{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T02:19:09Z","timestamp":1773368349471,"version":"3.50.1"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100002701","name":"Korea Ministry of Education","doi-asserted-by":"publisher","award":["RS-2023-0027588830782064780003"],"award-info":[{"award-number":["RS-2023-0027588830782064780003"]}],"id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish &amp; Shellfish Immunology"],"published-print":{"date-parts":[[2026,1]]},"DOI":"10.1016\/j.fsi.2025.111000","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T11:47:55Z","timestamp":1763034475000},"page":"111000","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Protective efficacy of miR-155 encapsulated using MS2 bacteriophage-based virus-like particle (MS2-VLP) against viral hemorrhagic septicemia virus (VHSV) in olive flounder"],"prefix":"10.1016","volume":"168","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5312-737X","authenticated-orcid":false,"given":"So Yeon","family":"Kim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeo Jin","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5935-0249","authenticated-orcid":false,"given":"Min Sun","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"6","key":"10.1016\/j.fsi.2025.111000_bib1","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1016\/j.jhep.2011.11.026","article-title":"Diagnostic and therapeutic potential of miRNA signatures in patients with hepatocellular carcinoma","volume":"56","author":"Borel","year":"2012","journal-title":"J. Hepatol."},{"key":"10.1016\/j.fsi.2025.111000_bib2","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/j.fsi.2017.02.031","article-title":"Characterization of microRNAs in orange-spotted grouper (Epinephelus coioides) fin cells upon red-spotted grouper nervous necrosis virus infection","volume":"63","author":"Chen","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.111000_bib3","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1186\/s12864-016-2505-9","article-title":"Characterization of an extensive rainbow trout miRNA transcriptome by next-generation sequencing","volume":"17","author":"Juanchich","year":"2016","journal-title":"BMC Genom."},{"key":"10.1016\/j.fsi.2025.111000_bib4","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.fsi.2016.03.014","article-title":"Changes in the microRNAs expression profile of olive flounder (Paralichthys olivaceus) in response to viral hemorrhagic septicemia virus (VHSV) infection","volume":"51","author":"Najib","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.111000_bib5","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1007\/s11160-018-9524-3","article-title":"Infectious disease in fish: global risk of viral hemorrhagic septicemia virus","volume":"28","author":"Escobar","year":"2018","journal-title":"Rev. Fish Biol. Fish."},{"issue":"1","key":"10.1016\/j.fsi.2025.111000_bib6","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1023\/A:1008140707132","article-title":"Complete genomic sequence of viral hemorrhagic septicemia virus, a fish rhabdovirus","volume":"19","author":"Sch\u00fctze","year":"1999","journal-title":"Virus Genes"},{"issue":"28","key":"10.1016\/j.fsi.2025.111000_bib7","doi-asserted-by":"crossref","first-page":"3215","DOI":"10.1016\/j.vaccine.2015.04.092","article-title":"Involvement of two microRNAs in the early immune response to DNA vaccination against a fish rhabdovirus","volume":"33","author":"Bela-ong","year":"2015","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2025.111000_bib8","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.fsi.2019.02.056","article-title":"Effect of miR-155 as a molecular adjuvant of DNA vaccine against VHSV in olive flounder (Paralichthys olivaceus)","volume":"88","author":"Lim","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.111000_bib9","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.fsi.2019.09.026","article-title":"Generation of microRNA-30e-producing recombinant viral hemorrhagic septicemia virus (VHSV) and its effect on in vitro immune responses","volume":"94","author":"Kwak","year":"2019","journal-title":"Fish Shellfish Immunol."},{"issue":"4","key":"10.1016\/j.fsi.2025.111000_bib10","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1208\/s12248-010-9210-4","article-title":"Delivery of siRNA therapeutics: barriers and carriers","volume":"12","author":"Wang","year":"2010","journal-title":"AAPS J."},{"key":"10.1016\/j.fsi.2025.111000_bib11","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1038\/gt.2015.31","article-title":"Adenovirus-mediated artificial MicroRNAs targeting matrix or nucleoprotein genes protect mice against lethal influenza virus challenge","volume":"22","author":"Zhang","year":"2015","journal-title":"Gene Ther."},{"issue":"2","key":"10.1016\/j.fsi.2025.111000_bib12","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1038\/gene.2011.58","article-title":"Systemic in vivo lentiviral delivery of miR-15a\/16 reduces malignancy in the NZB de novo mouse model of chronic lymphocytic leukemia","volume":"13","author":"Kasar","year":"2012","journal-title":"Gene Immun."},{"issue":"11","key":"10.1016\/j.fsi.2025.111000_bib13","doi-asserted-by":"crossref","first-page":"5500","DOI":"10.4049\/jimmunol.1103505","article-title":"MicroRNA-494 is required for the accumulation and functions of tumor-expanded myeloid-derived suppressor cells via targeting of PTEN","volume":"188","author":"Liu","year":"2012","journal-title":"J. Immunol."