{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T22:23:35Z","timestamp":1757629415620,"version":"3.44.0"},"reference-count":35,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"am","delay-in-days":282,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T00:00:00Z","timestamp":1761955200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100009037","name":"SATREPS","doi-asserted-by":"publisher","award":["JPMJSA1806"],"award-info":[{"award-number":["JPMJSA1806"]}],"id":[{"id":"10.13039\/501100009037","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2025,11]]},"DOI":"10.1016\/j.fsi.2025.110650","type":"journal-article","created":{"date-parts":[[2025,8,9]],"date-time":"2025-08-09T06:38:59Z","timestamp":1754721539000},"page":"110650","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Functional characterization of colony-stimulating factors in cytokine modulation and phagocytic cell activation in Japanese flounder"],"prefix":"10.1016","volume":"166","author":[{"given":"Rungnapa","family":"Sakulworakan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reiko","family":"Nozaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8890-8229","authenticated-orcid":false,"given":"Keiichiro","family":"Koiwai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikuo","family":"Hirono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5102-6831","authenticated-orcid":false,"given":"Hidehiro","family":"Kondo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2025.110650_bib1","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1242\/jeb.038315","article-title":"Understanding the vertebrate immune system: insights from the reptilian perspective","volume":"213","author":"Zimmerman","year":"2010","journal-title":"J. Exp. Biol."},{"key":"10.1016\/j.fsi.2025.110650_bib2","doi-asserted-by":"crossref","DOI":"10.1155\/2017\/9042851","article-title":"Phagocytosis: a fundamental process in immunity","volume":"2017","author":"Rosales","year":"2017","journal-title":"BioMed Res. Int."},{"key":"10.1016\/j.fsi.2025.110650_bib3","first-page":"9723","article-title":"Neutrophils and macrophages as targets for development of nanotherapeutics","volume":"21","author":"Uribe-Querol","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fsi.2025.110650_bib4","first-page":"3897","article-title":"Assessment of phagocytic activity of human peripheral blood without hemolysis","volume":"37","author":"Zhang","year":"2017","journal-title":"Anticancer Res."},{"key":"10.1016\/j.fsi.2025.110650_bib5","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.fsi.2019.03.015","article-title":"Granulocyte colony stimulating factor (GCSF) of Japanese flounder (Paralichthys olivaceus): immunoregulatory property and anti-infectious function","volume":"89","author":"Du","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110650_bib6","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1038\/nri2356","article-title":"Colony-stimulating factors in inflammation and autoimmunity","volume":"8","author":"Hamilton","year":"2008","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.fsi.2025.110650_bib7","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1235370","article-title":"Molecular characterization, gene expression and functional analysis of goldfish (Carassius auratus L.) macrophage colony stimulating factor 2","volume":"14","author":"Gouife","year":"2023","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2025.110650_bib8","doi-asserted-by":"crossref","first-page":"3310","DOI":"10.4049\/jimmunol.181.5.3310","article-title":"Two macrophage colony-stimulating factor genes exist in fish that differ in gene organization and are differentially expressed","volume":"181","author":"Wang","year":"2008","journal-title":"J. Immunol."},{"key":"10.1016\/j.fsi.2025.110650_bib9","series-title":"Molecular Innate Immunity in Teleost Fish: Review and Future Perspectives, Fisheries for Global Welfare and Environment, 5th World Fisheries Congress","first-page":"263","author":"Aoki","year":"2008"},{"key":"10.1016\/j.fsi.2025.110650_bib10","doi-asserted-by":"crossref","first-page":"174","DOI":"10.3389\/fcell.2018.00174","article-title":"Hematopoietic cytokine gene duplication in zebrafish erythroid and myeloid lineages","volume":"6","author":"Oltova","year":"2018","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.fsi.2025.110650_bib11","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.3389\/fimmu.2018.01105","article-title":"Mechanisms of fish macrophage antimicrobial immunity","volume":"9","author":"Grayfer","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2025.110650_bib12","first-page":"115","article-title":"Immune relevant genes of Japanese flounder, Paralichthys olivaceus","volume":"1","author":"Aoki","year":"2006","journal-title":"Comp. Biochem. Physiol. D: Genom. Proteonomics"},{"key":"10.1016\/j.fsi.2025.110650_bib13","article-title":"A novel microRNA of Japanese flounder regulates antimicrobial immunity involving a bacteria-binding CSF3","volume":"12","author":"Li","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2025.110650_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.109803","article-title":"Application of nervous necrosis virus capsid protein-based antigen-presenting particles for vaccine development","volume":"152","author":"Wayha","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110650_bib15","doi-asserted-by":"crossref","unstructured":"M.R. Green, J. Sambrook, Preparation of plasmid DNA by alkaline lysis with sodium dodecyl sulfate: maxipreps, Cold Spring Harb. Protoc. 2018 (2018) pdb.prot093351. https:\/\/doi.org\/10.1101\/pdb.prot093351.","DOI":"10.1101\/pdb.prot093351"},{"key":"10.1016\/j.fsi.2025.110650_bib16","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1182\/bloodadvances.2021005459","article-title":"M-CSFR\/CSF1R signaling regulates myeloid fates in zebrafish via distinct action of its receptors and ligands","volume":"6","author":"Hason","year":"2021","journal-title":"Blood Adv."