{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T17:10:32Z","timestamp":1792429832704,"version":"4.5.0"},"reference-count":222,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014166","name":"Philippine Council for Agriculture Aquatic and Natural Resources Research and Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.fsi.2025.110877","type":"journal-article","created":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T13:52:48Z","timestamp":1757771568000},"page":"110877","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["A comparative review of RNAi delivery systems for shrimp aquaculture and future directions"],"prefix":"10.1016","volume":"167","author":[{"given":"Ivane R.","family":"Pedrosa-Gerasmio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krisha Marie D.L.","family":"Saquilayan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4316-8575","authenticated-orcid":false,"given":"Patricia Jhoanna V.","family":"Glori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debrah Jannsen DJ N.","family":"Almazan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim Troy A.","family":"Solitario","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2599-6417","authenticated-orcid":false,"given":"Mary Beth B.","family":"Maningas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2025.110877_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2024.741128","article-title":"Identification of active shrimp U6 promoters for efficient CRISPR\/Cas9 gene editing in penaeid shrimps based on a vp28-pseudotyped baculovirus expression system","author":"Song","year":"2024","journal-title":"Aquaculture"},{"issue":"7","key":"10.1016\/j.fsi.2025.110877_bib2","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0307976","article-title":"Structural modelling and preventive strategy targeting of WSSV hub proteins to combat viral infection in shrimp Penaeus monodon","volume":"19","author":"Panrat","year":"2024","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2025.110877_bib3","doi-asserted-by":"crossref","first-page":"2061","DOI":"10.1007\/s00705-019-04265-2","article-title":"Global distribution of white spot syndrome virus genotypes determined using a novel genotyping assay","volume":"164","author":"Oakey","year":"2019","journal-title":"Arch. Virol."},{"issue":"10","key":"10.1016\/j.fsi.2025.110877_bib4","doi-asserted-by":"crossref","first-page":"2923","DOI":"10.1007\/s00705-017-3450-x","article-title":"Recent progress in the development of white spot syndrome virus vaccines for protecting shrimp against viral infection","volume":"162","author":"Feng","year":"2017","journal-title":"Arch. Virol."},{"issue":"10","key":"10.1016\/j.fsi.2025.110877_bib5","doi-asserted-by":"crossref","first-page":"2050","DOI":"10.3390\/v15102050","article-title":"RNAi-based therapy: combating shrimp viral diseases","volume":"15","author":"Alam","year":"2023","journal-title":"Viruses"},{"key":"10.1016\/j.fsi.2025.110877_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2024.741570","article-title":"Delivery of virus-specific dsRNA using a composite nanomaterial improves the protection of shrimp (Litopenaeus vannamei) against yellow head virus challenge","volume":"595","author":"Suksai","year":"2025","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110877_bib7","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.jip.2016.11.006","article-title":"Potential of RNAi applications to control viral diseases of farmed shrimp","volume":"147","author":"Itsathitphaisarn","year":"2017","journal-title":"J. Invertebr. Pathol."},{"issue":"11","key":"10.1016\/j.fsi.2025.110877_bib8","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.3390\/biom14111358","article-title":"RNA interference applied to crustacean aquaculture","volume":"14","author":"Fajardo","year":"2024","journal-title":"Biomolecules"},{"issue":"6669","key":"10.1016\/j.fsi.2025.110877_bib9","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1038\/35888","article-title":"Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans","volume":"391","author":"Fire","year":"1998","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2025.110877_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbpa.2024.111703","article-title":"Knock down to level up: reframing RNAi for invertebrate ecophysiology","author":"Lebenson","year":"2024","journal-title":"Comp. Biochem. Physiol. Mol. Integr. Physiol."},{"issue":"4","key":"10.1016\/j.fsi.2025.110877_bib11","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S1046-2023(03)00050-1","article-title":"Genome-wide RNAi screening in Caenorhabditis elegans","volume":"30","author":"Kamath","year":"2003","journal-title":"Methods"},{"issue":"5","key":"10.1016\/j.fsi.2025.110877_bib12","doi-asserted-by":"crossref","first-page":"613","DOI":"10.2144\/000112792","article-title":"RNAi mechanisms and applications","volume":"44","author":"Kim","year":"2008","journal-title":"Biotechniques"},{"issue":"15","key":"10.1016\/j.fsi.2025.110877_bib13","doi-asserted-by":"crossref","first-page":"4343","DOI":"10.1021\/acs.jafc.1c00268","article-title":"RNA interference in aquaculture: a small tool for big potential","volume":"69","author":"Abo-Al-Ela","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.fsi.2025.110877_bib14","article-title":"The quest for the best target genes for RNAi\u2010mediated pest control","author":"Cedden","year":"2024","journal-title":"Insect Mol. Biol."},{"key":"10.1016\/j.fsi.2025.110877_bib15","article-title":"RNAi-mediated knockdown of the aquaporin 4 gene impairs salinity tolerance and delays the molting process in Pacific white shrimp, Litopenaeus vannamei","volume":"35","author":"Zhao","year":"2024","journal-title":"Aquac. Rep."},{"issue":"7","key":"10.1016\/j.fsi.2025.110877_bib16","doi-asserted-by":"crossref","first-page":"1074","DOI":"10.3390\/ani14071074","article-title":"Identification of growth-related gene BAMBI and analysis of gene structure and function in the Pacific white shrimp Litopenaeus vannamei","volume":"14","author":"Niu","year":"2024","journal-title":"Animals"},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib17","doi-asserted-by":"crossref","first-page":"3164","DOI":"10.1038\/s41598-019-39539-x","article-title":"Generation of microalga Chlamydomonas reinhardtii expressing shrimp antiviral dsRNA without supplementation of antibiotics","volume":"9","author":"Charoonnart","year":"2019","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib18","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.bbrc.2005.06.063","article-title":"Silencing of yellow head virus replication in penaeid shrimp cells by dsRNA","volume":"334","author":"Tirasophon","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"2","key":"10.1016\/j.fsi.2025.110877_bib19","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1007\/s10126-007-9052-y","article-title":"Silencing vp28 gene of white spot syndrome virus of shrimp by bacterially expressed dsRNA","volume":"10","author":"Sarathi","year":"2008","journal-title":"Mar. Biotechnol."},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib20","doi-asserted-by":"crossref","DOI":"10.1088\/1755-1315\/860\/1\/012031","article-title":"Effect of different doses of dsRNA vp15 vaccine for controlling white spot syndrome virus infection in tiger shrimp Penaeus monodon","volume":"860","author":"Parenrengi","year":"2021","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"issue":"2","key":"10.1016\/j.fsi.2025.110877_bib21","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1016\/j.jksus.2014.11.004","article-title":"Efficacy of double-stranded RNA against white spot syndrome virus (WSSV) non-structural (orf89, wsv191) and structural (vp28, vp26) genes in the Pacific white shrimp Litopenaeus vannamei","volume":"27","author":"Escobedo-Bonilla","year":"2015","journal-title":"J. King Saud Univ. Sci."},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib22","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.fsi.2014.06.008","article-title":"RNA interference technology used for the study of aquatic virus infections","volume":"40","author":"Reshi","year":"2014","journal-title":"Fish Shellfish Immunol."},{"issue":"4","key":"10.1016\/j.fsi.2025.110877_bib23","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1007\/s10126-007-9073-6","article-title":"Suppression of PmRab7 by dsRNA inhibits WSSV or YHV infection in shrimp","volume":"10","author":"Ongvarrasopone","year":"2008","journal-title":"Mar. Biotechnol."