{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T09:42:55Z","timestamp":1792748575563,"version":"4.5.0"},"reference-count":95,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,1,1]],"date-time":"2027-01-01T00:00:00Z","timestamp":1798761600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["IH190100014"],"award-info":[{"award-number":["IH190100014"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fish & Shellfish Immunology"],"published-print":{"date-parts":[[2027,1]]},"DOI":"10.1016\/j.fsi.2026.111779","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T23:25:12Z","timestamp":1791933912000},"page":"111779","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["An investigation into the RNA interference pathways in lobsters reveals key differences in immune factors across life stages"],"prefix":"10.1016","volume":"180","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8231-5564","authenticated-orcid":false,"given":"Jihye","family":"Yoon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas M.","family":"Banks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1843-8954","authenticated-orcid":false,"given":"Susan","family":"Glendinning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quinn P.","family":"Fitzgibbon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomer","family":"Ventura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"6836","key":"10.1016\/j.fsi.2026.111779_bib1","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1038\/35078107","article-title":"Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells","volume":"411","author":"Elbashir","year":"2001","journal-title":"Nature"},{"issue":"4","key":"10.1016\/j.fsi.2026.111779_bib2","doi-asserted-by":"crossref","first-page":"620","DOI":"10.3390\/genes4040620","article-title":"Gene silencing in crustaceans: from basic research to biotechnologies","volume":"4","author":"Sagi","year":"2013","journal-title":"Genes"},{"issue":"12","key":"10.1016\/j.fsi.2026.111779_bib3","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1007\/s13205-021-03049-3","article-title":"Innate and adaptive resistance to RNAi: a major challenge and hurdle to the development of double stranded RNA-based pesticides","volume":"11","author":"Choudhary","year":"2021","journal-title":"3 Biotech"},{"issue":"4","key":"10.1016\/j.fsi.2026.111779_bib4","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.3390\/ijms19041079","article-title":"A systematic study of RNAi effects and dsRNA stability in Tribolium castaneum and Acyrthosiphon pisum, following injection and ingestion of analogous dsRNAs","volume":"19","author":"Cao","year":"2018","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fsi.2026.111779_bib5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ibmb.2016.07.007","article-title":"Variation in RNAi efficacy among insect species is attributable to dsRNA degradation in vivo","volume":"77","author":"Wang","year":"2016","journal-title":"Insect Biochem. Mol. Biol."},{"issue":"4","key":"10.1016\/j.fsi.2026.111779_bib6","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1007\/s10340-021-01335-w","article-title":"Knockout of SldsRNase1 and SldsRNase2 revealed their function in dsRNA degradation and contribution to RNAi efficiency in the tobacco cutworm, Spodoptera litura","volume":"94","author":"Peng","year":"2021","journal-title":"J. Pest. Sci."},{"issue":"7","key":"10.1016\/j.fsi.2026.111779_bib7","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1080\/15476286.2016.1191728","article-title":"Reduced stability and intracellular transport of dsRNA contribute to poor RNAi response in lepidopteran insects","volume":"13","author":"Shukla","year":"2016","journal-title":"RNA Biol."},{"issue":"2","key":"10.1016\/j.fsi.2026.111779_bib8","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.ibmb.2006.11.004","article-title":"Molecular characterization of a cDNA encoding extracellular dsRNase and its expression in the silkworm, Bombyx mori","volume":"37","author":"Arimatsu","year":"2007","journal-title":"Insect Biochem. Mol. Biol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111779_bib9","article-title":"Purification and properties of double-stranded RNA-degrading nuclease, dsRNase, from the digestive juice of the silkworm, Bombyx mori","volume":"76","author":"Arimatsu","year":"2007","journal-title":"J. Insect Biotechnol. Sericol."