{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T04:34:19Z","timestamp":1791606859905,"version":"4.3.4"},"reference-count":89,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Harmful Algae"],"published-print":{"date-parts":[[2023,3]]},"DOI":"10.1016\/j.hal.2023.102391","type":"journal-article","created":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T13:08:21Z","timestamp":1675688901000},"page":"102391","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":13,"special_numbering":"C","title":["Transcriptomic analysis of polyketide synthesis in dinoflagellate, Prorocentrum lima"],"prefix":"10.1016","volume":"123","author":[{"given":"Xiukun","family":"Wan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoheng","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penggang","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuli","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyu","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.hal.2023.102391_bib0001","doi-asserted-by":"crossref","first-page":"97","DOI":"10.3390\/toxins10030097","article-title":"Prorocentrolide-a from cultured Prorocentrum lima dinoflagellates collected in Japan blocks sub-types of nicotinic acetylcholine receptors","volume":"10","author":"Amar","year":"2018","journal-title":"Toxins"},{"issue":"1","key":"10.1016\/j.hal.2023.102391_bib0002","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s10750-018-3512-4","article-title":"Effect of increasing sea water temperature on the growth and toxin production of the benthic dinoflagellate Prorocentrum lima","volume":"813","author":"Aquino-Cruz","year":"2018","journal-title":"Hydrobiologia"},{"issue":"4","key":"10.1016\/j.hal.2023.102391_bib0003","doi-asserted-by":"crossref","first-page":"e35269","DOI":"10.1371\/journal.pone.0035269","article-title":"Symbiodinium transcriptomes: genome insights into the dinoflagellate symbionts of reef-building corals","volume":"7","author":"Bayer","year":"2012","journal-title":"PLoS ONE"},{"issue":"1","key":"10.1016\/j.hal.2023.102391_bib0004","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12864-015-2195-8","article-title":"Multifunctional polyketide synthase genes identified by genomic survey of the symbiotic dinoflagellate, Symbiodinium minutum","volume":"16","author":"Beedessee","year":"2015","journal-title":"BMC Genomics"},{"issue":"1","key":"10.1016\/j.hal.2023.102391_bib0005","first-page":"1","article-title":"Diversified secondary metabolite biosynthesis gene repertoire revealed in symbiotic dinoflagellates","volume":"9","author":"Beedessee","year":"2019","journal-title":"Sci. Rep-UK."},{"key":"10.1016\/j.hal.2023.102391_bib0006","doi-asserted-by":"crossref","first-page":"2307","DOI":"10.1007\/s00018-008-8019-0","article-title":"The leucine-rich repeat structure","volume":"65","author":"Bella","year":"2008","journal-title":"Cell. Mol. Life Sci."},{"issue":"10","key":"10.1016\/j.hal.2023.102391_bib0007","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1016\/S0041-0101(01)00126-X","article-title":"Toxin composition of the toxic dinoflagellate Prorocentrum lima isolated from different locations along the Galician coast (NW Spain)","volume":"39","author":"Bravo","year":"2001","journal-title":"Toxicon"},{"issue":"6409","key":"10.1016\/j.hal.2023.102391_bib0008","doi-asserted-by":"crossref","first-page":"1356","DOI":"10.1126\/science.aau0382","article-title":"Biosynthesis of the neurotoxin domoic acid in a bloom-forming diatom","volume":"361","author":"Brunson","year":"2018","journal-title":"Science"},{"issue":"1","key":"10.1016\/j.hal.2023.102391_bib0009","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.mycres.2008.08.007","article-title":"A transcribed polyketide synthase gene from Xanthoria elegans","volume":"113","author":"Brunauer","year":"2009","journal-title":"Mycol. Res."},{"key":"10.1016\/j.hal.2023.102391_bib0010","doi-asserted-by":"crossref","DOI":"10.3389\/fmars.2021.716669","article-title":"Phylogeography and diversity among populations of the toxigenic benthic dinoflagellate Prorocentrum from coastal reef systems in Mexico","volume":"8","author":"Cembella","year":"2021","journal-title":"Front. Mar. Sci."