{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:53:27Z","timestamp":1792788807975,"version":"4.5.0"},"reference-count":50,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"NIH","award":["R01-DC010146"],"award-info":[{"award-number":["R01-DC010146"]}]},{"name":"NIH","award":["R01-DC010397"],"award-info":[{"award-number":["R01-DC010397"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Hearing Research"],"published-print":{"date-parts":[[2016,5]]},"DOI":"10.1016\/j.heares.2016.02.019","type":"journal-article","created":{"date-parts":[[2016,2,28]],"date-time":"2016-02-28T12:18:28Z","timestamp":1456661908000},"page":"105-117","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":130,"special_numbering":"C","title":["HEI-OC1 cells as a model for investigating drug cytotoxicity"],"prefix":"10.1016","volume":"335","author":[{"given":"Gilda","family":"Kalinec","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pru","family":"Thein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Channy","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Kalinec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.heares.2016.02.019_bib1","series-title":"Empirical Model Building and Response Surfaces","author":"Box","year":"1987"},{"key":"10.1016\/j.heares.2016.02.019_bib2","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1146\/annurev.pharmtox.43.100901.140223","article-title":"Hormesis: the dose-response revolution","volume":"43","author":"Calabrese","year":"2003","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"10.1016\/j.heares.2016.02.019_bib3","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1146\/annurev-physiol-030212-183653","article-title":"Aging, cellular senescence, and cancer","volume":"75","author":"Campisi","year":"2013","journal-title":"Annu. Rev. Physiol."},{"key":"10.1016\/j.heares.2016.02.019_bib4","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1038\/nrm2233","article-title":"Cellular senescence: when bad things happen to good cells","volume":"8","author":"Campisi","year":"2007","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.heares.2016.02.019_bib5","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.heares.2012.08.011","article-title":"Loss of aminoglycoside sensitivity in HEI-OC1 cells?","volume":"292","author":"Cederroth","year":"2012","journal-title":"Hear. Res."},{"key":"10.1016\/j.heares.2016.02.019_bib6","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1146\/annurev-immunol-032414-112248","article-title":"Programmed necrosis in the cross talk of cell death and inflammation","volume":"33","author":"Chan","year":"2015","journal-title":"Annu. Rev. Immunol."},{"key":"10.1016\/j.heares.2016.02.019_bib7","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.heares.2011.12.007","article-title":"Activation of apoptotic pathways in the absence of cell death in an inner-ear immortomouse cell line","volume":"284","author":"Chen","year":"2012","journal-title":"Hear. Res."},{"key":"10.1016\/j.heares.2016.02.019_bib8","doi-asserted-by":"crossref","first-page":"1798","DOI":"10.1038\/nprot.2009.191","article-title":"Protocols to detect senescence-associated beta-galactosidase (SA-betagal) activity, a biomarker of senescent cells in culture and in\u00a0vivo","volume":"4","author":"Debacq-Chainiaux","year":"2009","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.heares.2016.02.019_bib9","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1038\/nrm2393","article-title":"Expansion and evolution of cell death programmes","volume":"9","author":"Degterev","year":"2008","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.heares.2016.02.019_bib10","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0378-5955(02)00634-2","article-title":"Cisplatin-induced apoptosis in auditory cells: role of death receptor and mitochondrial pathways","volume":"174","author":"Devarajan","year":"2002","journal-title":"Hear. Res."