{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T14:26:32Z","timestamp":1773930392047,"version":"3.50.1"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021856","name":"Ministero dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["PNRA22_0000059"],"award-info":[{"award-number":["PNRA22_0000059"]}],"id":[{"id":"10.13039\/501100021856","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100024370","name":"Ministero dell'Istruzione dell'Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100024370","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.fsi.2025.111078","type":"journal-article","created":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T16:53:07Z","timestamp":1765903987000},"page":"111078","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["DNA methylation analysis uncovers a fine-tuned regulation of expression of the polymeric Ig receptor gene in the Antarctic teleost Trematomus bernacchii"],"prefix":"10.1016","volume":"169","author":[{"given":"Alessia","family":"Ametrano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salvatore","family":"Fioriniello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ennio","family":"Cocca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Italiani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8273-7123","authenticated-orcid":false,"given":"Maria Rosaria","family":"Coscia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2025.111078_bib1","first-page":"59","volume":"vol. 30","author":"Eastman","year":"1993"},{"key":"10.1016\/j.fsi.2025.111078_bib2","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1007\/s00300-019-02489-1","article-title":"Recent diversification in an ancient lineage of notothenioid fishes (Bovichtus: notothenioidei)","volume":"42","author":"Near","year":"2019","journal-title":"Polar Biol."},{"key":"10.1016\/j.fsi.2025.111078_bib3","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s00300-004-0667-4","article-title":"The nature of the diversity of Antarctic fishes","volume":"28","author":"Eastman","year":"2005","journal-title":"Polar Biol."},{"key":"10.1016\/j.fsi.2025.111078_bib4","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1146\/annurev-animal-081221-064325","article-title":"Adaptations and diversity of Antarctic fishes: a genomic perspective","volume":"15","author":"Daane","year":"2022","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"10.1016\/j.fsi.2025.111078_bib5","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1038\/173848a0","article-title":"Vertebrates without erythrocytes and blood pigment","volume":"173","author":"Ruud","year":"1954","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2025.111078_bib6","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0378-1119(02)00691-1","article-title":"Tracking the evolutionary loss of hemoglobin expression by the white-blooded Antarctic icefishes","volume":"295","author":"di Prisco","year":"2002","journal-title":"Gene"},{"key":"10.1016\/j.fsi.2025.111078_bib7","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1073\/pnas.92.6.1817","article-title":"Genomic remnants of alpha-globin genes in the hemoglobinless antarctic icefishes","volume":"92","author":"Cocca","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.fsi.2025.111078_bib8","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1006\/fsim.1999.0244","article-title":"Analysis of a cDNA sequence encoding the immunoglobulin heavy chain of the Antarctic teleost Trematomus bernacchii","volume":"10","author":"Coscia","year":"2000","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.111078_bib9","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.ympev.2009.09.033","article-title":"Evolution of the Antarctic teleost immunoglobulin heavy chain gene","volume":"55","author":"Coscia","year":"2010","journal-title":"Mol. Phyl. Evol."},{"key":"10.1016\/j.fsi.2025.111078_bib10","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.margen.2015.06.009","article-title":"New insights into evolution of IgT genes coming from Antarctic teleosts","volume":"24","author":"Giacomelli","year":"2015","journal-title":"Mar. Genom."},{"key":"10.1016\/j.fsi.2025.111078_bib11","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.fsi.2021.05.015","article-title":"The evolutionary puzzle solution for the origins of the partial loss of the C\u03c42 exon in notothenioid fishes","volume":"116","author":"Ametrano","year":"2021","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.111078_bib12","doi-asserted-by":"crossref","first-page":"7783","DOI":"10.3390\/ijms23147783","article-title":"Comparative analysis of the pIgR gene from the Antarctic teleost Trematomus bernacchii reveals distinctive features of cold-adapted Notothenioidei","volume":"23","author":"Ametrano","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fsi.2025.111078_bib13","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1038\/321209a0","article-title":"CpG-rich islands and the function of DNA methylation","volume":"321","author":"Bird","year":"1986","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2025.111078_bib14","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1101\/gad.2037511","article-title":"CpG Islands and the regulation of transcription","volume":"25","author":"Deaton","year":"2011","journal-title":"Genes Dev."},{"key":"10.1016\/j.fsi.2025.111078_bib15","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/npp.2012.112","article-title":"DNA methylation and its basic function","volume":"38","author":"Moore","year":"2013","journal-title":"Neuropsychopharmacology"},{"key":"10.1016\/j.fsi.2025.111078_bib16","doi-asserted-by":"crossref","DOI":"10.3389\/fgene.2022.994471","article-title":"Genetic and epigenetic regulation of growth, reproduction, disease resistance and stress responses in aquaculture","volume":"13","author":"Liu","year":"2022","journal-title":"Front. Genet."},{"key":"10.1016\/j.fsi.2025.111078_bib17","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1007\/s11160-024-09887-7","article-title":"The role of DNA methylation in facilitating life history trait diversity in fishes","volume":"34","author":"Kho","year":"2024","journal-title":"Rev. Fish Biol. Fish."