},{"issue":"3","key":"10.1016\/j.hal.2023.102391_bib0011","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0041-0101(95)00141-7","article-title":"Dinophysistoxin-2: the predominant diarrhoetic shellfish toxin in Ireland","volume":"34","author":"Carmody","year":"1996","journal-title":"Toxicon"},{"issue":"17","key":"10.1016\/j.hal.2023.102391_bib0012","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: an ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"issue":"7","key":"10.1016\/j.hal.2023.102391_bib0013","first-page":"1","article-title":"Corset: enabling differential gene expression analysis for de novoassembled transcriptomes","volume":"15","author":"Davidson","year":"2014","journal-title":"Genome Biol."},{"issue":"11","key":"10.1016\/j.hal.2023.102391_bib0014","doi-asserted-by":"crossref","first-page":"e48624","DOI":"10.1371\/journal.pone.0048624","article-title":"Putative monofunctional type I polyketide synthase units: a dinoflagellate-specific feature?","volume":"7","author":"Eichholz","year":"2012","journal-title":"PLoS ONE"},{"issue":"46","key":"10.1016\/j.hal.2023.102391_bib0015","doi-asserted-by":"crossref","first-page":"11779","DOI":"10.1002\/ange.201205566","article-title":"Genomics\u2013driven discovery of burkholderic acid, a noncanonical, cryptic polyketide from human pathogenic Burkholderia species","volume":"124","author":"Franke","year":"2012","journal-title":"Angew. Chem. Int. Ed."},{"issue":"3","key":"10.1016\/j.hal.2023.102391_bib0016","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1007\/s004320050257","article-title":"Unique features of the okadaic acid activity class of tumor promoters","volume":"125","author":"Fujiki","year":"1999","journal-title":"J. Cancer. Res. Clin."},{"key":"10.1016\/j.hal.2023.102391_bib0017","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.crbiot.2021.01.001","article-title":"Prediction of GPI-anchored proteins with pointer neural networks","volume":"3","author":"G\u00edslason","year":"2021","journal-title":"Curr. Res. Biotechnol."},{"issue":"7","key":"10.1016\/j.hal.2023.102391_bib0018","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length transcriptome assembly from RNA-Seq data without a reference genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.hal.2023.102391_bib0019","doi-asserted-by":"crossref","DOI":"10.1016\/j.hal.2021.102025","article-title":"Determination of optimal culture conditions for toxin production by a Prorocentrum lima complex strain with high diarrhetic shellfish toxins yield","volume":"103","author":"Hashimoto","year":"2021","journal-title":"Harmful Algae"},{"issue":"2","key":"10.1016\/j.hal.2023.102391_bib0020","first-page":"151","article-title":"A high level of thymine replacement by 5-hydroxymethyluracil in nuclear DNA of the primitive dinoflagellate Prorocentrum micans E","volume":"27","author":"Herzog","year":"1982","journal-title":"Eur. J. Cell. Biol."},{"key":"10.1016\/j.hal.2023.102391_bib0021","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.hal.2016.04.005","article-title":"Physiological and transcriptional responses to inorganic nutrition in a tropical Pacific strain of Alexandrium minutum: implications for the saxitoxin genes and toxin production","volume":"56","author":"Hii","year":"2016","journal-title":"Harmful Algae"},{"issue":"9","key":"10.1016\/j.hal.2023.102391_bib0022","doi-asserted-by":"crossref","first-page":"e6978","DOI":"10.1371\/journal.pone.0006978","article-title":"Distinct gene number-genome size relationships for eukaryotes and non-eukaryotes: gene content estimation for dinoflagellate genomes","volume":"4","author":"Hou","year":"2009","journal-title":"PLoS ONE"},{"key":"10.1016\/j.hal.2023.102391_bib0023","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.algal.2018.06.004","article-title":"Systems-level analysis of metabolic mechanism following nitrogen limitation in benthic dinoflagellate Prorocentrum lima","volume":"33","author":"Hou","year":"2018","journal-title":"Algal. Res."},{"issue":"3","key":"10.1016\/j.hal.2023.102391_bib0024","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1007\/s10811-015-0679-1","article-title":"MRP functional activity and character in the dinoflagellate Prorocentrum lima","volume":"28","author":"Hou","year":"2016","journal-title":"J. Appl. Phycol."