{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T17:00:32Z","timestamp":1793466032360,"version":"4.5.0"},"reference-count":45,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100023983","name":"Babasaheb Bhimrao Ambedkar University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100023983","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100019473","name":"Ministry of Science and Technology, Pakistan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100019473","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001412","name":"Council of Scientific & Industrial Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Gene"],"published-print":{"date-parts":[[2025,2]]},"DOI":"10.1016\/j.gene.2024.149160","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T16:34:06Z","timestamp":1734021246000},"page":"149160","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["From ancestor to pathogen: Expansion and evolutionary adaptations of multidrug resistance causing MFS efflux pumps in mycobacteria"],"prefix":"10.1016","volume":"938","author":[{"given":"Garima","family":"Singh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuf","family":"Akhter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.gene.2024.149160_b0005","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2021.612675","article-title":"Evolution of drug-resistant mycobacterium tuberculosis strains and their adaptation to the human lung environment","volume":"12","author":"Allu\u00e9-Guardia","year":"2021","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.gene.2024.149160_b0010","doi-asserted-by":"crossref","first-page":"333","DOI":"10.2147\/IDR.S144446","article-title":"Diversity and evolution of drug resistance mechanisms in <em>Mycobacterium tuberculosis<\/em>","volume":"10","author":"Al-Saeedi","year":"2017","journal-title":"Infect. Drug Resist."},{"issue":"3","key":"10.1016\/j.gene.2024.149160_b0015","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. Biol."},{"issue":"1","key":"10.1016\/j.gene.2024.149160_b0020","doi-asserted-by":"crossref","first-page":"e20","DOI":"10.1016\/S2666-5247(22)00359-7","article-title":"WHO\u2019s Global Tuberculosis Report 2022","volume":"4","author":"Bagcchi","year":"2023","journal-title":"The Lancet Microbe"},{"issue":"5","key":"10.1016\/j.gene.2024.149160_b0025","doi-asserted-by":"crossref","first-page":"2643","DOI":"10.1128\/AAC.06003-11","article-title":"Efflux pumps of mycobacterium tuberculosis play a significant role in antituberculosis activity of potential drug candidates","volume":"56","author":"Balganesh","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"issue":"1","key":"10.1016\/j.gene.2024.149160_b0030","doi-asserted-by":"crossref","first-page":"21","DOI":"10.3201\/eid2001.130652","article-title":"Progenitor \u201c Mycobacterium canettii \u201d clone responsible for lymph node tuberculosis epidemic, Djibouti","volume":"20","author":"Blouin","year":"2014","journal-title":"Emerg. Infect. Dis."},{"issue":"1","key":"10.1016\/j.gene.2024.149160_b0035","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/imr.12264","article-title":"Co\u2010evolution of <scp>M<\/scp> ycobacterium tuberculosis and <scp>H<\/scp> omo sapiens","volume":"264","author":"Brites","year":"2015","journal-title":"Immunol. Rev."},{"key":"10.1016\/j.gene.2024.149160_b0040","doi-asserted-by":"crossref","first-page":"S26","DOI":"10.1016\/j.ijid.2022.03.011","article-title":"The WHO Global Tuberculosis 2021 Report \u2013 not so good news and turning the tide back to End TB","volume":"124","author":"Chakaya","year":"2022","journal-title":"Int. J. Infect. Dis."},{"issue":"1","key":"10.1016\/j.gene.2024.149160_b0045","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1186\/1471-2164-15-308","article-title":"Genome-wide Mycobacterium tuberculosis variation (GMTV) database: a new tool for integrating sequence variations and epidemiology","volume":"15","author":"Chernyaeva","year":"2014","journal-title":"BMC Genomics"},{"issue":"11","key":"10.1016\/j.gene.2024.149160_b0050","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1007\/BF03402035","article-title":"The multidrug transporters belonging to Major Facilitator Superfamily (MFS) in Mycobacterium tuberculosis","volume":"8","author":"De Rossi","year":"2002","journal-title":"Mol. Med."