{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T03:36:09Z","timestamp":1792985769325,"version":"4.5.0"},"reference-count":52,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32173005"],"award-info":[{"award-number":["32173005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD2400702"],"award-info":[{"award-number":["2023YFD2400702"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFD0900503"],"award-info":[{"award-number":["2018YFD0900503"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.fsi.2026.111744","type":"journal-article","created":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:25:22Z","timestamp":1791008722000},"page":"111744","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Construction of red-spotted grouper nervous necrosis virus (RGNNV) nonstructural protein B1\/B2 mutant strains and dynamics during infection"],"prefix":"10.1016","volume":"179","author":[{"given":"Yuqi","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqian","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3365-1259","authenticated-orcid":false,"given":"Xiuzhen","family":"Sheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Chi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuang","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbin","family":"Zhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"3","key":"10.1016\/j.fsi.2026.111744_bib1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1046\/j.1365-2761.2002.00350.x","article-title":"Betanodavirus infections of teleost fish: a review","volume":"25","author":"Munday","year":"2002","journal-title":"J. Fish. Dis."},{"issue":"2","key":"10.1016\/j.fsi.2026.111744_bib2","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens9020106","article-title":"Betanodavirus and VER disease: a 30-year research review","volume":"9","author":"Bandin","year":"2020","journal-title":"Pathogens"},{"issue":"Pt 10","key":"10.1016\/j.fsi.2026.111744_bib3","doi-asserted-by":"crossref","first-page":"2807","DOI":"10.1099\/vir.0.80902-0","article-title":"Characterization of striped jack nervous necrosis virus subgenomic RNA3 and biological activities of its encoded protein B2","volume":"86","author":"Iwamoto","year":"2005","journal-title":"J. Gen. Virol."},{"issue":"2","key":"10.1016\/j.fsi.2026.111744_bib4","doi-asserted-by":"crossref","first-page":"97","DOI":"10.3354\/dao02015","article-title":"B2 protein from betanodavirus is expressed in recently infected but not in chronically infected fish","volume":"83","author":"Mezeth","year":"2009","journal-title":"Dis Aquat Organ"},{"issue":"4","key":"10.1016\/j.fsi.2026.111744_bib5","doi-asserted-by":"crossref","first-page":"1633","DOI":"10.1128\/aem.63.4.1633-1636.1997","article-title":"Genomic classification of fish nodaviruses by molecular phylogenetic analysis of the coat protein gene","volume":"63","author":"Nishizawa","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"issue":"10","key":"10.1016\/j.fsi.2026.111744_bib6","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1111\/j.1365-2761.2004.00581.x","article-title":"Characterization of nodavirus and viral encephalopathy and retinopathy in farmed turbot, Scophthalmus maximus (L.)","volume":"27","author":"Johansen","year":"2004","journal-title":"J. Fish. Dis."},{"issue":"1","key":"10.1016\/j.fsi.2026.111744_bib7","doi-asserted-by":"crossref","first-page":"3079","DOI":"10.1038\/s41598-018-21340-x","article-title":"Nuclear targeting of the betanodavirus B1 protein via two arginine-rich domains induces G1\/S cell cycle arrest mediated by upregulation of p53\/p21","volume":"8","author":"Su","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fsi.2026.111744_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.108578","article-title":"Red-grouper nervous necrosis virus B1 protein inhibits fish IFN response by targeting Ser5-phosphorylated RNA polymerase II to promote viral replication","volume":"134","author":"Qin","year":"2023","journal-title":"Fish Shellfish Immunol."},{"issue":"2","key":"10.1016\/j.fsi.2026.111744_bib9","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/j.virol.2008.11.048","article-title":"Betanodavirus non-structural protein B1: a novel anti-necrotic death factor that modulates cell death in early replication cycle in fish cells","volume":"385","author":"Chen","year":"2009","journal-title":"Virology"},{"issue":"14","key":"10.1016\/j.fsi.2026.111744_bib10","doi-asserted-by":"crossref","first-page":"6822","DOI":"10.1128\/JVI.00079-06","article-title":"Sequestration and protection of double-stranded RNA by the betanodavirus b2 protein","volume":"80","author":"Fenner","year":"2006","journal-title":"J. Virol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111744_bib11","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1128\/JVI.80.1.85-94.2006","article-title":"Betanodavirus B2 is an RNA interference antagonist that facilitates intracellular viral RNA accumulation","volume":"80","author":"Fenner","year":"2006","journal-title":"J. Virol."