},{"key":"10.1016\/j.hal.2023.102391_bib0025","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.hal.2017.01.012","article-title":"Sulfated diesters of okadaic acid and DTX-1: self-protective precursors of diarrhetic shellfish poisoning (DSP) toxins","volume":"63","author":"Hu","year":"2017","journal-title":"Harmful Algae"},{"issue":"1","key":"10.1016\/j.hal.2023.102391_bib0026","doi-asserted-by":"crossref","first-page":"51","DOI":"10.2174\/138955710791112541","article-title":"Polyketides from marine dinoflagellates of the genus Prorocentrum, biosynthetic origin and bioactivity of their okadaic acid analogues","volume":"10","author":"Hu","year":"2010","journal-title":"Mini-Rev. Med. Chem."},{"issue":"10","key":"10.1016\/j.hal.2023.102391_bib0027","doi-asserted-by":"crossref","first-page":"2027","DOI":"10.1093\/molbev\/msi193","article-title":"Evolutionary implications of bacterial polyketide synthases","volume":"22","author":"Jenke-Kodama","year":"2005","journal-title":"Mol. Biol. Evol."},{"issue":"1","key":"10.1016\/j.hal.2023.102391_bib0028","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.protis.2007.08.001","article-title":"Novel insights into evolution of protistan polyketide synthases through phylogenomic analysis","volume":"159","author":"John","year":"2008","journal-title":"Protist"},{"issue":"11","key":"10.1016\/j.hal.2023.102391_bib0029","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0142731","article-title":"RNA sequencing revealed numerous polyketide synthase genes in the harmful dinoflagellate Karenia mikimotoi","volume":"10","author":"Kimura","year":"2015","journal-title":"PLoS ONE"},{"issue":"6","key":"10.1016\/j.hal.2023.102391_bib0030","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1016\/S0959-440X(01)00266-4","article-title":"The leucine-rich repeat as a protein recognition motif","volume":"11","author":"Kobe","year":"2001","journal-title":"Curr. Opin. Struc. Biol."},{"issue":"1","key":"10.1016\/j.hal.2023.102391_bib0031","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12864-015-1625-y","article-title":"Polyketide synthesis genes associated with toxin production in two species of Gambierdiscus (Dinophyceae)","volume":"16","author":"Kohli","year":"2015","journal-title":"BMC Genomics"},{"issue":"8","key":"10.1016\/j.hal.2023.102391_bib0032","doi-asserted-by":"crossref","first-page":"1877","DOI":"10.1038\/ismej.2015.263","article-title":"Evolutionary distinctiveness of fatty acid and polyketide synthesis in eukaryotes","volume":"10","author":"Kohli","year":"2016","journal-title":"ISME. J."},{"issue":"5","key":"10.1016\/j.hal.2023.102391_bib0033","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1111\/jeu.12405","article-title":"Role of modular polyketide synthases in the production of polyether ladder compounds in Ciguatoxin-producing Gambierdiscus polynesiensis and G. excentricus (Dinophyceae)","volume":"64","author":"Kohli","year":"2017","journal-title":"J. Eukaryot. Microbiol."},{"issue":"1","key":"10.1016\/j.hal.2023.102391_bib0034","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1002\/tox.20154","article-title":"Genotoxicity of the marine toxin okadaic acid, in human Caco-2 cells and in mice gut cells","volume":"21","author":"Le H\u00e9garat","year":"2006","journal-title":"Environ. Toxicol."},{"issue":"10","key":"10.1016\/j.hal.2023.102391_bib0035","doi-asserted-by":"crossref","first-page":"272","DOI":"10.3390\/toxins8100272","article-title":"The mechanism of diarrhetic shellfish poisoning toxin production in Prorocentrum spp.: physiological and molecular perspectives","volume":"8","author":"Lee","year":"2016","journal-title":"Toxins"},{"issue":"6261","key":"10.1016\/j.hal.2023.102391_bib0036","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1126\/science.aad0408","article-title":"The Symbiodinium kawagutii genome illuminates dinoflagellate gene expression and coral symbiosis","volume":"350","author":"Lin","year":"2015","journal-title":"Science"},{"issue":"12","key":"10.1016\/j.hal.2023.102391_bib0037","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"issue":"9","key":"10.1016\/j.hal.2023.102391_bib0038","doi-asserted-by":"crossref","first-page":"1713","DOI":"10.1016\/S0040-4039(00)02331-5","article-title":"Spiro-prorocentrimine, a novel macrocyclic lactone from a benthic Prorocentrum sp. of Taiwan","volume":"42","author":"Lu","year":"2001","journal-title":"Tetrahedron. Lett."