},{"key":"10.1016\/j.fsi.2025.110877_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2024.740763","article-title":"Non-invasive dsRNA delivery via feeding for effective gene silencing in teleost fish: a novel approach in the study of gene function analysis","volume":"586","author":"Gao","year":"2024","journal-title":"Aquaculture"},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib25","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.mrrev.2004.06.002","article-title":"Know-how of RNA Interference and its applications in research and therapy","volume":"567","author":"Wadhwa","year":"2004","journal-title":"Mutat. Res. Rev. Mutat. Res."},{"issue":"2","key":"10.1016\/j.fsi.2025.110877_bib26","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1038\/35052556","article-title":"Post-transcriptional gene silencing by double-stranded RNA","volume":"2","author":"Hammond","year":"2001","journal-title":"Nat. Rev. Genet."},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib27","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jip.2011.02.002","article-title":"Double-stranded RNA against White Spot Syndrome Virus (WSSV) vp28 or vp26 reduced susceptibility of Litopenaeus vannamei to WSSV, and survivors exhibited decreased susceptibility in subsequent re-infections","volume":"107","author":"Mejia-Ruiz","year":"2011","journal-title":"J. Invertebr. Pathol."},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib28","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1186\/s12896-015-0226-9","article-title":"Large-scale production and antiviral efficacy of multi-target double-stranded RNA for the prevention of white spot syndrome virus (WSSV) in shrimp","volume":"15","author":"Thammasorn","year":"2015","journal-title":"BMC Biotechnol."},{"key":"10.1016\/j.fsi.2025.110877_bib29","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.jbiotec.2020.06.022","article-title":"A multi-target dsRNA for simultaneous inhibition of yellow head virus and white spot syndrome virus in shrimp","volume":"321","author":"Chaimongkon","year":"2020","journal-title":"J. Biotechnol."},{"issue":"7","key":"10.1016\/j.fsi.2025.110877_bib30","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1128\/AEM.07486-11","article-title":"Caenorhabditis elegans, a model organism for investigating immunity","volume":"78","author":"Marsh","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"issue":"5","key":"10.1016\/j.fsi.2025.110877_bib31","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1016\/0092-8674(93)90529-Y","article-title":"The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14","volume":"75","author":"Lee","year":"1993","journal-title":"Cell"},{"issue":"5","key":"10.1016\/j.fsi.2025.110877_bib32","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/0092-8674(93)90530-4","article-title":"Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans","volume":"75","author":"Wightman","year":"1993","journal-title":"Cell"},{"issue":"2","key":"10.1016\/j.fsi.2025.110877_bib33","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1093\/genetics\/96.2.435","article-title":"Isolation and genetic characterization of cell-lineage mutants of the nematode Caenorhabditis elegans","volume":"96","author":"Horvitz","year":"1980","journal-title":"Genetics"},{"issue":"4","key":"10.1016\/j.fsi.2025.110877_bib34","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1101\/gad.1.4.398","article-title":"The lin-14 locus of Caenorhabditis elegans controls the time of expression of specific postembryonic developmental events","volume":"1","author":"Ambros","year":"1987","journal-title":"Gene Dev."},{"key":"10.1016\/j.fsi.2025.110877_bib35","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/326259a0","article-title":"A genetic pathway for the specification of the vulval cell lineages of Caenorhabditis elegans","volume":"326","author":"Ferguson","year":"1987","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2025.110877_bib36","doi-asserted-by":"crossref","first-page":"S89","DOI":"10.1016\/S0092-8674(04)00035-2","article-title":"A short history of a short RNA","volume":"116","author":"Lee","year":"2004","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2025.110877_bib37","first-page":"1","article-title":"MicroRNA history: discovery, recent applications, and next frontiers","volume":"717","author":"Almeida","year":"2011","journal-title":"Mutat. Res."},{"key":"10.1016\/j.fsi.2025.110877_bib38","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/0092-8674(93)90530-4","article-title":"Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans","volume":"75","author":"Wightman","year":"1993","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2025.110877_bib39","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1038\/35040556","article-title":"Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA","volume":"408","author":"Pasquinelli","year":"2000","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2025.110877_bib40","first-page":"381","article-title":"Mechanisms of control of microRNA biogenesis","volume":"148","author":"Davis-Dusenbery","year":"2010","journal-title":"J. Biochem."},{"key":"10.1016\/j.fsi.2025.110877_bib41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ygeno.2010.03.009","article-title":"Identification of homologous microRNAs in 56 animal genomes","volume":"96","author":"Li","year":"2010","journal-title":"Genomics"},{"key":"10.1016\/j.fsi.2025.110877_bib42","doi-asserted-by":"crossref","first-page":"R57","DOI":"10.1186\/gb-2014-15-4-r57","article-title":"Evidence for the biogenesis of more than 1,000 novel human microRNAs","volume":"15","author":"Friedl\u00e4nder","year":"2014","journal-title":"Genome Biol."},{"key":"10.1016\/j.fsi.2025.110877_bib43","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1002\/jcp.25056","article-title":"MicroRNA as biomarkers and diagnostics","volume":"231","author":"Wang","year":"2016","journal-title":"J. Cell. Physiol."},{"key":"10.1016\/j.fsi.2025.110877_bib44","first-page":"57","article-title":"Modulation of microRNA processing by Dicer via its associated dsRNA binding proteins","volume":"7","author":"Yoshida","year":"2021","journal-title":"Noncoding RNA"},{"key":"10.1016\/j.fsi.2025.110877_bib45","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.ceb.2009.04.009","article-title":"Mechanisms of miRNA-mediated post-transcriptional regulation in animal cells","volume":"21","author":"Chekulaeva","year":"2009","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.fsi.2025.110877_bib46","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1002\/1873-3468.13067","article-title":"Regulation of primary microRNA processing","volume":"592","author":"Creugny","year":"2018","journal-title":"FEBS Lett."},{"key":"10.1016\/j.fsi.2025.110877_bib47","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/35005107","article-title":"An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells","volume":"404","author":"Hammond","year":"2000","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2025.110877_bib48","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2020.103907","article-title":"RNAi-based antiviral immunity of shrimp","volume":"115","author":"Gong","year":"2021","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2025.110877_bib49","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1016\/j.cell.2009.01.035","article-title":"Origins and mechanisms of miRNA and siRNA","volume":"136","author":"Carthew","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2025.110877_bib50","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1042\/BCJ20160759","article-title":"Molecular mechanisms of Dicer: endonuclease and enzymatic activity","volume":"474","author":"Song","year":"2017","journal-title":"Biochem. J."},{"key":"10.1016\/j.fsi.2025.110877_bib51","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","article-title":"MicroRNAs: target recognition and regulatory functions","volume":"136","author":"Bartel","year":"2009","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2025.110877_bib52","doi-asserted-by":"crossref","first-page":"12997","DOI":"10.1128\/JVI.01702-12","article-title":"Functional analysis of a crustacean microRNA in host\u2013virus interactions","volume":"86","author":"Huang","year":"2012","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110877_bib53","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1186\/1471-2164-13-159","article-title":"Characterization of host microRNAs that respond to DNA virus infection in a crustacean","volume":"13","author":"Huang","year":"2012","journal-title":"BMC Genom."},{"key":"10.1016\/j.fsi.2025.110877_bib54","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.molimm.2015.07.008","article-title":"Roles of small RNAs in the immune defense mechanisms of crustaceans","volume":"68","author":"He","year":"2015","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2025.110877_bib55","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1111\/raq.12187","article-title":"RNA interference in shrimp and potential applications in aquaculture","volume":"10","author":"Nguyen","year":"2016","journal-title":"Rev. Aquacult."