},{"key":"10.1016\/j.fsi.2026.111779_bib10","article-title":"The effects of dsRNases on RNA interference efficiency in insects and future research directions","author":"Liu","year":"2026","journal-title":"Pestic. Biochem. Physiol."},{"issue":"3","key":"10.1016\/j.fsi.2026.111779_bib11","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/imb.12222","article-title":"The involvement of clathrin\u2010mediated endocytosis and two Sid\u20101\u2010like transmembrane proteins in double\u2010stranded RNA uptake in the Colorado potato beetle midgut","volume":"25","author":"Cappelle","year":"2016","journal-title":"Insect Mol. Biol."},{"issue":"2","key":"10.1016\/j.fsi.2026.111779_bib12","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1016\/j.cell.2017.03.033","article-title":"Circulating immune cells mediate a systemic RNAi-based adaptive antiviral response in drosophila","volume":"169","author":"Tassetto","year":"2017","journal-title":"Cell"},{"issue":"20","key":"10.1016\/j.fsi.2026.111779_bib13","doi-asserted-by":"crossref","first-page":"14370","DOI":"10.1074\/jbc.M513868200","article-title":"Double-stranded RNA is internalized by scavenger receptor-mediated endocytosis in Drosophila S2 cells","volume":"281","author":"Ulvila","year":"2006","journal-title":"J. Biol. Chem."},{"issue":"10","key":"10.1016\/j.fsi.2026.111779_bib14","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0047431","article-title":"Dissecting systemic RNA interference in the red flour beetle Tribolium castaneum: parameters affecting the efficiency of RNAi","volume":"7","author":"Miller","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2026.111779_bib15","doi-asserted-by":"crossref","DOI":"10.1186\/s12915-026-02526-6","article-title":"Cellular uptake of extracellular dsRNA is tissue-dependent in insects","author":"Shi","year":"2026","journal-title":"BMC Biol."},{"issue":"11","key":"10.1016\/j.fsi.2026.111779_bib16","doi-asserted-by":"crossref","first-page":"6718","DOI":"10.1093\/nar\/gkae395","article-title":"Structural basis for double-stranded RNA recognition by SID1","volume":"52","author":"Wang","year":"2024","journal-title":"Nucleic Acids Res."},{"issue":"31","key":"10.1016\/j.fsi.2026.111779_bib17","doi-asserted-by":"crossref","first-page":"18904","DOI":"10.1074\/jbc.M115.658864","article-title":"Systemic RNA interference deficiency-1 (SID-1) extracellular domain selectively binds long double-stranded RNA and is required for RNA transport by SID-1","volume":"290","author":"Li","year":"2015","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fsi.2026.111779_bib18","doi-asserted-by":"crossref","first-page":"553","DOI":"10.3389\/fphys.2016.00553","article-title":"RNAi efficiency, systemic properties, and novel delivery methods for pest insect control: what we know so far","volume":"7","author":"Joga","year":"2016","journal-title":"Front. Physiol."},{"key":"10.1016\/j.fsi.2026.111779_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2021.737557","article-title":"Systemic gene silencing from oral uptake of dsRNA in Litopenaeus vannamei requires both clathrin-mediated endocytosis and LvSID-1","volume":"548","author":"Maruekawong","year":"2022","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2026.111779_bib20","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.jinsphys.2014.02.010","article-title":"Lipophorins can adhere to dsRNA, bacteria and fungi present in the hemolymph of the desert locust: a role as general scavenger for pathogens in the open body cavity","volume":"64","author":"Wynant","year":"2014","journal-title":"J. Insect Physiol."},{"issue":"8","key":"10.1016\/j.fsi.2026.111779_bib21","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1016\/j.molimm.2006.09.021","article-title":"A Toll receptor in shrimp","volume":"44","author":"Yang","year":"2007","journal-title":"Mol. Immunol."