{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T03:36:06Z","timestamp":1792985766216,"version":"4.5.0"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2024MD064"],"award-info":[{"award-number":["ZR2024MD064"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004739","name":"Youth Innovation Promotion Association of the Chinese Academy of Sciences","doi-asserted-by":"publisher","award":["2022211"],"award-info":[{"award-number":["2022211"]}],"id":[{"id":"10.13039\/501100004739","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011482","name":"Yantai Science and Technology Bureau","doi-asserted-by":"publisher","award":["2024YD089"],"award-info":[{"award-number":["2024YD089"]}],"id":[{"id":"10.13039\/501100011482","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42176141"],"award-info":[{"award-number":["42176141"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42476121"],"award-info":[{"award-number":["42476121"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.fsi.2026.111758","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:48:50Z","timestamp":1790869730000},"page":"111758","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Involvement of LTC4 and NADPH oxidase 5 in extracellular trap formation in Ruditapes philippinarum"],"prefix":"10.1016","volume":"179","author":[{"given":"Xiaohan","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanru","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunyu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiqing","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9230-0478","authenticated-orcid":false,"given":"Dinglong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianmin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsi.2026.111758_bib1","doi-asserted-by":"crossref","first-page":"11890","DOI":"10.1007\/s11356-015-4397-7","article-title":"Ruditapes philippinarum and Ruditapes decussatus under Hg environmental contamination","volume":"22","author":"Velez","year":"2015","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"10.1016\/j.fsi.2026.111758_bib2","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2026.1803024","article-title":"Immune priming reshapes the microbiota and modulates pathogen dynamics in the Manila clam (Ruditapes philippinarum)","volume":"17","author":"Rodino-Janeiro","year":"2026","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.fsi.2026.111758_bib3","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2025.110950","article-title":"Role of a ficolin in the innate immune mechanisms of Penaeus vannamei against Vibrio parahaemolyticus","volume":"168","author":"Guzman","year":"2026","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111758_bib4","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2025.110570","article-title":"Effect of dissolved oxygen concentration changes on the ultrastructure of blood cells in Manila clam (Ruditapes philippinarum)","volume":"165","author":"Zhou","year":"2025","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111758_bib5","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2024.1359600","article-title":"ROS signaling in innate immunity via oxidative protein modifications","volume":"15","author":"Manoharan","year":"2024","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2026.111758_bib6","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.fsi.2022.01.024","article-title":"Extracellular traps involved in invertebrate immune mechanisms","volume":"121","author":"Cubillo-Mart\u00ednez","year":"2022","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111758_bib7","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2019.103585","article-title":"Extracellular traps (ETosis) can be activated through NADPH-dependent and -independent mechanisms in bivalve mollusks","volume":"106","author":"Romero","year":"2020","journal-title":"Dev. Comp. Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111758_bib8","doi-asserted-by":"crossref","first-page":"2990","DOI":"10.1038\/s41467-024-46807-6","article-title":"Extracellular DNA traps in a ctenophore demonstrate immune cell behaviors in a non-bilaterian","volume":"15","author":"Vandepas","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.fsi.2026.111758_bib9","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2020.103953","article-title":"Hemocyte extracellular traps of Manila clam Ruditapes philippinarum: production characteristics and antibacterial effects","volume":"116","author":"Han","year":"2021","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2026.111758_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2021.104094","article-title":"Mitochondria are essential for antibacterial extracellular trap formation mediated by zymosan in hemocytes of Ruditapes philippinarum","volume":"121","author":"Han","year":"2021","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2026.111758_bib11","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2023.114909","article-title":"In vitro study of deltamethrin-induced extracellular traps in hemocytes of Ruditapes philippinarum","volume":"256","author":"Han","year":"2023","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"10.1016\/j.fsi.2026.111758_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.ecoenv.2024.117145","article-title":"Nonylphenol displays immunotoxicity by triggering hemocyte extracellular traps in Manila clam via ROS burst, ERK pathway and glycolysis","volume":"285","author":"Lv","year":"2024","journal-title":"Ecotoxicol. Environ. Saf."},{"issue":"8","key":"10.1016\/j.fsi.2026.111758_bib13","doi-asserted-by":"crossref","DOI":"10.1096\/fj.202500238RRR","article-title":"NOX family: regulators of reactive oxygen Species balance in tumor cells","volume":"39","author":"Xiong","year":"2025","journal-title":"FASEB J."