{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T05:25:00Z","timestamp":1791955500112,"version":"4.3.4"},"reference-count":44,"publisher":"Elsevier BV","issue":"8","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T00:00:00Z","timestamp":1597276800000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006143","name":"Department of Science and Technology, Government of Kerala","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006143","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007741","name":"University of Hyderabad","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007741","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","award":["SR\/WOS-A\/LS-294\/2012(G)"],"award-info":[{"award-number":["SR\/WOS-A\/LS-294\/2012(G)"]}],"id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001501","name":"University Grants Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Heliyon"],"published-print":{"date-parts":[[2020,8]]},"DOI":"10.1016\/j.heliyon.2020.e04734","type":"journal-article","created":{"date-parts":[[2020,8,19]],"date-time":"2020-08-19T09:29:37Z","timestamp":1597829377000},"page":"e04734","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":91,"title":["Functional and molecular characterization of plant growth promoting Bacillus isolates from tomato rhizosphere"],"prefix":"10.1016","volume":"6","author":[{"given":"Sadaf","family":"Kalam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6620-8320","authenticated-orcid":false,"given":"Anirban","family":"Basu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3208-765X","authenticated-orcid":false,"given":"Appa Rao","family":"Podile","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.heliyon.2020.e04734_bib1","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.micres.2006.04.001","article-title":"Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities","volume":"163","author":"Ahmad","year":"2008","journal-title":"Microbiol. Res."},{"key":"10.1016\/j.heliyon.2020.e04734_bib2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2018\/5686874","article-title":"Evaluation of Bacillus strains for plant growth promotion and predictability of efficacy by in vitro physiological traits","volume":"2018","author":"Akinrinlola","year":"2018","journal-title":"Int. J. Microbiol."},{"key":"10.1016\/j.heliyon.2020.e04734_bib3","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.3389\/fpls.2018.01473","article-title":"Plant growth-promoting rhizobacteria: context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture","volume":"9","author":"Backer","year":"2018","journal-title":"Front. Plant Sci."},{"key":"10.1016\/j.heliyon.2020.e04734_bib4","series-title":"Principles of Plant-Microbe Interactions","first-page":"225","article-title":"Phosphate mobilisation by soil microorganisms","author":"Barea","year":"2015"},{"key":"10.1016\/j.heliyon.2020.e04734_bib5","doi-asserted-by":"crossref","first-page":"E1621","DOI":"10.1073\/pnas.1218984110","article-title":"Bacillus subtilis biofilm induction by plant polysaccharides","volume":"110","author":"Beauregard","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.heliyon.2020.e04734_bib6","doi-asserted-by":"crossref","first-page":"4360","DOI":"10.1038\/s41598-018-22782-z","article-title":"Antagonism of two plant-growth promoting Bacillus velezensis isolates against Ralstonia solanacearum and Fusarium oxysporum","volume":"8","author":"Cao","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.heliyon.2020.e04734_bib7","series-title":"Microbiology: a Laboratory Manual","author":"Cappuccino","year":"2014"},{"key":"10.1016\/j.heliyon.2020.e04734_bib8","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1111\/j.1462-2920.2012.02860.x","article-title":"Biocontrol of tomato wilt disease by Bacillus subtilis isolates from natural environments depends on conserved genes mediating biofilm formation","volume":"15","author":"Chen","year":"2013","journal-title":"Environ. Microbiol."},{"issue":"4","key":"10.1016\/j.heliyon.2020.e04734_bib9","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1007\/s00344-010-9152-1","article-title":"Plant growth-promoting chitinolytic Paenibacillus elgii responds positively to tobacco root exudates","volume":"29","author":"Das","year":"2010","journal-title":"J.