},{"issue":"8","key":"10.1016\/j.fsi.2026.111779_bib22","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/ncb1439","article-title":"The endocytic pathway mediates cell entry of dsRNA to induce RNAi silencing","volume":"8","author":"Saleh","year":"2006","journal-title":"Nat. Cell Biol."},{"issue":"26","key":"10.1016\/j.fsi.2026.111779_bib23","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2322927121","article-title":"StaufenC facilitates utilization of the ERAD pathway to transport dsRNA through the endoplasmic reticulum to the cytosol","volume":"121","author":"Koo","year":"2024","journal-title":"Proc. Natl. Acad. Sci."},{"issue":"11","key":"10.1016\/j.fsi.2026.111779_bib24","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.3390\/biom14111358","article-title":"RNA interference applied to crustacean aquaculture","volume":"14","author":"Fajardo","year":"2024","journal-title":"Biomolecules"},{"key":"10.1016\/j.fsi.2026.111779_bib25","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.aquaculture.2012.09.018","article-title":"Effect of photoperiod on the culture of early-stage phyllosoma and metamorphosis of spiny lobster (Sagmariasus verreauxi)","volume":"368","author":"Fitzgibbon","year":"2012","journal-title":"Aquaculture"},{"key":"10.1016\/j.fsi.2026.111779_bib26","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.cbpa.2017.02.003","article-title":"Temperature dependent growth, feeding, nutritional condition and aerobic metabolism of juvenile spiny lobster, Sagmariasus verreauxi","volume":"207","author":"Fitzgibbon","year":"2017","journal-title":"Comp. Biochem. Physiol. Mol. Integr. Physiol."},{"key":"10.1016\/j.fsi.2026.111779_bib27","article-title":"Transcriptomic and nutritional analysis of the hepatopancreas in cultured juvenile Panulirus ornatus throughout the moult cycle","volume":"39","author":"Lewis","year":"2024","journal-title":"Aquac. Rep."},{"issue":"10","key":"10.1016\/j.fsi.2026.111779_bib28","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.3390\/genes11101150","article-title":"Multi-tissue transcriptome analysis identifies key sexual development-related genes of the ornate spiny lobster (Panulirus ornatus)","volume":"11","author":"Ventura","year":"2020","journal-title":"Genes"},{"key":"10.1016\/j.fsi.2026.111779_bib29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12864-020-07063-2","article-title":"CrustyBase: an interactive online database for crustacean transcriptomes","volume":"21","author":"Hyde","year":"2020","journal-title":"BMC Genom."},{"issue":"1","key":"10.1016\/j.fsi.2026.111779_bib30","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1186\/s12864-019-5925-5","article-title":"Transcriptional profiling of spiny lobster metamorphosis reveals three new additions to the nuclear receptor superfamily","volume":"20","author":"Hyde","year":"2019","journal-title":"BMC Genom."},{"issue":"19","key":"10.1016\/j.fsi.2026.111779_bib31","doi-asserted-by":"crossref","DOI":"10.3390\/ijms231911752","article-title":"A tale of two lobsters\u2014transcriptomic analysis reveals a potential gap in the RNA interference pathway in the tropical rock lobster Panulirus ornatus","volume":"23","author":"Banks","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fsi.2026.111779_bib32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s42995-025-00336-3","article-title":"Life stage-dependent RNA interference in the tropical rock lobster Panulirus ornatus: mechanistic insights from RNA-seq and functional studies","author":"Banks","year":"2026","journal-title":"Mar. Life Sci. Technol."