},{"issue":"18","key":"10.1016\/j.hal.2023.102391_bib0039","doi-asserted-by":"crossref","first-page":"3893","DOI":"10.1021\/ol051300u","article-title":"Prorocentin, a new polyketide from the marine dinoflagellate prorocentrum lima","volume":"7","author":"Lu","year":"2005","journal-title":"Org. let."},{"issue":"16","key":"10.1016\/j.hal.2023.102391_bib0040","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.1016\/j.tetlet.2009.02.013","article-title":"Formosalides A and B, cytotoxic 17-membered ring macrolides from a marine dinoflagellate Prorocentrum sp","volume":"50","author":"Lu","year":"2009","journal-title":"Tetrahedron. Lett."},{"key":"10.1016\/j.hal.2023.102391_bib0041","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.2331\/fishsci.68.sup2_1593","article-title":"Research of marine dinoflagellate bioactive compounds in Taiwan","volume":"68","author":"Lu","year":"2002","journal-title":"Fish. Sci."},{"issue":"5","key":"10.1016\/j.hal.2023.102391_bib0042","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1002\/nt.2620020504","article-title":"Effects of nitrogen concentration and cold temperature on DSP toxin concentrations in the dinoflagellate Prorocentrum lima (Prorocentrales, Dinophyceae)","volume":"2","author":"McLachlan","year":"1994","journal-title":"Nat. Toxins"},{"issue":"1","key":"10.1016\/j.hal.2023.102391_bib0043","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12864-014-1205-6","article-title":"Transcriptomic characterisation and genomic glimps into the toxigenic dinoflagellate Azadinium spinosum, with emphasis on polykeitde synthase genes","volume":"16","author":"Meyer","year":"2015","journal-title":"BMC Genomics"},{"issue":"3","key":"10.1016\/j.hal.2023.102391_bib0044","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.protis.2008.02.004","article-title":"The toxic dinoflagellate Karenia brevis encodes novel type I-like polyketide synthases containing discrete catalytic domains","volume":"159","author":"Monroe","year":"2008","journal-title":"Protist"},{"key":"10.1016\/j.hal.2023.102391_bib0045","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1111\/j.1529-8817.2010.00837.x","article-title":"Characterization and expression of nuclear-encoded polyketide synthases in the brevetoxin-producing dinoflagellate Karenia brevis","volume":"46","author":"Monroe","year":"2010","journal-title":"J. Phycol."},{"issue":"1","key":"10.1016\/j.hal.2023.102391_bib0046","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1021\/ja971547p","article-title":"Labeling pattern of okadaic acid from 18O2 and [18O2] acetate elucidated by collision-induced dissociation tandem mass spectrometry","volume":"120","author":"Murata","year":"1998","journal-title":"J. Am. Chem. Soc."},{"issue":"1","key":"10.1016\/j.hal.2023.102391_bib0047","doi-asserted-by":"crossref","first-page":"69","DOI":"10.2331\/suisan.48.69","article-title":"Identification of Okadaic Acid as a toxic component of a marine dinoflagellate Prorocentrum lima","volume":"48","author":"Murakami","year":"1982","journal-title":"Bull. Japanese Soc. Sci. Fish."},{"issue":"5","key":"10.1016\/j.hal.2023.102391_bib0048","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.toxicon.2004.12.023","article-title":"Morphology, toxin composition and pigment content of Prorocentrum lima strains isolated from a coastal lagoon in southern UK","volume":"45","author":"Nascimento","year":"2005","journal-title":"Toxicon"},{"issue":"22","key":"10.1016\/j.hal.2023.102391_bib0049","doi-asserted-by":"crossref","first-page":"2599","DOI":"10.1039\/c39940002599","article-title":"Biosynthetic origin of C-37 and C-38 in the polyether toxins okadaic acid and dinophysistoxin-1","author":"Needham","year":"1994","journal-title":"J. Chem. Soc., Chem. Commun."},{"issue":"16","key":"10.1016\/j.hal.2023.102391_bib0050","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1039\/c39950001623","article-title":"Biosynthetic studies of the DSP toxin DTX-4 and an okadaic acid diol ester","author":"Needham","year":"1995","journal-title":"J. Chem. Soc., Chem. Commun."},{"issue":"9","key":"10.1016\/j.hal.2023.102391_bib0051","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1016\/S0040-4039(00)76241-1","article-title":"Studies on the biosynthesis of the polyether marine toxin dinophysistoxin-1 (DTX-1)","volume":"35","author":"Norte","year":"1994","journal-title":"Tetrahedron. Lett."