{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T20:15:42Z","timestamp":1793909742416,"version":"4.8.0"},"reference-count":63,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/100020760","name":"Sanya Yazhou Bay Science and Technology City","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100020760","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.fsi.2026.111504","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T06:33:32Z","timestamp":1781159612000},"page":"111504","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["Oral immunization of Nile tilapia with Chlorella-bioencapsulated multi-epitope fusion antigen effectively protects against Streptococcus agalactiae infection"],"prefix":"10.1016","volume":"176","author":[{"given":"Xiaodong","family":"Deng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zichun","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Mu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dianlong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juncai","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxuan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanjie","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonghua","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4228-6516","authenticated-orcid":false,"given":"Xiaowen","family":"Fei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2026.111504_bib1","first-page":"914","article-title":"Streptococcus agalactiae infection in Nile tilapia (Oreochromis niloticus): a review","volume":"13","author":"Abdallah","year":"2024","journal-title":"Biology (Basel)"},{"key":"10.1016\/j.fsi.2026.111504_bib2","doi-asserted-by":"crossref","first-page":"163","DOI":"10.3354\/dao03084","article-title":"Development of Streptococcus agalactiae vaccines for tilapia","volume":"122","author":"Liu","year":"2016","journal-title":"Dis. Aquat. Org."},{"key":"10.1016\/j.fsi.2026.111504_bib3","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1002\/aah.10132","article-title":"Prevention and control of streptococcosis in tilapia culture: a systematic review","volume":"33","author":"Maulu","year":"2021","journal-title":"J. Aquat. Anim. Health"},{"key":"10.1016\/j.fsi.2026.111504_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.109572","article-title":"Recombinant lactococcal-based oral vaccine for protection against Streptococcus agalactiae infections in tilapia (Oreochromis niloticus)","volume":"149","author":"Wong","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111504_bib5","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2020.622377","article-title":"Systemic and mucosal B and T cell responses upon mucosal vaccination of teleost fish","volume":"11","author":"Mu\u00f1oz-Atienza","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2026.111504_bib6","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1016\/j.fsi.2010.09.001","article-title":"Teleost intestinal immunology","volume":"31","author":"Rombout","year":"2011","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111504_bib7","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/j.fsi.2014.08.020","article-title":"Adaptive immune responses at mucosal surfaces of teleost fish","volume":"40","author":"Rombout","year":"2014","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111504_bib8","first-page":"525","article-title":"The mucosal immune system of teleost fish","volume":"4","author":"Salinas","year":"2015","journal-title":"Biology (Basel)"},{"key":"10.1016\/j.fsi.2026.111504_bib9","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2020.567941","article-title":"Immunoglobulins, mucosal immunity and vaccination in teleost fish","volume":"11","author":"Yu","year":"2020","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2026.111504_bib10","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.fsi.2014.02.017","article-title":"Safety and immunogenicity of an oral DNA vaccine encoding Sip of Streptococcus agalactiae from Nile tilapia Oreochromis niloticus delivered by live attenuated Salmonella typhimurium","volume":"38","author":"Huang","year":"2014","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111504_bib11","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1016\/j.fsi.2018.12.060","article-title":"Oral vaccination of tilapia against Streptococcus agalactiae using Bacillus subtilis spores expressing Sip","volume":"86","author":"Yao","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111504_bib12","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.dci.2017.07.024","article-title":"Effectivity of oral recombinant DNA vaccine against Streptococcus agalactiae in Nile tilapia","volume":"77","author":"Zhu","year":"2017","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2026.111504_bib13","doi-asserted-by":"crossref","first-page":"8231","DOI":"10.1111\/1750-3841.17529","article-title":"Chlorella vulgaris as a food substitute: applications and benefits in the food industry","volume":"89","author":"Wang","year":"2024","journal-title":"J. Food Sci."