},{"key":"10.1016\/j.fsi.2025.110877_bib56","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.cell.2007.12.024","article-title":"Getting to the root of miRNA-mediated gene silencing","volume":"132","author":"Eulalio","year":"2008","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2025.110877_bib57","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/j.molcel.2004.05.027","article-title":"Computational identification of plant microRNAs and their targets, including a stress-induced miRNA","volume":"14","author":"Jones-Rhoades","year":"2004","journal-title":"Mol. Cell"},{"key":"10.1016\/j.fsi.2025.110877_bib58","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.4161\/rna.20741","article-title":"Comprehensive characterization of viral miRNAs involved in white spot syndrome virus (WSSV) infection","volume":"9","author":"He","year":"2012","journal-title":"RNA Biol."},{"key":"10.1016\/j.fsi.2025.110877_bib59","doi-asserted-by":"crossref","first-page":"1104","DOI":"10.1128\/JVI.02455-13","article-title":"Viral microRNAs targeting virus genes promote virus infection in shrimp in vivo","volume":"88","author":"He","year":"2014","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110877_bib60","doi-asserted-by":"crossref","first-page":"2854","DOI":"10.1096\/fj.201601141RR","article-title":"Noncoding miRNAs bridge virus infection and host autophagy in shrimp in vivo","volume":"31","author":"He","year":"2017","journal-title":"FASEB J."},{"key":"10.1016\/j.fsi.2025.110877_bib61","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.02261-16","article-title":"Two white spot syndrome virus microRNAs target the dorsal gene to promote virus infection in Marsupenaeus japonicus shrimp","volume":"91","author":"Ren","year":"2017","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110877_bib62","doi-asserted-by":"crossref","first-page":"2999","DOI":"10.3389\/fimmu.2018.02999","article-title":"Shrimp miR-1000 functions in antiviral immunity by simultaneously triggering the degradation of two viral mRNAs","volume":"9","author":"Gong","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2025.110877_bib63","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.tibs.2004.12.007","article-title":"siRNA and miRNA: an insight into RISCs","volume":"30","author":"Tang","year":"2005","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.fsi.2025.110877_bib64","doi-asserted-by":"crossref","first-page":"D154","DOI":"10.1093\/nar\/gkm952","article-title":"miRBase: tools for microRNA genomics","volume":"36","author":"Griffiths-Jones","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.fsi.2025.110877_bib65","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.cell.2006.02.015","article-title":"Pathogen recognition and innate immunity","volume":"124","author":"Akira","year":"2006","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2025.110877_bib66","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.dci.2012.03.016","article-title":"Recent advances in researches on the innate immunity of shrimp in China","volume":"39","author":"Li","year":"2013","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2025.110877_bib67","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1128\/JVI.01794-09","article-title":"Functional analysis of a cellular microRNA in insect host\u2013ascovirus interaction","volume":"84","author":"Hussain","year":"2010","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110877_bib68","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1146\/annurev.micro.112408.134243","article-title":"Viruses, microRNAs, and host interactions","volume":"64","author":"Skalsky","year":"2010","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.fsi.2025.110877_bib69","doi-asserted-by":"crossref","first-page":"2544","DOI":"10.1128\/JVI.03575-13","article-title":"Involvement of viral microRNA in the regulation of antiviral apoptosis in shrimp","volume":"88","author":"Huang","year":"2014","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110877_bib70","doi-asserted-by":"crossref","first-page":"855","DOI":"10.3389\/fimmu.2017.00855","article-title":"Shrimp miR-12 suppresses white spot syndrome virus infection by synchronously triggering antiviral phagocytosis and apoptosis pathways","volume":"8","author":"Shu","year":"2017","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2025.110877_bib71","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1186\/s12929-023-00981-9","article-title":"Small interfering RNA (siRNA)-based therapeutic applications against viruses: principles, potential, and challenges","volume":"30","author":"Kang","year":"2023","journal-title":"J. Biomed. Sci."},{"key":"10.1016\/j.fsi.2025.110877_bib72","series-title":"Molecular Biology of the Cell","author":"Alberts","year":"2000"},{"key":"10.1016\/j.fsi.2025.110877_bib73","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10126-008-9155-0","article-title":"Application of nucleic-acid-based therapeutics for viral infections in shrimp aquaculture","volume":"11","author":"Shekhar","year":"2009","journal-title":"Mar. Biotechnol."},{"key":"10.1016\/j.fsi.2025.110877_bib74","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/s41580-020-00315-9","article-title":"Gene regulation by long non-coding RNAs and its biological functions","volume":"22","author":"Statello","year":"2021","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.fsi.2025.110877_bib75","doi-asserted-by":"crossref","first-page":"884","DOI":"10.3390\/v12080884","article-title":"Silencing and transcriptional regulation of endogenous retroviruses: an overview","volume":"12","author":"Geis","year":"2020","journal-title":"Viruses"},{"key":"10.1016\/j.fsi.2025.110877_bib76","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.fsi.2007.11.006","article-title":"A key gene of the RNA interference pathway in the black tiger shrimp, Penaeus monodon: identification and functional characterisation of Dicer-1","volume":"24","author":"Su","year":"2008","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110877_bib77","series-title":"Biotechnological Advances in Aquaculture Health Management","first-page":"25","article-title":"RNA interference and its potential applications in aquatic animal health management","author":"Kumar","year":"2021"},{"key":"10.1016\/j.fsi.2025.110877_bib78","series-title":"Fish Immune System and Vaccines","first-page":"167","article-title":"RNA interference vaccines for disease control in aquaculture","author":"Gireesh-Babu","year":"2022"},{"key":"10.1016\/j.fsi.2025.110877_bib79","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.dci.2015.03.013","article-title":"Analysis on the expression and function of syndecan in the Pacific white shrimp Litopenaeus vannamei","volume":"51","author":"Yang","year":"2015","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2025.110877_bib80","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.aquaculture.2011.04.003","article-title":"The antiviral vp28-siRNA expressed in bacteria protects shrimp against white spot syndrome virus (WSSV)","volume":"319","author":"Zhu","year":"2011","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110877_bib81","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/s10126-011-9387-2","article-title":"Protection of shrimp against white spot syndrome virus (WSSV) with \u03b2-1,3-D-glucan-encapsulated vp28-siRNA particles","volume":"14","author":"Zhu","year":"2012","journal-title":"Mar. Biotechnol."},{"key":"10.1016\/j.fsi.2025.110877_bib82","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.aquaculture.2007.06.029","article-title":"Inhibition of white spot syndrome virus in Litopenaeus vannamei shrimp by sequence-specific siRNA","volume":"271","author":"Wu","year":"2007","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110877_bib83","doi-asserted-by":"crossref","first-page":"5312","DOI":"10.3390\/ijms23105312","article-title":"RNA interference: promising approach to combat plant viruses","volume":"23","author":"Akbar","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fsi.2025.110877_bib84","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.ymeth.2016.04.003","article-title":"Guidelines for the optimal design of miRNA-based shRNAs","volume":"103","author":"Bofill-De Ros","year":"2016","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2025.110877_bib85","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1038\/ng1650","article-title":"Second-generation shRNA libraries covering the mouse and human genomes","volume":"37","author":"Silva","year":"2005","journal-title":"Nat. Genet."