{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T14:27:12Z","timestamp":1793975232489,"version":"4.8.0"},"reference-count":23,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2027,2,1]],"date-time":"2027-02-01T00:00:00Z","timestamp":1801440000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:00Z","timestamp":1789171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","fsigenetics.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Forensic Science International: Genetics"],"published-print":{"date-parts":[[2027,2]]},"DOI":"10.1016\/j.fsigen.2026.103621","type":"journal-article","created":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T06:04:34Z","timestamp":1789279474000},"page":"103621","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Hyperplex PCR for forensic mRNA-based cell typing"],"prefix":"10.1016","volume":"87","author":[{"given":"Maja","family":"Sidstedt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margreet","family":"van den Berge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Titia","family":"Sijen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0124-6718","authenticated-orcid":false,"given":"Johannes","family":"Hedman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.fsigen.2026.103621_bib1","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.fsigen.2014.11.015","article-title":"Molecular approaches for forensic cell type identification: On mRNA, miRNA, DNA methylation and microbial markers","volume":"18","author":"Sijen","year":"2015","journal-title":"Forensic Sci. Int. Genet."},{"key":"10.1016\/j.fsigen.2026.103621_bib2","doi-asserted-by":"crossref","first-page":"545","DOI":"10.5483\/BMBRep.2012.45.10.206","article-title":"Body fluid identification in forensics","volume":"45","author":"An","year":"2012","journal-title":"BMB Rep."},{"key":"10.1016\/j.fsigen.2026.103621_bib3","doi-asserted-by":"crossref","DOI":"10.3390\/genes12111728","article-title":"On the identification of body fluids and tissues: a crucial link in the investigation and solution of crime","volume":"12","author":"Sijen","year":"2021","journal-title":"Genes. (Basel)"},{"key":"10.1016\/j.fsigen.2026.103621_bib4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.forsciint.2005.02.020","article-title":"Multiplex mRNA profiling for the identification of body fluids","volume":"152","author":"Juusola","year":"2005","journal-title":"Forensic Sci. Int."},{"key":"10.1016\/j.fsigen.2026.103621_bib5","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.fsigen.2015.10.011","article-title":"Advancing forensic RNA typing: On non-target secretions, a nasal mucosa marker, a differential co-extraction protocol and the sensitivity of DNA and RNA profiling","volume":"20","author":"van den Berge","year":"2016","journal-title":"Forensic Sci. Int. Genet."},{"key":"10.1016\/j.fsigen.2026.103621_bib6","article-title":"DNA methylation-based forensic framework for age prediction and body fluid identification using nanopore sequencing","volume":"81","author":"Beentjes","year":"2025","journal-title":"Forensic Sci. Int. Genet."},{"key":"10.1016\/j.fsigen.2026.103621_bib7","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.fsigen.2017.05.009","article-title":"Body fluid prediction from microbial patterns for forensic application","volume":"30","author":"Hanssen","year":"2017","journal-title":"Forensic Sci. Int. Genet."},{"key":"10.1016\/j.fsigen.2026.103621_bib8","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.fsigen.2018.07.012","article-title":"Optimizing body fluid recognition from microbial taxonomic profiles","volume":"37","author":"Hanssen","year":"2018","journal-title":"Forensic Sci. Int. Genet."},{"key":"10.1016\/j.fsigen.2026.103621_bib9","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1520\/JFS15560J","article-title":"Evaluation of mRNA markers for the identification of menstrual blood","volume":"47","author":"Bauer","year":"2002","journal-title":"J. Forensic Sci."},{"key":"10.1016\/j.fsigen.2026.103621_bib10","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsigen.2024.103125","article-title":"Comprehensive body fluid identification and contributor assignment by combining targeted sequencing of mRNA and coding region SNPs","volume":"73","author":"Neis","year":"2024","journal-title":"Forensic Sci. Int. Genet."},{"key":"10.1016\/j.fsigen.2026.103621_bib11","series-title":"US Patent Application 20230151407 A1","article-title":"A method for multiplexed detection of a plurality of target biomolecules","author":"Naseem","year":"2021"},{"key":"10.1016\/j.fsigen.2026.103621_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.watres.2025.123154","article-title":"Hyperplex PCR enables highly multiplexed analysis of point mutations in wastewater: long-term SARS-CoV-2 variant surveillance in Sweden as a case study","volume":"274","author":"Soares","year":"2025","journal-title":"Water Res."