},{"issue":"11","key":"10.1016\/j.fsi.2026.111744_bib12","doi-asserted-by":"crossref","first-page":"5449","DOI":"10.1128\/JVI.00009-07","article-title":"Dissection of double-stranded RNA binding protein B2 from betanodavirus","volume":"81","author":"Fenner","year":"2007","journal-title":"J. Virol."},{"issue":"51","key":"10.1016\/j.fsi.2026.111744_bib13","doi-asserted-by":"crossref","first-page":"39801","DOI":"10.1074\/jbc.M110.164988","article-title":"Betanodavirus B2 causes ATP depletion-induced cell death via mitochondrial targeting and complex II inhibition in vitro and in vivo","volume":"285","author":"Su","year":"2010","journal-title":"J. Biol. Chem."},{"issue":"10","key":"10.1016\/j.fsi.2026.111744_bib14","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1007\/s10495-014-1016-x","article-title":"Beta-nodavirus B2 protein induces hydrogen peroxide production, leading to Drp1-recruited mitochondrial fragmentation and cell death via mitochondrial targeting","volume":"19","author":"Su","year":"2014","journal-title":"Apoptosis"},{"key":"10.1016\/j.fsi.2026.111744_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2021.736488","article-title":"Red-grouper nervous necrosis virus B2 protein negatively regulates fish interferon response by suppressing host transcription directed by RNA polymerase II","volume":"536","author":"Qin","year":"2021","journal-title":"Aquaculture"},{"issue":"5","key":"10.1016\/j.fsi.2026.111744_bib16","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.bsheal.2022.08.001","article-title":"Reverse genetics in virology: a double edged sword","volume":"4","author":"Chen","year":"2022","journal-title":"Biosafety and Health"},{"issue":"4","key":"10.1016\/j.fsi.2026.111744_bib17","doi-asserted-by":"crossref","first-page":"2326","DOI":"10.1128\/jvi.66.4.2326-2334.1992","article-title":"Replication of nodamura virus after transfection of viral RNA into mammalian cells in culture","volume":"66","author":"Ball","year":"1992","journal-title":"J. Virol."},{"issue":"24","key":"10.1016\/j.fsi.2026.111744_bib18","doi-asserted-by":"crossref","first-page":"12220","DOI":"10.1128\/JVI.75.24.12220-12227.2001","article-title":"Recovery of infectious pariacoto virus from cDNA clones and identification of susceptible cell lines","volume":"75","author":"Johnson","year":"2001","journal-title":"J. Virol."},{"issue":"2","key":"10.1016\/j.fsi.2026.111744_bib19","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1006\/viro.2002.1769","article-title":"Recovery of infectivity from cDNA clones of nodamura virus and identification of small nonstructural proteins","volume":"305","author":"Johnson","year":"2003","journal-title":"Virology"},{"issue":"Pt 11","key":"10.1016\/j.fsi.2026.111744_bib20","doi-asserted-by":"crossref","first-page":"2653","DOI":"10.1099\/0022-1317-82-11-2653","article-title":"Establishment of an infectious RNA transcription system for striped jack nervous necrosis virus, the type species of the betanodaviruses","volume":"82","author":"Iwamoto","year":"2001","journal-title":"J. Gen. Virol."},{"issue":"2","key":"10.1016\/j.fsi.2026.111744_bib21","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.jviromet.2008.04.002","article-title":"Establishment of reverse genetics system of betanodavirus for the efficient recovery of infectious particles","volume":"151","author":"Takizawa","year":"2008","journal-title":"J. Virol. Methods"},{"issue":"6","key":"10.1016\/j.fsi.2026.111744_bib22","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1099\/jgv.0.000830","article-title":"A single amino acid change in the non-structural NV protein impacts the virulence phenotype of Viral hemorrhagic septicemia virus in trout","volume":"98","author":"Baillon","year":"2017","journal-title":"J. Gen. Virol."},{"issue":"10","key":"10.1016\/j.fsi.2026.111744_bib23","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1099\/jgv.0.001130","article-title":"Deletion of accessory genes 3a, 3b, 5a or 5b from avian coronavirus infectious bronchitis virus induces an attenuated phenotype both in vitro and in vivo","volume":"99","author":"Laconi","year":"2018","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.fsi.2026.111744_bib24","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2020.735663","article-title":"Identification and characterization of a new strain of nervous necrosis virus isolated from pearl gentian grouper (Epinephelus lanceolatus \u00d7 Epinephelus fuscoguttatus) in China","volume":"529","author":"Xing","year":"2020","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2026.111744_bib25","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.fsi.2022.10.004","article-title":"CD4-1 and CD8\u03b1 T lymphocytes subsets in spotted sea bass (Lateolabrax maculatus) and comparison on antigenicity of T lymphocytes subsets in other three marine fish species","volume":"131","author":"Jiang","year":"2022","journal-title":"Fish Shellfish Immunol."