},{"key":"10.1016\/j.fsi.2025.110877_bib86","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1038\/nature01957","article-title":"The nuclear RNase III Drosha initiates microRNA processing","volume":"425","author":"Lee","year":"2003","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2025.110877_bib87","doi-asserted-by":"crossref","first-page":"5875","DOI":"10.1093\/emboj\/cdf582","article-title":"Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP","volume":"21","author":"Zhang","year":"2002","journal-title":"EMBO J."},{"key":"10.1016\/j.fsi.2025.110877_bib88","doi-asserted-by":"crossref","first-page":"4663","DOI":"10.1093\/emboj\/cdf476","article-title":"MicroRNA maturation: stepwise processing and subcellular localization","volume":"21","author":"Lee","year":"2002","journal-title":"EMBO J."},{"key":"10.1016\/j.fsi.2025.110877_bib89","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/j.molcel.2004.12.002","article-title":"Transcription and processing of human microRNA precursors","volume":"16","author":"Cullen","year":"2004","journal-title":"Mol. Cell"},{"key":"10.1016\/j.fsi.2025.110877_bib90","doi-asserted-by":"crossref","first-page":"3011","DOI":"10.1101\/gad.1158803","article-title":"Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs","volume":"17","author":"Yi","year":"2003","journal-title":"Genes Dev."},{"key":"10.1016\/j.fsi.2025.110877_bib91","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1126\/science.1090599","article-title":"Nuclear export of microRNA precursors","volume":"303","author":"Lund","year":"2004","journal-title":"Science"},{"key":"10.1016\/j.fsi.2025.110877_bib92","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0092-8674(04)00261-2","article-title":"Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA\/miRNA silencing pathways","volume":"117","author":"Lee","year":"2004","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2025.110877_bib93","doi-asserted-by":"crossref","first-page":"2580","DOI":"10.1261\/rna.1351608","article-title":"Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs","volume":"14","author":"Landthaler","year":"2008","journal-title":"RNA"},{"key":"10.1016\/j.fsi.2025.110877_bib94","doi-asserted-by":"crossref","first-page":"3849","DOI":"10.1016\/j.vaccine.2009.04.011","article-title":"DNA constructs expressing long-hairpin RNA (lhRNA) protect Penaeus monodon against White Spot Syndrome Virus","volume":"27","author":"Krishnan","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2025.110877_bib95","doi-asserted-by":"crossref","first-page":"263","DOI":"10.3354\/dao02126","article-title":"RNA interference-based therapeutics for shrimp viral diseases","volume":"86","author":"Krishnan","year":"2009","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.fsi.2025.110877_bib96","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1038\/sj.mt.6300077","article-title":"Specific inhibition of HBV replication in vitro and in vivo with expressed long hairpin RNA","volume":"15","author":"Weinberg","year":"2007","journal-title":"Mol. Ther."},{"key":"10.1016\/j.fsi.2025.110877_bib97","doi-asserted-by":"crossref","first-page":"13561","DOI":"10.1128\/JVI.79.21.13561-13571.2005","article-title":"Double-stranded RNA induces sequence-specific antiviral silencing in addition to nonspecific immunity in a marine shrimp: convergence of RNA interference and innate immunity in the invertebrate antiviral response?","volume":"79","author":"Robalino","year":"2005","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110877_bib98","doi-asserted-by":"crossref","first-page":"10442","DOI":"10.1128\/JVI.78.19.10442-10448.2004","article-title":"Induction of antiviral immunity by double-stranded RNA in a marine invertebrate","volume":"78","author":"Robalino","year":"2004","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110877_bib99","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1038\/nbt1369","article-title":"RNA interference against viruses: strike and counterstrike","volume":"25","author":"Haasnoot","year":"2007","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.fsi.2025.110877_bib100","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.jviromet.2009.08.010","article-title":"A novel and inexpensive application of RNAi technology to protect shrimp from viral disease","volume":"162","author":"Saksmerprome","year":"2009","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.fsi.2025.110877_bib101","doi-asserted-by":"crossref","DOI":"10.1016\/j.jip.2020.107442","article-title":"Effective suppression of yellow head virus replication in Penaeus monodon hemocytes using constitutive expression vector for long-hairpin RNA (lhRNA)","volume":"175","author":"Thedcharoen","year":"2020","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.fsi.2025.110877_bib102","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.virusres.2016.01.013","article-title":"Gene silencing of vp9 gene impairs WSSV infectivity on Macrobrachium rosenbergii","volume":"214","author":"Alenton","year":"2016","journal-title":"Virus Res."},{"key":"10.1016\/j.fsi.2025.110877_bib103","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1111\/jwas.12825","article-title":"Zebrafish U6 promoter driving short-hairpin RNA expression for PmRab7 knockdown to inhibit yellow head virus infection in shrimp hemocytes","volume":"53","author":"Thaiprasansup","year":"2022","journal-title":"J. World Aquacult. Soc."},{"key":"10.1016\/j.fsi.2025.110877_bib104","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.fsi.2025.110877_bib105","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.jip.2012.03.002","article-title":"Nucleic-acid based antivirals: augmenting RNA interference to \u2018vaccinate\u2019 Litopenaeus vannamei","volume":"110","author":"Bartholomay","year":"2012","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.fsi.2025.110877_bib106","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1007\/s00705-010-0649-5","article-title":"Inhibition of Penaeus monodon densovirus replication in shrimp by double-stranded RNA","volume":"155","author":"Attasart","year":"2010","journal-title":"Arch. Virol."},{"key":"10.1016\/j.fsi.2025.110877_bib107","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.fsi.2006.10.012","article-title":"Protection of shrimp (Penaeus chinensis) against white spot syndrome virus (WSSV) challenge by double-stranded RNA","volume":"23","author":"Kim","year":"2007","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110877_bib108","doi-asserted-by":"crossref","first-page":"1766","DOI":"10.1080\/15476286.2017.1356567","article-title":"Short versus long double-stranded RNA activation of a post-transcriptional gene knockdown pathway","volume":"14","author":"Shpak","year":"2017","journal-title":"RNA Biol."},{"key":"10.1016\/j.fsi.2025.110877_bib109","doi-asserted-by":"crossref","first-page":"57","DOI":"10.3354\/dao02600","article-title":"Sequence-optimized and targeted double-stranded RNA as a therapeutic antiviral treatment against infectious myonecrosis virus in Litopenaeus vannamei","volume":"105","author":"Loy","year":"2013","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.fsi.2025.110877_bib110","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1016\/j.dci.2010.06.017","article-title":"Non-specific activation of antiviral immunity and induction of RNA interference may engage the same pathway in the Pacific white leg shrimp Litopenaeus vannamei","volume":"34","author":"Labreuche","year":"2010","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2025.110877_bib111","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.antiviral.2006.08.007","article-title":"Silencing shrimp white spot syndrome virus (WSSV) genes by siRNA","volume":"73","author":"Xu","year":"2007","journal-title":"Antivir. Res."},{"key":"10.1016\/j.fsi.2025.110877_bib112","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.virusres.2005.06.006","article-title":"siRNA injection induces sequence-independent protection in Penaeus monodon against white spot syndrome virus","volume":"114","author":"Westenberg","year":"2005","journal-title":"Virus Res."