},{"issue":"2","key":"10.1016\/j.fsi.2026.111758_bib14","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1007\/s13105-023-00955-3","article-title":"Structure, regulation, and physiological functions of NADPH oxidase 5 (NOX5)","volume":"79","author":"Garc\u00eda","year":"2023","journal-title":"J. Physiol. Biochem."},{"issue":"5","key":"10.1016\/j.fsi.2026.111758_bib15","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1002\/1873-3468.14577","article-title":"Ca2+-binding-region-dependent cell surface localization of NADPH oxidase Nox5","volume":"597","author":"Miyano","year":"2023","journal-title":"FEBS Lett."},{"issue":"3","key":"10.1016\/j.fsi.2026.111758_bib16","article-title":"Evolution of the ferric reductase domain (FRD) superfamily: modularity, functional diversification, and signature motifs","volume":"8","author":"Zhang","year":"2013","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.fsi.2026.111758_bib17","doi-asserted-by":"crossref","first-page":"3994","DOI":"10.1038\/s41467-024-48467-y","article-title":"Structural basis of human NOX5 activation","volume":"15","author":"Cui","year":"2024","journal-title":"Nat. Commun."},{"key":"10.1016\/j.fsi.2026.111758_bib18","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.109261","article-title":"Grass carp Il-2 promotes neutrophil extracellular traps formation via inducing ROS production and autophagy in vitro","volume":"144","author":"Lv","year":"2024","journal-title":"Fish Shellfish Immunol."},{"issue":"21","key":"10.1016\/j.fsi.2026.111758_bib19","doi-asserted-by":"crossref","DOI":"10.3390\/ijms242116001","article-title":"Targeting NADPH oxidase and Integrin \u03b15\u03b21 to inhibit neutrophil extracellular traps-mediated metastasis in colorectal cancer","volume":"24","author":"Zhu","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fsi.2026.111758_bib20","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/j.fsi.2019.08.073","article-title":"Florfenicol alleviated lipopolysaccharide (LPS)-induced inflammatory responses in Ctenopharyngodon idella through inhibiting toll\/NF-\u03baB signaling pathways","volume":"94","author":"Li","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111758_bib21","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.fsi.2017.08.025","article-title":"Various cellular responses of different shrimp haemocyte subpopulations to lipopolysaccharide stimulation","volume":"69","author":"Xian","year":"2017","journal-title":"Fish Shellfish Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111758_bib22","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/s41030-021-00152-x","article-title":"In vitro effects of 5-Lipoxygenase pathway inhibition on rhinovirus-associated bronchial epithelial inflammation","volume":"7","author":"Spyridaki","year":"2021","journal-title":"Pulm Ther"},{"key":"10.1016\/j.fsi.2026.111758_bib23","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms10112","article-title":"Leukotriene C4 is the major trigger of stress-induced oxidative DNA damage","volume":"6","author":"Dvash","year":"2015","journal-title":"Nat. Commun."},{"issue":"18","key":"10.1016\/j.fsi.2026.111758_bib24","doi-asserted-by":"crossref","first-page":"1662","DOI":"10.1093\/eurheartj\/ehae205","article-title":"Myocardial reperfusion injury exacerbation due to ALDH2 deficiency is mediated by neutrophil extracellular traps and prevented by leukotriene C4 inhibition","volume":"45","author":"Yang","year":"2024","journal-title":"Eur. Heart J."},{"issue":"1","key":"10.1016\/j.fsi.2026.111758_bib25","doi-asserted-by":"crossref","first-page":"2806","DOI":"10.1038\/s41598-025-86787-1","article-title":"Biosynthetic pathway for leukotrienes is stimulated by lipopolysaccharide and cytokines in pig endometrial stromal cells","volume":"15","author":"Jana","year":"2025","journal-title":"Sci. Rep."},{"issue":"21","key":"10.1016\/j.fsi.2026.111758_bib26","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.aeb8034","article-title":"Deciphering the ancestral mechanisms of neural regeneration through single-cell analyses of jellyfish rhopalium repair","volume":"12","author":"Li","year":"2026","journal-title":"Sci. Adv."},{"key":"10.1016\/j.fsi.2026.111758_bib27","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.molimm.2017.09.002","article-title":"Microsomal glutathione transferase 2 modulates LTC4 synthesis and ROS production in Apostichopus japonicus","volume":"91","author":"Zhang","year":"2017","journal-title":"Mol. Immunol."},{"key":"10.1016\/j.fsi.2026.111758_bib28","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.fsi.2019.04.297","article-title":"Fish species-specific TRIM gene FTRCA1 negatively regulates interferon response through attenuating IRF7 transcription","volume":"90","author":"Wu","year":"2019","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111758_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2023.108570","article-title":"Matrix metalloproteinase 9 modulates immune response along with the formation of extracellular traps in flounder (Paralichthys olivaceus)","volume":"133","author":"Chi","year":"2023","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111758_bib30","article-title":"Hippo-warts-yorkie pathway is crucial for larval survival and shell development in Pacific abalone (Haliotis discus hannai)","volume":"6","author":"Xue","year":"2024","journal-title":"Comp Immunol Rep"},{"key":"10.1016\/j.fsi.2026.111758_bib31","article-title":"Vaccine adjuvants induce Formation of intraperitoneal extracellular traps in flounder (Paralichthys olivaceus)","volume":"12","author":"Li","year":"2022","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.fsi.2026.111758_bib32","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.fsi.2022.08.060","article-title":"Triclocarban evoked neutrophil extracellular trap formation in common carp (Cyprinus carpio L.) by modulating SIRT3-mediated ROS crosstalk with ERK1\/2\/p38 signaling","volume":"129","author":"Li","year":"2022","journal-title":"Fish Shellfish Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111758_bib33","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/0014-2999(83)90384-9","article-title":"Leukotriene C4 (LTC4) elicits a respiratory burst in peritoneal macrophages","volume":"91","author":"Hartung","year":"1983","journal-title":"Eur. J. Pharmacol."