{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T00:49:11Z","timestamp":1793321351172,"version":"4.5.0"},"reference-count":42,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012601","name":"South China Agricultural University","doi-asserted-by":"publisher","award":["NSYYKY202304"],"award-info":[{"award-number":["NSYYKY202304"]}],"id":[{"id":"10.13039\/501100012601","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013503","name":"Canadian Society for Molecular Biosciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013503","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015956","name":"Special Project for Research and Development in Key areas of Guangdong Province","doi-asserted-by":"publisher","award":["2021B0202040002"],"award-info":[{"award-number":["2021B0202040002"]}],"id":[{"id":"10.13039\/501100015956","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2026,5]]},"DOI":"10.1016\/j.fsi.2026.111226","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T07:49:50Z","timestamp":1771573790000},"page":"111226","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Oral administration of IFN-\u03b3rel elicits antiviral immunity and enhances protective efficacy against LMBV in largemouth bass (Micropterus salmoides)"],"prefix":"10.1016","volume":"172","author":[{"given":"Zemiao","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sen","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juncheng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiemin","family":"Xian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youhua","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiwei","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2026.111226_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2019.734901","article-title":"Aquaculture industry development, annual price analysis and out-of-season spawning in largemouth bass Micropterus salmoides","volume":"519","author":"Hussein","year":"2020","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2026.111226_bib2","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.repbre.2024.01.004","article-title":"Current status and application of largemouth bass (Micropterus salmoides) germplasm resources","volume":"4","author":"Yu","year":"2024","journal-title":"Reprod. Breeding"},{"key":"10.1016\/j.fsi.2026.111226_bib3","series-title":"Fisheries Bureau, Fishery Statistical Yearbook","year":"2025"},{"key":"10.1016\/j.fsi.2026.111226_bib4","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.virol.2017.06.007","article-title":"Ranaviruses and other members of the family Iridoviridae: their place in the virosphere","volume":"511","author":"Chinchar","year":"2017","journal-title":"Virology"},{"key":"10.1016\/j.fsi.2026.111226_bib5","doi-asserted-by":"crossref","first-page":"107","DOI":"10.3354\/dao037107","article-title":"Molecular characterization of a ranavirus isolated from largemouth bass Micropterus salmoides","volume":"37","author":"Mao","year":"1999","journal-title":"Dis. Aquat. Organ."},{"key":"10.1016\/j.fsi.2026.111226_bib6","article-title":"Largemouth bass ranavirus: current status and research progression","volume":"32","author":"Zhao","year":"2023","journal-title":"Aquacult. Rep."},{"key":"10.1016\/j.fsi.2026.111226_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2023.740527","article-title":"Artificial infection pathway of largemouth bass LMBV and identification of resistant and susceptible individuals","volume":"582","author":"Zhang","year":"2024","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2026.111226_bib8","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1046\/j.1365-2761.2002.00418.x","article-title":"Experimental oral transmission of largemouth bass virus","volume":"25","author":"Woodland","year":"2002","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2026.111226_bib9","doi-asserted-by":"crossref","first-page":"143","DOI":"10.3354\/dao039143","article-title":"Preliminary description of lesions in juvenile largemouth bass injected with largemouth bass virus","volume":"39","author":"Zilberg","year":"2000","journal-title":"Dis. Aquat. Organ."},{"key":"10.1016\/j.fsi.2026.111226_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.108753","article-title":"Transcriptome analysis reveals the host immune response upon LMBV infection in largemouth bass (Micropterus salmoides)","volume":"137","author":"Xu","year":"2023","journal-title":"FIsh Shellfish immun."},{"key":"10.1016\/j.fsi.2026.111226_bib11","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1146\/annurev-virology-100114-055249","article-title":"No love lost between viruses and interferons","volume":"2","author":"Fensterl","year":"2015","journal-title":"Annu. Rev. Virol."},{"key":"10.1016\/j.fsi.2026.111226_bib12","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1038\/nri2314","article-title":"Interferon-inducible antiviral effectors","volume":"8","author":"Sadler","year":"2008","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.fsi.2026.111226_bib13","article-title":"Identification and establishment of type IV interferon and the characterization of interferon-\u03c5 including its class II cytokine receptors IFN-\u03c5R1 and IL-10R2","volume":"13","author":"Chen","year":"2022","journal-title":"Nat. Commun."