},{"key":"10.1016\/j.fsi.2025.110877_bib113","doi-asserted-by":"crossref","DOI":"10.1016\/j.jip.2022.107869","article-title":"Effects of length and sequence of long double-stranded RNAs targeting ribonucleotide reductase 2 of white spot syndrome virus (WSSV) on protective efficacy against WSSV","volume":"196","author":"Joo Hong","year":"2023","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.fsi.2025.110877_bib114","series-title":"Nanotechnology in Modern Animal Biotechnology","first-page":"67","article-title":"Nanomaterials-based siRNA delivery: routes of administration, hurdles and role of nanocarriers","author":"Gupta","year":"2019"},{"key":"10.1016\/j.fsi.2025.110877_bib115","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000764","article-title":"Six RNA viruses and forty-one hosts: viral small RNAs and modulation of small RNA repertoires in vertebrate and invertebrate systems","volume":"6","author":"Parameswaran","year":"2010","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.fsi.2025.110877_bib116","article-title":"Virus-derived small RNAs in the penaeid shrimp Fenneropenaeus chinensis during acute infection of the DNA virus WSSV","volume":"6","author":"Liu","year":"2016","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fsi.2025.110877_bib117","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1016\/j.jmb.2005.03.013","article-title":"Induction of inflammatory cytokines and interferon responses by double-stranded and single-stranded siRNAs is sequence-dependent and requires endosomal localization","volume":"348","author":"Sioud","year":"2005","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.fsi.2025.110877_bib118","doi-asserted-by":"crossref","first-page":"4814","DOI":"10.1111\/j.1742-4658.2010.07904.x","article-title":"Efficient and targeted delivery system of siRNA in vivo","volume":"277","author":"Shim","year":"2010","journal-title":"FEBS J."},{"key":"10.1016\/j.fsi.2025.110877_bib119","doi-asserted-by":"crossref","first-page":"W124","DOI":"10.1093\/nar\/gkh442","article-title":"siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference","volume":"32","author":"Naito","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.fsi.2025.110877_bib120","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1104\/pp.106.083295","article-title":"Computational estimation and experimental verification of off-target silencing during posttranscriptional gene silencing in plants","volume":"142","author":"Xu","year":"2006","journal-title":"Plant Physiol"},{"key":"10.1016\/j.fsi.2025.110877_bib121","doi-asserted-by":"crossref","first-page":"6927","DOI":"10.1093\/nar\/gkp735","article-title":"Off-target and a portion of target-specific siRNA mediated mRNA degradation is Ago2 \u2018Slicer\u2019 independent and can be mediated by Ago1","volume":"37","author":"Vickers","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.fsi.2025.110877_bib122","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1038\/nm1191","article-title":"Sequence-specific potent induction of IFN-\u03b1 by short interfering RNA in plasmacytoid dendritic cells through TLR7","volume":"11","author":"Hornung","year":"2005","journal-title":"Nat. Med."},{"key":"10.1016\/j.fsi.2025.110877_bib123","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1111\/raq.12482","article-title":"Immune responses and immunoprotection in crustaceans with special reference to shrimp","volume":"13","author":"Kulkarni","year":"2021","journal-title":"Rev. Aquacult."},{"key":"10.1016\/j.fsi.2025.110877_bib124","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1111\/jfd.12279","article-title":"Recent insights into host\u2013pathogen interaction in white spot syndrome virus infected penaeid shrimp","volume":"38","author":"Shekhar","year":"2015","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2025.110877_bib125","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.yrtph.2016.07.009","article-title":"Corn rootworm-active RNA DvSnf7: repeat dose oral toxicology assessment in support of human and mammalian safety","volume":"81","author":"Petrick","year":"2016","journal-title":"Regul. Toxicol. Pharmacol."},{"key":"10.1016\/j.fsi.2025.110877_bib126","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1261\/rna.7272305","article-title":"The contributions of dsRNA structure to Dicer specificity and efficiency","volume":"11","author":"Vermeulen","year":"2005","journal-title":"RNA"},{"key":"10.1016\/j.fsi.2025.110877_bib127","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.virusres.2010.09.009","article-title":"Double-stranded RNA confers both preventive and therapeutic effects against Penaeus stylirostris densovirus (PstDNV) in Litopenaeus vannamei","volume":"155","author":"Ho","year":"2011","journal-title":"Virus Res."},{"key":"10.1016\/j.fsi.2025.110877_bib128","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.jviromet.2012.11.033","article-title":"A simple one-step method for producing dsRNA from E. coli to inhibit shrimp virus replication","volume":"188","author":"Posiri","year":"2013","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.fsi.2025.110877_bib129","series-title":"Plant Gene Silencing: Methods and Protocols","first-page":"211","article-title":"In vitro method for synthesis of large-scale dsRNA molecule as a novel plant protection strategy","author":"Sundaresha","year":"2022"},{"key":"10.1016\/j.fsi.2025.110877_bib130","doi-asserted-by":"crossref","first-page":"5515","DOI":"10.1073\/pnas.082117599","article-title":"A DNA vector-based RNAi technology to suppress gene expression in mammalian cells","volume":"99","author":"Sui","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.fsi.2025.110877_bib131","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1038\/nbt0502-505","article-title":"Effective expression of small interfering RNA in human cells","volume":"20","author":"Paul","year":"2002","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.fsi.2025.110877_bib132","doi-asserted-by":"crossref","first-page":"6223","DOI":"10.1093\/nar\/13.17.6223","article-title":"A novel transcription property of SP6 and T7 RNA polymerases: dependence on template structure","volume":"13","author":"Schenborn","year":"1985","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.fsi.2025.110877_bib133","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1093\/protein\/1.1.67","article-title":"Single-stranded DNA \u2018blue\u2019 T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering","volume":"1","author":"Mead","year":"1986","journal-title":"Protein Eng. Des. Sel."},{"key":"10.1016\/j.fsi.2025.110877_bib134","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2022.1017934","article-title":"RNA sensor response in HeLa cells for transfected mRNAs prepared in vitro by SP6 and HiT7 RNA polymerases: a comparative study","volume":"10","author":"Nagaraj","year":"2022","journal-title":"Front. Bioeng. Biotechnol."},{"issue":"2","key":"10.1016\/j.fsi.2025.110877_bib135","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1158\/1078-0432.565.11.2","article-title":"Interlaboratory comparability study of cancer gene expression analysis using oligonucleotide microarrays","volume":"11","author":"Dobbin","year":"2005","journal-title":"Clin. Cancer Res."},{"key":"10.1016\/j.fsi.2025.110877_bib136","doi-asserted-by":"crossref","DOI":"10.3389\/fmolb.2024.1504876","article-title":"Bacteriophage RNA polymerases: catalysts for mRNA vaccines and therapeutics","volume":"11","author":"Nair","year":"2024","journal-title":"Front. Mol. Biosci."},{"key":"10.1016\/j.fsi.2025.110877_bib137","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s13036-023-00323-1","article-title":"T7Max transcription system","volume":"17","author":"Deich","year":"2023","journal-title":"J. Biol. Eng."},{"key":"10.1016\/j.fsi.2025.110877_bib138","first-page":"2099","article-title":"Bacterial delivery of RNAi effectors: transkingdom RNAi","volume":"42","author":"Lage","year":"2010","journal-title":"J. Vis. Exp."},{"issue":"11","key":"10.1016\/j.fsi.2025.110877_bib139","doi-asserted-by":"crossref","first-page":"3415","DOI":"10.1002\/bit.28806","article-title":"Quality by design approach to improve quality and decrease cost of in vitro transcription of mRNA using design of experiments","volume":"121","author":"Boman","year":"2024","journal-title":"Biotechnol. Bioeng."},{"key":"10.1016\/j.fsi.2025.110877_bib140","doi-asserted-by":"crossref","DOI":"10.3389\/fmolb.2023.1291045","article-title":"Formation of dsRNA by-products during in Vitro transcription can be reduced by using low steady-state levels of UTP","volume":"10","author":"Ziegenhals","year":"2023","journal-title":"Front. Mol. Biosci."},{"issue":"26","key":"10.1016\/j.fsi.2025.110877_bib141","doi-asserted-by":"crossref","DOI":"10.1002\/ange.202305373","article-title":"Insertion of chemical handles into the backbone of DNA during solid\u2010phase synthesis by oxidative coupling of amines to phosphites","volume":"135","author":"Hansen","year":"2023","journal-title":"Angew. Chem."},{"key":"10.1016\/j.fsi.2025.110877_bib142","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2022.980592","article-title":"Strategies for the production of dsRNA biocontrols as alternatives to chemical pesticides","volume":"10","author":"Hough","year":"2022","journal-title":"Front. Bioeng. Biotechnol."