},{"issue":"W1","key":"10.1016\/j.gene.2024.149160_b0055","doi-asserted-by":"crossref","first-page":"W408","DOI":"10.1093\/nar\/gkv451","article-title":"CCTOP: a consensus constrained TOPology prediction web server","volume":"43","author":"Dobson","year":"2015","journal-title":"Nucleic Acids Res."},{"issue":"9","key":"10.1016\/j.gene.2024.149160_b0060","doi-asserted-by":"crossref","first-page":"5289","DOI":"10.1021\/acs.chemrev.0c00983","article-title":"Structures and general transport mechanisms by the Major Facilitator Superfamily (MFS)","volume":"121","author":"Drew","year":"2021","journal-title":"Chem. Rev."},{"issue":"5","key":"10.1016\/j.gene.2024.149160_b0065","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1038\/nrg3664","article-title":"Genomic insights into tuberculosis","volume":"15","author":"Galagan","year":"2014","journal-title":"Nat. Rev. Genet."},{"key":"10.1016\/j.gene.2024.149160_b0070","first-page":"176","article-title":"jefA (Rv2459), a drug efflux gene in Mycobacterium tuberculosis confers resistance to isoniazid & ethambutol","volume":"132","author":"Gupta","year":"2010","journal-title":"Indian J. Med. Res."},{"issue":"2","key":"10.1016\/j.gene.2024.149160_b0075","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1002\/1097-0134(20001101)41:2<224::AID-PROT70>3.0.CO;2-Z","article-title":"Rapid automatic detection and alignment of repeats in protein sequences","volume":"41","author":"Heger","year":"2000","journal-title":"Proteins Struct. Funct. Genet."},{"issue":"22","key":"10.1016\/j.gene.2024.149160_b0080","doi-asserted-by":"crossref","first-page":"10915","DOI":"10.1073\/pnas.89.22.10915","article-title":"Amino acid substitution matrices from protein blocks","volume":"89","author":"Henikoff","year":"1992","journal-title":"Proc. Natl. Acad. Sci."},{"issue":"6","key":"10.1016\/j.gene.2024.149160_b0085","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1038\/nprot.2015.053","article-title":"The Phyre2 web portal for protein modeling, prediction and analysis","volume":"10","author":"Kelley","year":"2015","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.gene.2024.149160_b0090","unstructured":"L., D. W. (2002). The PyMOL molecular graphics system. Http:\/\/Www.Pymol.Org\/. https:\/\/cir.nii.ac.jp\/crid\/1570572701247244160."},{"issue":"2","key":"10.1016\/j.gene.2024.149160_b0095","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1107\/S0021889892009944","article-title":"PROCHECK: a program to check the stereochemical quality of protein structures","volume":"26","author":"Laskowski","year":"1993","journal-title":"J. Appl. Cryst."},{"issue":"1","key":"10.1016\/j.gene.2024.149160_b0100","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.tim.2021.05.001","article-title":"Efflux pumps in Mycobacterium tuberculosis and their inhibition to tackle antimicrobial resistance","volume":"30","author":"Laws","year":"2022","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.gene.2024.149160_b0105","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkae268","article-title":"Interactive Tree of Life (iTOL) v6: recent updates to the phylogenetic tree display and annotation tool","author":"Letunic","year":"2024","journal-title":"Nucleic Acids Res."},{"issue":"6","key":"10.1016\/j.gene.2024.149160_b0110","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1007\/s00232-017-9982-x","article-title":"Mycobacterium tuberculosis major facilitator superfamily transporters","volume":"250","author":"Li","year":"2017","journal-title":"J. Membr. Biol."