},{"issue":"18","key":"10.1016\/j.fsi.2026.111779_bib33","doi-asserted-by":"crossref","first-page":"6967","DOI":"10.3390\/ijms21186967","article-title":"Double-Stranded RNA binding proteins in serum contribute to systemic RNAi across phyla\u2014towards finding the missing link in achelata","volume":"21","author":"Banks","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.fsi.2026.111779_bib34","series-title":"RNA Interference in Achelatan Lobsters-Exploring and Understanding Mechanisms of Gene Silencing to Promote Aquaculture Biotechnology","author":"Banks","year":"2026"},{"key":"10.1016\/j.fsi.2026.111779_bib35","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.ibmb.2017.05.008","article-title":"A double-stranded RNA degrading enzyme reduces the efficiency of oral RNA interference in migratory locust","volume":"86","author":"Song","year":"2017","journal-title":"Insect Biochem. Mol. Biol."},{"key":"10.1016\/j.fsi.2026.111779_bib36","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.ibmb.2019.04.001","article-title":"Nuclease activity decreases the RNAi response in the sweetpotato weevil Cylas puncticollis","volume":"110","author":"Prentice","year":"2019","journal-title":"Insect Biochem. Mol. Biol."},{"issue":"10","key":"10.1016\/j.fsi.2026.111779_bib37","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0047233","article-title":"Tsetse salivary gland proteins 1 and 2 are high affinity nucleic acid binding proteins with residual nuclease activity","volume":"7","author":"Caljon","year":"2012","journal-title":"PLoS One"},{"issue":"1","key":"10.1016\/j.fsi.2026.111779_bib38","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-17134-2","article-title":"Comparative analysis of double-stranded RNA degradation and processing in insects","volume":"7","author":"Singh","year":"2017","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.fsi.2026.111779_bib39","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s10750-018-3682-0","article-title":"Guidelines for RNA-seq projects: applications and opportunities in non-model decapod crustacean species","volume":"825","author":"Nguyen","year":"2018","journal-title":"Hydrobiologia"},{"issue":"43","key":"10.1016\/j.fsi.2026.111779_bib40","doi-asserted-by":"crossref","first-page":"15545","DOI":"10.1073\/pnas.0506580102","article-title":"Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles","volume":"102","author":"Subramanian","year":"2005","journal-title":"Proc. Natl. Acad. Sci."},{"issue":"1","key":"10.1016\/j.fsi.2026.111779_bib41","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/s12026-013-8384-0","article-title":"C-reactive protein and the biology of disease","volume":"56","author":"Ansar","year":"2013","journal-title":"Immunol. Res."},{"key":"10.1016\/j.fsi.2026.111779_bib42","article-title":"Mannose-binding C-type lectin from Procambarus clarkii exhibited antimicrobial activity to mediate crayfish innate immunity","volume":"32","author":"Huang","year":"2023","journal-title":"Aquac. Rep."},{"issue":"9","key":"10.1016\/j.fsi.2026.111779_bib43","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1005179","article-title":"DYRK2 negatively regulates type I interferon induction by promoting TBK1 degradation via Ser527 phosphorylation","volume":"11","author":"An","year":"2015","journal-title":"PLoS Pathog."},{"issue":"4","key":"10.1016\/j.fsi.2026.111779_bib44","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0062555","article-title":"Bumblebee venom serine protease increases fungal insecticidal virulence by inducing insect melanization","volume":"8","author":"Kim","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.fsi.2026.111779_bib45","doi-asserted-by":"crossref","DOI":"10.1016\/j.dci.2022.104557","article-title":"Comparative study of five anti-lipopolysaccharide factor genes in Litopenaeus vannamei","volume":"139","author":"Yin","year":"2023","journal-title":"Dev. Comp. Immunol."},{"key":"10.1016\/j.fsi.2026.111779_bib46","article-title":"Proteomic analysis of the hemolymph after Metschnikowia bicuspidata infection in the Chinese mitten crab Eriocheir sinensis","volume":"12","author":"Jiang","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2026.111779_bib47","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/j.fsi.2022.10.056","article-title":"A novel c-type lysozyme from Litopenaeus vannamei exhibits potent antimicrobial activity","volume":"131","author":"Hu","year":"2022","journal-title":"Fish Shellfish Immunol."