},{"issue":"13","key":"10.1016\/j.fsi.2025.110877_bib143","doi-asserted-by":"crossref","first-page":"2265","DOI":"10.1002\/1521-3773(20020703)41:13<2265::AID-ANIE2265>3.0.CO;2-3","article-title":"Small interfering RNAs and their chemical synthesis","volume":"41","author":"Micura","year":"2002","journal-title":"Angew. Chem. Int. Ed."},{"issue":"5","key":"10.1016\/j.fsi.2025.110877_bib144","doi-asserted-by":"crossref","first-page":"2726","DOI":"10.1021\/acs.joc.2c02195","article-title":"Expansion of phosphoramidite chemistry in solid-phase oligonucleotide synthesis: rapid 3\u2032-dephosphorylation and strand cleavage","volume":"88","author":"Yamamoto","year":"2023","journal-title":"J. Org. Chem."},{"issue":"6","key":"10.1016\/j.fsi.2025.110877_bib145","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1021\/bc800406a","article-title":"Solid-phase synthesis of oligonucleotide conjugates useful for delivery and targeting of potential nucleic acid therapeutics","volume":"20","author":"L\u00f6nnberg","year":"2009","journal-title":"Bioconjug. Chem."},{"key":"10.1016\/j.fsi.2025.110877_bib146","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1038\/s41392-022-01272-9","article-title":"RNA interfering machinery: new insight into the Dicer-2 complexes in the dsRNA-processing cycle","volume":"8","author":"Wei","year":"2023","journal-title":"Signal Transduct. Targeted Ther."},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib147","doi-asserted-by":"crossref","first-page":"4268","DOI":"10.1038\/s41467-021-24555-1","article-title":"Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2","volume":"12","author":"Naganuma","year":"2021","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib148","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.virusres.2012.11.012","article-title":"Ingestion of bacteria expressing dsRNA triggers specific RNA silencing in shrimp","volume":"171","author":"Attasart","year":"2013","journal-title":"Virus Res."},{"key":"10.1016\/j.fsi.2025.110877_bib149","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.jbiotec.2014.03.016","article-title":"Protection of yellow head virus infection in shrimp by feeding of bacteria expressing dsRNAs","volume":"179","author":"Sanitt","year":"2014","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.fsi.2025.110877_bib150","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.jip.2016.01.003","article-title":"A formulated double-stranded RNA diet for reducing Penaeus monodon densovirus infection in black tiger shrimp","volume":"134","author":"Chimwai","year":"2016","journal-title":"J. Invertebr. Pathol."},{"key":"10.1016\/j.fsi.2025.110877_bib151","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2024.129840","article-title":"Oral delivery of bacteria expressing wsv108 gene-specific dsRNA protects shrimp from white spot syndrome virus (WSSV) infection","volume":"261","author":"Yang","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.fsi.2025.110877_bib152","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2021.737557","article-title":"Systemic gene silencing from oral uptake of dsRNA in Litopenaeus vannamei requires both clathrin-mediated endocytosis and LvSID-1","volume":"548","author":"Maruekawong","year":"2022","journal-title":"Aquaculture"},{"issue":"4","key":"10.1016\/j.fsi.2025.110877_bib153","doi-asserted-by":"crossref","first-page":"2057","DOI":"10.1128\/JVI.78.4.2057-2061.2004","article-title":"Protection of Penaeus monodon against white spot syndrome virus by oral vaccination","volume":"78","author":"Witteveldt","year":"2004","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2025.110877_bib154","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2021.736834","article-title":"Bacillus subtilis expressing double-strand RNAs (dsRNAs) induces RNA interference mechanism (RNAi) and increases survival of WSSV-challenged Litopenaeus vannamei","volume":"541","author":"Riet","year":"2021","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110877_bib155","article-title":"Probiotics expressing double-stranded RNA targeting vp28 efficiently protect shrimps from WSSV infection","volume":"23","author":"Dekham","year":"2022","journal-title":"Aquac. Rep."},{"issue":"4","key":"10.1016\/j.fsi.2025.110877_bib156","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1111\/raq.12916","article-title":"Nanoparticles\u2010based double\u2010stranded RNA delivery as an antiviral agent in shrimp aquaculture","volume":"16","author":"Jonjaroen","year":"2024","journal-title":"Rev. Aquacult."},{"issue":"8","key":"10.1016\/j.fsi.2025.110877_bib157","doi-asserted-by":"crossref","first-page":"1893","DOI":"10.3390\/microorganisms11081893","article-title":"Transgenic microalgae expressing double-stranded RNA as potential feed supplements for controlling white spot syndrome in shrimp aquaculture","volume":"11","author":"Charoonnart","year":"2023","journal-title":"Microorganisms"},{"key":"10.1016\/j.fsi.2025.110877_bib158","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.aqrep.2016.03.003","article-title":"Use of microalgae Chlamydomonas reinhardtii for production of double-stranded RNA against shrimp virus","volume":"3","author":"Somchai","year":"2016","journal-title":"Aquac. Rep."},{"key":"10.1016\/j.fsi.2025.110877_bib159","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1111\/are.13479","article-title":"Production of specific dsRNA against white spot syndrome virus in the yeast Yarrowia lipolytica","volume":"49","author":"Alvarez-Sanchez","year":"2017","journal-title":"Aquac. Res."},{"key":"10.1016\/j.fsi.2025.110877_bib160","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.aquaculture.2014.09.034","article-title":"Delivery of double stranded RNA by Macrobrachium rosenbergii nodavirus-like particles to protect shrimp from white spot syndrome virus","volume":"435","author":"Jariyapong","year":"2015","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110877_bib161","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.aquaculture.2015.05.016","article-title":"Enhancement of shrimp immunity against white spot syndrome virus by Macrobrachium rosenbergii nodavirus-like particle encapsulated vp28 double-stranded RNA","volume":"446","author":"Jariyapong","year":"2015","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110877_bib162","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.aquaculture.2019.02.001","article-title":"Infectious hypodermal and hematopoietic necrosis virus-like particles encapsulating vp28 double-stranded RNA protect shrimp from white spot syndrome virus","volume":"504","author":"Jariyapong","year":"2019","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110877_bib163","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.fsi.2021.03.024","article-title":"Dual vp28 and vp37 dsRNA encapsulation in IHHNV virus-like particles enhances shrimp protection against white spot syndrome virus","volume":"113","author":"Weerachatyanukul","year":"2021","journal-title":"Fish Shellfish Immunol."},{"issue":"12","key":"10.1016\/j.fsi.2025.110877_bib164","doi-asserted-by":"crossref","first-page":"2594","DOI":"10.3390\/v14122594","article-title":"Simultaneous production of a virus-like particle linked to dsRNA to enhance dsRNA delivery for yellow head virus inhibition","volume":"14","author":"Worawittayatada","year":"2022","journal-title":"Viruses"},{"key":"10.1016\/j.fsi.2025.110877_bib165","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.jbiotec.2018.01.002","article-title":"Administration of co-expressed Penaeus stylirostris densovirus-like particles and dsRNA-YHV-Pro provide protection against yellow head virus in shrimp","volume":"267","author":"Sinnuengnong","year":"2018","journal-title":"J. Biotechnol."},{"key":"10.1016\/j.fsi.2025.110877_bib166","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.3762\/bjoc.17.95","volume":"17","author":"Ramos-Carre\u00f1o","year":"2021","journal-title":"Beilstein J. Org. Chem."},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib167","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1039\/C7ME00092H","article-title":"Anti-viral RNAi nanoparticles protect shrimp against white spot disease","volume":"3","author":"Ufaz","year":"2018","journal-title":"Mol. Syst. Des. Eng."},{"issue":"3","key":"10.1016\/j.fsi.2025.110877_bib168","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1007\/s10126-007-9057-6","article-title":"Oral administration of bacterially expressed vp28 dsRNA to protect Penaeus monodon from white spot syndrome virus","volume":"10","author":"Sarathi","year":"2008","journal-title":"Mar. Biotechnol."