{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T21:15:54Z","timestamp":1783199754925,"version":"3.54.6"},"reference-count":44,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Shandong Province Natural Science Foundation","doi-asserted-by":"publisher","award":["ZR2023QC075"],"award-info":[{"award-number":["ZR2023QC075"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.fsi.2026.111493","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T05:44:07Z","timestamp":1780897447000},"page":"111493","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Identification and functional characterization of Ism1 as a new member of antimicrobial protein"],"prefix":"10.1016","volume":"176","author":[{"given":"Yang","family":"Zhou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songtao","family":"Hao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yetong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feixiang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maoheng","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6395-160X","authenticated-orcid":false,"given":"Shousheng","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2026.111493_bib1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0925-4773(02)00123-5","article-title":"Isthmin is a novel secreted protein expressed as part of the Fgf-8 synexpression group in the Xenopus midbrain\u2013hindbrain organizer","volume":"116","author":"Pera","year":"2002","journal-title":"Mech. Dev."},{"key":"10.1016\/j.fsi.2026.111493_bib2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.gene.2004.03.008","article-title":"TAIL1: an isthmin-like gene, containing type 1 thrombospondin-repeat and AMOP domain, mapped to ARVD1 critical region","volume":"335","author":"Rossi","year":"2004","journal-title":"Gene"},{"key":"10.1016\/j.fsi.2026.111493_bib3","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.4161\/cc.28494","article-title":"Distinct spatiotemporal expression of ISM1 during mouse and chick development","volume":"13","author":"Os\u00f3rio","year":"2014","journal-title":"Cell Cycle"},{"key":"10.1016\/j.fsi.2026.111493_bib4","doi-asserted-by":"crossref","first-page":"17","DOI":"10.3390\/cells12010017","article-title":"Isthmin-A multifaceted protein family","volume":"12","author":"Shakhawat","year":"2022","journal-title":"Cells"},{"key":"10.1016\/j.fsi.2026.111493_bib5","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1007\/s12079-023-00732-3","article-title":"Advances in research of biological functions of Isthmin-1","volume":"17","author":"Menghuan","year":"2023","journal-title":"J. Cell Commun. Signal."},{"key":"10.1016\/j.fsi.2026.111493_bib6","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1007\/s00441-020-03318-2","article-title":"Isthmin1, a secreted signaling protein, acts downstream of diverse embryonic patterning centers in development","volume":"383","author":"Kesavan","year":"2021","journal-title":"Cell Tissue Res."},{"key":"10.1016\/j.fsi.2026.111493_bib7","doi-asserted-by":"crossref","first-page":"2378","DOI":"10.1038\/s41467-023-37992-x","article-title":"Isthmin-1 (Ism1) modulates renal branching morphogenesis and mesenchyme condensation during early kidney development","volume":"14","author":"Gao","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.fsi.2026.111493_bib8","doi-asserted-by":"crossref","DOI":"10.1002\/bies.202300189","article-title":"Isthmin-1: a critical regulator of branching morphogenesis and metanephric mesenchyme condensation during early kidney development","volume":"46","author":"Gao","year":"2024","journal-title":"Bioessays"},{"key":"10.1016\/j.fsi.2026.111493_bib9","doi-asserted-by":"crossref","first-page":"2388","DOI":"10.1083\/jcb.201801081","article-title":"ISM1 regulates NODAL signaling and asymmetric organ morphogenesis during development","volume":"218","author":"Os\u00f3rio","year":"2019","journal-title":"JCB (J. Cell Biol.)"},{"key":"10.1016\/j.fsi.2026.111493_bib10","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1534\/genetics.117.300535","article-title":"Identification of Isthmin 1 as a novel clefting and craniofacial patterning gene in humans","volume":"208","author":"Lansdon","year":"2018","journal-title":"Genetics"},{"key":"10.1016\/j.fsi.2026.111493_bib11","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0196872","article-title":"Isthmin 1 (ism1) is required for normal hematopoiesis in developing zebrafish","volume":"13","author":"Berrun","year":"2018","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2026.111493_bib12","volume":"119","author":"Lam","year":"2022"},{"key":"10.1016\/j.fsi.2026.111493_bib13","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1186\/s10020-022-00500-w","article-title":"ISM1 suppresses LPS-induced acute lung injury and post-injury lung fibrosis in mice","volume":"28","author":"Nguyen","year":"2022","journal-title":"Mol. Med."