},{"key":"10.1016\/j.fsi.2026.111779_bib48","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.fsi.2020.01.055","article-title":"RNA-Seq analysis reveals that spring viraemia of carp virus induces a broad spectrum of PIM kinases in zebrafish kidney that promote viral entry","volume":"99","author":"Pereiro","year":"2020","journal-title":"Fish Shellfish Immunol."},{"issue":"10","key":"10.1016\/j.fsi.2026.111779_bib49","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1011740","article-title":"Serpin-1a and serpin-6 regulate the Toll pathway immune homeostasis by synergistically inhibiting the Sp\u00e4tzle-processing enzyme CLIP2 in silkworm, Bombyx mori","volume":"19","author":"Liu","year":"2023","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.fsi.2026.111779_bib50","doi-asserted-by":"crossref","first-page":"738","DOI":"10.3389\/fimmu.2020.00738","article-title":"Phagocytic integrins: activation and signaling","volume":"11","author":"Torres-Gomez","year":"2020","journal-title":"Front. Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111779_bib51","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/biology10010001","article-title":"C-type lectin CD209L\/L-SIGN and CD209\/DC-SIGN: cell adhesion molecules turned to pathogen recognition receptors","volume":"10","author":"Rahimi","year":"2020","journal-title":"Biology"},{"issue":"3","key":"10.1016\/j.fsi.2026.111779_bib52","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1111\/imm.13846","article-title":"hnRNPs in antiviral innate immunity","volume":"173","author":"Maceratessi","year":"2024","journal-title":"Immunology"},{"issue":"4","key":"10.1016\/j.fsi.2026.111779_bib53","doi-asserted-by":"crossref","first-page":"582","DOI":"10.3390\/v13040582","article-title":"The antiviral activities of poly-adp-ribose polymerases","volume":"13","author":"Malgras","year":"2021","journal-title":"Viruses"},{"issue":"6","key":"10.1016\/j.fsi.2026.111779_bib54","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1016\/j.fsi.2010.08.007","article-title":"Molecular cloning, characterization, expression and activity analysis of cathepsin L in Chinese mitten crab, Eriocheir sinensis","volume":"29","author":"Li","year":"2010","journal-title":"Fish Shellfish Immunol."},{"issue":"19","key":"10.1016\/j.fsi.2026.111779_bib55","doi-asserted-by":"crossref","first-page":"10222","DOI":"10.1128\/JVI.00691-11","article-title":"Functional interactions between polydnavirus and host cellular innexins","volume":"85","author":"Marziano","year":"2011","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2026.111779_bib68","doi-asserted-by":"crossref","DOI":"10.3389\/fphys.2022.850022","article-title":"Multiple dsRNases involved in exogenous dsRNA degradation of fall armyworm Spodoptera frugiperda","volume":"13","author":"Yao","year":"2022","journal-title":"Front. Physiol."},{"issue":"11","key":"10.1016\/j.fsi.2026.111779_bib69","doi-asserted-by":"crossref","first-page":"6182","DOI":"10.3390\/ijms23116182","article-title":"RNAi efficiency through dsRNA injection is enhanced by knockdown of dsRNA nucleases in the fall webworm, Hyphantria cunea (Lepidoptera: arctiidae)","volume":"23","author":"Zhang","year":"2022","journal-title":"Int. J. Mol. Sci."},{"issue":"4","key":"10.1016\/j.fsi.2026.111779_bib70","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1261\/rna.079507.122","article-title":"Endosomal escape of RNA therapeutics: how do we solve this rate-limiting problem?","volume":"29","author":"Dowdy","year":"2023","journal-title":"Rna"},{"issue":"3","key":"10.1016\/j.fsi.2026.111779_bib71","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.jinsphys.2009.10.004","article-title":"Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: a review","volume":"56","author":"Huvenne","year":"2010","journal-title":"J. Insect Physiol."