{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T05:33:02Z","timestamp":1787981582793,"version":"build-2784847793"},"reference-count":27,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute\/National Institutes of Health","doi-asserted-by":"publisher","award":["R01 EY019875"],"award-info":[{"award-number":["R01 EY019875"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health & Medical Research Council of Australia","doi-asserted-by":"publisher","award":["APP1123684"],"award-info":[{"award-number":["APP1123684"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["FT130101648"],"award-info":[{"award-number":["FT130101648"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001108","name":"The Ophthalmic Research Institute of Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Gene Reports"],"published-print":{"date-parts":[[2018,3]]},"DOI":"10.1016\/j.genrep.2017.11.009","type":"journal-article","created":{"date-parts":[[2017,11,24]],"date-time":"2017-11-24T20:47:16Z","timestamp":1511556436000},"page":"123-134","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":11,"special_numbering":"C","title":["Selection of reference genes for studies of human retinal endothelial cell gene expression by reverse transcription-quantitative real-time polymerase chain reaction"],"prefix":"10.1016","volume":"10","author":[{"given":"Binoy","family":"Appukuttan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liam M.","family":"Ashander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuefang","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justine R.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.genrep.2017.11.009_bb0005","doi-asserted-by":"crossref","DOI":"10.1155\/2016\/7945848","article-title":"Targeting endothelial adhesion molecule transcription for treatment of inflammatory disease: a proof-of-concept study","volume":"2016","author":"Ashander","year":"2016","journal-title":"Mediat. Inflamm."},{"key":"10.1016\/j.genrep.2017.11.009_bb0010","doi-asserted-by":"crossref","first-page":"2159","DOI":"10.1534\/g3.114.013102","article-title":"Reference genes for expression studies in hypoxia and hyperglycemia models in human umbilical vein endothelial cells","volume":"4","author":"Bakhashab","year":"2014","journal-title":"G3."},{"key":"10.1016\/j.genrep.2017.11.009_bb0015","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0139101","article-title":"Inflammation modulates RLIP76\/RALBP1 electrophile-glutathione conjugate transporter and housekeeping genes in human blood-brain barrier endothelial cells","volume":"10","author":"Bennani-Baiti","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.genrep.2017.11.009_bb0020","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.preteyeres.2012.08.004","article-title":"Role of the retinal vascular endothelial cell in ocular disease","volume":"32","author":"Bharadwaj","year":"2013","journal-title":"Prog. Retin. Eye Res."},{"key":"10.1016\/j.genrep.2017.11.009_bb0025","doi-asserted-by":"crossref","first-page":"6917","DOI":"10.1167\/iovs.13-12058","article-title":"Intercellular adhesion molecule 1 mediates migration of Th1 and Th17 cells across human retinal vascular endothelium","volume":"54","author":"Bharadwaj","year":"2013","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.genrep.2017.11.009_bb0030","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1136\/bjophthalmol-2011-300572","article-title":"Comparative gene expression profiling of human umbilical vein endothelial cells and ocular vascular endothelial cells","volume":"96","author":"Browning","year":"2012","journal-title":"Br. J. Ophthalmol."},{"key":"10.1016\/j.genrep.2017.11.009_bb0035","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin. Chem."},{"key":"10.1016\/j.genrep.2017.11.009_bb0040","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1038\/nmeth.2697","article-title":"The need for transparency and good practices in the qPCR literature","volume":"10","author":"Bustin","year":"2013","journal-title":"Nat. Methods"},{"key":"10.1016\/j.genrep.2017.11.009_bb0045","article-title":"Validation of reliable reference genes for real-time PCR in human umbilical vein endothelial cells on substrates with different stiffness","volume":"8","author":"Chen","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.genrep.2017.11.009_bb0050","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1038\/icb.2012.21","article-title":"Toxoplasma gondii tachyzoites cross retinal endothelium assisted by intercellular adhesion molecule-1 in vitro","volume":"90","author":"Furtado","year":"2012","journal-title":"Immunol. Cell Biol."},{"key":"10.1016\/j.genrep.2017.11.009_bb0055","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.jim.2010.02.006","article-title":"Housekeeping genes; expression levels may change with density of cultured cells","volume":"355","author":"Greer","year":"2010","journal-title":"J. Immunol. Methods"},{"key":"10.1016\/j.genrep.2017.11.009_bb0060","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1128\/jvi.65.1.473-478.1991","article-title":"The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells","volume":"65","author":"Halbert","year":"1991","journal-title":"J. Virol."},{"key":"10.1016\/j.genrep.2017.11.009_bb0065","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1101\/gr.6.10.986","article-title":"Real time quantitative PCR","volume":"6","author":"Heid","year":"1996","journal-title":"Genome Res."