{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T17:59:52Z","timestamp":1775152792276,"version":"3.50.1"},"reference-count":16,"publisher":"Elsevier BV","issue":"1-2","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gene"],"published-print":{"date-parts":[[2008,9]]},"DOI":"10.1016\/j.gene.2008.06.021","type":"journal-article","created":{"date-parts":[[2008,6,23]],"date-time":"2008-06-23T16:22:34Z","timestamp":1214238154000},"page":"95-98","source":"Crossref","is-referenced-by-count":5,"title":["Vectors for enhanced gene expression and protein purification in Salmonella"],"prefix":"10.1016","volume":"421","author":[{"given":"Mohamed N.","family":"Seleem","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed","family":"Ali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen M.","family":"Boyle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nammalwar","family":"Sriranganathan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.gene.2008.06.021_bib1","doi-asserted-by":"crossref","first-page":"14652","DOI":"10.1073\/pnas.95.25.14652","article-title":"Upstream A-tracts increase bacterial promoter activity through interactions with the RNA polymerase alpha subunit","volume":"95","author":"Aiyar","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.gene.2008.06.021_bib2","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/0378-1119(83)90222-6","article-title":"Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli","volume":"25","author":"Amann","year":"1983","journal-title":"Gene"},{"key":"10.1016\/j.gene.2008.06.021_bib3","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1111\/j.1365-2958.1992.tb01351.x","article-title":"Isolation and molecular characterization of a novel broad-host-range plasmid from Bordetella bronchiseptica with sequence similarities to plasmids from gram-positive organisms","volume":"6","author":"Antoine","year":"1992","journal-title":"Mol. Microbiol."},{"key":"10.1016\/j.gene.2008.06.021_bib4","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1128\/CMR.5.3.328","article-title":"Oral immunization using live attenuated Salmonella spp. as carriers of foreign antigens","volume":"5","author":"Cardenas","year":"1992","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.gene.2008.06.021_bib5","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1128\/jb.173.3.1139-1144.1991","article-title":"Controlled expression of the transcriptional activator gene virG in Agrobacterium tumefaciens by using the Escherichia coli lac promoter","volume":"173","author":"Chen","year":"1991","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.gene.2008.06.021_bib6","doi-asserted-by":"crossref","first-page":"583","DOI":"10.3109\/08820138909112265","article-title":"Recombinant avirulent Salmonella vaccine strains with stable maintenance and high level expression of cloned genes in vivo","volume":"18","author":"Curtiss","year":"1989","journal-title":"Immunol. Invest."},{"key":"10.1016\/j.gene.2008.06.021_bib7","doi-asserted-by":"crossref","first-page":"9761","DOI":"10.1073\/pnas.95.17.9761","article-title":"Identification of an UP element consensus sequence for bacterial promoters","volume":"95","author":"Estrem","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.gene.2008.06.021_bib8","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.1128\/IAI.73.4.2005-2011.2005","article-title":"Antibiotic-free plasmid stabilization by operator-repressor titration for vaccine delivery by using live Salmonella enterica Serovar Typhimurium","volume":"73","author":"Garmory","year":"2005","journal-title":"Infect. Immun."},{"key":"10.1016\/j.gene.2008.06.021_bib9","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1128\/AEM.66.3.1216-1219.2000","article-title":"Development of a nonantibiotic dominant marker for positively selecting expression plasmids in multivalent Salmonella vaccines","volume":"66","author":"McNeill","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.gene.2008.06.021_bib10","doi-asserted-by":"crossref","first-page":"e78","DOI":"10.1093\/nar\/gnh076","article-title":"RNA stem-loop enhanced expression of previously non-expressible genes","volume":"32","author":"Paulus","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.gene.2008.06.021_bib11","doi-asserted-by":"crossref","first-page":"740","DOI":"10.2144\/04375BM05","article-title":"Improved expression vector for Brucella species","volume":"37","author":"Seleem","year":"2004","journal-title":"Biotechniques"},{"key":"10.1016\/j.gene.2008.06.021_bib12","doi-asserted-by":"crossref","first-page":"6833","DOI":"10.1128\/AEM.01446-06","article-title":"Establishment of a gene expression system in Ochrobactrum anthropi","volume":"72","author":"Seleem","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.gene.2008.06.021_bib13","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1007\/s00253-006-0555-7","article-title":"High-level heterologous gene expression in Ochrobactrum anthropi using an A-rich UP element","volume":"73","author":"Seleem","year":"2007","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.gene.2008.06.021_bib14","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1007\/s00253-007-0970-4","article-title":"Enhanced expression, detection and purification of recombinant proteins using RNA stem loop and tandem fusion tags","volume":"75","author":"Seleem","year":"2007","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"10.1016\/j.gene.2008.06.021_bib15","doi-asserted-by":"crossref","first-page":"3290","DOI":"10.1128\/IAI.68.6.3290-3296.2000","article-title":"Brucella abortus strain RB51 as a vector for heterologous protein expression and induction of specific Th1 type immune responses","volume":"68","author":"Vemulapalli","year":"2000","journal-title":"Infect. Immun."},{"key":"10.1016\/j.gene.2008.06.021_bib16","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0378-1119(99)00541-7","article-title":"Construction of an efficient expression system for Agrobacterium tumefaciens based on the coliphage T5 promoter","volume":"242","author":"Wang","year":"2000","journal-title":"Gene"}],"container-title":["Gene"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378111908002539?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378111908002539?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,5,12]],"date-time":"2019-05-12T08:36:49Z","timestamp":1557650209000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378111908002539"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,9]]},"references-count":16,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[2008,9]]}},"alternative-id":["S0378111908002539"],"URL":"https:\/\/doi.org\/10.1016\/j.gene.2008.06.021","relation":{},"ISSN":["0378-1119"],"issn-type":[{"value":"0378-1119","type":"print"}],"subject":[],"published":{"date-parts":[[2008,9]]}}}