},{"issue":"6","key":"10.1016\/j.fsi.2026.111779_bib72","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1242\/bio.20147336","article-title":"Functional anatomy and ion regulatory mechanisms of the antennal gland in a semi-terrestrial crab, Ocypode stimpsoni","volume":"3","author":"Tsai","year":"2014","journal-title":"Biology Open"},{"key":"10.1016\/j.fsi.2026.111779_bib73","doi-asserted-by":"crossref","DOI":"10.1016\/j.aquaculture.2020.735932","article-title":"Antennal gland of shrimp as an entry for WSSV infection","volume":"530","author":"Liu","year":"2021","journal-title":"Aquaculture"},{"issue":"6","key":"10.1016\/j.fsi.2026.111779_bib74","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0177064","article-title":"Biomolecular changes that occur in the antennal gland of the giant freshwater prawn (machrobrachium rosenbergii)","volume":"12","author":"Bose","year":"2017","journal-title":"PLoS One"},{"issue":"11","key":"10.1016\/j.fsi.2026.111779_bib75","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.3390\/insects13111001","article-title":"Multiple functions of Malpighian tubules in insects: a review","volume":"13","author":"Farina","year":"2022","journal-title":"Insects"},{"key":"10.1016\/j.fsi.2026.111779_bib76","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.3389\/fimmu.2020.01066","article-title":"Phagocytosis: our current understanding of a universal biological process","volume":"11","author":"Uribe-Querol","year":"2020","journal-title":"Front. Immunol."},{"key":"10.1016\/j.fsi.2026.111779_bib77","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2022.920303","article-title":"Ligand-bound integrin \u03b1v\u03b26 internalisation and trafficking","volume":"10","author":"Meecham","year":"2022","journal-title":"Front. Cell Dev. Biol."},{"issue":"5","key":"10.1016\/j.fsi.2026.111779_bib78","doi-asserted-by":"crossref","first-page":"3951","DOI":"10.1128\/JVI.73.5.3951-3959.1999","article-title":"Cellular entry of hantaviruses which cause hemorrhagic fever with renal syndrome is mediated by \u03b23 integrins","volume":"73","author":"Gavrilovskaya","year":"1999","journal-title":"J. Virol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111779_bib79","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01474-18","article-title":"\u03b1v\u03b23 integrin is required for efficient infection of epithelial cells with human adenovirus type 26","volume":"93","author":"Nesti\u0107","year":"2019","journal-title":"J. Virol."},{"issue":"6","key":"10.1016\/j.fsi.2026.111779_bib80","doi-asserted-by":"crossref","DOI":"10.1016\/j.jbc.2025.110227","article-title":"The LDL receptor-related protein 1 (LRP1) facilitates ACE2-mediated endocytosis of SARS-CoV2 spike protein-containing pseudovirions","volume":"301","author":"Wani","year":"2025","journal-title":"J. Biol. Chem."},{"issue":"1","key":"10.1016\/j.fsi.2026.111779_bib81","doi-asserted-by":"crossref","first-page":"4036","DOI":"10.1038\/s41467-025-59305-0","article-title":"Genome-wide CRISPR screening identifies LRP1 as an entry factor for SFTSV","volume":"16","author":"Xing","year":"2025","journal-title":"Nat. Commun."},{"issue":"19","key":"10.1016\/j.fsi.2026.111779_bib82","doi-asserted-by":"crossref","first-page":"10442","DOI":"10.1128\/JVI.78.19.10442-10448.2004","article-title":"Induction of antiviral immunity by double-stranded RNA in a marine invertebrate","volume":"78","author":"Robalino","year":"2004","journal-title":"J. Virol."},{"issue":"5","key":"10.1016\/j.fsi.2026.111779_bib83","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1016\/j.molcel.2012.07.014","article-title":"Uptake of extracellular double-stranded RNA by SID-2","volume":"47","author":"McEwan","year":"2012","journal-title":"Mol. Cell"},{"issue":"20","key":"10.1016\/j.fsi.2026.111779_bib84","doi-asserted-by":"crossref","first-page":"R873","DOI":"10.1016\/j.cub.2012.08.039","article-title":"RNA interference: systemic RNAi SIDes with endosomes","volume":"22","author":"Rocheleau","year":"2012","journal-title":"Curr. Biol."},{"key":"10.1016\/j.fsi.2026.111779_bib85","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.ibmb.2017.09.011","article-title":"Accumulation of dsRNA in endosomes contributes to inefficient RNA interference in the fall armyworm, Spodoptera frugiperda","volume":"90","author":"Yoon","year":"2017","journal-title":"Insect Biochem. Mol. Biol."