{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T13:54:42Z","timestamp":1764251682823,"version":"build-2065373602"},"reference-count":52,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,5,1]],"date-time":"2021-05-01T00:00:00Z","timestamp":1619827200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fungal Biology"],"published-print":{"date-parts":[[2021,5]]},"DOI":"10.1016\/j.funbio.2020.12.004","type":"journal-article","created":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T03:11:56Z","timestamp":1608693116000},"page":"378-388","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"title":["The serine peptidase inhibitor N-\u03c1-tosyl-l-phenylalanine chloromethyl ketone (TPCK) affects the cell biology of Candida haemulonii species complex"],"prefix":"10.1016","volume":"125","author":[{"given":"X.M.","family":"Souto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.S.","family":"Ramos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.S.C.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.H.","family":"Branquinha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.L.S.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.funbio.2020.12.004_bib1","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1111\/1567-1364.12092","article-title":"Phenotypical properties associated with virulence from clinical isolates belonging to the Candida parapsilosis complex","volume":"13","author":"Abi-chacra","year":"2013","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.funbio.2020.12.004_bib2","doi-asserted-by":"crossref","first-page":"561","DOI":"10.3201\/eid2203.151610","article-title":"Candida haemulonii complex species, Brazil, January 2010-March 2015","volume":"22","author":"Almeida","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.funbio.2020.12.004_bib3","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1128\/JCM.44.2.553-560.2006","article-title":"Rapid susceptibility testing of medically important zygomycetes by XTT assay","volume":"44","author":"Antachopoulos","year":"2006","journal-title":"J.\u00a0Clin. Microbiol."},{"key":"10.1016\/j.funbio.2020.12.004_bib4","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1186\/s12879-016-1704-y","article-title":"Factors related to outcome of bloodstream infections due to Candida parapsilosis complex","volume":"16","author":"Barchiesi","year":"2016","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.funbio.2020.12.004_bib5","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.micpath.2017.10.040","article-title":"The natural compound magnolol affects growth, biofilm formation, and ultrastructure of oral Candida isolates","volume":"113","author":"Behbehani","year":"2017","journal-title":"Microb. Pathog."},{"key":"10.1016\/j.funbio.2020.12.004_bib6","doi-asserted-by":"crossref","first-page":"195","DOI":"10.3201\/eid2302.161486","article-title":"Multidrug-resistant Candida haemulonii and C.\u00a0auris, Tel Aviv, Israel","volume":"23","author":"Ben-Ami","year":"2017","journal-title":"Emerg. Infect. Dis. J."},{"key":"10.1016\/j.funbio.2020.12.004_bib7","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1111\/mmi.12621","article-title":"Bovine pancreatic trypsin inhibitor is a new antifungal peptide that inhibits cellular magnesium uptake","volume":"92","author":"Bleackley","year":"2014","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.funbio.2020.12.004_bib8","doi-asserted-by":"crossref","first-page":"5385","DOI":"10.1128\/JB.183.18.5385-5394.2001","article-title":"Biofilm formation by the fungal pathogen Candida albicans: development, architecture, and drug resistance","volume":"183","author":"Chandra","year":"2001","journal-title":"J.\u00a0Bacteriol."},{"year":"2008","series-title":"Clinical and Laboratory Standards Institute - Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts: Third Informational Supplement M27-S3","key":"10.1016\/j.funbio.2020.12.004_bib52"},{"key":"10.1016\/j.funbio.2020.12.004_bib9","doi-asserted-by":"crossref","DOI":"10.1155\/2015\/239519","article-title":"Artemisia princeps inhibits biofilm formation and virulence-factor expression of antibiotic-resistant bacteria","volume":"2015","author":"Choi","year":"2015","journal-title":"BioMed Res. Int."},{"key":"10.1016\/j.funbio.2020.12.004_bib10","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/s40560-018-0342-4","article-title":"Epidemiology, clinical characteristics, resistance, and treatment of infections by Candida auris","volume":"6","author":"Cortegiani","year":"2018","journal-title":"J.