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Chem."},{"key":"10.1016\/j.fsigen.2025.103343_bib77","doi-asserted-by":"crossref","DOI":"10.1074\/mcp.M110.000513","article-title":"Novel oxidative modifications in redox-active cysteine residues","volume":"10","author":"Jeong","year":"2011","journal-title":"Mol. Cell. Proteom."},{"key":"10.1016\/j.fsigen.2025.103343_bib78","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1101\/gr.9.8.677","article-title":"dbSNP-Database for single nucleotide polymorphisms and other classes of minor genetic variation","volume":"9","author":"Sherry","year":"1999","journal-title":"Genome Res."},{"key":"10.1016\/j.fsigen.2025.103343_bib79","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1021\/pr0605419","article-title":"Comprehensive proteomic analysis of human cervical-vaginal fluid","volume":"6","author":"Dasari","year":"2007","journal-title":"J. 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