},{"key":"10.1016\/j.fsi.2026.111779_bib86","doi-asserted-by":"crossref","DOI":"10.1016\/j.phrs.2023.106665","article-title":"Endosomal escape of nucleic acids from extracellular vesicles mediates functional therapeutic delivery","volume":"188","author":"Pham","year":"2023","journal-title":"Pharmacol. Res."},{"key":"10.1016\/j.fsi.2026.111779_bib87","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.dci.2017.05.009","article-title":"Shrimp humoral responses against pathogens: antimicrobial peptides and melanization","volume":"80","author":"Tassanakajon","year":"2018","journal-title":"Dev. Comp. Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111779_bib88","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1600-065X.2009.00789.x","article-title":"Mannose\u2010binding lectin and innate immunity","volume":"230","author":"Eddie","year":"2009","journal-title":"Immunol. Rev."},{"issue":"1","key":"10.1016\/j.fsi.2026.111779_bib89","first-page":"1001","article-title":"Lectin in innate immunity of Crustacea","volume":"1","author":"Denis","year":"2016","journal-title":"Aust. Biol."},{"issue":"6","key":"10.1016\/j.fsi.2026.111779_bib56","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1016\/j.fsi.2007.04.001","article-title":"Phenoloxidase activity in the hemolymph of the spiny lobster Panulirus argus","volume":"23","author":"Perdomo-Morales","year":"2007","journal-title":"Fish Shellfish Immunol."},{"issue":"1","key":"10.1016\/j.fsi.2026.111779_bib90","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1111\/j.0105-2896.2004.00116.x","article-title":"The prophenoloxidase\u2010activating system in invertebrates","volume":"198","author":"Cerenius","year":"2004","journal-title":"Immunol. Rev."},{"issue":"10","key":"10.1016\/j.fsi.2026.111779_bib91","doi-asserted-by":"crossref","first-page":"6301","DOI":"10.1074\/jbc.M806764200","article-title":"A novel protein acts as a negative regulator of prophenoloxidase activation and melanization in the freshwater crayfish Pacifastacus leniusculus","volume":"284","author":"S\u00f6derh\u00e4ll","year":"2009","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.fsi.2026.111779_bib92","article-title":"DsRNA as pathogen-associated molecular pattern in innate immunity and multiple functions of the RNAi machinery complicate the use of RNAi in pest control","volume":"5","author":"Feng","year":"2025","journal-title":"Frontiers in Insect Science"},{"issue":"6","key":"10.1016\/j.fsi.2026.111779_bib93","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.jinsphys.2013.03.013","article-title":"Transcriptional response of BmToll9-1 and RNAi machinery genes to exogenous dsRNA in the midgut of Bombyx mori","volume":"59","author":"Liu","year":"2013","journal-title":"J. Insect Physiol."},{"issue":"8","key":"10.1016\/j.fsi.2026.111779_bib94","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1016\/j.jinsphys.2012.05.016","article-title":"Bombyx mori DNA\/RNA non-specific nuclease: expression of isoforms in insect culture cells, subcellular localization and functional assays","volume":"58","author":"Liu","year":"2012","journal-title":"J. Insect Physiol."},{"key":"10.1016\/j.fsi.2026.111779_bib95","doi-asserted-by":"crossref","DOI":"10.1016\/j.jinsphys.2025.104860","article-title":"Transcriptional activation of BmToll9-2 to exogenous dsRNA in the larvae of Bombyx mori","author":"Feng","year":"2025","journal-title":"J. Insect Physiol."},{"issue":"10","key":"10.1016\/j.fsi.2026.111779_bib96","doi-asserted-by":"crossref","first-page":"2088","DOI":"10.3390\/v14102088","article-title":"The role of mosquito hemocytes in viral infections","volume":"14","author":"Cardoso-Jaime","year":"2022","journal-title":"Viruses"},{"issue":"1","key":"10.1016\/j.fsi.2026.111779_bib97","doi-asserted-by":"crossref","first-page":"R10","DOI":"10.1186\/gb-2008-9-1-r10","article-title":"Exploring systemic RNA interference in insects: a genome-wide survey for RNAi genes in Tribolium","volume":"9","author":"Tomoyasu","year":"2008","journal-title":"Genome Biol."