},{"issue":"7","key":"10.1016\/j.gene.2024.149160_b0115","doi-asserted-by":"crossref","first-page":"2415","DOI":"10.1128\/AAC.48.7.2415-2423.2004","article-title":"Efflux pump-mediated intrinsic drug resistance in Mycobacterium smegmatis","volume":"48","author":"Li","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"issue":"4","key":"10.1016\/j.gene.2024.149160_b0120","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.diagmicrobio.2016.05.010","article-title":"Molecular analysis of genetic mutations among cross-resistant second-line injectable drugs reveals a new resistant mutation in Mycobacterium tuberculosis","volume":"85","author":"Malinga","year":"2016","journal-title":"Diagn. Microbiol. Infect. Dis."},{"issue":"7","key":"10.1016\/j.gene.2024.149160_b0125","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/j.tim.2020.10.013","article-title":"Heterogeneous host\u2013pathogen encounters coordinate antibiotic resilience in mycobacterium tuberculosis","volume":"29","author":"Mishra","year":"2021","journal-title":"Trends Microbiol."},{"issue":"1","key":"10.1016\/j.gene.2024.149160_b0130","doi-asserted-by":"crossref","DOI":"10.1002\/0471250953.bi0207s21","article-title":"The InterPro database and tools for protein domain analysis","volume":"21","author":"Mulder","year":"2008","journal-title":"Current Protocols in Bioinformatics"},{"key":"10.1016\/j.gene.2024.149160_b0135","doi-asserted-by":"crossref","DOI":"10.3389\/fmolb.2021.723274","article-title":"Host - Bacterial pathogen communication: the wily role of the multidrug efflux pumps of the MFS family","volume":"8","author":"Pasqua","year":"2021","journal-title":"Front. Mol. Biosci."},{"issue":"9","key":"10.1016\/j.gene.2024.149160_b0140","doi-asserted-by":"crossref","first-page":"285","DOI":"10.3390\/microorganisms7090285","article-title":"The varied role of efflux pumps of the MFS family in the interplay of bacteria with animal and plant cells","volume":"7","author":"Pasqua","year":"2019","journal-title":"Microorganisms"},{"issue":"9","key":"10.1016\/j.gene.2024.149160_b0145","doi-asserted-by":"crossref","first-page":"3675","DOI":"10.1128\/AAC.00550-09","article-title":"Role of the Mycobacterium tuberculosis P55 efflux pump in intrinsic drug resistance, oxidative stress responses, and growth","volume":"53","author":"Ram\u00f3n-Garc\u00eda","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"issue":"4","key":"10.1016\/j.gene.2024.149160_b0150","doi-asserted-by":"crossref","first-page":"2074","DOI":"10.1128\/AAC.05946-11","article-title":"Functional and genetic characterization of the tap efflux pump in mycobacterium bovis BCG","volume":"56","author":"Ram\u00f3n-Garc\u00eda","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"issue":"4","key":"10.1016\/j.gene.2024.149160_b0155","doi-asserted-by":"crossref","DOI":"10.1093\/femsyr\/fow043","article-title":"MFS transporters of Candida species and their role in clinical drug resistance","volume":"16","author":"Redhu","year":"2016","journal-title":"FEMS Yeast Res."},{"issue":"1","key":"10.1016\/j.gene.2024.149160_b0160","doi-asserted-by":"crossref","DOI":"10.1093\/femsre\/fuab050","article-title":"Critical discussion on drug efflux in Mycobacterium tuberculosis","volume":"46","author":"Remm","year":"2022","journal-title":"FEMS Microbiol. Rev."},{"issue":"1","key":"10.1016\/j.gene.2024.149160_b0165","doi-asserted-by":"crossref","first-page":"6449","DOI":"10.1038\/s41467-023-42073-0","article-title":"Structural basis for triacylglyceride extraction from mycobacterial inner membrane by MFS transporter Rv1410","volume":"14","author":"Remm","year":"2023","journal-title":"Nat. Commun."},{"issue":"1","key":"10.1016\/j.gene.2024.149160_b0170","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1046\/j.1365-2958.2003.03425.x","article-title":"Genes required for mycobacterial growth defined by high density mutagenesis","volume":"48","author":"Sassetti","year":"2003","journal-title":"Mol. Microbiol."