},{"key":"10.1016\/j.fsigen.2026.103621_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2022.160023","article-title":"Long-term SARS-CoV-2 surveillance in the wastewater of Stockholm: what lessons can be learned from the Swedish perspective?","volume":"858","author":"Perez-Zabaleta","year":"2023","journal-title":"Sci. Total. Environ."},{"key":"10.1016\/j.fsigen.2026.103621_bib14","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/978-1-0716-4767-7_16","article-title":"20-Plex gene expression analysis using hyperplex PCR (hpPCR) toward the development of salt-tolerant wheat","volume":"2969","author":"Viitanen","year":"2026","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.fsigen.2026.103621_bib15","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1126\/science.7522346","article-title":"Padlock probes: circularizing oligonucleotides for localized DNA detection","volume":"265","author":"Nilsson","year":"1994","journal-title":"Science"},{"key":"10.1016\/j.fsigen.2026.103621_bib16","doi-asserted-by":"crossref","first-page":"3324","DOI":"10.1039\/c3cs60439j","article-title":"Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine","volume":"43","author":"Ali","year":"2014","journal-title":"Chem. Soc. Rev."},{"key":"10.1016\/j.fsigen.2026.103621_bib17","series-title":"Proceedings of the 30th congress of the International Society for Forensic Genetics proceedings","article-title":"The use of rectal mucosa mRNA markers in forensic casework","author":"van den Berge","year":"2025"},{"key":"10.1016\/j.fsigen.2026.103621_bib18","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.fsigen.2009.11.007","article-title":"A method for DNA and RNA co-extraction for use on forensic samples using the Promega DNA IQ system","volume":"5","author":"Bowden","year":"2011","journal-title":"Forensic Sci. Int. Genet."},{"key":"10.1016\/j.fsigen.2026.103621_bib19","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.fsigen.2011.09.007","article-title":"Evaluation of mRNA marker specificity for the identification of five human body fluids by capillary electrophoresis","volume":"6","author":"Richard","year":"2012","journal-title":"Forensic Sci. Int. Genet."},{"key":"10.1016\/j.fsigen.2026.103621_bib20","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.legalmed.2011.07.002","article-title":"Expression of statherin mRNA and protein in nasal and vaginal secretions","volume":"13","author":"Sakurada","year":"2011","journal-title":"Leg. Med. (Tokyo)"},{"key":"10.1016\/j.fsigen.2026.103621_bib21","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.fsigen.2018.01.001","article-title":"Predicting the origin of stains from next generation sequencing mRNA data","volume":"34","author":"D\u00f6rum","year":"2018","journal-title":"Forensic Sci. Int. Genet."},{"key":"10.1016\/j.fsigen.2026.103621_bib22","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.1002\/elps.201700241","article-title":"Extended specificity studies of mRNA assays used to infer human organ tissues and body fluids","volume":"38","author":"van den Berge","year":"2017","journal-title":"Electrophoresis"},{"key":"10.1016\/j.fsigen.2026.103621_bib23","doi-asserted-by":"crossref","DOI":"10.1016\/j.fsigen.2020.102455","article-title":"Calculating LRs for presence of body fluids from mRNA assay data in mixtures","volume":"52","author":"Ypma","year":"2021","journal-title":"Forensic Sci. Int. Genet."}],"container-title":["Forensic Science International: Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1872497326002024?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1872497326002024?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T13:56:15Z","timestamp":1793973375000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1872497326002024"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2027,2]]},"references-count":23,"alternative-id":["S1872497326002024"],"URL":"https:\/\/doi.org\/10.1016\/j.fsigen.2026.103621","relation":{},"ISSN":["1872-4973"],"issn-type":[{"value":"1872-4973","type":"print"}],"subject":[],"published":{"date-parts":[[2027,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Hyperplex PCR for forensic mRNA-based cell typing","name":"articletitle","label":"Article Title"},{"value":"Forensic Science International: Genetics","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.fsigen.2026.103621","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Author(s). Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"103621"}}