{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:51:27Z","timestamp":1792021887499,"version":"4.4.0"},"reference-count":40,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T00:00:00Z","timestamp":1793491200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Fungal Biology"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1016\/j.funbio.2026.101868","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:29:18Z","timestamp":1790206158000},"page":"101868","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"title":["Restoration of infectivity-virulence and toxin biosynthesis in Botrytis cinerea B459 via upstream-targeted marker-free CRISPR\/Cas9 editing of the transcription factor Bcin04g03490"],"prefix":"10.1016","volume":"130","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9576-1205","authenticated-orcid":false,"given":"V\u00edctor","family":"Coca-Ruiz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Saborido-Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5974-1877","authenticated-orcid":false,"given":"Michele C.","family":"Malvestiti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3563-1550","authenticated-orcid":false,"given":"Jan A.L.","family":"van Kan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9873-0970","authenticated-orcid":false,"given":"Ernesto P.","family":"Benito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5329-9582","authenticated-orcid":false,"given":"Josefina","family":"Aleu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isidro G.","family":"Collado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.funbio.2026.101868_bib1","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2021.663870","article-title":"A major effect gene controlling development and pathogenicity in Botrytis cinerea identified through genetic analysis of natural mycelial non-pathogenic isolates","volume":"12","author":"Acosta Morel","year":"2021","journal-title":"Front. Plant Sci."},{"key":"10.1016\/j.funbio.2026.101868_bib2","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1111\/ppa.12967","article-title":"Physiological and population genetic analysis of Botrytis field isolates from vineyards in Castilla y Le\u00f3n, Spain","volume":"68","author":"Acosta Morel","year":"2019","journal-title":"Plant Pathol."},{"key":"10.1016\/j.funbio.2026.101868_bib3","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1046\/j.1365-3059.2000.00452.x","article-title":"Genetic diversity in Botrytis cinerea populations on vegetable crops in greenhouses in south-eastern Spain","volume":"49","author":"Alfonso","year":"2000","journal-title":"Plant Pathol."},{"key":"10.1016\/j.funbio.2026.101868_bib4","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0084223","article-title":"Assessing the effects of light on differentiation and virulence of the plant pathogen Botrytis cinerea: characterization of the white collar complex","volume":"8","author":"Canessa","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.funbio.2026.101868_bib5","doi-asserted-by":"crossref","first-page":"2945","DOI":"10.1534\/g3.117.043331","article-title":"Functional profiling of transcription factor genes in Neurospora crassa","volume":"7","author":"Carrillo","year":"2017","journal-title":"G3: Genes, Genomes, Genet."},{"key":"10.1016\/j.funbio.2026.101868_bib6","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.1093\/plphys\/kiac037","article-title":"An update on precision genome editing by homology-directed repair in plants","volume":"188","author":"Chen","year":"2022","journal-title":"Plant Physiol."},{"key":"10.1016\/j.funbio.2026.101868_bib7","doi-asserted-by":"crossref","first-page":"932","DOI":"10.3390\/agriculture14060932","article-title":"Understanding the biology of the harmless isolate Botrytis cinerea B459: an approach to bio-targeted toxin identification","volume":"14","author":"Coca-Ruiz","year":"2024","journal-title":"Agriculture"},{"key":"10.1016\/j.funbio.2026.101868_bib8","article-title":"Loss of a major toxin gene cluster defines a metabolic schism and host-specific virulence in Botrytis pseudocinerea","volume":"19","author":"Coca-Ruiz","year":"2026","journal-title":"PLoS One"},{"key":"10.1016\/j.funbio.2026.101868_bib9","series-title":"The Biology of Botrytis","year":"1980"},{"key":"10.1016\/j.funbio.2026.101868_bib10","series-title":"Botrytis \u2013 the Fungus, the Pathogen and its Management in Agricultural Systems","first-page":"291","article-title":"Secondary metabolism in Botrytis cinerea: combining genomic and metabolomic approaches","author":"Collado","year":"2016"},{"key":"10.1016\/j.funbio.2026.101868_bib11","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1023\/A:1015209817830","article-title":"The putative role of botrydial and related metabolites in the infection mechanism of Botrytis cinerea","volume":"28","author":"Colmenares","year":"2002","journal-title":"J. Chem. Ecol."