},{"issue":"9","key":"10.1016\/j.fsi.2025.110877_bib169","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.3390\/vaccines10091428","article-title":"RNA nanovaccine protects against white spot syndrome virus in shrimp","volume":"10","author":"Phanse","year":"2022","journal-title":"Vaccines"},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib170","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1111\/j.1365-2109.2012.03209.x","article-title":"Highly efficient double-stranded RNA transfection of penaeid shrimp using cationic liposomes","volume":"45","author":"Apiratikul","year":"2013","journal-title":"Aquac. Res."},{"key":"10.1016\/j.fsi.2025.110877_bib171","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.jbiotec.2016.04.049","article-title":"Cholesterol-based cationic liposome increases dsRNA protection of yellow head virus infection in Penaeus vannamei","volume":"228","author":"Sanitt","year":"2016","journal-title":"J. Biotechnol."},{"issue":"2","key":"10.1016\/j.fsi.2025.110877_bib172","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.jconrel.2011.11.030","article-title":"Alkane-modified short polyethyleneimine for siRNA delivery","volume":"160","author":"Schroeder","year":"2012","journal-title":"J. Control. Release"},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib173","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3354\/dao02343","article-title":"Penaeus monodon is protected against gill-associated virus by muscle injection but not oral delivery of bacterially expressed dsRNAs","volume":"95","author":"Sellars","year":"2011","journal-title":"Dis. Aquat. Org."},{"issue":"5","key":"10.1016\/j.fsi.2025.110877_bib174","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1007\/s10499-017-0144-z","article-title":"Probiotic bacteria (Lactobacillus plantarum) expressing specific double-stranded RNA and its potential for controlling shrimp viral and bacterial diseases","volume":"25","author":"Thammasorn","year":"2017","journal-title":"Aquac. Int."},{"key":"10.1016\/j.fsi.2025.110877_bib175","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2022.1066799","article-title":"Potential dsRNAs can be delivered to aquatic animals for defense against pathogens","volume":"10","author":"Nie","year":"2022","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.fsi.2025.110877_bib176","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.cofs.2020.02.007","article-title":"Pathogen subtyping tools for risk assessment and management of produce-borne outbreaks","volume":"32","author":"Alegbeleye","year":"2020","journal-title":"Curr. Opin. Food Sci."},{"key":"10.1016\/j.fsi.2025.110877_bib177","article-title":"Advances in nanocarriers to improve the stability of dsRNA in the environment","volume":"10","author":"Yang","year":"2022","journal-title":"Front. Bioeng. Biotechnol."},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib178","doi-asserted-by":"crossref","first-page":"19","DOI":"10.2306\/scienceasia1513-1874.2020.S003","article-title":"Bacillus subtilis expressing dsvp28 improved shrimp survival from WSSV challenge","volume":"46S","author":"Saelim","year":"2020","journal-title":"Sci. Asia"},{"key":"10.1016\/j.fsi.2025.110877_bib179","doi-asserted-by":"crossref","DOI":"10.1016\/j.copbio.2022.102784","article-title":"Production of recombinant and therapeutic proteins in microalgae","volume":"78","author":"Banerjee","year":"2022","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.fsi.2025.110877_bib180","doi-asserted-by":"crossref","first-page":"279","DOI":"10.3389\/fpls.2020.00279","article-title":"Emerging technologies in algal biotechnology: toward the establishment of a sustainable algae-based economy","volume":"11","author":"Fabris","year":"2020","journal-title":"Front. Plant Sci."},{"issue":"12","key":"10.1016\/j.fsi.2025.110877_bib181","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1111\/j.1461-0248.2007.01113.x","article-title":"Global analysis of nitrogen and phosphorus limitation of primary producers in freshwater, marine and terrestrial ecosystems","volume":"10","author":"Elser","year":"2007","journal-title":"Ecol. Lett."},{"issue":"2","key":"10.1016\/j.fsi.2025.110877_bib182","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.biotechadv.2006.11.002","article-title":"Micro-algae as a source of protein","volume":"25","author":"Becker","year":"2007","journal-title":"Biotechnol. Adv."},{"issue":"3","key":"10.1016\/j.fsi.2025.110877_bib183","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.biotechadv.2007.02.001","article-title":"Biodiesel from microalgae","volume":"25","author":"Chisti","year":"2007","journal-title":"Biotechnol. Adv."},{"issue":"3","key":"10.1016\/j.fsi.2025.110877_bib184","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1080\/07388550500248571","article-title":"Use of algae for removing heavy metal ions from wastewater: progress and prospects","volume":"25","author":"Mehta","year":"2005","journal-title":"Crit. Rev. Biotechnol."},{"issue":"3","key":"10.1016\/j.fsi.2025.110877_bib185","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1080\/07388550500248498","article-title":"Bioactive compounds from Cyanobacteria and microalgae: an overview","volume":"25","author":"Singh","year":"2005","journal-title":"Crit. Rev. Biotechnol."},{"issue":"3","key":"10.1016\/j.fsi.2025.110877_bib186","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1023\/A:1008106304417","article-title":"The role of microalgae in aquaculture: situation and trends","volume":"12","author":"Muller-Feuga","year":"2000","journal-title":"J. Appl. Phycol."},{"issue":"4","key":"10.1016\/j.fsi.2025.110877_bib187","doi-asserted-by":"crossref","first-page":"743","DOI":"10.3390\/microorganisms10040743","article-title":"Harnessing the algal chloroplast for heterologous protein production","volume":"10","author":"Cutolo","year":"2022","journal-title":"Microorganisms"},{"key":"10.1016\/j.fsi.2025.110877_bib188","doi-asserted-by":"crossref","first-page":"914","DOI":"10.3389\/fbioe.2020.00914","article-title":"Bioengineering of microalgae: recent advances, perspective, and regulatory challenges for industrial application","volume":"8","author":"Kumar","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib189","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1111\/raq.12322","article-title":"Advances and challenges in genetic engineering of microalgae","volume":"12","author":"Fajardo","year":"2019","journal-title":"Rev. Aquacult."},{"issue":"12","key":"10.1016\/j.fsi.2025.110877_bib190","doi-asserted-by":"crossref","first-page":"5467","DOI":"10.1007\/s00253-016-7354-6","article-title":"New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii","volume":"100","author":"Wannathong","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"4","key":"10.1016\/j.fsi.2025.110877_bib191","doi-asserted-by":"crossref","first-page":"46","DOI":"10.3390\/biology7040046","article-title":"Selectable markers and reporter genes for engineering the chloroplast of Chlamydomonas reinhardtii","volume":"7","author":"Esland","year":"2018","journal-title":"Biology"},{"key":"10.1016\/j.fsi.2025.110877_bib192","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.fsi.2017.08.036","article-title":"Marine yeast Yarrowia lipolytica improves the immune responses in Pacific Red Snapper (Lutjanus Peru) leukocytes","volume":"70","author":"Alamillo","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110877_bib193","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.fsi.2020.07.043","article-title":"Dietary supplementation of marine yeast Yarrowia lipolytica modulates immune response in Litopenaeus vannamei","volume":"105","author":"Licona-Jain","year":"2020","journal-title":"Fish Shellfish Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib194","doi-asserted-by":"crossref","first-page":"123","DOI":"10.2174\/138945013804806523","article-title":"Colloidal drug delivery systems in vaccine delivery","volume":"14","author":"Beg","year":"2013","journal-title":"Curr. Drug Targets"},{"issue":"4","key":"10.1016\/j.fsi.2025.110877_bib195","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1007\/s00705-009-0336-6","article-title":"Expression and self-assembly of virus-like particles of infectious hypodermal and hematopoietic necrosis virus in Escherichia coli","volume":"154","author":"Hou","year":"2009","journal-title":"Arch. Virol."},{"issue":"12","key":"10.1016\/j.fsi.2025.110877_bib196","doi-asserted-by":"crossref","first-page":"2594","DOI":"10.3390\/v14122594","article-title":"Simultaneous production of a virus-like particle linked to dsRNA to enhance dsRNA delivery for yellow head virus inhibition","volume":"14","author":"Worawittayatada","year":"2022","journal-title":"Viruses"},{"issue":"8","key":"10.1016\/j.fsi.2025.110877_bib197","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1038\/nnano.2014.84","article-title":"In vivo endothelial siRNA delivery using polymeric nanoparticles with low molecular weight","volume":"9","author":"Dahlman","year":"2014","journal-title":"Nat. Nanotechnol."