},{"issue":"3","key":"10.1016\/j.fsi.2026.111758_bib34","doi-asserted-by":"crossref","first-page":"270","DOI":"10.3347\/PHD.24046","article-title":"CysLT receptor-mediated NOX2 activation is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products","volume":"62","author":"Lee","year":"2024","journal-title":"Parasites Hosts Dis"},{"issue":"18","key":"10.1016\/j.fsi.2026.111758_bib35","doi-asserted-by":"crossref","first-page":"18583","DOI":"10.1074\/jbc.M310268200","article-title":"Mechanism of Ca2+ activation of the NADPH oxidase 5 (NOX5)","volume":"279","author":"B\u00e1nfi","year":"2004","journal-title":"J. Biol. Chem."},{"issue":"4","key":"10.1016\/j.fsi.2026.111758_bib36","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/j.freeradbiomed.2011.11.023","article-title":"NOX5: from basic biology to signaling and disease","volume":"52","author":"Bedard","year":"2012","journal-title":"Free Radic. Biol. Med."},{"issue":"26","key":"10.1016\/j.fsi.2026.111758_bib37","doi-asserted-by":"crossref","first-page":"6764","DOI":"10.1073\/pnas.1702293114","article-title":"Crystal structures and atomic model of NADPH oxidase","volume":"114","author":"Magnani","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.fsi.2026.111758_bib38","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsi.2024.109985","article-title":"Arginine alleviates LPS-induced leukocytes inflammation and apoptosis via adjusted NODs signaling","volume":"154","author":"Qian","year":"2024","journal-title":"Fish Shellfish Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111758_bib39","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1146\/annurev-pathmechdis-111523-023509","article-title":"Cysteinyl leukotrienes in allergic inflammation","volume":"20","author":"Lee","year":"2025","journal-title":"Annu. Rev. Pathol."},{"key":"10.1016\/j.fsi.2026.111758_bib40","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.domaniend.2014.05.001","article-title":"Pro- and anti-inflammatory mediators change leukotriene B4 and leukotriene C4 synthesis and secretion in an inflamed porcine endometrium","volume":"49","author":"Czarzasta","year":"2014","journal-title":"Domest. Anim. Endocrinol."},{"key":"10.1016\/j.fsi.2026.111758_bib41","doi-asserted-by":"crossref","DOI":"10.1016\/j.redox.2023.102867","article-title":"Lactate-upregulated NADPH-dependent NOX4 expression via HCAR1\/PI3K pathway contributes to ROS-induced osteoarthritis chondrocyte damage","volume":"67","author":"Huang","year":"2023","journal-title":"Redox Biol."},{"issue":"3","key":"10.1016\/j.fsi.2026.111758_bib42","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/s00281-008-0118-3","article-title":"Priming of the neutrophil NADPH oxidase activation: role of p47phox phosphorylation and NOX2 mobilization to the plasma membrane","volume":"30","author":"El-Benna","year":"2008","journal-title":"Semin. Immunopathol."},{"key":"10.1016\/j.fsi.2026.111758_bib43","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2024.740953","article-title":"NOX-derived ROS modulates the antibacterial immune responses in coelomocytes of Apostichopus japonicus","volume":"588","author":"Yang","year":"2024","journal-title":"Aquacult"},{"key":"10.1016\/j.fsi.2026.111758_bib44","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.fsi.2022.02.012","article-title":"AMPK-mediated glutaminolysis maintains coelomocytes redox homeostasis in Vibrio splendidus-challenged Apostichopus japonicus","volume":"122","author":"Zhou","year":"2022","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111758_bib45","doi-asserted-by":"crossref","first-page":"4627","DOI":"10.1038\/ncomms5627","article-title":"Invertebrate extracellular phagocyte traps show that chromatin is an ancient defence weapon","volume":"5","author":"Robb","year":"2014","journal-title":"Nat. Commun."},{"key":"10.1016\/j.fsi.2026.111758_bib46","first-page":"112","article-title":"High throughput measurement of extracellular DNA release and quantitative NET Formation in human neutrophils in vitro","author":"Sil","year":"2016","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.fsi.2026.111758_bib47","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2022.943554","article-title":"Targeting ETosis by miR-155 inhibition mitigates mixed granulocytic asthmatic lung inflammation","volume":"13","author":"Kim","year":"2022","journal-title":"Front. Immunol."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826006625?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826006625?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T03:31:49Z","timestamp":1792985509000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464826006625"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":47,"alternative-id":["S1050464826006625"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111758","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Involvement of LTC4 and NADPH oxidase 5 in extracellular trap formation in Ruditapes philippinarum","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111758","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"111758"}}