},{"key":"10.1016\/j.fsi.2026.111226_bib14","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1111\/raq.12349","article-title":"Fish type I and type II interferons: composition, receptor usage, production and function","volume":"12","author":"Gan","year":"2020","journal-title":"Rev. Aquacult."},{"key":"10.1016\/j.fsi.2026.111226_bib15","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1016\/j.dci.2011.07.001","article-title":"Teleost fish interferons and their role in immunity","volume":"35","author":"Zou","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2026.111226_bib16","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.dci.2019.03.016","article-title":"Receptor complex and signalling pathway of the two type II IFNs, IFN-\u03b3 and IFN-\u03b3rel in mandarin fish or the so-called Chinese perch Siniperca chuatsi","volume":"97","author":"Li","year":"2019","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2026.111226_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.109292","article-title":"Type II interferons (IFN-\u03b3 and IFN-\u03b3rel) activate downstream genes through various potential receptor combinations to exert antiviral functions in spotted sea bass (Lateolabrax maculatus)","volume":"145","author":"Yan","year":"2024","journal-title":"FIsh Shellfish immun."},{"key":"10.1016\/j.fsi.2026.111226_bib18","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1111\/febs.12666","article-title":"Peculiar monomeric interferon gammas, IFN\u03b3rel 1 and IFN\u03b3rel 2, in ginbuna crucian carp","volume":"281","author":"Shibasaki","year":"2014","journal-title":"FEBS J."},{"key":"10.1016\/j.fsi.2026.111226_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2020.736314","article-title":"Antiviral activities of sea perch type I and type II IFNs against RGNNV and their different roles in antigen presentation","volume":"534","author":"Lu","year":"2021","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2026.111226_bib20","doi-asserted-by":"crossref","first-page":"3302","DOI":"10.1021\/acs.biomac.7b00959","article-title":"Development of a drug delivery system based on chitosan nanoparticles for oral administration of interferon-alpha","volume":"18","author":"Canepa","year":"2017","journal-title":"Biomacromolecules"},{"key":"10.1016\/j.fsi.2026.111226_bib21","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.696781","article-title":"Lactococcus lactis expressing type I interferon from Atlantic salmon enhances the innate antiviral immune response In Vivo and In Vitro","volume":"12","author":"Munoz","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2026.111226_bib22","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2021.696803","article-title":"Oral administration of Lactococcus lactis producing interferon type II, enhances the immune response against bacterial pathogens in rainbow trout","volume":"12","author":"Santibanez","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2026.111226_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2024.132872","article-title":"Preparation and evaluation of microencapsulated delivery system of recombinant interferon alpha protein from rainbow trout","volume":"273","author":"Ouyang","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.fsi.2026.111226_bib24","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.aquaculture.2013.01.017","article-title":"Immunoprotection of grass carp (Ctenopharyngodon idella) with recombinant interferon (rCiIFN) against GCHV infection","volume":"388","author":"Li","year":"2013","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2026.111226_bib25","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1093\/bioinformatics\/btm066","article-title":"NucPred--predicting nuclear localization of proteins","volume":"23","author":"Brameier","year":"2007","journal-title":"Bioinformatics"},{"key":"10.1016\/j.fsi.2026.111226_bib26","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/1471-2105-4-29","article-title":"MatGAT: an application that generates similarity\/identity matrices using protein or DNA sequences","volume":"4","author":"Campanella","year":"2003","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.fsi.2026.111226_bib27","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1128\/jb.99.1.70-77.1969","article-title":"Conditions for competence in the Bacillus licheniformis transformation system","volume":"99","author":"Spizizen","year":"1969","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.fsi.2026.111226_bib28","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/s12964-020-0514-4","article-title":"Immune response in piglets orally immunized with recombinant Bacillus subtilis expressing the capsid protein of porcine circovirus type 2","volume":"18","author":"Zhang","year":"2020","journal-title":"Cell Commun. Signal."