},{"key":"10.1016\/j.funbio.2026.101868_bib12","doi-asserted-by":"crossref","first-page":"553","DOI":"10.3390\/plants12030553","article-title":"From genes to molecules, secondary metabolism in Botrytis cinerea: new insights into anamorphic and teleomorphic stages","volume":"12","author":"da Silva Ripardo-Filho","year":"2023","journal-title":"Plants"},{"key":"10.1016\/j.funbio.2026.101868_bib13","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1111\/j.1364-3703.2010.00692.x","article-title":"The Botrytis cinerea phytotoxin botcinic acid requires two polyketide synthases for production and has a redundant role in virulence with botrydial","volume":"12","author":"Dalmais","year":"2011","journal-title":"Mol. Plant Pathol."},{"key":"10.1016\/j.funbio.2026.101868_bib14","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1111\/j.1364-3703.2011.00783.x","article-title":"The top 10 fungal pathogens in molecular plant pathology","volume":"13","author":"Dean","year":"2012","journal-title":"Mol. Plant Pathol."},{"key":"10.1016\/j.funbio.2026.101868_bib15","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/S0031-9422(01)00088-7","article-title":"Botrydial is produced in plant tissues infected by Botrytis cinerea","volume":"57","author":"Deighton","year":"2001","journal-title":"Phytochemistry"},{"key":"10.1016\/j.funbio.2026.101868_bib16","series-title":"Botrytis - the Fungus, the Pathogen and its Management in Agricultural Systems","first-page":"413","article-title":"Plant hosts of Botrytis spp","author":"Elad","year":"2015"},{"key":"10.1016\/j.funbio.2026.101868_bib17","series-title":"Botrytis - the Fungus, the Pathogen and its Management in Agricultural Systems","first-page":"1","article-title":"Botrytis, the good, the bad and the ugly","author":"Elad","year":"2015"},{"key":"10.1016\/j.funbio.2026.101868_bib18","article-title":"Dual-single-guide RNA strategy improves CRISPR-mediated homology-directed repair in Aspergillus","volume":"52","author":"Fu","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.funbio.2026.101868_bib19","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1094\/PHYTO-06-20-0211-IA","article-title":"The history of Botrytis taxonomy, the rise of phylogenetics, and implications for species recognition","volume":"111","author":"Garfinkel","year":"2021","journal-title":"Phytopathology"},{"key":"10.1016\/j.funbio.2026.101868_bib20","series-title":"Botrytis: Biology, Pathology and Control","first-page":"9","article-title":"Botrytis: biology, pathology and control","author":"Holz","year":"2007"},{"key":"10.1016\/j.funbio.2026.101868_bib21","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1111\/ppa.12143","article-title":"Genetic diversity of Botrytis in New Zealand vineyards and the significance of its seasonal and regional variation","volume":"63","author":"Johnston","year":"2014","journal-title":"Plant Pathol."},{"key":"10.1016\/j.funbio.2026.101868_bib22","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/mpp.12135","article-title":"Plant pathology 2.0","volume":"15","author":"Kang","year":"2014","journal-title":"Mol. Plant Pathol."},{"key":"10.1016\/j.funbio.2026.101868_bib23","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1016\/j.micres.2014.02.012","article-title":"Analyses of genetic and pathogenic variability among Botrytis cinerea isolates","volume":"169","author":"Kumari","year":"2014","journal-title":"Microbiol. Res."},{"key":"10.1016\/j.funbio.2026.101868_bib24","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1008326","article-title":"CRISPR\/Cas with ribonucleoprotein complexes and transiently selected telomere vectors allows highly efficient marker-free and multiple genome editing in Botrytis cinerea","volume":"16","author":"Leisen","year":"2020","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.funbio.2026.101868_bib25","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/0048-4059(85)90036-0","article-title":"Fungal nutrition and physiology","volume":"26","author":"Mansfield","year":"1985","journal-title":"Physiol. Plant Pathol."},{"key":"10.1016\/j.funbio.2026.101868_bib26","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1017\/S0953756202005981","article-title":"Genetic characterisation of Botrytis cinerea populations in Chile","volume":"106","author":"Mu\u00f1oz","year":"2002","journal-title":"Mycol. Res."},{"key":"10.1016\/j.funbio.2026.101868_bib27","article-title":"Molecular identification and characterization of Botrytis cinerea associated to the endemic flora of semi-desert climate in Chile","volume":"2","author":"Notte","year":"2021","journal-title":"Curr. Res. Microb. Sci."},{"key":"10.1016\/j.funbio.2026.101868_bib28","doi-asserted-by":"crossref","first-page":"7048","DOI":"10.1128\/AEM.01719-15","article-title":"Botrytis pseudocinerea is a significant pathogen of several crop plants but susceptible to displacement by fungicide-resistant B. cinerea strains","volume":"81","author":"Plesken","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.funbio.2026.101868_bib29","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.fgb.2016.10.003","article-title":"The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6 transcription factor BcBot6","volume":"96","author":"Porquier","year":"2016","journal-title":"Fungal Genet. Biol."