\u00a0Intensive Care"},{"key":"10.1016\/j.funbio.2020.12.004_bib11","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1001\/jama.2016.16535","article-title":"Associations of antifungal treatments with prevention of fungal infection in critically ill patients without neutropenia","volume":"317","author":"Cortegiani","year":"2017","journal-title":"J.\u00a0Am. Med. Assoc."},{"key":"10.1016\/j.funbio.2020.12.004_bib12","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1111\/j.1469-0691.2011.03471.x","article-title":"A\u00a0case of Candida haemulonii osteitis: clinical features, biochemical characteristics, and antifungal resistance profile","volume":"17","author":"Crouzet","year":"2011","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.funbio.2020.12.004_bib13","doi-asserted-by":"crossref","first-page":"755","DOI":"10.2217\/fmb.12.48","article-title":"Candida species: new insights into biofilm formation","volume":"7","author":"Cu\u00e9llar-Cruz","year":"2012","journal-title":"Future Microbiol."},{"key":"10.1016\/j.funbio.2020.12.004_bib14","doi-asserted-by":"crossref","first-page":"928","DOI":"10.1016\/j.archoralbio.2014.05.007","article-title":"Effect of the secretory leucocyte proteinase inhibitor (SLPI) on Candida albicans biological processes: a therapeutic alternative?","volume":"59","author":"Curvelo","year":"2014","journal-title":"Arch. Oral Biol."},{"key":"10.1016\/j.funbio.2020.12.004_bib15","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a019729","article-title":"Fungal biofilms, drug resistance, and recurrent infection","volume":"4","author":"Desai","year":"2014","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"10.1016\/j.funbio.2020.12.004_bib16","doi-asserted-by":"crossref","first-page":"92","DOI":"10.3389\/fmicb.2017.00092","article-title":"Regulation of sterol biosynthesis in the human fungal pathogen Aspergillus fumigatus: opportunities for therapeutic development","volume":"8","author":"Dhingra","year":"2017","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.funbio.2020.12.004_bib17","doi-asserted-by":"crossref","DOI":"10.1002\/9780471729259.mc02b02s25","article-title":"Scanning electron microscopy","author":"Fischer","year":"2012","journal-title":"Curr. Protoc. Microbiol."},{"key":"10.1016\/j.funbio.2020.12.004_bib18","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A\u00a0simple method for the isolation and purification of total lipides from animal tissues","volume":"226","author":"Folch","year":"1957","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.funbio.2020.12.004_bib19","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1093\/mmy\/myz008","article-title":"The serine peptidase inhibitor TPCK induces several morphophysiological changes in the opportunistic fungal pathogen Candida parapsilosis sensu stricto","volume":"57","author":"Gandra","year":"2019","journal-title":"Med. Mycol."},{"key":"10.1016\/j.funbio.2020.12.004_bib20","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1111\/j.1469-0691.2008.02660.x","article-title":"Differential in\u00a0vitro activity of anidulafungin, caspofungin and micafungin against Candida parapsilosis isolates recovered from a burn unit","volume":"15","author":"Ghannoum","year":"2009","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.funbio.2020.12.004_bib21","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S1567-1356(03)00154-5","article-title":"Sugar-induced apoptosis in yeast cells","volume":"4","author":"Granot","year":"2003","journal-title":"FEMS Yeast Res."},{"key":"10.1016\/j.funbio.2020.12.004_bib22","doi-asserted-by":"crossref","first-page":"27827","DOI":"10.1074\/jbc.M109.027912","article-title":"N(alpha)-tosyl-L-phenylalanine chloromethyl ketone induces caspase-dependent apoptosis in transformed human B cell lines with transcriptional down-regulation of anti-apoptotic HS1-associated protein X-1","volume":"284","author":"Jitkaew","year":"2009","journal-title":"J.\u00a0Biol. Chem."},{"key":"10.1016\/j.funbio.2020.12.004_bib23","first-page":"297","article-title":"Catheter-related candidemia caused by Candida haemulonii in a patient in long-term hospital care","volume":"26","author":"Kim","year":"2011","journal-title":"J.\u00a0Kor. Med. Sci."},{"key":"10.1016\/j.funbio.2020.12.004_bib24","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.chroma.2003.10.128","article-title":"Simplified and rapid method for extraction of ergosterol from natural samples and detection with quantitative and semi-quantitative methods using thin-layer chromatography","volume":"1026","author":"Larsen","year":"2004","journal-title":"J.\u00a0Chromatogr. A"},{"key":"10.1016\/j.funbio.2020.12.004_bib25","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1080\/14656566.2018.1454428","article-title":"Protease inhibitor therapy for hepatitis C virus-infection","volume":"19","author":"Leuw","year":"2018","journal-title":"Expet Opin. Pharmacother."