},{"key":"10.1016\/j.fsi.2026.111226_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.108627","article-title":"Surface display of major capsid protein on Bacillus subtilis spores against largemouth bass virus (LMBV) for oral administration","volume":"135","author":"Wang","year":"2023","journal-title":"FIsh Shellfish immun."},{"key":"10.1016\/j.fsi.2026.111226_bib30","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1016\/S1473-3099(14)70920-X","article-title":"Ribavirin and interferon alfa-2a for severe Middle East respiratory syndrome coronavirus infection: a retrospective cohort study","volume":"14","author":"Omrani","year":"2014","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.fsi.2026.111226_bib31","article-title":"Interferon alpha-2b spray shortened viral shedding time of SARS-CoV-2 Omicron variant: an open prospective cohort study","volume":"13","author":"Xu","year":"2022","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2026.111226_bib32","article-title":"IFN-gamma enhances protective efficacy against Nocardia seriolae infection in largemouth bass (Micropterus salmoides)","volume":"15","author":"Yu","year":"2024","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2026.111226_bib33","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.vetimm.2009.09.007","article-title":"Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gammarel) in grass carp Ctenopharyngodon idella","volume":"134","author":"Chen","year":"2010","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.fsi.2026.111226_bib34","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1111\/raq.12503","article-title":"Multifaceted applications of probiotic bacillus species in aquaculture with special reference to Bacillus subtilis","volume":"13","author":"Nayak","year":"2021","journal-title":"Rev. Aquacult."},{"key":"10.1016\/j.fsi.2026.111226_bib35","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1016\/j.fsi.2018.10.058","article-title":"Characterization of Bacillus subtilis from gastrointestinal tract of hybrid Hulong grouper (Epinephelus fuscoguttatus x E - lanceolatus) and its effects as probiotic additives","volume":"84","author":"Zhou","year":"2019","journal-title":"FIsh Shellfish immun."},{"key":"10.1016\/j.fsi.2026.111226_bib36","article-title":"Secretory production of functional grouper type I interferon from Epinephelus septemfasciatus in Escherichia coli and Bacillus subtilis","volume":"21","author":"Lin","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fsi.2026.111226_bib37","doi-asserted-by":"crossref","DOI":"10.1128\/AEM.02938-19","article-title":"Engineering Bacillus subtilis as a Versatile and stable platform for production of nanobodies","volume":"86","author":"Yang","year":"2020","journal-title":"Appl. Environ. Microb."},{"key":"10.1016\/j.fsi.2026.111226_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.antiviral.2023.105644","article-title":"Recombinant porcine interferon cocktail delays the onset and lessens the severity of African swine fever","volume":"215","author":"Jiao","year":"2023","journal-title":"Antivir. Res."},{"key":"10.1016\/j.fsi.2026.111226_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.antiviral.2020.104791","article-title":"Type 1 interferons as a potential treatment against COVID-19","volume":"178","author":"Sallard","year":"2020","journal-title":"Antivir. Res."},{"key":"10.1016\/j.fsi.2026.111226_bib40","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.antiviral.2016.09.003","article-title":"Single dose of an adenovirus vectored mouse interferon-alpha protects mice from lethal EV71 challenge","volume":"134","author":"Sun","year":"2016","journal-title":"Antivir. Res."},{"key":"10.1016\/j.fsi.2026.111226_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.109875","article-title":"Oral administration of recombinant Lactococcus lactis expressing largemouth bass (Micropterus salmoides) IFNa3 protein enhances immune response against largemouth bass virus (LMBV) infection","volume":"154","author":"Hua","year":"2024","journal-title":"FIsh Shellfish immun."},{"key":"10.1016\/j.fsi.2026.111226_bib42","doi-asserted-by":"crossref","first-page":"3625","DOI":"10.1172\/JCI126363","article-title":"IFN-I response timing relative to virus replication determines MERS coronavirus infection outcomes","volume":"129","author":"Channappanavar","year":"2019","journal-title":"J. Clin. Investig."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826001300?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826001300?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T03:38:19Z","timestamp":1777606699000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464826001300"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":42,"alternative-id":["S1050464826001300"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111226","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Oral administration of IFN-\u03b3rel elicits antiviral immunity and enhances protective efficacy against LMBV in largemouth bass (Micropterus salmoides)","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111226","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"111226"}}