},{"issue":"30","key":"10.1016\/j.hal.2023.102391_bib0052","doi-asserted-by":"crossref","first-page":"9175","DOI":"10.1016\/S0040-4020(01)85383-7","article-title":"Structural determination and biosynthetic origin of two ester derivatives of okadaic acid isolated from Prorocentrum lima","volume":"50","author":"Norte","year":"1994","journal-title":"Tetrahedron"},{"key":"10.1016\/j.hal.2023.102391_bib0053","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.toxicon.2021.03.017","article-title":"Taxonomy and okadaic acid production of a strain of Prorocentrum lima (Dinophyceae) isolated from the Bay of Bengal, North Indian Ocean","volume":"196","author":"Oyeku","year":"2021","journal-title":"Toxicon"},{"issue":"10","key":"10.1016\/j.hal.2023.102391_bib0054","doi-asserted-by":"crossref","first-page":"308","DOI":"10.3390\/toxins9100308","article-title":"Profiling of extracellular toxins associated with diarrhetic shellfish poison in Prorocentrum lima culture medium by high-performance liquid chromatography coupled with mass spectrometry","volume":"9","author":"Pan","year":"2017","journal-title":"Toxins"},{"key":"10.1016\/j.hal.2023.102391_bib0055","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.hal.2014.04.013","article-title":"Transcriptome sequencing reveals single domain Type I-like polyketide synthases in the toxic dinoflagellate Gambierdiscus polynesiensis","volume":"36","author":"Pawlowiez","year":"2014","journal-title":"Harmful Algae"},{"key":"10.1016\/j.hal.2023.102391_bib0056","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1039\/b816430b","article-title":"Biosynthesis of polyketides by trans-AT polyketide synthases","volume":"27","author":"Piel","year":"2010","journal-title":"Nat. Prod. Rep."},{"issue":"W1","key":"10.1016\/j.hal.2023.102391_bib0057","doi-asserted-by":"crossref","first-page":"W200","DOI":"10.1093\/nar\/gky448","article-title":"HMMER web server: 2018 update","volume":"46","author":"Potter","year":"2018","journal-title":"Nucleic Acids Res."},{"issue":"3","key":"10.1016\/j.hal.2023.102391_bib0058","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1021\/acs.jnatprod.5b01066","article-title":"Chemical diversity, origin, and analysis of phycotoxins","volume":"79","author":"Rasmussen","year":"2016","journal-title":"J. Nat. Prod."},{"issue":"2","key":"10.1016\/j.hal.2023.102391_bib0060","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S1367-5931(03)00020-6","article-title":"Polyketide biosynthesis beyond the type I, II and III polyketide synthase paradigms","volume":"7","author":"Shen","year":"2003","journal-title":"Curr. Opin. Chem. Boil."},{"key":"10.1016\/j.hal.2023.102391_bib0061","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1186\/s12864-015-2222-9","article-title":"Multimodular type I polyketide synthases in algae evolve by module duplications and displacement of AT domains in trans","volume":"16","author":"Shelest","year":"2015","journal-title":"BMC Genomics"},{"issue":"1","key":"10.1016\/j.hal.2023.102391_bib0062","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1002\/cbic.201600522","article-title":"Type III polyketide synthases: functional classification and phylogenomics","volume":"18","author":"Shimizu","year":"2017","journal-title":"ChemBioChem"},{"key":"10.1016\/j.hal.2023.102391_bib0064","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.hal.2012.06.008","article-title":"Toxin production, retention, and extracellular release by Dinophysis acuminata during extended stationary phase and culture decline","volume":"19","author":"Smith","year":"2012","journal-title":"Harmful Algae"},{"issue":"1","key":"10.1016\/j.hal.2023.102391_bib0065","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10126-002-0077-y","article-title":"Polyketide synthase genes from marine dinoflagellates","volume":"5","author":"Snyder","year":"2003","journal-title":"Mar. Biotechnol."