},{"issue":"8","key":"10.1016\/j.gene.2024.149160_b0175","doi-asserted-by":"crossref","first-page":"1694","DOI":"10.1093\/jac\/dkq186","article-title":"Piperine as an inhibitor of Rv1258c, a putative multidrug efflux pump of Mycobacterium tuberculosis","volume":"65","author":"Sharma","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"issue":"3","key":"10.1016\/j.gene.2024.149160_b0180","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1128\/AAC.45.3.800-804.2001","article-title":"Characterization of P55, a multidrug efflux pump in mycobacterium bovis and mycobacterium tuberculosis","volume":"45","author":"Silva","year":"2001","journal-title":"Antimicrob. Agents Chemother."},{"issue":"1","key":"10.1016\/j.gene.2024.149160_b0185","doi-asserted-by":"crossref","DOI":"10.1128\/jb.00337-22","article-title":"Mycobacterium smegmatis: the vanguard of mycobacterial research","volume":"205","author":"Sparks","year":"2023","journal-title":"J. Bacteriol."},{"issue":"7","key":"10.1016\/j.gene.2024.149160_b0190","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: molecular evolutionary genetics analysis version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"issue":"1","key":"10.1016\/j.gene.2024.149160_b0195","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1146\/annurev-pharmtox-010617-052438","article-title":"The role of efflux pumps in tuberculosis treatment and their promise as a target in drug development: unraveling the black box","volume":"58","author":"te Brake","year":"2018","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"issue":"4","key":"10.1016\/j.gene.2024.149160_b0200","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.tim.2010.12.008","article-title":"The rise and fall of the Mycobacterium tuberculosis genome","volume":"19","author":"Veyrier","year":"2011","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.gene.2024.149160_b0205","first-page":"1","article-title":"Characterization of a Mycobacterium avium subsp. avium operon associated with virulence and drug detoxification","volume":"2014","author":"Viale","year":"2014","journal-title":"Biomed Res. Int."},{"issue":"11","key":"10.1016\/j.gene.2024.149160_b0210","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1007\/s13205-018-1476-8","article-title":"Phylogenetic and conservation analyses of MFS transporters","volume":"8","author":"Vishwakarma","year":"2018","journal-title":"3 Biotech"},{"issue":"9","key":"10.1016\/j.gene.2024.149160_b0215","doi-asserted-by":"crossref","DOI":"10.1016\/j.bbamem.2020.183277","article-title":"Expansion of the Major Facilitator Superfamily (MFS) to include novel transporters as well as transmembrane-acting enzymes","volume":"1862","author":"Wang","year":"2020","journal-title":"Biochim. Biophys. Acta Biomembr."},{"issue":"3","key":"10.1016\/j.gene.2024.149160_b0220","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.tibs.2013.01.003","article-title":"Structural advances for the major facilitator superfamily (MFS) transporters","volume":"38","author":"Yan","year":"2013","journal-title":"Trends Biochem. Sci."},{"issue":"10","key":"10.1016\/j.gene.2024.149160_b0225","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1002\/pro.2759","article-title":"Energy coupling mechanisms of <scp>MFS<\/scp> transporters","volume":"24","author":"Zhang","year":"2015","journal-title":"Protein Sci."}],"container-title":["Gene"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378111924010412?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378111924010412?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,12,26]],"date-time":"2024-12-26T17:37:05Z","timestamp":1735234625000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378111924010412"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":45,"alternative-id":["S0378111924010412"],"URL":"https:\/\/doi.org\/10.1016\/j.gene.2024.149160","relation":{},"ISSN":["0378-1119"],"issn-type":[{"value":"0378-1119","type":"print"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"From ancestor to pathogen: Expansion and evolutionary adaptations of multidrug resistance causing MFS efflux pumps in mycobacteria","name":"articletitle","label":"Article Title"},{"value":"Gene","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.gene.2024.149160","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"149160"}}