{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T13:20:38Z","timestamp":1760707238326},"reference-count":21,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2004,4,1]],"date-time":"2004-04-01T00:00:00Z","timestamp":1080777600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gene"],"published-print":{"date-parts":[[2004,4]]},"DOI":"10.1016\/j.gene.2004.02.006","type":"journal-article","created":{"date-parts":[[2004,3,30]],"date-time":"2004-03-30T05:20:32Z","timestamp":1080624032000},"page":"125-131","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Transactivator mutants with altered effector specificity allow selective regulation of two genes by tetracycline variants"],"prefix":"10.1016","volume":"331","author":[{"given":"Christel","family":"Krueger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina","family":"Danke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Hillen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Berens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.gene.2004.02.006_BIB1","doi-asserted-by":"crossref","first-page":"2723","DOI":"10.1093\/nar\/25.14.2723","article-title":"Tetracycline-controlled transcription in eukaryotes: novel transactivators with graded transactivation potential","volume":"25","author":"Baron","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.gene.2004.02.006_BIB2","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1073\/pnas.96.3.1013","article-title":"Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes","volume":"96","author":"Baron","year":"1999","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.gene.2004.02.006_BIB3","doi-asserted-by":"crossref","first-page":"3109","DOI":"10.1046\/j.1432-1033.2003.03694.x","article-title":"Gene regulation by tetracyclines. Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes","volume":"270","author":"Berens","year":"2003","journal-title":"Eur. J. Biochem."},{"key":"10.1016\/j.gene.2004.02.006_BIB4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1021\/bp000129c","article-title":"The impact of mammalian gene regulation concepts on functional genomic research, metabolic engineering, and advanced gene therapies","volume":"17","author":"Fussenegger","year":"2001","journal-title":"Biotechnol. Prog."},{"key":"10.1016\/j.gene.2004.02.006_BIB5","doi-asserted-by":"crossref","first-page":"5547","DOI":"10.1073\/pnas.89.12.5547","article-title":"Tight control of gene expression in mammalian cells by tetracycline-responsive promoters","volume":"89","author":"Gossen","year":"1992","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.gene.2004.02.006_BIB6","series-title":"Tetracyclines in Biology, Chemistry and Medicine","first-page":"139","article-title":"Tetracyclines in the control of gene expression in eukaryotes","author":"Gossen","year":"2002"},{"key":"10.1016\/j.gene.2004.02.006_BIB7","doi-asserted-by":"crossref","first-page":"261","DOI":"10.2174\/0929867013373642","article-title":"Inhibition of tumor cell invasiveness by chemically modified tetracyclines","volume":"8","author":"Gu","year":"2001","journal-title":"Curr. Med. Chem."},{"key":"10.1016\/j.gene.2004.02.006_BIB8","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1006\/jmbi.1997.1540","article-title":"Stepwise selection of TetR variants recognizing tet operator 4C with high affinity and specificity","volume":"276","author":"Helbl","year":"1998","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.gene.2004.02.006_BIB9","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1126\/science.8153629","article-title":"Structure of the Tet repressor\u2013tetracycline complex and regulation of antibiotic resistance","volume":"264","author":"Hinrichs","year":"1994","journal-title":"Science"},{"key":"10.1016\/j.gene.2004.02.006_BIB10","doi-asserted-by":"crossref","first-page":"3050","DOI":"10.1093\/nar\/gkg421","article-title":"Single-chain Tet transregulators","volume":"31","author":"Krueger","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.gene.2004.02.006_BIB11","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1002\/ijc.10168","article-title":"Inhibition of cell proliferation, invasion, tumor growth and metastasis by an oral non-antimicrobial tetracycline analog (COL-3) in a metastatic prostate cancer model","volume":"98","author":"Lokeshwar","year":"2002","journal-title":"Int. J. Cancer"},{"key":"10.1016\/j.gene.2004.02.006_BIB12","series-title":"Experiments in Molecular Genetics","author":"Miller","year":"1972"},{"key":"10.1016\/j.gene.2004.02.006_BIB13","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.gene.2004.02.006_BIB14","series-title":"Tetracyclines in Biology, Chemistry and Medicine","first-page":"3","article-title":"The chemistry and cellular biology of the tetracyclines","author":"Nelson","year":"2002"},{"key":"10.1016\/j.gene.2004.02.006_BIB15","doi-asserted-by":"crossref","first-page":"2755","DOI":"10.1128\/MCB.20.8.2755-2759.2000","article-title":"Translation of NRF mRNA is mediated by highly efficient internal ribosome entry","volume":"20","author":"Oumard","year":"2000","journal-title":"Mol. Cell. Biol."},{"key":"10.1016\/j.gene.2004.02.006_BIB16","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1093\/emboj\/17.2.535","article-title":"Determinants of protein\u2013protein recognition by four helix bundles: changing the dimerization specificity of Tet repressor","volume":"17","author":"Schnappinger","year":"1998","journal-title":"EMBO J."},{"key":"10.1016\/j.gene.2004.02.006_BIB17","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/S0022-2836(03)00427-3","article-title":"Teaching TetR to recognize a new inducer","volume":"329","author":"Scholz","year":"2003","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.gene.2004.02.006_BIB18","doi-asserted-by":"crossref","first-page":"2643","DOI":"10.1021\/ja00900a027","article-title":"6-Deoxytetracyclines: IV. Preparation, C-6 stereochemistry, and reactions","volume":"85","author":"Stephens","year":"1963","journal-title":"J. Am. Chem. Soc."},{"key":"10.1016\/j.gene.2004.02.006_BIB19","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1038\/sj.bjp.0704068","article-title":"Effects of chemically modified tetracyclines (CMTs) in sensitive, multidrug resistant and apoptosis resistant leukaemia cell lines","volume":"133","author":"Tolomeo","year":"2001","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.gene.2004.02.006_BIB20","doi-asserted-by":"crossref","first-page":"7963","DOI":"10.1073\/pnas.130192197","article-title":"Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity","volume":"97","author":"Urlinger","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.gene.2004.02.006_BIB21","doi-asserted-by":"crossref","first-page":"901","DOI":"10.1038\/nbt731","article-title":"Macrolide-based transgene control in mammalian cells and mice","volume":"20","author":"Weber","year":"2002","journal-title":"Nat. Biotechnol."}],"container-title":["Gene"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378111904000708?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378111904000708?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,2,14]],"date-time":"2019-02-14T04:53:42Z","timestamp":1550120022000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378111904000708"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4]]},"references-count":21,"alternative-id":["S0378111904000708"],"URL":"https:\/\/doi.org\/10.1016\/j.gene.2004.02.006","relation":{},"ISSN":["0378-1119"],"issn-type":[{"value":"0378-1119","type":"print"}],"subject":[],"published":{"date-parts":[[2004,4]]}}}