{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T22:16:54Z","timestamp":1788301014344,"version":"build-2803163510"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32273169"],"award-info":[{"award-number":["32273169"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017691","name":"Guangxi Key Research and Development Program","doi-asserted-by":"publisher","award":["Guikenong AB2506910047"],"award-info":[{"award-number":["Guikenong AB2506910047"]}],"id":[{"id":"10.13039\/501100017691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.fsi.2026.111666","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:17:50Z","timestamp":1787239070000},"page":"111666","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Safety, immunogenicity, and broad-spectrum protective efficacy of a naturally attenuated GCRV-II strain against viral and bacterial challenges in grass carp"],"prefix":"10.1016","volume":"178","author":[{"given":"Dingzhe","family":"Zou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wangqian","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5429-0748","authenticated-orcid":false,"given":"Run","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyu","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yucheng","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1770-957X","authenticated-orcid":false,"given":"Weiwei","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2026.111666_bib1","series-title":"China Fishery Statistical Yearbook","author":"Fisheries","year":"2025"},{"issue":"4","key":"10.1016\/j.fsi.2026.111666_bib2","first-page":"168","article-title":"Seventy years of research progress in the molecular epidemiology of grass carp reovirus in China","volume":"50","author":"Man","year":"2026","journal-title":"Acta Hydrobiol. Sin."},{"issue":"Pt 8","key":"10.1016\/j.fsi.2026.111666_bib3","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1099\/0022-1317-83-8-1941","article-title":"Common evolutionary origin of aquareoviruses and orthoreoviruses revealed by genome characterization of Golden shiner reovirus, Grass carp reovirus, striped bass reovirus and golden ide reovirus (genus Aquareovirus, family Reoviridae)","volume":"83","author":"Attoui","year":"2002","journal-title":"J. Gen. Virol."},{"issue":"6","key":"10.1016\/j.fsi.2026.111666_bib4","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1360\/062004-105","article-title":"3D reconstruction and capsid protein characterization of grass carp reovirus, Science in China","volume":"48","author":"Fang","year":"2005","journal-title":"Series C, Life Sci."},{"issue":"22","key":"10.1016\/j.fsi.2026.111666_bib5","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.02333-12","article-title":"Complete genome sequence of a reovirus isolated from grass carp, indicating different genotypes of GCRV in China","volume":"86","author":"Wang","year":"2012","journal-title":"J. Virol."},{"issue":"Pt 10","key":"10.1016\/j.fsi.2026.111666_bib6","doi-asserted-by":"crossref","first-page":"2266","DOI":"10.1099\/vir.0.054767-0","article-title":"Identification and genomic characterization of a novel fish reovirus, Hubei grass carp disease reovirus, isolated in 2009 in China","volume":"94","author":"Fan","year":"2013","journal-title":"J. Gen. Virol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111666_bib7","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.virusres.2011.10.014","article-title":"Complete genomic sequence of a reovirus isolated from grass carp in China","volume":"163","author":"Ye","year":"2012","journal-title":"Virus Res."},{"key":"10.1016\/j.fsi.2026.111666_bib8","doi-asserted-by":"crossref","DOI":"10.1111\/jfd.70188","article-title":"Protective efficacy of vaccines against grass carp hemorrhagic disease: a systematic review and meta-analysis","author":"Liu","year":"2026","journal-title":"J. Fish. Dis."},{"key":"10.1016\/j.fsi.2026.111666_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.109160","article-title":"Recombinant surface display vaccine enhances the immersion immune effect against grass carp reovirus in grass carp (Ctenopharyngodon idella)","volume":"142","author":"Hao","year":"2023","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111666_bib10","series-title":"Studies on Attenuation of Grass Carp Hemorrhagic Disease Virus","first-page":"211","author":"Xu","year":"1998"},{"key":"10.1016\/j.fsi.2026.111666_bib11","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.fsi.2018.01.047","article-title":"Comparative study of the immunoprotective effect of two DNA vaccines against grass carp reovirus","volume":"75","author":"Chen","year":"2018","journal-title":"Fish Shellfish Immunol."},{"issue":"25","key":"10.1016\/j.fsi.2026.111666_bib12","doi-asserted-by":"crossref","first-page":"3613","DOI":"10.1016\/j.vaccine.2018.05.043","article-title":"Plasmid pcDNA3.1-s11 constructed based on the S11 segment of grass carp reovirus as DNA vaccine provides immune protection","volume":"36","author":"Gao","year":"2018","journal-title":"Vaccine"},{"key":"10.1016\/j.fsi.2026.111666_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.lfs.2023.122331","article-title":"Vaccine development: current trends and technologies","volume":"336","author":"Poria","year":"2024","journal-title":"Life Sci."