{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T22:16:20Z","timestamp":1788300980875,"version":"build-2803163510"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2023MC208"],"award-info":[{"award-number":["ZR2023MC208"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017691","name":"Guangxi Key Research and Development Program","doi-asserted-by":"publisher","award":["Guike Nong AB2506910047"],"award-info":[{"award-number":["Guike Nong AB2506910047"]}],"id":[{"id":"10.13039\/501100017691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.fsi.2026.111648","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T15:17:35Z","timestamp":1786547855000},"page":"111648","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Molecular characterization of Fyn, Lck and Zap-70 in golden pompano (Trachinotus ovatus)"],"prefix":"10.1016","volume":"178","author":[{"given":"Youchuan","family":"Wei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongqiang","family":"Gan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenrui","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yushuai","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyun","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingzhu","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2249-9522","authenticated-orcid":false,"given":"Zhitao","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2026.111648_bib1","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2022.104621","article-title":"Regulatory roles of cytokines in T and B lymphocytes-mediated immunity in teleost fish","volume":"144","author":"Cao","year":"2023","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2026.111648_bib2","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s40168-026-02370-4","article-title":"Gut T cell-microbiota crosstalk orchestrates antibacterial immunity and mucosal homeostasis in teleost","volume":"14","author":"Geng","year":"2026","journal-title":"Microbiome"},{"key":"10.1016\/j.fsi.2026.111648_bib3","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1016\/j.fsi.2010.08.016","article-title":"T cell diversity and TcR repertoires in teleost fish","volume":"31","author":"Castro","year":"2011","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111648_bib4","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1038\/s41577-018-0020-8","article-title":"Regulatory mechanisms in T cell receptor signalling","volume":"18","author":"Gaud","year":"2018","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.fsi.2026.111648_bib5","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/S1074-7613(00)80499-9","article-title":"Alpha beta T cell development is abolished in mice lacking both Lck and Fyn protein tyrosine kinases","volume":"5","author":"van Oers","year":"1996","journal-title":"Immunity"},{"key":"10.1016\/j.fsi.2026.111648_bib6","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1146\/annurev-immunol-042617-053335","article-title":"ZAP-70 in signaling, biology, and disease","volume":"36","author":"Au-Yeung","year":"2018","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.fsi.2026.111648_bib7","doi-asserted-by":"crossref","first-page":"13","DOI":"10.24272\/j.issn.2095-8137.2023.053","article-title":"Full T-cell activation and function in teleosts require collaboration of first and co-stimulatory signals","volume":"45","author":"Liang","year":"2024","journal-title":"Zool. Res."},{"key":"10.1016\/j.fsi.2026.111648_bib8","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/0092-8674(94)90334-4","article-title":"Signal transduction by lymphocyte antigen receptors","volume":"76","author":"Weiss","year":"1994","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2026.111648_bib9","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/j.it.2003.08.003","article-title":"T-cell receptor signal transmission: who gives an ITAM?","volume":"24","author":"Pitcher","year":"2003","journal-title":"Trends Immunol."},{"key":"10.1016\/j.fsi.2026.111648_bib10","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/0092-8674(92)90598-7","article-title":"ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR zeta chain","volume":"71","author":"Chan","year":"1992","journal-title":"Cell"},{"key":"10.1016\/j.fsi.2026.111648_bib11","doi-asserted-by":"crossref","DOI":"10.1155\/2022\/9112127","article-title":"Fyn signaling in ischemia-reperfusion injury: potential and therapeutic implications","volume":"2022","author":"Du","year":"2022","journal-title":"Mediat. Inflamm."},{"key":"10.1016\/j.fsi.2026.111648_bib12","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-17021-w","article-title":"Lck is a relevant target in chronic lymphocytic leukaemia cells whose expression variance is unrelated to disease outcome","volume":"7","author":"Till","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fsi.2026.111648_bib13","doi-asserted-by":"crossref","first-page":"3629","DOI":"10.1182\/blood.2020009960","article-title":"ZAP-70 constitutively regulates gene expression and protein synthesis in chronic lymphocytic leukemia","volume":"137","author":"Chen","year":"2021","journal-title":"Blood"},{"key":"10.1016\/j.fsi.2026.111648_bib14","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.fsi.2017.03.016","article-title":"Grouper (Epinephelus coioides) TCR signaling pathway was involved in response against Cryptocaryon irritans infection","volume":"64","author":"Li","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111648_bib15","doi-asserted-by":"crossref","first-page":"2376","DOI":"10.1007\/s12010-014-1443-8","article-title":"Molecular characterization and expression of Lck in Nile tilapia (Oreochromis niloticus) in response to Streptococcus agalactiae stimulus","volume":"175","author":"Gan","year":"2015","journal-title":"Appl. Biochem. Biotechnol."},{"key":"10.1016\/j.fsi.2026.111648_bib16","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1007\/s13258-015-0372-4","article-title":"Molecular characterization and expression of ZAP-70 in Nile tilapia (Oreochromis niloticus) in response to Streptococcus agalactiae stimulus","volume":"38","author":"Gan","year":"2016","journal-title":"Genes Genom."