},{"issue":"3","key":"10.1016\/j.funbio.2020.12.004_bib51","first-page":"110","article-title":"Increasing prevalence of multidrug-resistant Candida haemulonii species complex among all yeast cultures collected by a reference laboratory over the past 11 years","volume":"6","author":"Lima","year":"2020","journal-title":"J.\u00a0Fungi (Basel)"},{"key":"10.1016\/j.funbio.2020.12.004_bib26","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.2174\/1389203717666160809155749","article-title":"Contribution of aspartic proteases in Candida virulence. Protease inhibitors against Candida infections","volume":"18","author":"Monika","year":"2017","journal-title":"Curr. Protein Pept. Sci."},{"key":"10.1016\/j.funbio.2020.12.004_bib27","doi-asserted-by":"crossref","first-page":"9147","DOI":"10.1038\/s41598-017-09644-w","article-title":"Fungal lifestyle reflected in serine protease repertoire","volume":"7","author":"Muszewska","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.funbio.2020.12.004_bib28","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.resmic.2010.02.004","article-title":"Transcriptional response to fluconazole and amphotericin B in Candida albicans biofilms","volume":"161","author":"Nailis","year":"2010","journal-title":"Res. Microbiol."},{"key":"10.1016\/j.funbio.2020.12.004_bib29","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/j.resmic.2006.07.003","article-title":"Secretory aspartyl peptidase activity from mycelia of the human fungal pathogen Fonsecaea pedrosoi: effect of HIV aspartyl proteolytic inhibitors","volume":"157","author":"Palmeira","year":"2006","journal-title":"Res. Microbiol."},{"key":"10.1016\/j.funbio.2020.12.004_bib30","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0003382","article-title":"Beneficial effects of HIV peptidase inhibitors on Fonsecaea pedrosoi: promising compounds to arrest key fungal biological processes and virulence","volume":"3","author":"Palmeira","year":"2008","journal-title":"PloS One"},{"key":"10.1016\/j.funbio.2020.12.004_bib31","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.mimet.2007.11.010","article-title":"Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates","volume":"72","author":"Peeters","year":"2008","journal-title":"J.\u00a0Microbiol. Methods"},{"key":"10.1016\/j.funbio.2020.12.004_bib32","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1046\/j.1198-743x.2001.00307.x","article-title":"Cytometric approach for a rapid evaluation of susceptibility of Candida strains to antifungals","volume":"7","author":"Pina-Vaz","year":"2001","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.funbio.2020.12.004_bib33","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1093\/mmy\/myw065","article-title":"Different classes of hydrolytic enzymes produced by multidrug-resistant yeasts comprising the Candida haemulonii complex","volume":"55","author":"Ramos","year":"2017","journal-title":"Med. Mycol."},{"key":"10.1016\/j.funbio.2020.12.004_bib34","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1093\/jac\/dku321","article-title":"Candida haemulonii complex: species identification and antifungal susceptibility profiles of clinical isolates from Brazil","volume":"70","author":"Ramos","year":"2015","journal-title":"J.\u00a0Antimicrob. Chemother."},{"key":"10.1016\/j.funbio.2020.12.004_bib35","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1093\/mmy\/myz139","article-title":"Surface, adhesiveness and virulence aspects of Candida haemulonii species complex","volume":"58","author":"Ramos","year":"2020","journal-title":"Med. Mycol."},{"key":"10.1016\/j.funbio.2020.12.004_bib36","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1093\/mmy\/myx005","article-title":"Planktonic growth and biofilm formation profiles in Candida haemulonii species complex","volume":"55","author":"Ramos","year":"2017","journal-title":"Med. Mycol."},{"key":"10.1016\/j.funbio.2020.12.004_bib37","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1007\/s10482-014-0135-2","article-title":"Lipopeptides from Bacillus strain AR2 inhibits biofilm formation by Candida albicans","volume":"105","author":"Rautela","year":"2014","journal-title":"Antonie Leeuwenhoek"},{"key":"10.1016\/j.funbio.2020.12.004_bib38","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1007\/s10482-011-9683-x","article-title":"Capsicum annuum L. trypsin inhibitor as a template scaffold for new drug development against pathogenic yeast","volume":"101","author":"Ribeiro","year":"2012","journal-title":"Antonie Leeuwenhoek"},{"key":"10.1016\/j.funbio.2020.12.004_bib39","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.micron.2009.01.003","article-title":"In