},{"issue":"1","key":"10.1016\/j.fsi.2026.111666_bib14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s13337-013-0186-4","article-title":"Viral vaccines for farmed finfish","volume":"25","author":"Dhar","year":"2014","journal-title":"Virusdisease"},{"issue":"4","key":"10.1016\/j.fsi.2026.111666_bib15","first-page":"635","article-title":"The double-edged sword: how evolution can make or break a live-attenuated virus vaccine","volume":"4","author":"Hanley","year":"2011","journal-title":"Evolution"},{"issue":"6","key":"10.1016\/j.fsi.2026.111666_bib16","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1038\/nbt.1635","article-title":"Rationalizing the development of live attenuated virus vaccines","volume":"28","author":"Lauring","year":"2010","journal-title":"Nat. Biotechnol."},{"issue":"12","key":"10.1016\/j.fsi.2026.111666_bib17","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.01390-25","article-title":"Live-attenuated influenza virus vaccine strain with an engineered temperature-sensitive and genetically stable viral polymerase variant","volume":"99","author":"Naito","year":"2025","journal-title":"J. Virol."},{"issue":"11","key":"10.1016\/j.fsi.2026.111666_bib18","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.01289-23","article-title":"Low-temperature immunization attenuates the residual virulence of orf074r gene-deleted infectious spleen and kidney necrosis virus: a candidate immersion vaccine","volume":"97","author":"Zeng","year":"2023","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2026.111666_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.108826","article-title":"An attenuated strain of cyprinid herpesvirus 2 as a vaccine candidate against herpesviral hematopoietic necrosis disease in gibel carp, Carassius auratus gibelio","volume":"138","author":"Sun","year":"2023","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111666_bib20","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.109871","article-title":"A naturaly attenuated largemouth bass ranavirus strain provided protection for Micropterus salmoides by immersion immunization","volume":"153","author":"Fu","year":"2024","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111666_bib21","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.fsi.2018.01.050","article-title":"Novel subunit vaccine based on grass carp reovirus VP35 protein provides protective immunity against grass carp hemorrhagic disease","volume":"75","author":"Gao","year":"2018","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111666_bib22","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1016\/j.fsi.2018.10.008","article-title":"Oral delivery of Bacillus subtilis spores expressing grass carp reovirus VP4 protein produces protection against grass carp reovirus infection","volume":"84","author":"Jiang","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111666_bib23","article-title":"Recombinant Lactococcus lactis expressing grass carp reovirus VP6 induces mucosal immunity against grass carp reovirus infection","volume":"13","author":"Wang","year":"2022","journal-title":"Front. Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111666_bib24","doi-asserted-by":"crossref","DOI":"10.3390\/vaccines9010041","article-title":"Oral vaccination of grass carp (Ctenopharyngodon idella) with baculovirus-expressed grass carp reovirus (GCRV) proteins induces protective immunity against GCRV infection","volume":"9","author":"Mu","year":"2021","journal-title":"Vaccines"},{"issue":"4","key":"10.1016\/j.fsi.2026.111666_bib25","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1007\/s00705-008-0048-3","article-title":"Characterization of infectious particles of grass carp reovirus by treatment with proteases","volume":"153","author":"Fang","year":"2008","journal-title":"Arch. Virol."},{"key":"10.1016\/j.fsi.2026.111666_bib26","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.fsi.2017.10.060","article-title":"Display of GCRV vp7 protein on the surface of Escherichia coli and its immunoprotective effects in grass carp (Ctenopharyngodon idella)","volume":"72","author":"Hao","year":"2018","journal-title":"Fish Shellfish Immunol."},{"issue":"2","key":"10.1016\/j.fsi.2026.111666_bib27","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.fsi.2014.09.014","article-title":"Single-walled carbon nanotubes as candidate recombinant subunit vaccine carrier for immunization of grass carp against grass carp reovirus","volume":"41","author":"Zhu","year":"2014","journal-title":"Fish Shellfish Immunol."},{"issue":"4","key":"10.1016\/j.fsi.2026.111666_bib28","doi-asserted-by":"crossref","first-page":"834","DOI":"10.4103\/jcrt.JCRT_1241_20","article-title":"Cancer immunotherapy: recent advances and challenges","volume":"17","author":"Dhar","year":"2021","journal-title":"J. Cancer Res. Therapeut."},{"issue":"7","key":"10.1016\/j.fsi.2026.111666_bib29","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/S0969-2126(96)00084-6","article-title":"Poliovirus biology","volume":"4","author":"Minor","year":"1996","journal-title":"Structure (London, England : 1993)"},{"issue":"22","key":"10.1016\/j.fsi.2026.111666_bib30","doi-asserted-by":"crossref","DOI":"10.3390\/ani14223289","article-title":"Natural Co-Infections of Aeromonas veronii and yellow catfish calicivirus (YcCV) in ascites disease outbreaks in cultured yellow catfish: an emerging fish disease in China","volume":"14","author":"Xu","year":"2024","journal-title":"Animals : Open Access J. MDPI"},{"key":"10.1016\/j.fsi.2026.111666_bib31","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.fsi.2020.07.012","article-title":"Impact of Aeromonas hydrophila and infectious spleen and kidney necrosis virus infections on susceptibility and host immune response in Chinese perch (Siniperca chuatsi)","volume":"105","author":"Liu","year":"2020","journal-title":"Fish Shellfish Immunol."