},{"key":"10.1016\/j.genrep.2017.11.009_bb0070","doi-asserted-by":"crossref","first-page":"R19","DOI":"10.1186\/gb-2007-8-2-r19","article-title":"qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data","volume":"8","author":"Hellemans","year":"2007","journal-title":"Genome Biol."},{"key":"10.1016\/j.genrep.2017.11.009_bb0075","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0059180","article-title":"Careful selection of reference genes is required for reliable performance of RT-qPCR in human normal and cancer cell lines","volume":"8","author":"Jacob","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.genrep.2017.11.009_bb0080","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1128\/EC.00150-09","article-title":"Phenotypic and gene expression changes among clonal type I strains of Toxoplasma gondii","volume":"8","author":"Khan","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"10.1016\/j.genrep.2017.11.009_bb0085","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s12867-017-0086-z","article-title":"Identification of suitable reference genes for real-time quantitative PCR analysis of hydrogen peroxide-treated human umbilical vein endothelial cells","volume":"18","author":"Li","year":"2017","journal-title":"BMC Mol. Biol."},{"key":"10.1016\/j.genrep.2017.11.009_bb0090","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(\u2212Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.genrep.2017.11.009_bb0095","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1097\/APO.0000000000000109","article-title":"Ubiquitin carboxyl-terminal esterase L1 promotes proliferation of human choroidal and retinal endothelial cells","volume":"4","author":"Pan","year":"2015","journal-title":"Asia Pac J. Ophthalmol."},{"key":"10.1016\/j.genrep.2017.11.009_bb0100","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0165-022X(00)00129-9","article-title":"Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR","volume":"46","author":"Schmittgen","year":"2000","journal-title":"J. Biochem. Biophys. Methods"},{"key":"10.1016\/j.genrep.2017.11.009_bb0105","doi-asserted-by":"crossref","first-page":"2676","DOI":"10.1167\/iovs.06-0598","article-title":"Unique gene expression profiles of donor-matched human retinal and choroidal vascular endothelial cells","volume":"48","author":"Smith","year":"2007","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.genrep.2017.11.009_bb0110","doi-asserted-by":"crossref","first-page":"8232","DOI":"10.1167\/iovs.14-14217","article-title":"Modulation of VEGF-induced retinal vascular permeability by peroxisome proliferator-activated receptor-beta\/delta","volume":"55","author":"Suarez","year":"2014","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"10.1016\/j.genrep.2017.11.009_bb0115","doi-asserted-by":"crossref","DOI":"10.1186\/gb-2002-3-7-research0034","article-title":"Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes","volume":"3","author":"Vandesompele","year":"2002","journal-title":"Genome biology"},{"key":"10.1016\/j.genrep.2017.11.009_bb0120","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/1756-0500-6-46","article-title":"Suitability of endogenous reference genes for gene expression studies with human intraocular endothelial cells","volume":"6","author":"Wei","year":"2013","journal-title":"BMC Res Notes."},{"issue":"1","key":"10.1016\/j.genrep.2017.11.009_bb0125","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.gene.2016.03.047","article-title":"Validation of RT-qPCR reference genes and determination of Robo4 expression levels in human retinal endothelial cells under hypoxia and\/or hyperglycemia","volume":"585","author":"Xie","year":"2016","journal-title":"Gene"},{"key":"10.1016\/j.genrep.2017.11.009_bb0130","doi-asserted-by":"crossref","DOI":"10.1155\/2016\/3958453","article-title":"miR-146a attenuates inflammatory pathways mediated by TLR4\/NF-kappaB and TNFalpha to protect primary human retinal microvascular endothelial cells grown in high glucose","volume":"2016","author":"Ye","year":"2016","journal-title":"Mediat. Inflamm."},{"key":"10.1016\/j.genrep.2017.11.009_bb0135","first-page":"2058","article-title":"Proteomic profiling of human retinal and choroidal endothelial cells reveals molecular heterogeneity related to tissue of origin","volume":"13","author":"Zamora","year":"2007","journal-title":"Mol. Vis."}],"container-title":["Gene Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2452014417300924?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2452014417300924?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T15:07:41Z","timestamp":1762528061000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2452014417300924"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3]]},"references-count":27,"alternative-id":["S2452014417300924"],"URL":"https:\/\/doi.org\/10.1016\/j.genrep.2017.11.009","relation":{},"ISSN":["2452-0144"],"issn-type":[{"value":"2452-0144","type":"print"}],"subject":[],"published":{"date-parts":[[2018,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Selection of reference genes for studies of human retinal endothelial cell gene expression by reverse transcription-quantitative real-time polymerase chain reaction","name":"articletitle","label":"Article Title"},{"value":"Gene Reports","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.genrep.2017.11.009","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Inc. All rights reserved.","name":"copyright","label":"Copyright"}]}}