},{"key":"10.1016\/j.fsi.2026.111493_bib14","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2021.104210","article-title":"Zebrafish Ism1 is a novel antiviral factor that positively regulates antiviral immune responses","volume":"125","author":"Li","year":"2021","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2026.111493_bib15","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.3390\/microorganisms13061280","article-title":"Isthmin1 upregulation in the intestinal microenvironment during Salmonella Typhimurium infection: identification and characterization of Isthmin1-Producing cells","volume":"13","author":"Jaimes-Ortega","year":"2025","journal-title":"Microorganisms"},{"key":"10.1016\/j.fsi.2026.111493_bib16","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2022\/2909487","article-title":"Isthmin 1 is expressed by progenitor-like cells in the lung: phenotypical analysis of isthmin 1+ hematopoietic stem-like cells in homeostasis and during infection","author":"Rivera-Torruco","year":"2022","journal-title":"J. Immunol. Res."},{"key":"10.1016\/j.fsi.2026.111493_bib17","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1089\/jir.2013.0137","article-title":"Isthmin 1 is a secreted protein expressed in skin, mucosal tissues, and NK, NKT, and Th17 cells","volume":"34","author":"Valle-Rios","year":"2014","journal-title":"J. Interferon Cytokine Res."},{"key":"10.1016\/j.fsi.2026.111493_bib18","doi-asserted-by":"crossref","first-page":"41734","DOI":"10.1074\/jbc.M406267200","article-title":"Identification of novel beta1 integrin binding sites in the type 1 and type 2 repeats of thrombospondin-1","volume":"279","author":"Calzada","year":"2004","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fsi.2026.111493_bib19","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1038\/emboj.2008.288","article-title":"Structure of the F-spondin domain of mindin, an integrin ligand and pattern recognition molecule","volume":"28","author":"Li","year":"2009","journal-title":"EMBO J."},{"key":"10.1016\/j.fsi.2026.111493_bib20","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/S0968-0004(01)02049-7","article-title":"A protein module alternatively spliced in cancer cells","volume":"27","author":"Ciccarelli","year":"2002","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.fsi.2026.111493_bib21","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1038\/nprot.2007.514","article-title":"High-resolution in situ hybridization to whole-mount zebrafish embryos","volume":"3","author":"Thisse","year":"2007","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.fsi.2026.111493_bib22","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/s42003-021-01777-z","article-title":"ELAVL1a is an immunocompetent protein that protects zebrafish embryos from bacterial infection","volume":"4","author":"Ni","year":"2021","journal-title":"Commun. Biol."},{"key":"10.1016\/j.fsi.2026.111493_bib23","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2026.111493_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.108671","article-title":"Identification and functional characterization of zebrafish ELAVL1b as a new member of antimicrobial protein","volume":"135","author":"Zhou","year":"2023","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111493_bib25","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.109451","article-title":"Zebrafish ANGPT4, member of fibrinogen-related proteins, is an LTA-, LPS- and PGN-binding protein with a bacteriolytic activity","volume":"147","author":"Zhou","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111493_bib26","doi-asserted-by":"crossref","first-page":"15787","DOI":"10.1021\/acs.jafc.2c06297","article-title":"Folic acid protects against hyperuricemia in C57BL\/6J mice via ameliorating gut-kidney axis dysfunction","volume":"70","author":"Wang","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.fsi.2026.111493_bib27","doi-asserted-by":"crossref","first-page":"19531","DOI":"10.1021\/acs.jafc.3c05529","article-title":"Astaxanthin protects against alcoholic liver injury via regulating mitochondrial redox balance and calcium homeostasis","volume":"71","author":"Wang","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"10.1016\/j.fsi.2026.111493_bib28","doi-asserted-by":"crossref","first-page":"12983","DOI":"10.1096\/fj.201901290R","article-title":"Identification of ATP synthase \u03b1 subunit as a new maternal factor capable of protecting zebrafish embryos from bacterial infection","volume":"33","author":"Ni","year":"2019","journal-title":"FASEB J."},{"key":"10.1016\/j.fsi.2026.111493_bib29","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.1021\/pr3009226","article-title":"Novel blood collection method allows plasma proteome analysis from single zebrafish","volume":"12","author":"Babaei","year":"2013","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.fsi.2026.111493_bib30","doi-asserted-by":"crossref","DOI":"10.1101\/pdb.prot100271","article-title":"Sampling and preparation of mouse and rat serum","author":"Greenfield","year":"2017","journal-title":"Cold Spring Harb. Protoc."