{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T21:12:44Z","timestamp":1774559564062,"version":"3.50.1"},"reference-count":25,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81901553"],"award-info":[{"award-number":["81901553"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2021JJ30461"],"award-info":[{"award-number":["2021JJ30461"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Gene"],"published-print":{"date-parts":[[2023,12]]},"DOI":"10.1016\/j.gene.2023.147745","type":"journal-article","created":{"date-parts":[[2023,8,26]],"date-time":"2023-08-26T11:12:00Z","timestamp":1693048320000},"page":"147745","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"special_numbering":"C","title":["Novel biallelic ASTL variants are associated with polyspermy and female infertility: A successful live birth following ICSI treatment"],"prefix":"10.1016","volume":"887","author":[{"given":"Huixia","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianfang","family":"Che","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dehua","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Gong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangxiu","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ge","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6185-3590","authenticated-orcid":false,"given":"Can","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"issue":"7497","key":"10.1016\/j.gene.2023.147745_b0005","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1038\/nature13203","article-title":"Juno is the egg Izumo receptor and is essential for mammalian fertilization","volume":"508","author":"Bianchi","year":"2014","journal-title":"Nature"},{"key":"10.1016\/j.gene.2023.147745_b0010","doi-asserted-by":"crossref","unstructured":"Burkart, A.D., Xiong, B., Baibakov, B., Jimenez-Movilla, M., Dean, J., 2012. Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy. J. Cell Biol., 197, 37-44. cell biology 2012;197: 37-44 .","DOI":"10.1083\/jcb.201112094"},{"key":"10.1016\/j.gene.2023.147745_b0015","article-title":"Novel homozygous variations in PLCZ1 lead to poor or failed fertilization characterized by abnormal localization patterns of PLCzeta in sperm","author":"Dai","year":"2019","journal-title":"Clin. Genet."},{"issue":"11","key":"10.1016\/j.gene.2023.147745_b0020","first-page":"2201","article-title":"ZP1 mutations are associated with empty follicle syndrome: evidence for the existence of an intact oocyte and a zona pellucida in follicles up to the early antral stage. A case report","volume":"34","author":"Dai","year":"2019","journal-title":"Human Reproduction"},{"key":"10.1016\/j.gene.2023.147745_b0025","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1038\/s41436-018-0064-y","article-title":"ZP2 pathogenic variants cause in vitro fertilization failure and female infertility","volume":"21","author":"Dai","year":"2019","journal-title":"Genet. Med.: Off. J. Am. College Med. Genet."},{"issue":"3","key":"10.1016\/j.gene.2023.147745_b0030","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1016\/j.fertnstert.2018.11.013","article-title":"New biallelic mutations in WEE2: expanding the spectrum of mutations that cause fertilization failure or poor fertilization","volume":"111","author":"Dai","year":"2019","journal-title":"Fertility Sterility"},{"issue":"3","key":"10.1016\/j.gene.2023.147745_b0035","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1016\/j.ajhg.2021.02.004","article-title":"Homozygous pathogenic variants in ACTL9 cause fertilization failure and male infertility in humans and mice","volume":"108","author":"Dai","year":"2021","journal-title":"Am. J. Human Genet."},{"key":"10.1016\/j.gene.2023.147745_b0040","doi-asserted-by":"crossref","first-page":"e16501","DOI":"10.15252\/emmm.202216501","article-title":"IQCN disruption causes fertilization failure and male infertility due to manchette assembly defect","volume":"14","author":"Dai","year":"2022","journal-title":"EMBO Mol. Med."},{"issue":"3","key":"10.1016\/j.gene.2023.147745_b0045","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1002\/mrd.23320","article-title":"Mammalian egg coat modifications and the block to polyspermy","volume":"87","author":"Fahrenkamp","year":"2020","journal-title":"Mol. Reprod. Develop."},{"issue":"18","key":"10.1016\/j.gene.2023.147745_b0050","doi-asserted-by":"crossref","first-page":"13958","DOI":"10.1074\/jbc.M109.097436","article-title":"Proenzyme structure and activation of astacin metallopeptidase","volume":"285","author":"Guevara","year":"2010","journal-title":"J. Biol. Chem."},{"issue":"3","key":"10.1016\/j.gene.2023.147745_b0055","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1002\/mrd.23235","article-title":"Physiological polyspermy: Selection of a sperm nucleus for the development of diploid genomes in amphibians","volume":"87","author":"Iwao","year":"2020","journal-title":"Mol. Reprod. Dev"},{"issue":"7873","key":"10.1016\/j.gene.2023.147745_b0060","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"10.1016\/j.gene.2023.147745_b0065","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1093\/molehr\/gax040","article-title":"Intracellular activation of ovastacin mediates pre-fertilization hardening of the zona pellucida","volume":"23","author":"Korschgen","year":"2017","journal-title":"Mol. Hum. Reprod."