},{"key":"10.1016\/j.fsi.2025.111078_bib18","doi-asserted-by":"crossref","first-page":"896","DOI":"10.3390\/biom15060896","article-title":"Identification of DNA methyltransferases (Dnmts) in fish and its potential roles during sex change in blackhead seabream","volume":"15","author":"Guo","year":"2025","journal-title":"Biomolecules"},{"key":"10.1016\/j.fsi.2025.111078_bib19","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.cbpb.2018.01.006","article-title":"Epigenetic in teleost fish: from molecular mechanisms to physiological phenotypes","volume":"224","author":"Best","year":"2018","journal-title":"Comp. Biochem. Physiol. B Biochem. Mol. Biol."},{"key":"10.1016\/j.fsi.2025.111078_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2021.104079","article-title":"Mucosal immunoglobulins of teleost fish: a decade of advances","volume":"121","author":"Salinas","year":"2021","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2025.111078_bib21","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1093\/bioinformatics\/18.11.1427","article-title":"MethPrimer: designing primers for methylation PCRs","volume":"18","author":"Li","year":"2002","journal-title":"Bioinformatics"},{"key":"10.1016\/j.fsi.2025.111078_bib22","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.fsi.2025.111078_bib23","doi-asserted-by":"crossref","first-page":"3412","DOI":"10.1038\/s41467-023-38567-6","article-title":"Genomics of cold adaptations in the Antarctic notothenioid fish radiation","volume":"14","author":"Bista","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.fsi.2025.111078_bib24","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.gene.2006.05.031","article-title":"DNA methylation and body temperature in fishes","volume":"385","author":"Varriale","year":"2006","journal-title":"Gene"},{"key":"10.1016\/j.fsi.2025.111078_bib25","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-10861-6","article-title":"Small ocean temperature increases elicit stage-dependent changes in DNA methylation and gene expression in a fish, the European sea bass","volume":"7","author":"Anastasiadi","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fsi.2025.111078_bib26","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1038\/s41437-020-0346-4","article-title":"Rearing environment affects the genetic architecture and plasticity of DNA methylation in Chinook salmon","volume":"126","author":"Venney","year":"2020","journal-title":"Heredity"},{"key":"10.1016\/j.fsi.2025.111078_bib27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aquaculture.2019.02.045","article-title":"Polymeric immunoglobulin receptor and mucosal IgM responses elicited by immersion and injection vaccination with inactivated Vibrio anguillarum in flounder (Paralichthys olivaceus)","volume":"505","author":"Sheng","year":"2019","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2025.111078_bib28","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.108814","article-title":"Polymeric immunoglobulin receptor (pIgR) in ray-finned fish (Actinopterygii)","volume":"138","author":"Stosik","year":"2023","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.111078_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.109583","article-title":"Possible transport routes of IgM to the gut of teleost fish","volume":"149","author":"Etayo","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2025.111078_bib30","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1038\/nmeth.1828","article-title":"DNA methylome analysis using short bisulfite sequencing data","volume":"9","author":"Krueger","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.fsi.2025.111078_bib31","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1111\/j.1365-2443.2007.01136.x","article-title":"A new class of tissue-specifically methylated regions involving entire CpG islands in the mouse","volume":"12","author":"Suzuki","year":"2007","journal-title":"Genes Cells"},{"key":"10.1016\/j.fsi.2025.111078_bib32","doi-asserted-by":"crossref","first-page":"1969","DOI":"10.1101\/gr.074070.107","article-title":"DNA methylation profile of tissue-dependent and differentially methylated regions (T-DMRs) in mouse promoter regions demonstrating tissue-specific gene expression","volume":"18","author":"Yagi","year":"2008","journal-title":"Genome Res."},{"key":"10.1016\/j.fsi.2025.111078_bib33","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1038\/nrg3000","article-title":"Epigenome-wide association studies for common human diseases","volume":"12","author":"Rakyan","year":"2012","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.fsi.2025.111078_bib34","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1038\/s41588-022-01241-6","article-title":"Evidence that direct inhibition of transcription factor binding is prevailing mode of gene and repeat repression by DNA methylation","volume":"54","author":"Kaluscha","year":"2022","journal-title":"Nat. Genet."},{"key":"10.1016\/j.fsi.2025.111078_bib35","doi-asserted-by":"crossref","first-page":"19359","DOI":"10.1073\/pnas.1921719117","article-title":"DNA methylation enables transposable element-driven genome expansion","volume":"117","author":"Zhou","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.fsi.2025.111078_bib36","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.ympev.2010.12.020","article-title":"Identification of a novel helitron transposon in the genome of antarctic fish","volume":"58","author":"Cocca","year":"2011","journal-title":"Mol. Phyl. Evol."},{"key":"10.1016\/j.fsi.2025.111078_bib37","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1101\/gr.234229.118","article-title":"Switching roles for DNA and histone methylation depend on evolutionary ages of human endogenous retroviruses","volume":"28","author":"Ohtani","year":"2018","journal-title":"Genome Res."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464825009672?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464825009672?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T11:31:12Z","timestamp":1773919872000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464825009672"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":37,"alternative-id":["S1050464825009672"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2025.111078","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"DNA methylation analysis uncovers a fine-tuned regulation of expression of the polymeric Ig receptor gene in the Antarctic teleost Trematomus bernacchii","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2025.111078","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"111078"}}