","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.fsi.2025.110568_bib19","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1007\/s11033-012-2252-1","article-title":"Comparison of splenic transcriptome activity of two rainbow trout strains differing in robustness under regional aquaculture conditions","volume":"40","author":"K\u00f6bis","year":"2013","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.fsi.2025.110568_bib20","doi-asserted-by":"crossref","first-page":"48","DOI":"10.3390\/cells6040048","article-title":"Differential location and distribution of hepatic immune cells","volume":"6","author":"Freitas-Lopes","year":"2017","journal-title":"Cells"},{"key":"10.1016\/j.fsi.2025.110568_bib21","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.fsi.2018.03.024","article-title":"Selenium deficiency impaired immune function of the immune organs in young grass carp (Ctenopharyngodon idella)","volume":"77","author":"Zheng","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110568_bib22","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.fsi.2018.05.029","article-title":"Immune response and protective effect against spring viremia of carp virus induced by intramuscular vaccination with a SWCNTs-DNA vaccine encoding matrix protein","volume":"79","author":"Zhang","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110568_bib23","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.fsi.2022.09.053","article-title":"Induction of attenuated Nocardia seriolae and their use as live vaccine trials against fish nocardiosis","volume":"131","author":"Li","year":"2022","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110568_bib24","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.fsi.2004.09.006","article-title":"Innate immunity of fish (overview)","volume":"20","author":"Magnad\u00f3ttir","year":"2006","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110568_bib25","doi-asserted-by":"crossref","DOI":"10.1098\/rstb.2015.0023","article-title":"Outer membrane protein biogenesis in Gram-negative bacteria","volume":"370","author":"Rollauer","year":"2015","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"10.1016\/j.fsi.2025.110568_bib26","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1146\/annurev-micro-090816-093754","article-title":"Outer membrane biogenesis","volume":"71","author":"Konovalova","year":"2017","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.fsi.2025.110568_bib27","series-title":"Biotechnological Advances in Aquaculture Health Management","first-page":"43","article-title":"Biotechnological advances in the development of outer membrane protein-based vaccines for use in aquaculture","author":"Maiti","year":"2021"},{"key":"10.1016\/j.fsi.2025.110568_bib28","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1146\/annurev.micro.61.080706.093245","article-title":"Biogenesis of the gram-negative bacterial outer membrane","volume":"61","author":"Bos","year":"2007","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.fsi.2025.110568_bib29","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.vetmic.2012.08.019","article-title":"The OmpA family of proteins: roles in bacterial pathogenesis and immunity","volume":"163","author":"Confer","year":"2013","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.fsi.2025.110568_bib30","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1006\/fsim.1999.0245","article-title":"Outer membrane proteins of Aeromonas hydrophila induce protective immunity in goldfish","volume":"10","author":"Rahman","year":"2000","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110568_bib31","doi-asserted-by":"crossref","first-page":"495","DOI":"10.3390\/cells10030495","article-title":"Thinking outside the bug: targeting outer membrane proteins for burkholderia vaccines","volume":"10","author":"Grund","year":"2021","journal-title":"Cells"},{"key":"10.1016\/j.fsi.2025.110568_bib32","doi-asserted-by":"crossref","first-page":"8593","DOI":"10.1128\/JB.01163-07","article-title":"Phenotypic characterization of pore mutants of the Vibrio cholerae porin OmpU","volume":"189","author":"Pagel","year":"2007","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.fsi.2025.110568_bib33","doi-asserted-by":"crossref","first-page":"e00809","DOI":"10.1128\/IAI.00809-18","article-title":"Differential recognition of Vibrio parahaemolyticus OmpU by toll-like receptors in monocytes and macrophages for the induction of proinflammatory responses","volume":"87","author":"Gulati","year":"2019","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fsi.2025.110568_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.109409","article-title":"OmpU and OmpC are the key OMPs for litopenaeus vannamei hemocyanin recognizes Vibrio parahaemolyticus","volume":"146","author":"Zhao","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110568_bib35","article-title":"Identification of Vibrio parahaemolyticus and vibrio spp. specific outer membrane proteins by reverse vaccinology and surface proteome","volume":"11","author":"Wang","year":"2020","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.fsi.2025.110568_bib36","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1111\/jfd.12036","article-title":"Cloning, expression of Vibrio alginolyticus outer membrane protein-OmpU gene and its potential application as vaccine in crimson snapper, lutjanus erythropterus bloch","volume":"36","author":"Cai","year":"2013","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2025.110568_bib37","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1111\/j.1462-2920.2009.02138.x","article-title":"The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas","volume":"12","author":"Duperthuy","year":"2010","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.fsi.2025.110568_bib38","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1038\/nature13464","article-title":"Structural basis for outer membrane lipopolysaccharide insertion","volume":"511","author":"Dong","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2025.110568_bib39","doi-asserted-by":"crossref","first-page":"15","DOI":"10.17843\/rpmesp.2022.391.9292","article-title":"Cloning, expression and seroreactivity of the recombinant lipopolysaccharide assembly protein - d (LptD) from Bartonella bacilliformis","volume":"39","author":"Flores-Nu\u00f1ez","year":"2022","journal-title":"Rev. Peru. Med. Exp. Salud Publica"},{"key":"10.1016\/j.fsi.2025.110568_bib40","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/j.fsi.2018.12.030","article-title":"Protection against Vibrio alginolyticus in pearl gentian grouper (\u2640Epinephelus fuscoguttatus \u00d7 \u2642Epinephelus lanceolatu) immunized with an acfA-deletion live attenuated vaccine","volume":"86","author":"Chen","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110568_bib41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.micres.2011.02.002","article-title":"Recombinant outer membrane protein A (OmpA) of Edwardsiella tarda, a potential vaccine candidate for fish, common carp","volume":"167","author":"Maiti","year":"2011","journal-title":"Microbiol. Res."},{"key":"10.1016\/j.fsi.2025.110568_bib42","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.3389\/fimmu.2018.02003","article-title":"Molecular characterization, phylogenetic, expression, and protective immunity analysis of ompf, a promising candidate immunogen against Yersinia ruckeri infection in channel catfish","volume":"9","author":"Wang","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2025.110568_bib43","doi-asserted-by":"crossref","first-page":"3559","DOI":"10.1111\/are.14311","article-title":"Protective immune responses of recombinant outer membrane proteins OmpF and OmpK of Aeromonas hydrophila in European eel (Anguilla Anguilla)","volume":"50","author":"Zhang","year":"2019","journal-title":"Aquac. Res."}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464825004577?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464825004577?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T03:37:42Z","timestamp":1777606662000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464825004577"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":43,"alternative-id":["S1050464825004577"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2025.110568","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of a novel live attenuated vaccine based on epitope-fused OmpU1-LptDEP antigen for vibriosis control in aquaculture","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2025.110568","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"110568"}}