{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T07:59:43Z","timestamp":1769241583592,"version":"3.49.0"},"reference-count":19,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T00:00:00Z","timestamp":1517443200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2018,2,19]],"date-time":"2018-02-19T00:00:00Z","timestamp":1518998400000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Heliyon"],"published-print":{"date-parts":[[2018,2]]},"DOI":"10.1016\/j.heliyon.2018.e00549","type":"journal-article","created":{"date-parts":[[2018,2,28]],"date-time":"2018-02-28T23:11:16Z","timestamp":1519859476000},"page":"e00549","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"title":["Development of single step RT-PCR for detection of Kyasanur forest disease virus from clinical samples"],"prefix":"10.1016","volume":"4","author":[{"given":"Gouri","family":"Chaubal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prasad","family":"Sarkale","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pravin","family":"Kore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pragya","family":"Yadav","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.heliyon.2018.e00549_bib1","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s40249-015-0044-2","article-title":"New focus of Kyasanur Forest disease virus activity in a tribal area in Kerala, India, in 2014","volume":"4","author":"Tandale","year":"2015","journal-title":"Infect. Dis. Poverty"},{"issue":"9","key":"10.1016\/j.heliyon.2018.e00549_bib2","doi-asserted-by":"crossref","DOI":"10.3201\/eid1909.121884","article-title":"Spread of Kyasanur forest disease, Bandipur tiger Reserve, India, 2012\u20132013","volume":"19","author":"Mourya","year":"2013","journal-title":"Emerg. Infect. Dis."},{"issue":"6","key":"10.1016\/j.heliyon.2018.e00549_bib3","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/j.jinf.2016.03.006","article-title":"Outbreak of Kyasanur forest disease (monkey fever) in Sindhudurg, Maharashtra state, India","volume":"72","author":"Awate","year":"2016","journal-title":"J. Infect."},{"key":"10.1016\/j.heliyon.2018.e00549_bib4","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s40249-015-0066-9","article-title":"On the transmission pattern of Kyasanur Forest disease (KFD) in India","volume":"4","author":"Murhekar","year":"2015","journal-title":"Infect. Dis. Poverty"},{"issue":"1","key":"10.1016\/j.heliyon.2018.e00549_bib5","doi-asserted-by":"crossref","first-page":"8","DOI":"10.4103\/2224-3151.206890","article-title":"Highly infectious tick borne viral diseases: Kyasanur forest disease and Crimean\u2013Congo hemorrhagic fever in India","volume":"3","author":"Mourya","year":"2014","journal-title":"WHO South\u2013East Asia J. Public Health"},{"key":"10.1016\/j.heliyon.2018.e00549_bib6","unstructured":"Department of Information & Technology, Government of Goa. Fever Cases at Pali, Confirmed as Kyasanur Forest Disease. Available at: https:\/\/www.goa.gov.in\/pdf\/FEVER%20CASES%20AT%20PALI.pdf."},{"key":"10.1016\/j.heliyon.2018.e00549_bib7","doi-asserted-by":"crossref","first-page":"S854","DOI":"10.1093\/clinids\/11.Supplement_4.S854","article-title":"Clinical, clinicopathologic, and hematologic features of Kyasanur forest disease","volume":"11","author":"Pavri","year":"1989","journal-title":"Rev. Infect. Dis."},{"key":"10.1016\/j.heliyon.2018.e00549_bib8","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1002\/rmv.495","article-title":"Kyasanur forest disease: an epidemiological view in India","volume":"16","author":"Pattnaik","year":"2006","journal-title":"Rev. Med. Virol."},{"key":"10.1016\/j.heliyon.2018.e00549_bib9","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.antiviral.2012.10.005","article-title":"Kyasanur forest disease","volume":"96","author":"Holbrook","year":"2012","journal-title":"Antivir. Res."},{"key":"10.1016\/j.heliyon.2018.e00549_bib10","first-page":"340","article-title":"Summary of preliminary report of investigations of the virus research centre on an epidemic disease affecting forest villagers and wild monkeys in Shimoga district, Mysore","volume":"11","author":"Work","year":"1957","journal-title":"Indian J. Med. Sci."},{"key":"10.1016\/j.heliyon.2018.e00549_bib11","series-title":"Arboviruses Epidemiology and Ecology. Boca Raton (FL)","first-page":"93","article-title":"Kyasanur forest disease","author":"Banerjee","year":"1988"},{"key":"10.1016\/j.heliyon.2018.e00549_bib12","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.jviromet.2012.07.019","article-title":"Diagnosis of Kyasanur forest disease by nested RT-PCR, real-time RT-PCR and IgM Capture ELISA","volume":"186","author":"Mourya","year":"2012","journal-title":"J. Virol. Method"},{"issue":"9","key":"10.1016\/j.heliyon.2018.e00549_bib13","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.3201\/eid1509.080759","article-title":"Recent ancestry of Kyasanur forest disease virus","volume":"15","author":"Mehla","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.heliyon.2018.e00549_bib14","series-title":"Virus Taxonomy\u2014Eighth Report of the International Committee on Taxonomy of Viruses","first-page":"981","article-title":"Flaviviridae","author":"Thiel","year":"2005"},{"key":"10.1016\/j.heliyon.2018.e00549_bib15","doi-asserted-by":"crossref","first-page":"326","DOI":"10.3201\/eid1502.080979","article-title":"Isolation of Kyasanur forest disease virus from febrile patient, Yunnan, China","volume":"15","author":"Wang","year":"2009","journal-title":"Emerg. Infect. Dis."},{"issue":"2","key":"10.1016\/j.heliyon.2018.e00549_bib16","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.jviromet.2004.03.015","article-title":"Virus inactivation by nucleic acid extraction reagents","volume":"119","author":"Blow","year":"2004","journal-title":"J. Virol. Methods"},{"key":"10.1016\/j.heliyon.2018.e00549_bib17","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0035-9203(97)90215-7","article-title":"Isolation of a flavivirus related to the tick-borne encephalitis complex from human cases in Saudi Arabia","volume":"91","author":"Zaki","year":"1997","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.heliyon.2018.e00549_bib18","first-page":"1","article-title":"A novel flavivirus: Makkah Region, 1994\u20131996","volume":"3","author":"Qattan","year":"1996","journal-title":"Saudi Epidemiol. Bull."},{"key":"10.1016\/j.heliyon.2018.e00549_bib19","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1006\/bbrc.2001.5610","article-title":"Complete coding sequence of the Alkhurma virus, a tick-borne flavivirus causing severe hemorrhagic fever in humans in Saudi Arabia","volume":"287","author":"Charrel","year":"2001","journal-title":"Biochem. Biophys. Res. Commun."}],"container-title":["Heliyon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405844018300045?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405844018300045?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T10:22:10Z","timestamp":1762510930000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405844018300045"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2]]},"references-count":19,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["S2405844018300045"],"URL":"https:\/\/doi.org\/10.1016\/j.heliyon.2018.e00549","relation":{},"ISSN":["2405-8440"],"issn-type":[{"value":"2405-8440","type":"print"}],"subject":[],"published":{"date-parts":[[2018,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development of single step RT-PCR for detection of Kyasanur forest disease virus from clinical samples","name":"articletitle","label":"Article Title"},{"value":"Heliyon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.heliyon.2018.e00549","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"e00549"}}