},{"key":"10.1016\/j.heares.2016.02.019_bib11","doi-asserted-by":"crossref","first-page":"9363","DOI":"10.1073\/pnas.92.20.9363","article-title":"A\u00a0biomarker that identifies senescent human cells in culture and in aging skin in\u00a0vivo","volume":"92","author":"Dimri","year":"1995","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.heares.2016.02.019_bib12","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1002\/jnr.490240226","article-title":"Aminoglycoside antibiotics impair calcium entry but not viability and motility in isolated cochlear outer hair cells","volume":"24","author":"Dulon","year":"1989","journal-title":"J.\u00a0Neurosci. Res."},{"key":"10.1016\/j.heares.2016.02.019_bib13","doi-asserted-by":"crossref","first-page":"1907","DOI":"10.1128\/IAI.73.4.1907-1916.2005","article-title":"Apoptosis, pyroptosis, and necrosis: mechanistic description of dead and dying eukaryotic cells","volume":"73","author":"Fink","year":"2005","journal-title":"Infect. Immun."},{"key":"10.1016\/j.heares.2016.02.019_bib14","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1038\/nrm3999","article-title":"Live to die another way: modes of programmed cell death and the signals emanating from dying cells","volume":"16","author":"Fuchs","year":"2015","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.heares.2016.02.019_bib15","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1161\/CIRCRESAHA.116.303787","article-title":"Untangling autophagy measurements: all fluxed up","volume":"116","author":"Gottlieb","year":"2015","journal-title":"Circ. Res."},{"key":"10.1016\/j.heares.2016.02.019_bib16","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.cellsig.2014.11.024","article-title":"The autophagy pathway maintained signaling crosstalk with the Keap1-Nrf2 system through p62 in auditory cells under oxidative stress","volume":"27","author":"Hayashi","year":"2015","journal-title":"Cell. Signal."},{"key":"10.1016\/j.heares.2016.02.019_bib17","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/0014-4827(65)90211-9","article-title":"The limited in\u00a0vitro lifetime of human diploid cell strains","volume":"37","author":"Hayflick","year":"1965","journal-title":"Exp. Cell Res."},{"key":"10.1016\/j.heares.2016.02.019_bib18","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/S0022-3565(25)21759-1","article-title":"Effect of aminoglycoside antibiotics on cellular functions of kidney epithelial cell line (LLC-PK1): a model system for aminoglycoside nephrotoxicity","volume":"230","author":"Hori","year":"1984","journal-title":"J.\u00a0Pharmacol. Exp. Ther."},{"key":"10.1016\/j.heares.2016.02.019_bib19","doi-asserted-by":"crossref","first-page":"R11","DOI":"10.1016\/0006-2952(90)90077-X","article-title":"Formation of a cytotoxic metabolite from gentamicin by liver","volume":"40","author":"Huang","year":"1990","journal-title":"Biochem. Pharmacol."},{"key":"10.1016\/j.heares.2016.02.019_bib20","doi-asserted-by":"crossref","first-page":"5096","DOI":"10.1073\/pnas.88.12.5096","article-title":"Direct derivation of conditionally immortal cell lines from an H-2Kb-tsA58 transgenic mouse","volume":"88","author":"Jat","year":"1991","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.heares.2016.02.019_bib21","doi-asserted-by":"crossref","first-page":"180","DOI":"10.3389\/fnins.2010.00180","article-title":"The new inhibitor of monoamine oxidase, M30, has a neuroprotective effect against dexamethasone-induced brain cell apoptosis","volume":"4","author":"Johnson","year":"2010","journal-title":"Front. Neurosci."},{"key":"10.1016\/j.heares.2016.02.019_bib22","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1159\/000071059","article-title":"A\u00a0cochlear cell line as an in\u00a0vitro system for drug ototoxicity screening","volume":"8","author":"Kalinec","year":"2003","journal-title":"Audiol. Neurootol."},{"key":"10.1016\/j.heares.2016.02.019_bib23","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.heares.2014.04.007","article-title":"Acetaminophen and NAPQI are toxic to auditory cells via oxidative and endoplasmic reticulum stress-dependent pathways","volume":"313","author":"Kalinec","year":"2014","journal-title":"Hear. Res."},{"key":"10.1016\/j.heares.2016.02.019_bib24","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1248\/bpb.29.281","article-title":"Effects of dexamethasone and aminophylline on survival of Jurkat and HL-60 cells","volume":"29","author":"Kizawa","year":"2006","journal-title":"Biol. Pharm. Bull."