},{"key":"10.1016\/j.funbio.2026.101868_bib30","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1002310","article-title":"A phenome-based functional analysis of transcription factors in the cereal head blight fungus, Fusarium graminearum","volume":"7","author":"Son","year":"2011","journal-title":"PLoS Pathog."},{"key":"10.1016\/j.funbio.2026.101868_bib31","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1093\/molbev\/msi020","article-title":"Molecular phylogeny of the plant pathogenic genus Botrytis and the evolution of host specificity","volume":"22","author":"Staats","year":"2005","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.funbio.2026.101868_bib32","doi-asserted-by":"crossref","first-page":"722","DOI":"10.1021\/np060071x","article-title":"Botcinins E and F and botcinolide from Botrytis cinerea and structural revision of botcinolides","volume":"69","author":"Tani","year":"2006","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.funbio.2026.101868_bib33","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.1021\/np0503855","article-title":"Botcinins A, B, C, and D, metabolites produced by Botrytis cinerea, and their antifungal activity against Magnaporthe grisea, a pathogen of rice blast disease","volume":"68","author":"Tani","year":"2005","journal-title":"J. Nat. Prod."},{"key":"10.1016\/j.funbio.2026.101868_bib34","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1111\/j.1364-3703.2011.00778.x","article-title":"BcAtf1, a global regulator, controls various differentiation processes and phytotoxin production in Botrytis cinerea","volume":"13","author":"Temme","year":"2012","journal-title":"Mol. Plant Pathol."},{"key":"10.1016\/j.funbio.2026.101868_bib35","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1094\/MPMI.1997.10.1.21","article-title":"Cloning and expression of the cutinase A gene of Botrytis cinerea","volume":"10","author":"van der Vlugt-Bergmans","year":"1997","journal-title":"Mol. Plant-Microbe Interact"},{"key":"10.1016\/j.funbio.2026.101868_bib36","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.tplants.2006.03.005","article-title":"Licensed to kill: the lifestyle of a necrotrophic plant pathogen","volume":"11","author":"van Kan","year":"2006","journal-title":"Trends Plant Sci."},{"key":"10.1016\/j.funbio.2026.101868_bib37","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1016\/j.tplants.2018.03.016","article-title":"Many shades of grey in Botrytis\u2013host plant interactions","volume":"23","author":"Veloso","year":"2018","journal-title":"Trends Plant Sci."},{"key":"10.1016\/j.funbio.2026.101868_bib38","series-title":"Botrytis \u2013 the Fungus, the Pathogen and its Management in Agricultural Systems","first-page":"91","article-title":"Diversity within and between species of Botrytis","author":"Walker","year":"2016"},{"key":"10.1016\/j.funbio.2026.101868_bib39","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1094\/PDIS-04-18-0688-PDN","article-title":"Botrytis pseudocinerea, a new pathogen causing gray mold on Brassica napus in China","volume":"103","author":"Xue","year":"2019","journal-title":"Plant Dis."},{"key":"10.1016\/j.funbio.2026.101868_bib40","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1016\/j.cell.2008.08.037","article-title":"Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double-strand break ends","volume":"134","author":"Zhu","year":"2008","journal-title":"Cell"}],"container-title":["Fungal Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1878614626001534?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1878614626001534?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:37:59Z","timestamp":1792021079000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1878614626001534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11]]},"references-count":40,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["S1878614626001534"],"URL":"https:\/\/doi.org\/10.1016\/j.funbio.2026.101868","relation":{},"ISSN":["1878-6146"],"issn-type":[{"value":"1878-6146","type":"print"}],"subject":[],"published":{"date-parts":[[2026,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Restoration of infectivity-virulence and toxin biosynthesis in Botrytis cinerea B459 via upstream-targeted marker-free CRISPR\/Cas9 editing of the transcription factor Bcin04g03490","name":"articletitle","label":"Article Title"},{"value":"Fungal Biology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.funbio.2026.101868","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Ltd on behalf of British Mycological Society.","name":"copyright","label":"Copyright"}],"article-number":"101868"}}