},{"issue":"6","key":"10.1016\/j.fsi.2025.111000_bib14","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1038\/mt.2012.67","article-title":"Lipid-based nanoparticle delivery of Pre-miR-107 inhibits the tumorigenicity of head and neck squamous cell carcinoma","volume":"20","author":"Piao","year":"2012","journal-title":"Mol. Ther."},{"issue":"15","key":"10.1016\/j.fsi.2025.111000_bib15","doi-asserted-by":"crossref","first-page":"5214","DOI":"10.1158\/0008-5472.CAN-10-4645","article-title":"MicroRNA replacement therapy for miR-145 and miR-33a is efficacious in a model of colon carcinoma","volume":"71","author":"Ibrahim","year":"2011","journal-title":"Cancer Res."},{"issue":"11","key":"10.1016\/j.fsi.2025.111000_bib16","doi-asserted-by":"crossref","first-page":"3097","DOI":"10.3390\/cells10113097","article-title":"The challenges and opportunities in the development of microRNA therapeutics: a multidisciplinary viewpoint","volume":"10","author":"Momin","year":"2021","journal-title":"Cells"},{"issue":"7","key":"10.1016\/j.fsi.2025.111000_bib17","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1111\/j.1742-4658.2012.08512.x","article-title":"Development of a microRNA delivery system based on bacteriophage MS2 virus-like particles","volume":"279","author":"Pan","year":"2012","journal-title":"FEBS J."},{"issue":"11","key":"10.1016\/j.fsi.2025.111000_bib18","doi-asserted-by":"crossref","first-page":"1776","DOI":"10.1261\/rna.7710304","article-title":"The crystal structure of a high-affinity RNA stem-loop complexed with the bacteriophage MS2 capsid: further challenges in the modeling of ligand-RNA interactions","volume":"10","author":"Horn","year":"2004","journal-title":"RNA"},{"issue":"19","key":"10.1016\/j.fsi.2025.111000_bib19","doi-asserted-by":"crossref","first-page":"4621","DOI":"10.1093\/nar\/21.19.4621","article-title":"Encapsidation of heterologous RNAs by bacteriophage MS2 coat protein","volume":"21","author":"Pickett","year":"1993","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.fsi.2025.111000_bib20","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-021-98706-1","article-title":"Optimizing the synthesis and purification of MS2 virus like particles","volume":"11","author":"Hashemi","year":"2021","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fsi.2025.111000_bib21","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1477-3155-9-22","article-title":"Thermal stability of RNA phage virus-like particles displaying foreign peptides","volume":"9","author":"Caldeira","year":"2011","journal-title":"J. Nanobiotechnol."},{"issue":"7","key":"10.1016\/j.fsi.2025.111000_bib22","doi-asserted-by":"crossref","first-page":"5729","DOI":"10.1021\/nn201397z","article-title":"Cell-specific delivery of diverse cargos by bacteriophage MS2 virus-like particles","volume":"5","author":"Ashley","year":"2011","journal-title":"ACS Nano"},{"key":"10.1016\/j.fsi.2025.111000_bib23","doi-asserted-by":"crossref","first-page":"2801","DOI":"10.3389\/fimmu.2018.02801","article-title":"Non-coding RNAs function as immune regulators in teleost fish","volume":"9","author":"Wang","year":"2018","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2025.111000_bib24","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.dci.2017.02.023","article-title":"miRNAs associated with immune response in teleost fish","volume":"75","author":"Andreassen","year":"2017","journal-title":"Dev. Comp. Immunol."},{"issue":"8","key":"10.1016\/j.fsi.2025.111000_bib25","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1093\/gbe\/evu151","article-title":"MicroRNA in teleost fish","volume":"6","author":"Bizuayehu","year":"2014","journal-title":"Genome Biol. Evol."},{"key":"10.1016\/j.fsi.2025.111000_bib26","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.fsi.2018.11.030","article-title":"MicroRNA-155 promotes pro-inflammatory functions and augments apoptosis of monocytes\/macrophages during Vibrio anguillarum infection in ayu, Plecoglossus altivelis","volume":"86","author":"Nie","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.111000_bib27","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1186\/1471-2164-14-696","article-title":"MicroRNA-146 function in the innate immune transcriptome response of zebrafish embryos to Salmonella typhimurium infection","volume":"14","author":"Ordas","year":"2013","journal-title":"BMC Genom."},{"key":"10.1016\/j.fsi.2025.111000_bib28","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.molimm.2017.01.016","article-title":"MicroRNA-21 contributes to suppress cytokines production by targeting TLR28 in teleost fish","volume":"83","author":"Bi","year":"2017","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2025.111000_bib29","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.fsi.2020.07.042","article-title":"The miR-155 regulates cytokines expression by SOSC1 signal pathways of fish in vitro and in vivo","volume":"106","author":"Tang","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.111000_bib30","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.108937","article-title":"Antiviral effect of miR-155 in Epithelioma papulosum cyprini (EPC) cells against viral hemorrhagic septicemia virus (VHSV) infection","volume":"140","author":"Abdellaoui","year":"2023","journal-title":"Fish Shellfish Immunol."