situ TEM and SEM studies on the antimicrobial activity and prevention of Candida albicans biofilm by Cassia spectabilis extract","volume":"40","author":"Sangetha","year":"2009","journal-title":"Micron"},{"key":"10.1016\/j.funbio.2020.12.004_bib40","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.3389\/fmicb.2018.01351","article-title":"Candida infections and therapeutic strategies: mechanisms of action for traditional and alternative agents","volume":"9","author":"Santos","year":"2018","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.funbio.2020.12.004_bib41","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1111\/j.1574-695X.2005.00023.x","article-title":"Secretion of serine peptidase by a clinical strain of Candida albicans: influence of growth conditions and cleavage of human serum proteins and extracellular matrix components","volume":"46","author":"Santos","year":"2006","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"10.1016\/j.funbio.2020.12.004_bib42","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.riam.2013.09.014","article-title":"Highlights in pathogenic fungal biofilms","volume":"31","author":"Sardi","year":"2014","journal-title":"Rev. Iberoam. De. Micol."},{"author":"Sigma","key":"10.1016\/j.funbio.2020.12.004_bib43"},{"key":"10.1016\/j.funbio.2020.12.004_bib44","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1111\/j.1574-6976.2012.00340.x","article-title":"Human pathogens utilize host extracellular matrix proteins laminin and collagen for adhesion and invasion of the host","volume":"36","author":"Singh","year":"2012","journal-title":"FEMS Microbiol. Rev."},{"key":"10.1016\/j.funbio.2020.12.004_bib45","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/BF01103778","article-title":"Carbohydrate and lipid components of hyphae and conidia of human pathogen Fonsecaea pedrosoi","volume":"132","author":"Soares","year":"1995","journal-title":"Mycopathologia"},{"key":"10.1016\/j.funbio.2020.12.004_bib46","doi-asserted-by":"crossref","DOI":"10.1590\/0001-3765201920180735","article-title":"Chymotrypsin- and trypsin-like activities secreted by the multidrug-resistant yeasts forming the Candida haemulonii complex","volume":"91","author":"Souto","year":"2019","journal-title":"An. Acad. Bras. Cienc."},{"key":"10.1016\/j.funbio.2020.12.004_bib47","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/s12223-018-0651-y","article-title":"Identification of cell-associated and secreted serine-type peptidases in multidrug-resistant emergent pathogens belonging to the Candida haemulonii complex","volume":"64","author":"Souto","year":"2019","journal-title":"Folia Microbiol."},{"key":"10.1016\/j.funbio.2020.12.004_bib48","first-page":"1023","article-title":"Nosocomial fungal infections: epidemiology, infection control, and prevention","volume":"30","author":"Suleyman","year":"2016","journal-title":"Infect. Dis. Clin."},{"key":"10.1016\/j.funbio.2020.12.004_bib49","doi-asserted-by":"crossref","DOI":"10.2217\/fmb.13.101","article-title":"Mechanisms of Candida biofilm drug resistance","volume":"8","author":"Taff","year":"2013","journal-title":"Future Microbiol."},{"key":"10.1016\/j.funbio.2020.12.004_bib50","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0132701","article-title":"Synergistic activity of the plant defensin HsAFP1 and caspofungin against Candida albicans biofilms and planktonic cultures","volume":"10","author":"Vriens","year":"2015","journal-title":"PloS One"}],"container-title":["Fungal Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S187861462030194X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S187861462030194X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T06:18:46Z","timestamp":1761805126000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S187861462030194X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5]]},"references-count":52,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["S187861462030194X"],"URL":"https:\/\/doi.org\/10.1016\/j.funbio.2020.12.004","relation":{},"ISSN":["1878-6146"],"issn-type":[{"type":"print","value":"1878-6146"}],"subject":[],"published":{"date-parts":[[2021,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The serine peptidase inhibitor N-\u03c1-tosyl-l-phenylalanine chloromethyl ketone (TPCK) affects the cell biology of Candida haemulonii species complex","name":"articletitle","label":"Article Title"},{"value":"Fungal Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.funbio.2020.12.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 British Mycological Society. Published by Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}