},{"key":"10.1016\/j.fsi.2026.111504_bib14","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.fsi.2018.04.011","article-title":"Influences of Chlorella vulgaris dietary supplementation on growth performance, hematology, immune response and disease resistance in Oreochromis niloticus exposed to sub-lethal concentrations of penoxsulam herbicide","volume":"77","author":"Galal","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111504_bib15","doi-asserted-by":"crossref","DOI":"10.3389\/fbioe.2020.590431","article-title":"Evaluation of microalgae as immunostimulants and recombinant vaccines for diseases prevention and control in aquaculture","volume":"8","author":"Ma","year":"2020","journal-title":"Front. Bioeng. Biotechnol."},{"key":"10.1016\/j.fsi.2026.111504_bib16","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/j.fsi.2014.09.035","article-title":"Modulatory role of dietary Chlorella vulgaris powder against arsenic-induced immunotoxicity and oxidative stress in Nile tilapia (Oreochromis niloticus)","volume":"41","author":"Zahran","year":"2014","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111504_bib17","doi-asserted-by":"crossref","first-page":"223","DOI":"10.3390\/synbio2020013","article-title":"Recombinant protein expression and its biotechnological applications in Chlorella spp","volume":"2","author":"Chen","year":"2024","journal-title":"SynBio"},{"key":"10.1016\/j.fsi.2026.111504_bib18","doi-asserted-by":"crossref","first-page":"60","DOI":"10.3389\/fmicb.2014.00060","article-title":"Algae-based oral recombinant vaccines","volume":"5","author":"Specht","year":"2014","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.fsi.2026.111504_bib19","doi-asserted-by":"crossref","first-page":"2694","DOI":"10.1016\/j.tibtech.2025.04.001","article-title":"Microalgae-based vaccines for aquaculture","volume":"43","author":"Chen","year":"2025","journal-title":"Trends Biotechnol."},{"key":"10.1016\/j.fsi.2026.111504_bib20","article-title":"Utilization of recombinant Chlorella expressing dsRNA for protection against grouper nervous necrosis virus","volume":"38","author":"Wei","year":"2024","journal-title":"Aquac. Rep."},{"key":"10.1016\/j.fsi.2026.111504_bib21","article-title":"Immunoprotection of grouper by combining recombinant Chlorella sorokiniana carrying the NNV RdRp shRNA\/dsRNA with Jingfang granules, epigallocatechin gallate, and ribavirin","volume":"48","author":"Fei","year":"2026","journal-title":"Aquac. Rep."},{"key":"10.1016\/j.fsi.2026.111504_bib22","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1007\/s12033-023-00763-6","article-title":"Trypanosoma cruzi Tc24 antigen expressed and orally delivered by Schizochytrium sp. microalga is immunogenic in mice","volume":"66","author":"Ramos-Vega","year":"2024","journal-title":"Mol. Biotechnol."},{"key":"10.1016\/j.fsi.2026.111504_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2023.106488","article-title":"Protective efficacy of the oral vaccine Tc24:Co1 produced in Schizochytrium sp. against Trypanosoma cruzi infection in a mouse model","volume":"186","author":"Ramos-Vega","year":"2024","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.fsi.2026.111504_bib24","doi-asserted-by":"crossref","first-page":"555","DOI":"10.3390\/md22120555","article-title":"Transgenic Schizochytrium as a promising oral vaccine carrier: potential application in the aquaculture industry","volume":"22","author":"Ma","year":"2024","journal-title":"Mar. Drugs"},{"key":"10.1016\/j.fsi.2026.111504_bib25","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.fsi.2019.01.038","article-title":"An evaluation of microalgae as a recombinant protein oral delivery platform for fish using green fluorescent protein (GFP)","volume":"87","author":"Kwon","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111504_bib26","doi-asserted-by":"crossref","first-page":"5610","DOI":"10.1128\/IAI.68.10.5610-5618.2000","article-title":"Identification of group B streptococcal Sip protein, which elicits cross-protective immunity","volume":"68","author":"Brodeur","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fsi.2026.111504_bib27","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1128\/CMR.18.1.102-127.2005","article-title":"Surface proteins of Streptococcus agalactiae and related proteins in other bacterial pathogens","volume":"18","author":"Lindahl","year":"2005","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.fsi.2026.111504_bib28","doi-asserted-by":"crossref","first-page":"18391","DOI":"10.1073\/pnas.0504954102","article-title":"Structure of the streptococcal cell wall C5a peptidase","volume":"102","author":"Brown","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.fsi.2026.111504_bib29","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1002\/prot.26625","article-title":"The 1.7 \u00c5 crystal structure of the C5a peptidase from Streptococcus agalactiae (ScpB) reveals an active site competent for catalysis","volume":"92","author":"Cullen","year":"2024","journal-title":"Proteins"},{"key":"10.1016\/j.fsi.2026.111504_bib30","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1128\/IAI.73.3.1671-1683.2005","article-title":"Characterization of a novel leucine-rich repeat protein antigen from group B streptococci that elicits protective immunity","volume":"73","author":"Seepersaud","year":"2005","journal-title":"Infect. Immun."