},{"issue":"7","key":"10.1016\/j.fsi.2025.110877_bib198","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1016\/j.progpolymsci.2006.06.001","article-title":"Chitin and chitosan: properties and applications","volume":"31","author":"Rinaudo","year":"2006","journal-title":"Prog. Polym. Sci."},{"issue":"4","key":"10.1016\/j.fsi.2025.110877_bib199","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1002\/jbm.a.30545","article-title":"Single dose vaccine based on biodegradable polyanhydride microspheres can modulate immune response mechanism","volume":"76A","author":"Kipper","year":"2006","journal-title":"J. Biomed. Mater. Res."},{"issue":"11","key":"10.1016\/j.fsi.2025.110877_bib200","doi-asserted-by":"crossref","first-page":"4161","DOI":"10.1002\/jbm.a.35086","article-title":"Structural and antigenic stability of H5N1 hemagglutinin trimer upon release from polyanhydride nanoparticles: stability of hemagglutinin trimer","volume":"102","author":"Ross","year":"2014","journal-title":"J. Biomed. Mater. Res."},{"issue":"5","key":"10.1016\/j.fsi.2025.110877_bib201","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1002\/jgm.696","article-title":"Exploring polyethylenimine\u2010mediated DNA transfection and the proton sponge hypothesis","volume":"7","author":"Akinc","year":"2005","journal-title":"J. Gene Med."},{"issue":"1\u20134","key":"10.1016\/j.fsi.2025.110877_bib202","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.aquaculture.2004.09.037","article-title":"Evaluation of methods for DNA delivery into shrimp zygotes of Penaeus (Litopenaeus) vannamei","volume":"243","author":"Sun","year":"2005","journal-title":"Aquaculture"},{"issue":"9","key":"10.1016\/j.fsi.2025.110877_bib203","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/S1369-7021(12)70165-7","article-title":"PDMAEMA based gene delivery materials","volume":"15","author":"Agarwal","year":"2012","journal-title":"Mater. Today"},{"issue":"2","key":"10.1016\/j.fsi.2025.110877_bib204","doi-asserted-by":"crossref","first-page":"63","DOI":"10.3390\/fishes9020063","article-title":"Use of algae in aquaculture: a review","volume":"9","author":"Vijayaram","year":"2024","journal-title":"Fishes"},{"issue":"5","key":"10.1016\/j.fsi.2025.110877_bib205","doi-asserted-by":"crossref","first-page":"59","DOI":"10.7324\/JABB.2022.100507","article-title":"Use of yeasts in aquaculture nutrition and immunostimulation: a review","volume":"10","author":"Mahdy","year":"2022","journal-title":"J. Appl. Biol. Biotechnol."},{"issue":"1","key":"10.1016\/j.fsi.2025.110877_bib206","article-title":"Performance of selected transgenic traits against western corn rootworm, 2022","volume":"50","author":"Lyons","year":"2025","journal-title":"Arthropod Manag. Tests"},{"issue":"7","key":"10.1016\/j.fsi.2025.110877_bib207","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0288372","article-title":"Effects of SmartStax\u00ae and SmartStax\u00ae PRO maize on western corn rootworm (Diabrotica virgifera virgifera LeConte) larval feeding injury and adult life history parameters","volume":"18","author":"Reinders","year":"2023","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2025.110877_bib208","series-title":"RNA Interference in Agriculture: Basic Science to Applications: from Bioinformatics and Laboratory Assays over Regulatory Issues to Field Uses","first-page":"679","article-title":"Calantha\u2122: the first commercialized sprayable dsRNA product for insect control","author":"Narva","year":"2025"},{"key":"10.1016\/j.fsi.2025.110877_bib209","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.3390\/biom14111358","article-title":"RNA interference applied to crustacean aquaculture","volume":"14","author":"Fajardo","year":"2024","journal-title":"Biomolecules"},{"issue":"6","key":"10.1016\/j.fsi.2025.110877_bib210","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1002\/ps.4813","article-title":"RNA interference technology in crop protection against arthropod pests, pathogens and nematodes","volume":"74","author":"Zotti","year":"2018","journal-title":"Pest Manag. Sci."},{"key":"10.1016\/j.fsi.2025.110877_bib211","doi-asserted-by":"crossref","first-page":"21","DOI":"10.3389\/fpls.2020.00021","article-title":"Environmental fate and dissipation of applied dsRNA in soil, aquatic systems, and plants","volume":"11","author":"Bachman","year":"2020","journal-title":"Front. Plant Sci."},{"issue":"3","key":"10.1016\/j.fsi.2025.110877_bib212","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1002\/etc.3585","article-title":"Aquatic fate of a double-stranded RNA in a sediment\u2013water system following an over-water application","volume":"36","author":"Fischer","year":"2017","journal-title":"Environ. Toxicol. Chem."},{"key":"10.1016\/j.fsi.2025.110877_bib213","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2022.836106","article-title":"Methods for the cost-effective production of bacteria-derived double-stranded RNA for in vitro knockdown studies","volume":"13","author":"Verdonckt","year":"2022","journal-title":"Front. Physiol."},{"key":"10.1016\/j.fsi.2025.110877_bib214","series-title":"U.S. Patent No. 11,142,768","author":"Rodrigues","year":"2021"},{"issue":"SI","key":"10.1016\/j.fsi.2025.110877_bib215","doi-asserted-by":"crossref","first-page":"102","DOI":"10.2112\/SI86-015.1","article-title":"Determining the dosage and time of injection for WSSV vp28 double stranded RNA to Penaeus indicus in providing effective protection against WSSV","volume":"86","author":"Amarnath","year":"2019","journal-title":"J. Coast Res."},{"issue":"1\u20134","key":"10.1016\/j.fsi.2025.110877_bib216","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/S0044-8486(02)00509-4","article-title":"Nutrients, phytoplankton and harmful algal blooms in shrimp ponds: a review with special reference to the situation in the Gulf of California","volume":"219","author":"Alonso-Rodr\u00edguez","year":"2003","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.110877_bib217","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/j.fsi.2020.04.036","article-title":"Effects of dietary yeast culture on shrimp growth, immune response, intestinal health and disease resistance against Vibrio harveyi","volume":"102","author":"Ayiku","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.110877_bib218","first-page":"64","article-title":"Fungus (Fusarium) disease of juvenile and adult penaeid shrimp","volume":"17","author":"Lightner","year":"1988"},{"key":"10.1016\/j.fsi.2025.110877_bib219","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2021.737602","article-title":"Identification of Fusarium solani as a causal agent of black spot disease (BSD) of Pacific white shrimp, Penaeus vannamei","volume":"548","author":"Yao","year":"2022","journal-title":"Aquaculture"},{"issue":"13","key":"10.1016\/j.fsi.2025.110877_bib220","doi-asserted-by":"crossref","first-page":"7124","DOI":"10.1039\/C4NR00915K","article-title":"Functionalization of protein-based nanocages for drug delivery applications","volume":"6","author":"Schoonen","year":"2014","journal-title":"Nanoscale"},{"issue":"6017","key":"10.1016\/j.fsi.2025.110877_bib221","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1126\/science.1199081","article-title":"Directed evolution of a protein container","volume":"331","author":"Worsdorfer","year":"2011","journal-title":"Science"},{"issue":"10","key":"10.1016\/j.fsi.2025.110877_bib222","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1005203","article-title":"Crystal structures of a piscine betanodavirus: mechanisms of capsid assembly and viral infection","volume":"11","author":"Chen","year":"2015","journal-title":"PLoS Pathog."}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464825007661?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464825007661?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T21:14:42Z","timestamp":1779916482000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464825007661"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":222,"alternative-id":["S1050464825007661"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2025.110877","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A comparative review of RNAi delivery systems for shrimp aquaculture and future directions","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2025.110877","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"110877"}}