},{"issue":"12","key":"10.1016\/j.fsi.2026.111666_bib32","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.1111\/jfd.12890","article-title":"Development of a VP38 recombinant protein-based indirect ELISA for detection of antibodies against grass carp reovirus genotype II (iELISA for detection of antibodies against GCRV II)","volume":"41","author":"Zeng","year":"2018","journal-title":"J. Fish. Dis."},{"issue":"6","key":"10.1016\/j.fsi.2026.111666_bib33","doi-asserted-by":"crossref","first-page":"1683","DOI":"10.1016\/j.fsi.2013.09.031","article-title":"A history of fish vaccination: science-based disease prevention in aquaculture","volume":"35","author":"Gudding","year":"2013","journal-title":"Fish Shellfish Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111666_bib34","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1586\/14760584.4.1.89","article-title":"Vaccines for fish in aquaculture","volume":"4","author":"Sommerset","year":"2005","journal-title":"Expert Rev. Vaccine"},{"issue":"12","key":"10.1016\/j.fsi.2026.111666_bib35","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1016\/j.dci.2011.03.006","article-title":"Toll-like receptors in bony fish: from genomics to function","volume":"35","author":"Palti","year":"2011","journal-title":"Dev. Comp. Immunol."},{"issue":"12","key":"10.1016\/j.fsi.2026.111666_bib36","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1016\/j.dci.2011.03.033","article-title":"Cytotoxic T cells in teleost fish","volume":"35","author":"Nakanishi","year":"2011","journal-title":"Dev. Comp. Immunol."},{"issue":"3","key":"10.1016\/j.fsi.2026.111666_bib37","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1007\/s00430-015-0388-z","article-title":"The history of vaccination against cytomegalovirus","volume":"204","author":"Plotkin","year":"2015","journal-title":"Med. Microbiol. Immunol."},{"key":"10.1016\/j.fsi.2026.111666_bib38","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.fsi.2018.03.060","article-title":"Correlates of protective immunity for fish vaccines","volume":"85","author":"Munang'andu","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111666_bib39","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.fsi.2020.02.004","article-title":"Grass carp reovirus VP56 represses interferon production by degrading phosphorylated IRF7","volume":"99","author":"Zhang","year":"2020","journal-title":"Fish Shellfish Immunol."},{"issue":"3","key":"10.1016\/j.fsi.2026.111666_bib40","first-page":"33+36","article-title":"The first live attenuated grass carp hemorrhagic disease vaccine successfully launched \u2014 modern fisheries news","volume":"26","author":"Yinmei","year":"2011"},{"issue":"1","key":"10.1016\/j.fsi.2026.111666_bib41","doi-asserted-by":"crossref","DOI":"10.3390\/vaccines9010053","article-title":"Recombinant baculovirus-produced grass carp reovirus virus-like particles as vaccine candidate that provides protective immunity against GCRV genotype II infection in grass carp","volume":"9","author":"Gao","year":"2021","journal-title":"Vaccines"},{"key":"10.1016\/j.fsi.2026.111666_bib42","series-title":"Grass Carp Reovirus: Molecular Epidemiology, Whole-Genome Sequence, and Studies on Inactivated Vaccines Based on Circulating Strains","author":"Zeng","year":"2017"},{"key":"10.1016\/j.fsi.2026.111666_bib43","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2023.104893","article-title":"Comparative transcriptional analysis between virulent isolate HN1307 and avirulent isolate GD1108 of grass carp reovirus genotype II","volume":"147","author":"Wang","year":"2023","journal-title":"Dev. Comp. Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111666_bib44","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1111\/jfd.12902","article-title":"Assessment of cross-protection to heterologous strains of Flavobacterium psychrophilum following vaccination with a live-attenuated coldwater disease immersion vaccine","volume":"42","author":"Ma","year":"2019","journal-title":"J. Fish. Dis."},{"issue":"Pt A","key":"10.1016\/j.fsi.2026.111666_bib45","article-title":"Co-infections of Aeromonas veronii and Nocardia seriolae in largemouth bass (Micropterus salmoides)","volume":"173","author":"Xu","year":"2022","journal-title":"Microb. Pathog."},{"issue":"6","key":"10.1016\/j.fsi.2026.111666_bib46","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1038\/s41577-020-0285-6","article-title":"Defining trained immunity and its role in health and disease","volume":"20","author":"Netea","year":"2020","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.fsi.2026.111666_bib47","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.dci.2016.03.003","article-title":"Long-lived effects of administering \u03b2-glucans: indications for trained immunity in fish","volume":"64","author":"Petit","year":"2016","journal-title":"Dev. Comp. Immunol."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S105046482600570X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S105046482600570X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T22:01:04Z","timestamp":1788300064000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S105046482600570X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":47,"alternative-id":["S105046482600570X"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111666","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Safety, immunogenicity, and broad-spectrum protective efficacy of a naturally attenuated GCRV-II strain against viral and bacterial challenges in grass carp","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111666","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"111666"}}