{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T06:53:55Z","timestamp":1785912835147,"version":"3.56.0"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFF1000302"],"award-info":[{"award-number":["2022YFF1000302"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32273169"],"award-info":[{"award-number":["32273169"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.fsi.2026.111547","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T06:40:17Z","timestamp":1782456017000},"page":"111547","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Sexual dimorphism of antiviral immunity in grass carp (Ctenopharyngodon idella): TLR7 and TLR8b promote resistance to GCRV in males, whereas TLR8a increases susceptibility in females"],"prefix":"10.1016","volume":"176","author":[{"given":"Jingjing","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuezong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengxu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanyu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9083-0715","authenticated-orcid":false,"given":"Jianguo","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2026.111547_bib1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1146\/annurev-immunol-101320-125133","article-title":"Sex differences in immunity","volume":"40","author":"Wilkinson","year":"2022","journal-title":"Annu. Rev. Immunol."},{"issue":"2","key":"10.1016\/j.fsi.2026.111547_bib2","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1080\/23308249.2024.2390965","article-title":"Sex is a crucial factor in the immune response: an ichthyological perspective","volume":"33","author":"Caballero-Huertas","year":"2025","journal-title":"Rev. Fish. Sci. Aquacult."},{"issue":"1","key":"10.1016\/j.fsi.2026.111547_bib3","doi-asserted-by":"crossref","first-page":"9","DOI":"10.3390\/biology7010009","article-title":"Effects of sex steroids on fish leukocytes","volume":"7","author":"Chaves-Pozo","year":"2018","journal-title":"Biology"},{"key":"10.1016\/j.fsi.2026.111547_bib4","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1038\/nri.2016.90","article-title":"Sex differences in immune responses","volume":"16","author":"Klein","year":"2016","journal-title":"Nat. Rev. Immunol."},{"issue":"4","key":"10.1016\/j.fsi.2026.111547_bib5","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1038\/ni.2549","article-title":"Fishing for mammalian paradigms in the teleost immune system","volume":"14","author":"Sunyer","year":"2013","journal-title":"Nat. Immunol."},{"key":"10.1016\/j.fsi.2026.111547_bib6","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.dci.2012.04.001","article-title":"Advances in research of fish immune-relevant genes: a comparative overview of innate and adaptive immunity in teleosts","volume":"39","author":"Zhu","year":"2013","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2026.111547_bib7","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1186\/s12864-022-08503-x","article-title":"Chromosome-level genome assembly of grass carp (Ctenopharyngodon idella) provides insights into its genome evolution","volume":"23","author":"Wu","year":"2022","journal-title":"BMC Genom."},{"issue":"6","key":"10.1016\/j.fsi.2026.111547_bib8","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1038\/ng.3280","article-title":"The draft genome of the grass carp (Ctenopharyngodon idellus) provides insights into its evolution and vegetarian adaptation","volume":"47","author":"Wang","year":"2015","journal-title":"Nat. Genet."},{"key":"10.1016\/j.fsi.2026.111547_bib9","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1007\/s10126-023-10225-4","article-title":"Transcriptome analysis of the spleen provides insight into the immune regulation of GCRV resistance in grass carp (Ctenopharyngodon idella)","volume":"25","author":"Yu","year":"2023","journal-title":"Mar. Biotechnol."},{"key":"10.1016\/j.fsi.2026.111547_bib10","article-title":"Whole genome resequencing reveals grass carp candidate sex chromosome and sex determination system","volume":"48","author":"Zhang","year":"2026","journal-title":"Aquac. Rep."