},{"issue":"9","key":"10.1016\/j.fsi.2026.111744_bib26","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.00901-24","article-title":"Nectin1 is a pivotal host factor involved in attachment and entry of red-spotted grouper nervous necrosis virus in the early stages of the viral life cycle","volume":"98","author":"Zhang","year":"2024","journal-title":"J. Virol."},{"issue":"12","key":"10.1016\/j.fsi.2026.111744_bib27","doi-asserted-by":"crossref","DOI":"10.3390\/v14122818","article-title":"Characterization of Nervous Necrosis Virus (NNV) nonstructural protein B2 and its enhancement on virus proliferation","volume":"14","author":"Zhang","year":"2022","journal-title":"Viruses"},{"issue":"Pt 6","key":"10.1016\/j.fsi.2026.111744_bib28","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1099\/vir.0.000064","article-title":"In vitro and in vivo characterization of molecular determinants of virulence in reassortant betanodavirus","volume":"96","author":"Souto","year":"2015","journal-title":"J. Gen. Virol."},{"issue":"2","key":"10.1016\/j.fsi.2026.111744_bib29","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1111\/jfd.12916","article-title":"Amino acid changes in the capsid protein of a reassortant betanodavirus strain: effect on viral replication in vitro and in vivo","volume":"42","author":"Souto","year":"2019","journal-title":"J. Fish. Dis."},{"issue":"1","key":"10.1016\/j.fsi.2026.111744_bib30","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.01748-22","article-title":"Identification of B-Cell epitopes on capsid protein reveals two potential neutralization mechanisms in red-spotted grouper nervous necrosis virus","volume":"97","author":"Zhang","year":"2023","journal-title":"J. Virol."},{"issue":"1\u20132","key":"10.1016\/j.fsi.2026.111744_bib31","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.vetmic.2011.06.029","article-title":"Tissue distribution of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) genome in experimentally infected juvenile European seabass (Dicentrarchus labrax)","volume":"154","author":"Lopez-Jimena","year":"2011","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.fsi.2026.111744_bib32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.aquaculture.2018.01.042","article-title":"Distribution of nervous necrosis virus (NNV) in infected sevenband grouper, Hyporthodus septemfasciatus by intramuscular injection or immersion challenge","volume":"489","author":"Kim","year":"2018","journal-title":"Aquaculture"},{"issue":"8","key":"10.1016\/j.fsi.2026.111744_bib33","doi-asserted-by":"crossref","DOI":"10.3390\/v14081737","article-title":"Construction of attenuated strains for Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) via reverse genetic System","volume":"14","author":"Lei","year":"2022","journal-title":"Viruses"},{"issue":"1","key":"10.1016\/j.fsi.2026.111744_bib34","doi-asserted-by":"crossref","DOI":"10.1186\/s13567-025-01515-x","article-title":"Development of a reverse genetics system for Getah virus and characterization of rescued strains","volume":"56","author":"Cai","year":"2025","journal-title":"Vet. Res."},{"issue":"7","key":"10.1016\/j.fsi.2026.111744_bib35","article-title":"Two mutations in NS2B are responsible for attenuation of the yellow fever virus (YFV) vaccine strain 17D","volume":"21","author":"Gamarnik","year":"2025","journal-title":"PLoS Pathog."},{"issue":"7","key":"10.1016\/j.fsi.2026.111744_bib36","doi-asserted-by":"crossref","DOI":"10.3390\/microorganisms13071579","article-title":"Rotavirus reverse genetics systems and oral vaccine delivery vectors for mucosal vaccination","volume":"13","author":"Wang","year":"2025","journal-title":"Microorganisms"},{"issue":"1","key":"10.1016\/j.fsi.2026.111744_bib37","doi-asserted-by":"crossref","DOI":"10.1186\/s13567-025-01496-x","article-title":"Generation of an artificially attenuated fowl adenovirus 4 viral vector using the reverse genetics system based on full-length infectious clone","volume":"56","author":"Tang","year":"2025","journal-title":"Vet. Res."},{"issue":"13","key":"10.1016\/j.fsi.2026.111744_bib38","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.00603-18","article-title":"The S gene is necessary but not sufficient for the virulence of porcine epidemic Diarrhea virus novel variant strain BJ2011C","volume":"92","author":"Wang","year":"2018","journal-title":"J. Virol."},{"issue":"5","key":"10.1016\/j.fsi.2026.111744_bib39","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1007\/s11427-020-1657-9","article-title":"A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs","volume":"63","author":"Chen","year":"2020","journal-title":"Sci. China Life Sci."