},{"issue":"5","key":"10.1016\/j.fsi.2025.111000_bib31","doi-asserted-by":"crossref","first-page":"958","DOI":"10.3390\/genes14050958","article-title":"Japanese flounder pol-miR-155 is involved in Edwardsiella tarda infection via ATG3","volume":"14","author":"Zhang","year":"2023","journal-title":"Genes"},{"issue":"1","key":"10.1016\/j.fsi.2025.111000_bib32","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1186\/s13046-025-03391-x","article-title":"MicroRNA in cancer therapy: breakthroughs and challenges in early clinical applications","volume":"44","author":"Martino","year":"2025","journal-title":"J. Exp. Clin. Cancer Res."},{"issue":"1","key":"10.1016\/j.fsi.2025.111000_bib33","doi-asserted-by":"crossref","first-page":"10","DOI":"10.3390\/mps4010010","article-title":"Recent advances in miRNA delivery systems","volume":"4","author":"Dasgupta","year":"2021","journal-title":"Methods Protoc."},{"issue":"15","key":"10.1016\/j.fsi.2025.111000_bib34","doi-asserted-by":"crossref","first-page":"2332","DOI":"10.3390\/cells11152332","article-title":"MicroRNAs and their big therapeutic impacts: delivery strategies for cancer intervention","volume":"11","author":"Holjencin","year":"2022","journal-title":"Cells"},{"key":"10.1016\/j.fsi.2025.111000_bib35","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s41544-019-0024-y","article-title":"Recent progress in microRNA-based delivery systems for the treatment of human disease","volume":"1","author":"Fu","year":"2019","journal-title":"ExRNA"},{"key":"10.1016\/j.fsi.2025.111000_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.109234","article-title":"Chitosan nanoparticles-based in vivo delivery of miR-155 modulates the viral haemorrhagic septicaemia virus-induced antiviral immune responses in zebrafish (Danio rerio)","volume":"144","author":"Liyanage","year":"2024","journal-title":"Fish Shellfish Immunol."},{"issue":"3","key":"10.1016\/j.fsi.2025.111000_bib37","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.jconrel.2006.08.017","article-title":"Accelerated blood clearance of PEGylated liposomes upon repeated injections: effect of doxorubicin-encapsulation and high-dose first injection","volume":"115","author":"Ishida","year":"2006","journal-title":"J. Contr. Release"},{"key":"10.1016\/j.fsi.2025.111000_bib38","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1038\/s41541-023-00766-z","article-title":"Polyethylene glycol (PEG)-associated immune responses triggered by clinically relevant lipid nanoparticles in rats","volume":"8","author":"Wang","year":"2023","journal-title":"npj Vaccines"},{"key":"10.1016\/j.fsi.2025.111000_bib39","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1159\/000141707","article-title":"Construction of armored RNA containing long-size chimeric RNA by increasing the number and affinity of the pac site in exogenous RNA and sequence coding coat protein of the MS2 bacteriophage","volume":"51","author":"Wei","year":"2008","journal-title":"Intervirology"},{"key":"10.1016\/j.fsi.2025.111000_bib40","doi-asserted-by":"crossref","first-page":"5957","DOI":"10.2147\/IJN.S37990","article-title":"MS2 VLP-based delivery of microRNA-146a inhibits autoantibody production in lupus-prone mice","volume":"7","author":"Pan","year":"2012","journal-title":"Int. J. Nanomed."},{"key":"10.1016\/j.fsi.2025.111000_bib41","first-page":"146","article-title":"MicroRNA-155 promotes the replication of Japanese encephalitis virus in human microglial cells","volume":"16","author":"Zhang","year":"2019","journal-title":"Virol. J."},{"key":"10.1016\/j.fsi.2025.111000_bib42","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2^\u2212\u0394\u0394Ct method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2025.111000_bib43","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1186\/1743-422X-8-354","article-title":"Ectopic expression of microRNA-155 enhances innate antiviral immunity against HBV infection in human hepatoma cells","volume":"8","author":"Su","year":"2011","journal-title":"Virol. J."},{"issue":"10","key":"10.1016\/j.fsi.2025.111000_bib44","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0046082","article-title":"MicroRNA-155 modulates Treg and Th17 cells differentiation and Th17 cell function by targeting SOCS1","volume":"7","author":"Yao","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2025.111000_bib45","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.fsi.2017.07.052","article-title":"Generation of a recombinant viral hemorrhagic septicemia virus (VHSV) expressing olive flounder (Paralichthys olivaceus) interferon-\u03b3 and its effects on type I interferon response and virulence","volume":"68","author":"Kwak","year":"2017","journal-title":"Fish Shellfish Immunol."}],"container-title":["Fish &amp; Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464825008897?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464825008897?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T14:35:37Z","timestamp":1773326137000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464825008897"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":45,"alternative-id":["S1050464825008897"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2025.111000","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Protective efficacy of miR-155 encapsulated using MS2 bacteriophage-based virus-like particle (MS2-VLP) against viral hemorrhagic septicemia virus (VHSV) in olive flounder","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2025.111000","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"111000"}}