},{"key":"10.1016\/j.fsi.2026.111504_bib31","article-title":"A review on the development of bacterial multi-epitope recombinant protein vaccines via reverse vaccinology","volume":"282","author":"Li","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.fsi.2026.111504_bib32","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1039\/C5SC03892H","article-title":"Peptide-based synthetic vaccines","volume":"7","author":"Skwarczynski","year":"2016","journal-title":"Chem. Sci."},{"key":"10.1016\/j.fsi.2026.111504_bib33","doi-asserted-by":"crossref","first-page":"392","DOI":"10.3390\/vaccines12040392","article-title":"Immunoinformatics design and in vivo immunogenicity evaluation of a conserved CTL multi-epitope vaccine targeting HPV16 E5, E6, and E7 proteins","volume":"12","author":"Guo","year":"2024","journal-title":"Vaccines"},{"key":"10.1016\/j.fsi.2026.111504_bib34","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.3390\/ph17111498","article-title":"Design, development and immunogenicity study of a multi-epitope vaccine prototype against SARS-CoV-2","volume":"17","author":"Atanasova","year":"2024","journal-title":"Pharmaceuticals"},{"key":"10.1016\/j.fsi.2026.111504_bib35","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2025.1442101","article-title":"Development of a multi-epitope vaccine candidate to combat SARS-CoV-2 and dengue virus co-infection through an immunoinformatic approach","volume":"16","author":"Mandal","year":"2025","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2026.111504_bib36","doi-asserted-by":"crossref","DOI":"10.1016\/j.fawpar.2025.e00269","article-title":"Design and immunological evaluation of a multi-epitope vaccine candidate against Toxoplasma gondii incorporating MIC13, GRA1, and SAG1 antigens in BALB\/c mice","volume":"40","author":"Hosseininejad","year":"2025","journal-title":"Food Waterborne Parasitol"},{"key":"10.1016\/j.fsi.2026.111504_bib37","doi-asserted-by":"crossref","first-page":"31","DOI":"10.3390\/vaccines14010031","article-title":"Development and immunogenicity assessment of a multi-epitope antigen against Zika virus: an in silico and in vivo approach","volume":"14","author":"Leal","year":"2026","journal-title":"Vaccines"},{"key":"10.1016\/j.fsi.2026.111504_bib38","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s00438-026-02376-5","article-title":"Immunoinformatics-guided design of a multi-epitope vaccine targeting the envelopment polyprotein of oropouche virus","volume":"301","author":"Naveed","year":"2026","journal-title":"Mol. Genet. Genom."},{"key":"10.1016\/j.fsi.2026.111504_bib39","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1038\/s41541-026-01409-9","article-title":"A novel multi-epitope vaccine induces protective and therapeutic immunity against Helicobacter pylori","volume":"11","author":"Moeini","year":"2026","journal-title":"npj Vaccines"},{"key":"10.1016\/j.fsi.2026.111504_bib40","series-title":"The Chlamydomonas Source Book: a Comprehensive Guide to Biology and Laboratory Use","author":"Harris","year":"1989"},{"key":"10.1016\/j.fsi.2026.111504_bib41","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1186\/1471-2091-14-38","article-title":"Effect of the expression and knockdown of citrate synthase gene on carbon flux during triacylglycerol biosynthesis by green algae Chlamydomonas reinhardtii","volume":"14","author":"Deng","year":"2013","journal-title":"BMC Biochem."},{"key":"10.1016\/j.fsi.2026.111504_bib42","doi-asserted-by":"crossref","first-page":"101","DOI":"10.14202\/vetworld.2017.101-111","article-title":"Molecular identification and histopathological study of natural Streptococcus agalactiae infection in hybrid tilapia (Oreochromis niloticus)","volume":"10","author":"Laith","year":"2017","journal-title":"Vet. World"},{"key":"10.1016\/j.fsi.2026.111504_bib43","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1128\/jvi.55.3.836-839.1985","article-title":"Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide","volume":"55","author":"Emini","year":"1985","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2026.111504_bib44","doi-asserted-by":"crossref","first-page":"W24","DOI":"10.1093\/nar\/gkx346","article-title":"BepiPred-2.0: improving sequence-based B-cell epitope prediction using conformational epitopes","volume":"45","author":"Jespersen","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.fsi.2026.111504_bib45","doi-asserted-by":"crossref","first-page":"D339","DOI":"10.1093\/nar\/gky1006","article-title":"The immune epitope database (IEDB): 2018 update","volume":"47","author":"Vita","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.fsi.2026.111504_bib46","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2026.111504_bib47","article-title":"Oral immunization of grouper with recombinant Chlamydomonas to prevent viral nervous necrosis and the effect of which on intestinal microbiota","volume":"41","author":"Deng","year":"2025","journal-title":"Aquac. Rep."