{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T15:10:50Z","timestamp":1785337850011,"version":"3.55.0"},"reference-count":31,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Hearing Research"],"published-print":{"date-parts":[[2026,9]]},"DOI":"10.1016\/j.heares.2026.109748","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T06:23:32Z","timestamp":1784010212000},"page":"109748","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["An optimized tissue processing pipeline for multiscale imaging of the intact peripheral auditory pathway"],"prefix":"10.1016","volume":"479","author":[{"given":"Jan Frederik","family":"Ahrend","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louisa S.","family":"Kuenkel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald","family":"Degenhart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4432-2733","authenticated-orcid":false,"given":"Christian","family":"Vogl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.heares.2026.109748_bib0001","doi-asserted-by":"crossref","first-page":"4496","DOI":"10.1073\/pnas.1817537116","article-title":"Dual AAV-mediated gene therapy restores hearing in a DFNB9 mouse model","volume":"116","author":"Akil","year":"2019","journal-title":"Proc. Natl. Acad. Sci"},{"key":"10.1016\/j.heares.2026.109748_bib23","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0304-3940(98)00241-9","article-title":"Unbiased number of vestibular ganglion neurons in the mouse","volume":"246","author":"B\u00e4urle","year":"1998","journal-title":"Neurosci. Lett."},{"key":"10.1016\/j.heares.2026.109748_bib24","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202517738","article-title":"Skoots: Skeleton-oriented object segmentation for mitochondria in high-resolution cochlear em datasets","volume":"13","author":"Buswinka","year":"2026","journal-title":"Adv. Sci."},{"key":"10.1016\/j.heares.2026.109748_bib0003","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2025.112929","article-title":"Spiner, deep learning-based automated detection of spiral ganglion neurons in intact cochleae","volume":"28","author":"Cai","year":"2025","journal-title":"iScience"},{"key":"10.1016\/j.heares.2026.109748_bib0004","doi-asserted-by":"crossref","DOI":"10.7717\/peerj.19512","article-title":"Volumetric atlas of the rat inner ear from microCT and iDISCO+ cleared temporal bones","volume":"13","author":"Cossellu","year":"2025","journal-title":"PeerJ"},{"key":"10.1016\/j.heares.2026.109748_bib0005","article-title":"Lightsheet microscopy","volume":"2","author":"Dyer","year":"2022","journal-title":"Curr. Protoc."},{"key":"10.1016\/j.heares.2026.109748_bib0006","doi-asserted-by":"crossref","first-page":"2718","DOI":"10.1016\/j.celrep.2016.02.057","article-title":"Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent","volume":"14","author":"Ke","year":"2016","journal-title":"Cell Rep."},{"key":"10.1016\/j.heares.2026.109748_bib0007","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2014472118","article-title":"Multiscale photonic imaging of the native and implanted cochlea","volume":"118","author":"Keppeler","year":"2021","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.heares.2026.109748_bib25","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1681\/ASN.2016020232","article-title":"Fully automated evaluation of total glomerular number and capillary tuft size in nephritic kidneys using lightsheet microscopy","volume":"28","author":"Klingberg","year":"2017","journal-title":"J. Am. Soc. Nephrol. JASN"},{"key":"10.1016\/j.heares.2026.109748_bib26","doi-asserted-by":"crossref","first-page":"eadf7084","DOI":"10.1126\/sciadv.adf7084","article-title":"Glyoxal fixation: An approach to solve immunohistochemical problem in neuroscience research","volume":"9","author":"Konno","year":"2023","journal-title":"Sci. Adv."},{"key":"10.1016\/j.heares.2026.109748_bib27","first-page":"1","article-title":"n.d. The segregation of Calb1, Calb2, and Prph neurons reveals distinct and mixed neuronal populations and projections to hair cells in the inner ear and central nuclei","author":"Lee","year":"2025","journal-title":"Dev. Dyn."},{"key":"10.1016\/j.heares.2026.109748_bib0009","article-title":"Broad applicability of a streamlined ethyl cinnamate-based clearing procedure","volume":"146","author":"Masselink","year":"2019","journal-title":"Development"},{"key":"10.1016\/j.heares.2026.109748_bib28","doi-asserted-by":"crossref","first-page":"6181","DOI":"10.1364\/BOE.402991","article-title":"Three-dimensional imaging of intact porcine cochlea using tissue clearing and custom-built light-sheet microscopy","volume":"11","author":"Moatti","year":"2020","journal-title":"Biomed. Opt. Express"},{"key":"10.1016\/j.heares.2026.109748_bib0010","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2022.104695","article-title":"Ontogeny of cellular organization and LGR5 expression in porcine cochlea revealed using tissue clearing and 3D imaging","volume":"25","author":"Moatti","year":"2022","journal-title":"iScience"},{"key":"10.1016\/j.heares.2026.109748_bib0011","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1038\/s41467-018-08264-w","article-title":"Improved TMC1 gene therapy restores hearing and balance in mice with genetic inner ear disorders","volume":"10","author":"Nist-Lund","year":"2019","journal-title":"Nat. Commun."