},{"issue":"8","key":"10.1016\/j.fsi.2026.111779_bib63","doi-asserted-by":"crossref","first-page":"1864","DOI":"10.3390\/cells10081864","article-title":"TRIM22. A multitasking antiviral factor","volume":"10","author":"Pagani","year":"2021","journal-title":"Cells"},{"issue":"9","key":"10.1016\/j.fsi.2026.111779_bib64","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.00885-23","article-title":"PARP12 is required to repress the replication of a Mac1 mutant coronavirus in a cell-and tissue-specific manner","volume":"97","author":"Kerr","year":"2023","journal-title":"J. Virol."},{"key":"10.1016\/j.fsi.2026.111779_bib65","article-title":"Deltex E3 ubiquitin ligase 2 potentiates STING-mediated type I interferon response by K63-linked ubiquitination","author":"Liu","year":"2026","journal-title":"Cell Death Dis."},{"key":"10.1016\/j.fsi.2026.111779_bib98","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbpa.2025.111901","article-title":"Characterization of the IGFBP-rP cDNA and its associations with growth traits in Macrobrachium rosenbergii","volume":"307","author":"Ding","year":"2025","journal-title":"Comp. Biochem. Physiol. Mol. Integr. Physiol."},{"issue":"7","key":"10.1016\/j.fsi.2026.111779_bib99","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1111\/j.1600-0706.2011.19725.x","article-title":"Impact of life stage specific immune priming on invertebrate disease dynamics","volume":"121","author":"Tate","year":"2012","journal-title":"Oikos"},{"issue":"1","key":"10.1016\/j.fsi.2026.111779_bib100","doi-asserted-by":"crossref","first-page":"6723","DOI":"10.1038\/s41598-020-63402-z","article-title":"Immune priming with inactive dengue virus during the larval stage of Aedes aegypti protects against the infection in adult mosquitoes","volume":"10","author":"Vargas","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.fsi.2026.111779_bib101","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.dci.2017.06.001","article-title":"Ontogenetic changes in the expression of immune related genes in response to immunostimulants and resistance against white spot syndrome virus in Litopenaeus vannamei","volume":"76","author":"Rajkumar","year":"2017","journal-title":"Dev. Comp. Immunol."},{"issue":"8","key":"10.1016\/j.fsi.2026.111779_bib102","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1071\/MF01186","article-title":"Distribution and transport pathways of Panulirus ornatus (Fabricius, 1776) and Panulirus spp. larvae in the Coral Sea, Australia","volume":"52","author":"Dennis","year":"2002","journal-title":"Mar. Freshw. Res."},{"issue":"8","key":"10.1016\/j.fsi.2026.111779_bib103","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1071\/MF97184","article-title":"Habitat use and growth of juvenile ornate rock lobsters, Panulirus ornatus (Fabricius, 1798), in Torres Strait, Australia","volume":"48","author":"Dennis","year":"1998","journal-title":"Mar. Freshw. Res."}],"container-title":["Fish & Shellfish Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826006832?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1050464826006832?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T09:15:30Z","timestamp":1792746930000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1050464826006832"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,1]]},"references-count":95,"alternative-id":["S1050464826006832"],"URL":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111779","relation":{},"ISSN":["1050-4648"],"issn-type":[{"value":"1050-4648","type":"print"}],"subject":[],"published":{"date-parts":[[2027,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An investigation into the RNA interference pathways in lobsters reveals key differences in immune factors across life stages","name":"articletitle","label":"Article Title"},{"value":"Fish & Shellfish Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsi.2026.111779","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"111779"}}