},{"key":"10.1016\/j.heares.2016.02.019_bib25","doi-asserted-by":"crossref","first-page":"151","DOI":"10.4161\/auto.5338","article-title":"Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes","volume":"4","author":"Klionsky","year":"2008","journal-title":"Autophagy"},{"key":"10.1016\/j.heares.2016.02.019_bib26","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.cell.2004.11.046","article-title":"Growth factor regulation of autophagy and cell survival in the absence of apoptosis","volume":"120","author":"Lum","year":"2005","journal-title":"Cell"},{"key":"10.1016\/j.heares.2016.02.019_bib27","first-page":"3","article-title":"Apoptosis, oncosis, and necrosis. An overview of cell death","volume":"146","author":"Majno","year":"1995","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.heares.2016.02.019_bib28","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1038\/nrm3735","article-title":"Self-consumption: the interplay of autophagy and apoptosis","volume":"15","author":"Marino","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.heares.2016.02.019_bib29","doi-asserted-by":"crossref","first-page":"821","DOI":"10.18632\/aging.100380","article-title":"Hormesis, cell death and aging","volume":"3","author":"Martins","year":"2011","journal-title":"Aging (Albany NY)"},{"key":"10.1016\/j.heares.2016.02.019_bib30","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1288\/00005537-197204000-00003","article-title":"Cochlear microcirculation and oxygen transport","volume":"82","author":"Mayahara","year":"1972","journal-title":"Laryngoscope"},{"key":"10.1016\/j.heares.2016.02.019_bib31","doi-asserted-by":"crossref","first-page":"542","DOI":"10.4161\/auto.4600","article-title":"How to interpret LC3 immunoblotting","volume":"3","author":"Mizushima","year":"2007","journal-title":"Autophagy"},{"key":"10.1016\/j.heares.2016.02.019_bib32","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.cell.2010.01.028","article-title":"Methods in mammalian autophagy research","volume":"140","author":"Mizushima","year":"2010","journal-title":"Cell"},{"key":"10.1016\/j.heares.2016.02.019_bib33","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","article-title":"Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays","volume":"65","author":"Mosmann","year":"1983","journal-title":"J.\u00a0Immunol. Methods"},{"key":"10.1016\/j.heares.2016.02.019_bib34","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.ctrv.2006.09.006","article-title":"Molecular mechanisms of resistance and toxicity associated with platinating agents","volume":"33","author":"Rabik","year":"2007","journal-title":"Cancer Treat Rev."},{"key":"10.1016\/j.heares.2016.02.019_bib35","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1083\/jcb.201009094","article-title":"Four faces of cellular senescence","volume":"192","author":"Rodier","year":"2011","journal-title":"J.\u00a0Cell Biol."},{"key":"10.1016\/j.heares.2016.02.019_bib36","first-page":"229","article-title":"Death by any other name","volume":"147","author":"Schwartz","year":"1995","journal-title":"Am. J. Pathol."},{"key":"10.1016\/j.heares.2016.02.019_bib37","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1002\/bies.201500150","article-title":"With or without rafts? Alternative views on cell membranes","volume":"38","author":"Sevcsik","year":"2016","journal-title":"Bioessays"},{"key":"10.1016\/j.heares.2016.02.019_bib38","doi-asserted-by":"crossref","first-page":"1221","DOI":"10.1038\/ncb1192","article-title":"Role of Bcl-2 family proteins in a non-apoptotic programmed cell death dependent on autophagy genes","volume":"6","author":"Shimizu","year":"2004","journal-title":"Nat. Cell Biol."},{"key":"10.1016\/j.heares.2016.02.019_bib50","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1093\/molehr\/gal081","article-title":"What is the appropriate oxygen tension for in vitro culture?","volume":"12","author":"Sullivan","year":"2006","journal-title":"Mol. Hum. Reprod."