},{"key":"10.1016\/j.heares.2026.109748_bib0012","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1038\/nbt.3801","article-title":"Gene therapy restores auditory and vestibular function in a mouse model of Usher syndrome type 1c","volume":"35","author":"Pan","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.heares.2026.109748_bib0013","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s10162-025-01024-w","article-title":"Regulatory networks driving the specification, differentiation, and diversification of neurons in the mouse inner ear","volume":"27","author":"Pavlinkova","year":"2026","journal-title":"JARO J. Assoc. Res. Otolaryngol."},{"key":"10.1016\/j.heares.2026.109748_bib0014","doi-asserted-by":"crossref","first-page":"15","DOI":"10.3389\/fnana.2019.00015","article-title":"iDISCO+ for the study of neuroimmune architecture of the Rat auditory brainstem","volume":"13","author":"Perin","year":"2019","journal-title":"Front. Neuroanat."},{"key":"10.1016\/j.heares.2026.109748_bib0015","doi-asserted-by":"crossref","DOI":"10.3389\/fnmol.2020.600051","article-title":"Overloaded adeno-associated virus as a novel gene therapeutic tool for otoferlin-related deafness","volume":"13","author":"Rankovic","year":"2021","journal-title":"Front. Mol. Neurosci"},{"key":"10.1016\/j.heares.2026.109748_bib0017","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1016\/j.cell.2014.10.010","article-title":"iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging","volume":"159","author":"Renier","year":"2014","journal-title":"Cell"},{"key":"10.1016\/j.heares.2026.109748_bib0016","doi-asserted-by":"crossref","first-page":"1789","DOI":"10.1016\/j.cell.2016.05.007","article-title":"Mapping of brain activity by automated volume analysis of immediate early genes","volume":"165","author":"Renier","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.heares.2026.109748_bib29","doi-asserted-by":"crossref","first-page":"139","DOI":"10.15252\/embj.201695709","article-title":"Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy","volume":"37","author":"Richter","year":"2018","journal-title":"EMBO J"},{"key":"10.1016\/j.heares.2026.109748_bib0018","article-title":"CochleaNet: deep learning-based image analysis for cochlear connectomics and gene therapy","author":"Roos","year":"2025","journal-title":"bioRxiv"},{"key":"10.1016\/j.heares.2026.109748_bib0019","doi-asserted-by":"crossref","DOI":"10.1016\/j.cell.2018.07.007","article-title":"Sensory neuron diversity in the inner ear is shaped by activity","volume":"174","author":"Shrestha","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.heares.2026.109748_bib0020","doi-asserted-by":"crossref","DOI":"10.1016\/j.cell.2018.07.008","article-title":"Hair cell mechanotransduction regulates spontaneous activity and spiral ganglion subtype specification in the auditory system","volume":"174","author":"Sun","year":"2018","journal-title":"Cell"},{"key":"10.1016\/j.heares.2026.109748_bib0021","doi-asserted-by":"crossref","DOI":"10.1016\/j.celrep.2018.07.056","article-title":"Chemical landscape for tissue clearing based on hydrophilic reagents","volume":"24","author":"Tainaka","year":"2018","journal-title":"Cell Rep."},{"key":"10.1016\/j.heares.2026.109748_bib30","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1038\/s41583-019-0250-1","article-title":"Tissue clearing and its applications in neuroscience","volume":"21","author":"Ueda","year":"2020","journal-title":"Nat. Rev. Neurosci."},{"key":"10.1016\/j.heares.2026.109748_bib31","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.40946","article-title":"Cellular cartography of the organ of Corti based on optical tissue clearing and machine learning","volume":"8","author":"Urata","year":"2019","journal-title":"eLife"},{"key":"10.1016\/j.heares.2026.109748_bib0022","doi-asserted-by":"crossref","DOI":"10.1016\/j.mcn.2022.103720","article-title":"Methods for multiscale structural and functional analysis of the mammalian cochlea","volume":"120","author":"Vogl","year":"2022","journal-title":"Mol. Cell. Neurosci."},{"key":"10.1016\/j.heares.2026.109748_bib32","doi-asserted-by":"crossref","first-page":"5709","DOI":"10.1523\/JNEUROSCI.0830-19.2020","article-title":"Accumulation of neurofascin at nodes of Ranvier is regulated by a paranodal switch","volume":"40","author":"Zhang","year":"2020","journal-title":"J. Neurosci."},{"key":"10.1016\/j.heares.2026.109748_bib33","doi-asserted-by":"crossref","first-page":"11480","DOI":"10.1073\/pnas.1819583116","article-title":"Ultrafast optical clearing method for three-dimensional imaging with cellular resolution","volume":"116","author":"Zhu","year":"2019","journal-title":"Proc. Natl. Acad. Sci."}],"container-title":["Hearing Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378595526002194?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378595526002194?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T14:43:42Z","timestamp":1785336222000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378595526002194"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":31,"alternative-id":["S0378595526002194"],"URL":"https:\/\/doi.org\/10.1016\/j.heares.2026.109748","relation":{},"ISSN":["0378-5955"],"issn-type":[{"value":"0378-5955","type":"print"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An optimized tissue processing pipeline for multiscale imaging of the intact peripheral auditory pathway","name":"articletitle","label":"Article Title"},{"value":"Hearing Research","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.heares.2026.109748","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier B.V.","name":"copyright","label":"Copyright"}],"article-number":"109748"}}