},{"key":"10.1016\/j.fsi.2026.111547_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2023.739931","article-title":"Comprehensive analysis of the immunological differences in the intestinal barrier of improved grass carp and their parents","volume":"577","author":"Tan","year":"2023","journal-title":"Aquaculture"},{"issue":"1","key":"10.1016\/j.fsi.2026.111547_bib12","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1111\/raq.12844","article-title":"Structure, function and immune evasion strategies of aquareoviruses, with focus on grass carp reovirus","volume":"16","author":"Li","year":"2024","journal-title":"Rev. Aquacult."},{"issue":"4","key":"10.1016\/j.fsi.2026.111547_bib13","article-title":"Seventy years of research advances in molecular epidemiology of grass carp reovirus in China, Acta Hydrobiol","volume":"50","author":"Liu","year":"2026","journal-title":"Sinica"},{"key":"10.1016\/j.fsi.2026.111547_bib14","doi-asserted-by":"crossref","DOI":"10.3390\/ijms242115501","article-title":"Comparative transcriptomic analysis revealed potential differential mechanisms of grass carp reovirus pathogenicity","volume":"24","author":"Kong","year":"2023","journal-title":"Int. J. Mol. Sci."},{"issue":"7","key":"10.1016\/j.fsi.2026.111547_bib15","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1111\/j.1462-2920.2006.01054.x","article-title":"Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment","volume":"8","author":"Cabello","year":"2006","journal-title":"Environ. Microbiol."},{"issue":"5","key":"10.1016\/j.fsi.2026.111547_bib16","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/ni.1863","article-title":"The role of pattern-recognition receptors in innate immunity: update on toll-like receptors","volume":"11","author":"Kawai","year":"2010","journal-title":"Nat. Immunol."},{"issue":"7","key":"10.1016\/j.fsi.2026.111547_bib17","doi-asserted-by":"crossref","first-page":"1889","DOI":"10.1007\/s11427-024-2822-5","article-title":"Toll-like receptor signaling in teleosts","volume":"68","author":"Su","year":"2025","journal-title":"Sci. China Life Sci."},{"issue":"27","key":"10.1016\/j.fsi.2026.111547_bib18","doi-asserted-by":"crossref","first-page":"9577","DOI":"10.1073\/pnas.0502272102","article-title":"The evolution of vertebrate toll-like receptors","volume":"102","author":"Roach","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"4","key":"10.1016\/j.fsi.2026.111547_bib19","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1002\/JLB.6RI0319-094R","article-title":"When the balance is broken: X-linked gene dosage from two X chromosomes and female-biased autoimmunity","volume":"106","author":"Syrett","year":"2019","journal-title":"J. Leukoc. Biol."},{"key":"10.1016\/j.fsi.2026.111547_bib20","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s00281-018-0712-y","article-title":"Female predisposition to TLR7-driven autoimmunity: gene dosage and the escape from X chromosome inactivation","volume":"41","author":"Souyris","year":"2019","journal-title":"Semin. Immunopathol."},{"key":"10.1016\/j.fsi.2026.111547_bib21","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s13293-023-00544-5","article-title":"TLR8 escapes X chromosome inactivation in human monocytes and CD4+ T cells","volume":"14","author":"Youness","year":"2023","journal-title":"Biol. Sex Differ."},{"key":"10.1016\/j.fsi.2026.111547_bib22","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2020.108485","article-title":"Heterogeneous escape from X chromosome inactivation results in sex differences in type I IFN responses at the single human pDC level","volume":"33","author":"Hagen","year":"2020","journal-title":"Cell Rep."},{"issue":"7837","key":"10.1016\/j.fsi.2026.111547_bib23","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1038\/s41586-020-2700-3","article-title":"Sex differences in immune responses that underlie COVID-19 disease outcomes","volume":"588","author":"Takahashi","year":"2020","journal-title":"Nature"},{"key":"10.1016\/j.fsi.2026.111547_bib24","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2025.1479814","article-title":"The toll like receptor 7 pathway and the sex bias of systemic lupus erythematosus","volume":"16","author":"Scofield","year":"2025","journal-title":"Front. Immunol."},{"issue":"2","key":"10.1016\/j.fsi.2026.111547_bib25","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.cellimm.2015.01.018","article-title":"Estrogen receptors regulate innate immune cells and signaling pathways","volume":"294","author":"Kovats","year":"2015","journal-title":"Cell. Immunol."