},{"key":"10.1016\/j.fsi.2026.111648_bib17","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1016\/j.fsi.2010.08.011","article-title":"Cloning, characterization, and expression pattern of Atlantic halibut (Hippoglossus hippoglossus L.) CD4-2, Lck, and ZAP-70","volume":"29","author":"Overgard","year":"2010","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111648_bib18","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1111\/jfd.12414","article-title":"Molecular cloning and characterization of lymphocyte cell kinase from humphead snapper (Lutjanus sanguineus)","volume":"39","author":"Huang","year":"2016","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2026.111648_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2025.105520","article-title":"Structural characterization of T-cell kinases Lck1 and ZAP-70 in rainbow trout (Oncorhynchus mykiss) through homology modeling","volume":"173","author":"Bravo","year":"2025","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2026.111648_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.micpath.2022.105689","article-title":"Characterization of the pathogenesis and immune response to a highly virulent Edwardsiella tarda strain responsible for mass mortality in the hybrid snakehead (Channa maculate \u2640 x Channa argus \u2642)","volume":"170","author":"Guo","year":"2022","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.fsi.2026.111648_bib21","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2025.105529","article-title":"Characterization of the CD28 signaling pathway related genes (CD28, Grb2 and SLP-76) of golden pompano (Trachinotus ovatus)","volume":"173","author":"Qi","year":"2025","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2026.111648_bib22","article-title":"Two non-mammalian toll-like receptors (TLR21 and TLR22) from golden pompano (Trachinotus ovatus): molecular cloning, gene characterization and expression analysis","volume":"21","author":"Gao","year":"2021","journal-title":"Aquac. Rep."},{"key":"10.1016\/j.fsi.2026.111648_bib23","article-title":"Molecular characterization and functional analysis of DIGIRR from golden pompano (Trachinotus ovatus)","volume":"13","author":"Xie","year":"2022","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2026.111648_bib24","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA 7: molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.fsi.2026.111648_bib25","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT-PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.fsi.2026.111648_bib26","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1126\/science.aad9964","article-title":"Phase separation of signaling molecules promotes T cell receptor signal transduction","volume":"352","author":"Su","year":"2016","journal-title":"Science"},{"key":"10.1016\/j.fsi.2026.111648_bib27","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1146\/annurev.cellbio.13.1.513","article-title":"Cellular functions regulated by Src family kinases","volume":"13","author":"Thomas","year":"1997","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"10.1016\/j.fsi.2026.111648_bib28","doi-asserted-by":"crossref","first-page":"R85","DOI":"10.1530\/REP-18-0440","article-title":"Emerging role for SRC family kinases in junction dynamics during spermatogenesis","volume":"157","author":"Xiao","year":"2019","journal-title":"Reproduction"},{"key":"10.1016\/j.fsi.2026.111648_bib29","doi-asserted-by":"crossref","first-page":"32878","DOI":"10.1074\/jbc.273.49.32878","article-title":"Zinc is essential for binding of p56 (lck) to CD4 and CD8 alpha","volume":"273","author":"Lin","year":"1998","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fsi.2026.111648_bib30","doi-asserted-by":"crossref","first-page":"3226","DOI":"10.4049\/jimmunol.165.6.3226","article-title":"Serine 6 of Lck tyrosine kinase: a critical site for Lck myristoylation, membrane localization, and function in T lymphocytes","volume":"165","author":"Yasuda","year":"2000","journal-title":"J. Immunol."},{"key":"10.1016\/j.fsi.2026.111648_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2024.741615","article-title":"TLRs signaling pathway regulation, antibacterial and apoptotic activity of largemouth bass ECSIT during Edwardsiella piscicida infection","volume":"595","author":"Pi","year":"2025","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2026.111648_bib32","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.bcp.2017.11.015","article-title":"Src family kinase tyrosine phosphorylates toll-like receptor 4 to dissociate MyD88 and Mal\/Tirap, suppressing LPS-induced inflammatory responses","volume":"147","author":"Mitchell","year":"2018","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.fsi.2026.111648_bib33","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.dci.2017.04.004","article-title":"Molecular cloning, structural modeling, and expression analysis of MyD88 and IRAK4 of golden pompano (Trachinotus ovatus)","volume":"74","author":"Qi","year":"2017","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2026.111648_bib34","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.fsi.2017.02.026","article-title":"Molecular cloning and expression analysis of toll-like receptor genes (TLR7, TLR8 and TLR9) of golden pompano (Trachinotus ovatus)","volume":"63","author":"Wei","year":"2017","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111648_bib35","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0097471","article-title":"De novo characterization of the spleen transcriptome of the large yellow croaker (Pseudosciaena crocea) and analysis of the immune relevant genes and pathways involved in the antiviral response","volume":"9","author":"Mu","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2026.111648_bib36","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1084\/jem.179.4.1163","article-title":"p56lck interacts via its src homology 2 domain with the ZAP-70 kinase","volume":"179","author":"Duplay","year":"1994","journal-title":"J. Exp. Med."},{"key":"10.1016\/j.fsi.2026.111648_bib37","doi-asserted-by":"crossref","first-page":"14229","DOI":"10.1074\/jbc.274.20.14229","article-title":"Tyrosine 319 in the interdomain B of Zap-70 is a binding site for the Src homology 2 domain of Lck","volume":"274","author":"Pelosi","year":"1999","journal-title":"J. Biol. Chem."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826005528?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826005528?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T21:58:33Z","timestamp":1788299913000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464826005528"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":37,"alternative-id":["S1050464826005528"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111648","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular characterization of Fyn, Lck and Zap-70 in golden pompano (Trachinotus ovatus)","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111648","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"111648"}}