},{"issue":"8","key":"10.1016\/j.fsi.2026.111744_bib40","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1016\/j.vaccine.2018.01.017","article-title":"Recombinant live attenuated avian coronavirus vaccines with deletions in the accessory genes 3ab and\/or 5ab protect against infectious bronchitis in chickens","volume":"36","author":"van Beurden","year":"2018","journal-title":"Vaccine"},{"issue":"3","key":"10.1016\/j.fsi.2026.111744_bib41","article-title":"An attenuated zika virus encoding non-glycosylated envelope (E) and non-structural protein 1 (NS1) confers complete protection against lethal challenge in a mouse model","volume":"7","author":"Annamalai","year":"2019","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.fsi.2026.111744_bib42","article-title":"SARS-CoV-2 nonstructural protein 3 remodels the phosphorylation of target proteins via protein-protein interactions","author":"Yang","year":"2025","journal-title":"bioRxiv"},{"key":"10.1016\/j.fsi.2026.111744_bib43","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2019.108395","article-title":"Identification of three site mutations in nonstructural protein 1beta, glycoprotein 3 and glycoprotein 5 that correlate with increased interferon alpha resistance of porcine reproductive and respiratory syndrome virus","volume":"236","author":"Su","year":"2019","journal-title":"Vet. Microbiol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111744_bib44","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1080\/22221751.2019.1679010","article-title":"The Nsp12-coding region of type 2 PRRSV is required for viral subgenomic mRNA synthesis","volume":"8","author":"Wang","year":"2019","journal-title":"Emerg. Microb. Infect."},{"issue":"12","key":"10.1016\/j.fsi.2026.111744_bib45","doi-asserted-by":"crossref","first-page":"3277","DOI":"10.1021\/acsinfecdis.1c00441","article-title":"In Vitro and In Vivo stability of P884T, a mutation that relocalizes Dengue virus 2 non-structural protein 5","volume":"7","author":"Cheng","year":"2021","journal-title":"ACS Infect. Dis."},{"issue":"Pt 6","key":"10.1016\/j.fsi.2026.111744_bib46","doi-asserted-by":"crossref","first-page":"1373","DOI":"10.1099\/vir.0.020818-0","article-title":"How RNA viruses maintain their genome integrity","volume":"91","author":"Barr","year":"2010","journal-title":"J. Gen. Virol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111744_bib47","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1128\/jvi.64.1.239-246.1990","article-title":"Editing of the Sendai virus P\/C mRNA by G insertion occurs during mRNA synthesis via a virus-encoded activity","volume":"64","author":"Vidal","year":"1990","journal-title":"J. Virol."},{"issue":"12","key":"10.1016\/j.fsi.2026.111744_bib48","doi-asserted-by":"crossref","first-page":"7391","DOI":"10.1128\/jvi.69.12.7391-7401.1995","article-title":"Genesis of Sindbis virus by in vivo recombination of nonreplicative RNA precursors","volume":"69","author":"Raju","year":"1995","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2026.111744_bib49","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2019.01280","article-title":"Calicivirus RNA-Dependent RNA polymerases: Evolution, structure, protein dynamics, and function","volume":"10","author":"Smertina","year":"2019","journal-title":"Front. Microbiol."},{"issue":"8","key":"10.1016\/j.fsi.2026.111744_bib50","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1016\/j.tig.2024.04.003","article-title":"Positive-strand RNA virus genome replication organelles: structure, assembly, control","volume":"40","author":"den Boon","year":"2024","journal-title":"Trends Genet."},{"issue":"24","key":"10.1016\/j.fsi.2026.111744_bib51","doi-asserted-by":"crossref","first-page":"12492","DOI":"10.1128\/JVI.01495-10","article-title":"Nodavirus-induced membrane rearrangement in replication complex assembly requires replicase protein a, RNA templates, and polymerase activity","volume":"84","author":"Kopek","year":"2010","journal-title":"J. Virol."},{"issue":"12","key":"10.1016\/j.fsi.2026.111744_bib52","doi-asserted-by":"crossref","DOI":"10.3390\/v14122711","article-title":"Multifunctional protein A is the only viral protein required for nodavirus RNA replication crown Formation","volume":"14","author":"den Boon","year":"2022","journal-title":"Viruses"}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826006480?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826006480?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T03:29:19Z","timestamp":1792985359000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464826006480"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":52,"alternative-id":["S1050464826006480"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111744","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Construction of red-spotted grouper nervous necrosis virus (RGNNV) nonstructural protein B1\/B2 mutant strains and dynamics during infection","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111744","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"111744"}}