},{"key":"10.1016\/j.fsi.2026.111547_bib26","article-title":"Male-biased Cyp17a2 orchestrates antiviral sexual dimorphism in fish via STING stabilization and viral protein degradation","volume":"14","author":"Lu","year":"2025","journal-title":"eLife"},{"issue":"6","key":"10.1016\/j.fsi.2026.111547_bib27","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1010626","article-title":"Fish female-biased gene cyp19a1a leads to female antiviral response attenuation between sexes by autophagic degradation of MITA","volume":"18","author":"Lu","year":"2022","journal-title":"PLoS Pathog."},{"issue":"13","key":"10.1016\/j.fsi.2026.111547_bib28","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1017\/S0031182015001286","article-title":"Demasculinization of male guppies increases resistance to a common and harmful ectoparasite","volume":"142","author":"Dargent","year":"2015","journal-title":"Parasitology"},{"issue":"10","key":"10.1016\/j.fsi.2026.111547_bib29","doi-asserted-by":"crossref","first-page":"2509","DOI":"10.1111\/evo.14266","article-title":"Male and female reproductive fitness costs of an immune response in natural populations","volume":"75","author":"De Lisle","year":"2021","journal-title":"Evolution"},{"key":"10.1016\/j.fsi.2026.111547_bib30","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1007\/s10126-011-9365-8","article-title":"Induced gynogenesis in grass carp (Ctenopharyngodon idellus) using irradiated sperm of allotetraploid hybrids","volume":"13","author":"Zhang","year":"2011","journal-title":"Mar. Biotechnol."},{"key":"10.1016\/j.fsi.2026.111547_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2026.743843","article-title":"Molecular characterization and expression patterns of TLR22b gene in grass carp (Ctenopharyngodon idella) and association of single nucleotide polymorphisms with resistance\/susceptibility to grass carp reovirus","volume":"619","author":"Zhang","year":"2026","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2026.111547_bib32","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.dci.2017.05.019","article-title":"Pattern recognition receptors in grass carp Ctenopharyngodon idella: I. Organization and expression analysis of TLRs and RLRs","volume":"76","author":"Liao","year":"2017","journal-title":"Dev. Comp. Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111547_bib33","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.molimm.2014.05.004","article-title":"RNA-seq profiles from grass carp tissues after reovirus (GCRV) infection based on singular and modular enrichment analyses","volume":"61","author":"Shi","year":"2014","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2026.111547_bib34","article-title":"Sex-dependent host-microbiome dynamics in zebrafish: implications for toxicology and gastrointestinal physiology","volume":"42","author":"Martyniuk","year":"2022","journal-title":"Comp. Biochem. Physiol. D: Genom. Proteonomics"},{"key":"10.1016\/j.fsi.2026.111547_bib35","doi-asserted-by":"crossref","first-page":"2525","DOI":"10.1016\/j.molimm.2010.06.009","article-title":"The fight between the teleost fish immune response and aquatic viruses","volume":"47","author":"Workenhe","year":"2010","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2026.111547_bib36","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.fsi.2004.09.006","article-title":"Innate immunity of fish (overview)","volume":"20","author":"Magnadottir","year":"2006","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111547_bib37","article-title":"Heterodimerization with signaling-inert TLR8b converts TLR8a from proviral to antiviral sensor in Ctenopharyngodon idella","author":"Zhang","year":"2026","journal-title":"Cell Commun. Signal."},{"key":"10.1016\/j.fsi.2026.111547_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2023.108315","article-title":"TLR7 neo-functionalizes to sense dsRNA and trigger antiviral and antibacterial immunity in non-tetrapod vertebrates","volume":"26","author":"Jiang","year":"2023","journal-title":"iScience"},{"key":"10.1016\/j.fsi.2026.111547_bib39","doi-asserted-by":"crossref","DOI":"10.1016\/j.virusres.2021.198525","article-title":"Novirhabdoviruses versus fish innate immunity: a review","volume":"304","author":"He","year":"2021","journal-title":"Virus Res."