},{"key":"10.1016\/j.fsi.2026.111504_bib48","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1007\/s13205-021-02750-7","article-title":"Efficient Agrobacterium tumefaciens-mediated stable genetic transformation of green microalgae, Chlorella sorokiniana","volume":"11","author":"Sharma","year":"2021","journal-title":"3 Biotech"},{"key":"10.1016\/j.fsi.2026.111504_bib49","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/978-1-4939-9740-4_20","article-title":"Electroporation procedures for genetic modification of green algae (Chlorella spp.)","volume":"2050","author":"Ji","year":"2020","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.fsi.2026.111504_bib50","doi-asserted-by":"crossref","DOI":"10.1101\/pdb.prot5439","article-title":"Purification of RNA using TRIzol (TRI reagent)","volume":"2010","author":"Rio","year":"2010","journal-title":"Cold Spring Harb. Protoc."},{"key":"10.1016\/j.fsi.2026.111504_bib51","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.ymeth.2009.12.006","article-title":"Why the need for qPCR publication guidelines?\u2014The case for MIQE","volume":"50","author":"Bustin","year":"2010","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2026.111504_bib52","doi-asserted-by":"crossref","first-page":"e93","DOI":"10.1093\/nar\/gng093","article-title":"Mathematics of quantitative kinetic PCR and the application of standard curves","volume":"31","author":"Rutledge","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.fsi.2026.111504_bib53","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/0003-2697(85)90442-7","article-title":"Measurement of protein using bicinchoninic acid","volume":"150","author":"Smith","year":"1985","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.fsi.2026.111504_bib54","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.fsi.2026.111504_bib55","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/B978-0-12-420119-4.00019-7","article-title":"Western blotting using chemiluminescent substrates","volume":"541","author":"Alegria-Schaffer","year":"2014","journal-title":"Methods Enzymol."},{"key":"10.1016\/j.fsi.2026.111504_bib56","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0278277","article-title":"An oral pH-responsive Streptococcus agalactiae vaccine formulation provides protective immunity to pathogen challenge in tilapia: a proof-of-concept study","volume":"18","author":"Bashir","year":"2023","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2026.111504_bib57","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.108684","article-title":"Immune response and protective efficacy of mannosylated polyethylenimine (PEI) as an antigen delivery vector, administered with a Streptococcus agalactiae DNA vaccine in Nile tilapia (Oreochromis niloticus)","volume":"135","author":"Chen","year":"2023","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111504_bib58","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.109353","article-title":"Immune response and protective efficacy of Streptococcus agalactiae vaccine coated with chitosan oligosaccharide for different immunization strategy in Nile tilapia (Oreochromis niloticus)","volume":"145","author":"Lu","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111504_bib59","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2^-\u0394\u0394Ct method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.fsi.2026.111504_bib60","doi-asserted-by":"crossref","first-page":"3769","DOI":"10.1016\/j.vaccine.2004.03.012","article-title":"Efficacy of Streptococcus agalactiae (group B) vaccine in tilapia (Oreochromis niloticus) by intraperitoneal and bath immersion administration","volume":"22","author":"Evans","year":"2004","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2026.111504_bib61","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1111\/jfd.12935","article-title":"Capsular polysaccharide of Streptococcus agalactiae is an essential virulence factor for infection in Nile tilapia (Oreochromis niloticus Linn.)","volume":"42","author":"Zhang","year":"2019","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2026.111504_bib62","doi-asserted-by":"crossref","first-page":"29","DOI":"10.3390\/vaccines8010029","article-title":"Surface immunogenic protein of Streptococcus group B is an agonist of toll-like receptors 2 and 4 and a potential immune adjuvant","volume":"8","author":"Diaz-Dinamarca","year":"2020","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.fsi.2026.111504_bib63","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1186188","article-title":"Surface immunogenic protein from Streptococcus agalactiae and Fissurella latimarginata hemocyanin are TLR4 ligands and activate MyD88- and TRIF-dependent signaling pathways","volume":"14","author":"D\u00edaz-Dinamarca","year":"2023","journal-title":"Front. Immunol."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826004080?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826004080?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T06:38:00Z","timestamp":1785911880000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464826004080"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":63,"alternative-id":["S1050464826004080"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111504","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Oral immunization of Nile tilapia with Chlorella-bioencapsulated multi-epitope fusion antigen effectively protects against Streptococcus agalactiae infection","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111504","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"111504"}}