},{"issue":"15","key":"10.1016\/j.hal.2023.102391_bib0067","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1016\/j.phytochem.2005.06.010","article-title":"Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis","volume":"66","author":"Snyder","year":"2005","journal-title":"Phytochemistry"},{"issue":"7","key":"10.1016\/j.hal.2023.102391_bib0068","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: molecular evolutionary genetics analysis version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.hal.2023.102391_bib0066","first-page":"631","article-title":"Changes in gene expression of Prymnesium parvum induced by nitrogen and phosphorus limitation","volume":"6","author":"Terrado","year":"2015","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.hal.2023.102391_bib0069","first-page":"1","article-title":"SignalP 6.0 predicts all five types of signal peptides using protein language models","author":"Teufel","year":"2022","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.hal.2023.102391_bib0070","doi-asserted-by":"crossref","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","article-title":"CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice","volume":"22","author":"Thompson","year":"1994","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.hal.2023.102391_bib0071","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gkac278","article-title":"DeepLoc 2.0: multi-label subcellular localization prediction using protein language models","author":"Thumuluri","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.hal.2023.102391_bib0072","doi-asserted-by":"crossref","first-page":"7876","DOI":"10.1021\/ja00231a048","article-title":"Prorocentrolide, a toxic nitrogenous macrocycle from a marine dinoflagellate, Prorocentrum lima","volume":"110","author":"Torigoe","year":"1988","journal-title":"J. Am. Chem. Soc."},{"issue":"2","key":"10.1016\/j.hal.2023.102391_bib0073","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1007\/s002040000188","article-title":"DNA breaks and cell cycle arrest induced by okadaic acid in Caco-2 cells, a human colonic epithelial cell line","volume":"75","author":"Traor\u00e9","year":"2001","journal-title":"Arch. Toxicol."},{"issue":"11","key":"10.1016\/j.hal.2023.102391_bib0074","doi-asserted-by":"crossref","first-page":"4328","DOI":"10.3390\/md11114328","article-title":"Okadaic acid: more than a diarrheic toxin","volume":"11","author":"Valdiglesias","year":"2013","journal-title":"Mar Drugs"},{"key":"10.1016\/j.hal.2023.102391_bib0075","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.toxicon.2009.03.026","article-title":"Toxin composition of a Prorocentrum lima strain isolated from the Portuguese coast","volume":"54","author":"Vale","year":"2009","journal-title":"Toxicon"},{"issue":"4","key":"10.1016\/j.hal.2023.102391_bib0076","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0231400","article-title":"Transcriptomic analysis of polyketide synthases in a highly ciguatoxic dinoflagellate, Gambierdiscus polynesiensis and low toxicity Gambierdiscus pacificus, from French Polynesia","volume":"15","author":"Van Dolah","year":"2020","journal-title":"PLoS ONE"},{"issue":"6","key":"10.1016\/j.hal.2023.102391_bib0077","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1111\/jpy.12586","article-title":"Both modular and single-domain Type I polyketide synthases are expressed in the brevetoxin-producing dinoflagellate, Karenia brevis (Dinophyceae)","volume":"53","author":"Van Dolah","year":"2017","journal-title":"J. Phycol."},{"issue":"6","key":"10.1016\/j.hal.2023.102391_bib0078","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1016\/j.hal.2010.04.009","article-title":"Effects of different levels of N-and P-deficiency on cell yield, okadaic acid, DTX-1, protein and carbohydrate dynamics in the benthic dinoflagellate Prorocentrum lima","volume":"9","author":"Vanucci","year":"2010","journal-title":"Harmful Algae"},{"issue":"3","key":"10.1016\/j.hal.2023.102391_bib0079","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.hal.2009.12.001","article-title":"Unbalanced N: p ratios and nutrient stress controlling growth and toxin production of the harmful dinoflagellate Prorocentrum lima (Ehrenberg) Dodge","volume":"9","author":"Varkitzi","year":"2010","journal-title":"Harmful Algae"},{"issue":"6","key":"10.1016\/j.hal.2023.102391_bib0080","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0157022","article-title":"SARTools: a DESeq2-and EdgeR-based R pipeline for comprehensive differential analysis of RNA-Seq data","volume":"11","author":"Varet","year":"2016","journal-title":"PLoS ONE"},{"issue":"11","key":"10.1016\/j.hal.2023.102391_bib0081","doi-asserted-by":"crossref","first-page":"4196","DOI":"10.1111\/1462-2920.14780","article-title":"Transcriptomic investigation into polyketide toxin synthesis in Ostreopsis (Dinophyceae) species","volume":"21","author":"Verma","year":"2019","journal-title":"Environ. Microbiol."