},{"issue":"1","key":"10.1016\/j.gene.2023.147745_b0070","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1007\/s00439-021-02388-8","article-title":"ASTL is mutated in female infertility","volume":"141","author":"Maddirevula","year":"2022","journal-title":"Hum. Genet."},{"key":"10.1016\/j.gene.2023.147745_b0075","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1093\/biolre\/ioab105","article-title":"Sperm migration, selection, survival, and fertilizing ability in the mammalian oviductdagger","volume":"105","author":"Mahe","year":"2021","journal-title":"Biol. Reprod."},{"key":"10.1016\/j.gene.2023.147745_b0080","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1038\/s41598-018-19497-6","article-title":"Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice","volume":"8","author":"Nozawa","year":"2018","journal-title":"Sci. Rep."},{"issue":"1","key":"10.1016\/j.gene.2023.147745_b0085","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s10815-022-02670-2","article-title":"Mutations in PLCZ1 induce male infertility associated with polyspermy and fertilization failure","volume":"40","author":"Peng","year":"2023","journal-title":"J. Assist. Reprod. Genet."},{"issue":"25","key":"10.1016\/j.gene.2023.147745_b0090","doi-asserted-by":"crossref","first-page":"26627","DOI":"10.1074\/jbc.M401588200","article-title":"Identification and characterization of human and mouse ovastacin: a novel metalloproteinase similar to hatching enzymes from arthropods, birds, amphibians, and fish","volume":"279","author":"Quesada","year":"2004","journal-title":"J. Biol. Chem."},{"issue":"2","key":"10.1016\/j.gene.2023.147745_b0095","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.ydbio.2012.09.016","article-title":"An oocyte-specific astacin family protease, alveolin, is released from cortical granules to trigger egg envelope hardening during fertilization in medaka (Oryzias latipes)","volume":"372","author":"Shibata","year":"2012","journal-title":"Dev. Biol."},{"key":"10.1016\/j.gene.2023.147745_b0100","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1093\/humupd\/dmn025","article-title":"ART failure: oocyte contributions to unsuccessful fertilization","volume":"14","author":"Swain","year":"2008","journal-title":"Hum. Reprod. Update"},{"issue":"1 In Vitro Fert","key":"10.1016\/j.gene.2023.147745_b0105","first-page":"88","article-title":"Polyspermy in in vitro fertilization of human oocytes: frequency and possible causes","volume":"442","author":"Ven","year":"1985","journal-title":"Ann. N. Y. Acad. Sci."},{"issue":"4","key":"10.1016\/j.gene.2023.147745_b0110","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1002\/jemt.10346","article-title":"How does polyspermy happen in mammalian oocytes?","volume":"61","author":"Wang","year":"2003","journal-title":"Microsc. Res. Tech."},{"issue":"1","key":"10.1016\/j.gene.2023.147745_b0115","doi-asserted-by":"crossref","first-page":"e1006580","DOI":"10.1371\/journal.pgen.1006580","article-title":"A Unique Egg Cortical Granule Localization Motif Is Required for Ovastacin Sequestration to Prevent Premature ZP2 Cleavage and Ensure Female Fertility in Mice","volume":"13","author":"Xiong","year":"2017","journal-title":"PLoS Genet."},{"issue":"2","key":"10.1016\/j.gene.2023.147745_b0120","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S0022-2836(02)01102-6","article-title":"Activation mechanism of pro-astacin: role of the pro-peptide, tryptic and autoproteolytic cleavage and importance of precise amino-terminal processing","volume":"324","author":"Yiallouros","year":"2002","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.gene.2023.147745_b0125","doi-asserted-by":"crossref","unstructured":"Zeng, Y., Chen, B., Sun, Y., Yang, A., Wu, L., Li, B., Mu, J., Zhang, Z., Wang, W., Zhou, Z., et al., 2023. Bi-allelic variants in ASTL cause abnormal fertilization or oocyte maturation defects. Human Mol. Genet., 32, 2326-2334.","DOI":"10.1093\/hmg\/ddad070"}],"container-title":["Gene"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378111923005863?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378111923005863?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T17:07:55Z","timestamp":1759856875000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378111923005863"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12]]},"references-count":25,"alternative-id":["S0378111923005863"],"URL":"https:\/\/doi.org\/10.1016\/j.gene.2023.147745","relation":{},"ISSN":["0378-1119"],"issn-type":[{"value":"0378-1119","type":"print"}],"subject":[],"published":{"date-parts":[[2023,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Novel biallelic ASTL variants are associated with polyspermy and female infertility: A successful live birth following ICSI treatment","name":"articletitle","label":"Article Title"},{"value":"Gene","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.gene.2023.147745","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"147745"}}