},{"key":"10.1016\/j.heares.2016.02.019_bib39","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/j.tibs.2014.10.003","article-title":"A\u00a0new kind of cell suicide: mechanisms and functions of programmed necrosis","volume":"39","author":"Sun","year":"2014","journal-title":"Trends Biochem. Sci."},{"key":"10.1016\/j.heares.2016.02.019_bib40","doi-asserted-by":"crossref","first-page":"2240","DOI":"10.1091\/mbc.e11-11-0926","article-title":"Cell survival, DNA damage, and oncogenic transformation after a transient and reversible apoptotic response","volume":"23","author":"Tang","year":"2012","journal-title":"Mol. Biol. Cell"},{"key":"10.1016\/j.heares.2016.02.019_bib41","doi-asserted-by":"crossref","first-page":"3644","DOI":"10.18632\/oncotarget.2874","article-title":"Autophagy through 4EBP1 and AMPK regulates oxidative stress-induced premature senescence in auditory cells","volume":"6","author":"Tsuchihashi","year":"2015","journal-title":"Oncotarget"},{"key":"10.1016\/j.heares.2016.02.019_bib42","doi-asserted-by":"crossref","first-page":"437","DOI":"10.3109\/00016486509124578","article-title":"Cochlear oxygen tension: relation to blood flow and function","volume":"59","author":"Tsunoo","year":"1965","journal-title":"Acta Otolaryngol."},{"key":"10.1016\/j.heares.2016.02.019_bib43","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/nrm3737","article-title":"Regulated necrosis: the expanding network of non-apoptotic cell death pathways","volume":"15","author":"Vanden Berghe","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.heares.2016.02.019_bib44","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1084\/jem.188.5.919","article-title":"Dual signaling of the Fas receptor: initiation of both apoptotic and necrotic cell death pathways","volume":"188","author":"Vercammen","year":"1998","journal-title":"J.\u00a0Exp. Med."},{"key":"10.1016\/j.heares.2016.02.019_bib45","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1016\/j.ijporl.2015.08.012","article-title":"Role of autophagy in cisplatin-induced ototoxicity","volume":"79","author":"Youn","year":"2015","journal-title":"Int. J. Pediatr. Otorhinolaryngol."},{"key":"10.1016\/j.heares.2016.02.019_bib46","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1038\/344126a0","article-title":"In\u00a0vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAs","volume":"344","author":"Yu","year":"1990","journal-title":"Nature"},{"key":"10.1016\/j.heares.2016.02.019_bib47","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1002\/ana.21812","article-title":"Depletion of the neural precursor cell pool by glucocorticoids","volume":"67","author":"Yu","year":"2010","journal-title":"Ann. Neurol."},{"key":"10.1016\/j.heares.2016.02.019_bib48","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1007\/BF00179898","article-title":"Specific glutathione-SH inhibition of toxic effects of metabolized gentamicin on isolated guinea pig hair cells","volume":"251","author":"Zenner","year":"1994","journal-title":"Eur. Arch. Otorhinolaryngol."},{"key":"10.1016\/j.heares.2016.02.019_bib49","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1101\/gad.1376506","article-title":"Necrotic death as a cell fate","volume":"20","author":"Zong","year":"2006","journal-title":"Genes Dev."}],"container-title":["Hearing Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S037859551530188X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S037859551530188X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T11:16:17Z","timestamp":1748776577000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S037859551530188X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5]]},"references-count":50,"alternative-id":["S037859551530188X"],"URL":"https:\/\/doi.org\/10.1016\/j.heares.2016.02.019","relation":{},"ISSN":["0378-5955"],"issn-type":[{"value":"0378-5955","type":"print"}],"subject":[],"published":{"date-parts":[[2016,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"HEI-OC1 cells as a model for investigating drug cytotoxicity","name":"articletitle","label":"Article Title"},{"value":"Hearing Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.heares.2016.02.019","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}