},{"key":"10.1016\/j.fsi.2025.110650_bib17","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1093\/molbev\/msk003","article-title":"Asymmetric evolution in two fish-specifically duplicated receptor tyrosine kinase paralogons involved in teleost coloration","volume":"23","author":"Braasch","year":"2006","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.fsi.2025.110650_bib18","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.fsi.2004.09.005","article-title":"Ontogeny of the immune system of fish","volume":"20","author":"Zapata","year":"2006","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110650_bib19","doi-asserted-by":"crossref","first-page":"255","DOI":"10.3389\/fimmu.2019.00255","article-title":"Paralogs of common carp granulocyte colony-stimulating factor (G-CSF) have different functions regarding development, trafficking and activation of neutrophils","volume":"10","author":"Katakura","year":"2019","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2025.110650_bib20","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1093\/molbev\/msy230","article-title":"Insights into the evolution of the suppressors of cytokine signaling (SOCS) gene family in vertebrates","volume":"36","author":"Wang","year":"2019","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.fsi.2025.110650_bib21","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.4049\/jimmunol.1502690","article-title":"Changes in gene expression during G-CSF\u2013induced emergency granulopoiesis in humans","volume":"197","author":"Pedersen","year":"2016","journal-title":"J. Immunol."},{"key":"10.1016\/j.fsi.2025.110650_bib22","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1084\/jem.184.1.61","article-title":"The transcription factor PU.1 is involved in macrophage proliferation","volume":"184","author":"Celada","year":"1996","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.fsi.2025.110650_bib23","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.3390\/cancers11071037","article-title":"AP-1 transcription factors as regulators of immune responses in cancer","volume":"11","author":"Atsaves","year":"2019","journal-title":"Cancers"},{"key":"10.1016\/j.fsi.2025.110650_bib24","first-page":"373","article-title":"The macrophage transcription factor PU.1 directs tissue-specific expression of the macrophage colony-stimulating factor receptor","volume":"14","author":"Zhang","year":"1994","journal-title":"Mol. Cell Biol."},{"key":"10.1016\/j.fsi.2025.110650_bib25","doi-asserted-by":"crossref","first-page":"4456","DOI":"10.1093\/emboj\/17.15.4456","article-title":"PU.1 regulates both cytokine-dependent proliferation and differentiation of granulocyte\/macrophage progenitors","volume":"17","author":"DeKoter","year":"1998","journal-title":"EMBO J."},{"key":"10.1016\/j.fsi.2025.110650_bib26","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.aax0217","article-title":"Durable multitransgene expression in vivo using systemic, nonviral DNA delivery","volume":"5","author":"Handumrongkul","year":"2019","journal-title":"Sci. Adv."},{"key":"10.1016\/j.fsi.2025.110650_bib27","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.cyto.2015.05.030","article-title":"GM-CSF: an immune modulatory cytokine that can suppress autoimmunity","volume":"75","author":"Bhattacharya","year":"2015","journal-title":"Cytokine"},{"key":"10.1016\/j.fsi.2025.110650_bib28","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1182\/blood.V71.6.1529.1529","article-title":"Independent regulation of M-CSF and G-CSF gene expression in human monocytes","volume":"71","author":"Vellenga","year":"1988","journal-title":"Blood"},{"key":"10.1016\/j.fsi.2025.110650_bib29","doi-asserted-by":"crossref","first-page":"11254","DOI":"10.1128\/JVI.74.23.11254-11261.2000","article-title":"Characterization of promoter function and cell-type-specific expression from viral vectors in the nervous system","volume":"74","author":"Smith","year":"2000","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110650_bib30","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1182\/blood-2017-06-780882","article-title":"Inflammation: a key regulator of hematopoietic stem cell fate in health and disease","volume":"130","author":"Pietras","year":"2017","journal-title":"Blood"},{"year":"2015","series-title":"In Vitro Effects of Different Innate-Immune Stimulants on Chinook Salmon Embryo Cell Line (CHSE-214): Transcriptional and Translational Studies","author":"Est\u00e9vez","key":"10.1016\/j.fsi.2025.110650_bib31"},{"key":"10.1016\/j.fsi.2025.110650_bib32","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.dci.2018.07.010","article-title":"Characterisation of rainbow trout peripheral blood leucocytes prepared by hypotonic lysis of erythrocytes, and analysis of their phagocytic activity, proliferation and response to PAMPs and proinflammatory cytokines","volume":"88","author":"Hu","year":"2018","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2025.110650_bib33","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.636585","article-title":"Damage-induced calcium signaling and reactive oxygen species mediate macrophage activation in zebrafish","volume":"12","author":"Sipka","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2025.110650_bib34","doi-asserted-by":"crossref","first-page":"2529","DOI":"10.1007\/s10616-016-9974-1","article-title":"Shear stress increases cytotoxicity and reduces transfection efficiency of liposomal gene delivery to CHO-S cells","volume":"68","author":"Rawat","year":"2016","journal-title":"Cytotechnology"},{"key":"10.1016\/j.fsi.2025.110650_bib35","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2020.103920","article-title":"Macrophage colony stimulating factor (MCSF) of Japanese flounder (Paralichthys olivaceus): immunoregulatory property, anti-infectious function, and interaction with MCSF receptor","volume":"116","author":"Gu","year":"2021","journal-title":"Dev. Comp. Immunol."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S105046482500539X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S105046482500539X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T14:38:26Z","timestamp":1757515106000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S105046482500539X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11]]},"references-count":35,"alternative-id":["S105046482500539X"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2025.110650","relation":{},"ISSN":["1050-4648"],"issn-type":[{"type":"print","value":"1050-4648"}],"subject":[],"published":{"date-parts":[[2025,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Functional characterization of colony-stimulating factors in cytokine modulation and phagocytic cell activation in Japanese flounder","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2025.110650","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"110650"}}