},{"key":"10.1016\/j.fsi.2026.111547_bib40","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.109921","article-title":"Sexual dimorphism in fish innate immunity: a functional and transcriptional study in yellowtail kingfish","volume":"154","author":"Valero","year":"2024","journal-title":"Fish Shellfish Immunol."},{"issue":"36","key":"10.1016\/j.fsi.2026.111547_bib41","doi-asserted-by":"crossref","first-page":"15838","DOI":"10.1073\/pnas.1001337107","article-title":"Sex-specific association of X-linked toll-like receptor 7 (TLR7) with male systemic lupus erythematosus","volume":"107","author":"Shen","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.fsi.2026.111547_bib42","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.3390\/ijms22031114","article-title":"Escape from X chromosome inactivation and the female predominance in autoimmune diseases","volume":"22","author":"Youness","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fsi.2026.111547_bib43","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1038\/s41598-017-01338-7","article-title":"Large-scale SNP screenings identify markers linked with GCRV resistant traits through transcriptomes of individuals and cell lines in Ctenopharyngodon idella","volume":"7","author":"Liao","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fsi.2026.111547_bib44","doi-asserted-by":"crossref","first-page":"4904","DOI":"10.1016\/j.jmb.2013.09.033","article-title":"The antiviral innate immune response in fish: evolution and conservation of the IFN system","volume":"425","author":"Langevin","year":"2013","journal-title":"J. Mol. Biol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111547_bib45","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1360\/SSV-2025-0090","article-title":"Investigating the application potential of TLR8a in disease-resistant breeding of Ctenopharyngodon idella via CRISPR\/Cas9","volume":"56","author":"Chen","year":"2026","journal-title":"Sci. Sin. Vitae"},{"key":"10.1016\/j.fsi.2026.111547_bib46","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.dci.2013.04.015","article-title":"Identification, expression profiling of a grass carp TLR8 and its inhibition leading to the resistance to reovirus in CIK cells","volume":"41","author":"Chen","year":"2013","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2026.111547_bib47","doi-asserted-by":"crossref","first-page":"134","DOI":"10.3390\/pathogens11020134","article-title":"The relevance of TLR8 in viral infections","volume":"11","author":"Martinez-Espinoza","year":"2022","journal-title":"Pathogens"},{"issue":"6","key":"10.1016\/j.fsi.2026.111547_bib48","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1007\/s00438-014-0889-2","article-title":"Whole-genome duplication in teleost fishes and its evolutionary consequences","volume":"289","author":"Glasauer","year":"2014","journal-title":"Mol. Genet. Genom."},{"key":"10.1016\/j.fsi.2026.111547_bib49","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.dci.2009.10.002","article-title":"Identification, characterization and genetic mapping of TLR7, TLR8a1 and TLR8a2 genes in rainbow trout (Oncorhynchus mykiss)","volume":"34","author":"Palti","year":"2010","journal-title":"Dev. Comp. Immunol."},{"issue":"5663","key":"10.1016\/j.fsi.2026.111547_bib50","doi-asserted-by":"crossref","first-page":"1526","DOI":"10.1126\/science.1093620","article-title":"Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8","volume":"303","author":"Heil","year":"2004","journal-title":"Science"}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826004511?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826004511?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T06:37:52Z","timestamp":1785911872000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464826004511"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":50,"alternative-id":["S1050464826004511"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111547","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Sexual dimorphism of antiviral immunity in grass carp (Ctenopharyngodon idella): TLR7 and TLR8b promote resistance to GCRV in males, whereas TLR8a increases susceptibility in females","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111547","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"111547"}}