},{"issue":"10","key":"10.1016\/j.hal.2023.102391_bib0082","doi-asserted-by":"crossref","first-page":"2234","DOI":"10.3390\/md10102234","article-title":"Biosynthetic studies on water-soluble derivative 5c (DTX5c)","volume":"10","author":"Vilches","year":"2012","journal-title":"Mar. Drugs"},{"issue":"8","key":"10.1016\/j.hal.2023.102391_bib0083","doi-asserted-by":"crossref","first-page":"386","DOI":"10.3390\/md18080386","article-title":"De novo transcriptome of the non-saxitoxin producing Alexandrium tamutum reveals new insights on harmful dinoflagellates","volume":"18","author":"Vingiani","year":"2020","journal-title":"Mar. Drugs"},{"issue":"10","key":"10.1016\/j.hal.2023.102391_bib0084","doi-asserted-by":"crossref","first-page":"594","DOI":"10.3390\/md17100594","article-title":"Research progress in the biosynthetic mechanisms of marine polyether toxins","volume":"17","author":"Wan","year":"2019","journal-title":"Mar. Drugs"},{"key":"10.1016\/j.hal.2023.102391_bib0085","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.biortech.2014.12.019","article-title":"Cultivation of the benthic microalga Prorocentrum lima for the production of diarrhetic shellfish poisoning toxins in a vertical flat photobioreactor","volume":"179","author":"Wang","year":"2015","journal-title":"Bioresource Technol."},{"key":"10.1016\/j.hal.2023.102391_bib0086","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0076-6879(09)04601-1","article-title":"Introduction to polyketide biosynthesis","volume":"459","author":"Weissman","year":"2009","journal-title":"Method. Enzymol."},{"issue":"Pt 1","key":"10.1016\/j.hal.2023.102391_bib0087","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1042\/bj3110017","article-title":"Serine\/threonine protein phosphatases","volume":"311","author":"Wera","year":"1995","journal-title":"Biochem. J."},{"key":"10.1016\/j.hal.2023.102391_bib0088","doi-asserted-by":"crossref","DOI":"10.1016\/j.scitotenv.2020.137184","article-title":"Characterizing ciguatoxin (CTX)-and Non-CTX-producing strains of Gambierdiscus balechii using comparative transcriptomics","volume":"717","author":"Wu","year":"2020","journal-title":"Sci. Total. Environ."},{"issue":"5","key":"10.1016\/j.hal.2023.102391_bib0089","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1021\/acs.jnatprod.7b00127","article-title":"Limaol: a polyketide from the benthic marine dinoflagellate Prorocentrum lima","volume":"80","author":"Yang","year":"2017","journal-title":"J. Nat. Prod."},{"issue":"4","key":"10.1016\/j.hal.2023.102391_bib0090","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1002\/iub.1005","article-title":"Type III polyketide synthases in natural product biosynthesis","volume":"64","author":"Yu","year":"2012","journal-title":"IUBMB Life"},{"key":"10.1016\/j.hal.2023.102391_bib0091","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1016\/j.scitotenv.2019.07.291","article-title":"Transcriptomic response to changing ambient phosphorus in the marine dinoflagellate Prorocentrum donghaiense","volume":"692","author":"Zhang","year":"2019","journal-title":"Sci. Total. Environ."}],"container-title":["Harmful Algae"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1568988323000185?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1568988323000185?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T21:09:45Z","timestamp":1761599385000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1568988323000185"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3]]},"references-count":89,"alternative-id":["S1568988323000185"],"URL":"https:\/\/doi.org\/10.1016\/j.hal.2023.102391","relation":{},"ISSN":["1568-9883"],"issn-type":[{"value":"1568-9883","type":"print"}],"subject":[],"published":{"date-parts":[[2023,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Transcriptomic analysis of polyketide synthesis in dinoflagellate, Prorocentrum lima","name":"articletitle","label":"Article Title"},{"value":"Harmful Algae","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.hal.2023.102391","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"102391"}}