},{"key":"10.1016\/j.fsi.2026.111493_bib31","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1016\/j.molimm.2009.02.029","article-title":"A novel C-type lectin with two CRD domains from Chinese shrimp Fenneropenaeus chinensis functions as a pattern recognition protein","volume":"46","author":"Zhang","year":"2009","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2026.111493_bib32","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1007\/s10522-019-09799-1","article-title":"Administration of rGDF11 retards the aging process in male mice via action of anti-oxidant system","volume":"20","author":"Zhou","year":"2019","journal-title":"Biogerontology"},{"key":"10.1016\/j.fsi.2026.111493_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.cyto.2023.156423","article-title":"Isthmin: a multifunctional secretion protein","volume":"173","author":"Liang","year":"2024","journal-title":"Cytokine"},{"key":"10.1016\/j.fsi.2026.111493_bib34","doi-asserted-by":"crossref","first-page":"3464","DOI":"10.1111\/febs.14622","article-title":"The ribosome biogenesis protein Esf1 is essential for pharyngeal cartilage formation in zebrafish","volume":"285","author":"Chen","year":"2018","journal-title":"FEBS J."},{"key":"10.1016\/j.fsi.2026.111493_bib35","series-title":"The Kinesin Superfamily Handbook: Transporter, Creator, Destroyer","first-page":"41","article-title":"The Kinesin-3 family: long-distance transporters","author":"Siddiqui","year":"2020"},{"key":"10.1016\/j.fsi.2026.111493_bib36","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1038\/s42003-024-06018-7","article-title":"Microtubule polarity determines the lineage of embryonic neural precursor in zebrafish spinal cord","volume":"7","author":"Richard","year":"2024","journal-title":"Commun. Biol."},{"key":"10.1016\/j.fsi.2026.111493_bib37","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1016\/j.fsi.2022.10.065","article-title":"Bactericidal permeability-increasing protein\/LPS-binding protein (BPI\/LBP) enhances resistance of golden pompano Trachinotus ovatus against bacterial infection","volume":"131","author":"Wu","year":"2022","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111493_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.109989","article-title":"A novel LPS binding\/bactericidal permeability-increasing protein (LBP\/BPI) from the scallop Argopecten purpuratus plays an essential role in host resistance to vibrio infection","volume":"154","author":"Jorquera","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111493_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.109791","article-title":"Ribosomal protein L17 functions as an antimicrobial protein in amphioxus","volume":"152","author":"Zhou","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111493_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.110063","article-title":"Identification of a novel antimicrobial peptide from amphioxus ribosomal protein L27","volume":"157","author":"Chen","year":"2025","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111493_bib41","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.fsi.2018.08.012","article-title":"The hepatic lectin of zebrafish binds a wide range of bacteria and participates in immune defense","volume":"82","author":"Yang","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111493_bib42","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1016\/j.fsi.2019.10.068","article-title":"A novel hepatic lectin of zebrafish Danio rerio is involved in innate immune defense","volume":"98","author":"Yang","year":"2020","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111493_bib43","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.fsi.2016.03.016","article-title":"Identification and functional characterization of fish-egg lectin in zebrafish","volume":"52","author":"Wang","year":"2016","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111493_bib44","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2021.104332","article-title":"Cross-species opsonic activity of zebrafish fish-egg lectin on mouse macrophages","volume":"129","author":"Qiao","year":"2022","journal-title":"Dev. Comp. Immunol."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826003979?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826003979?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T20:34:08Z","timestamp":1783197248000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464826003979"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":44,"alternative-id":["S1050464826003979"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111493","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Identification and functional characterization of Ism1 as a new member of antimicrobial protein","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111493","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"111493"}}