\u00a0Plant Growth Regul."},{"issue":"2","key":"10.1016\/j.heliyon.2020.e04734_bib10","doi-asserted-by":"crossref","first-page":"206","DOI":"10.5505\/tjb.2014.26817","article-title":"Screening of phytate hydrolysis Bacillus sp. isolated from soil and optimization of the certain nutritional and physical parameters on the production of phytase","volume":"39","author":"Demirkan","year":"2014","journal-title":"Turk. J. Bioch."},{"issue":"3","key":"10.1016\/j.heliyon.2020.e04734_bib11","doi-asserted-by":"crossref","first-page":"232","DOI":"10.3109\/10408411003766806","article-title":"Plant Growth Promoting Rhizobacteria (PGPR): the bugs to debug the root zone","volume":"36","author":"Dutta","year":"2010","journal-title":"Crit. Rev. Microbiol."},{"key":"10.1016\/j.heliyon.2020.e04734_bib12","series-title":"Bacterial Metabolites in Sustainable Agroecosystem","first-page":"183","article-title":"Indole-3-acetic acid and 1-aminocyclopropane-1-carboxylate deaminase: bacterial traits required in rhizosphere, rhizoplane and\/or endophytic competence by beneficial bacteria","author":"Etesami","year":"2015"},{"key":"10.1016\/j.heliyon.2020.e04734_bib13","series-title":"Biofilms in Plant and Soil Health","first-page":"337","article-title":"Plant root-associated biofilms in bioremediation","author":"Kalam","year":"2017"},{"issue":"5","key":"10.1016\/j.heliyon.2020.e04734_bib14","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1002\/jobm.201600588","article-title":"Population densities of indigenous Acidobacteria change in the presence of plant growth promoting rhizobacteria (PGPR) in rhizosphere","volume":"57","author":"Kalam","year":"2017","journal-title":"J.\u00a0Basic Microbiol."},{"key":"10.1016\/j.heliyon.2020.e04734_bib15","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1111\/j.1472-765X.2005.01664.x","article-title":"Phylloplane bacteria increase seedling emergence, growth and yield of field-grown groundnut (Arachis hypogaea L.)","volume":"40","author":"Kishore","year":"2005","journal-title":"Lett. Appl. Microbiol."},{"issue":"9","key":"10.1016\/j.heliyon.2020.e04734_bib16","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0024452","article-title":"Desert farming benefits from microbial potential in arid soils and promotes diversity and plant health","volume":"6","author":"K\u00f6berl","year":"2011","journal-title":"PloS One"},{"issue":"8","key":"10.1016\/j.heliyon.2020.e04734_bib17","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.micres.2012.05.002","article-title":"Bacillus strains isolated from rhizosphere showed plant growth promoting and antagonistic activity against phytopathogens","volume":"167","author":"Kumar","year":"2012","journal-title":"Microbiol. Res."},{"issue":"8","key":"10.1016\/j.heliyon.2020.e04734_bib18","doi-asserted-by":"crossref","first-page":"1361","DOI":"10.1007\/s11274-013-1299-z","article-title":"Isolation of phytase-producing bacteria from Himalayan soils and their effect on growth and phosphorus uptake of Indian mustard (Brassica juncea)","volume":"29","author":"Kumar","year":"2013","journal-title":"World J. Microbiol. Biotechnol."},{"key":"10.1016\/j.heliyon.2020.e04734_bib19","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/1471-2180-7-58","article-title":"Analysis of Chromobacterium sp. natural isolates from different Brazilian ecosystems","volume":"7","author":"Lima-Bittencourt","year":"2007","journal-title":"BMC Microbiol."},{"issue":"4","key":"10.1016\/j.heliyon.2020.e04734_bib20","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0152951","article-title":"Bioprospecting of plant growth promoting Bacilli and related genera prevalent in soils of pristine sacred groves: biochemical and molecular approach","volume":"11","author":"Lyngwi","year":"2016","journal-title":"PloS One"},{"issue":"1","key":"10.1016\/j.heliyon.2020.e04734_bib21","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1590\/S1415-47572008000100019","article-title":"BOX-PCR-based identification of bacterial species belonging to Pseudomonas syringae: P. viridiflava group","volume":"31","author":"Marques","year":"2008","journal-title":"Genet. Mol. Biol."},{"key":"10.1016\/j.heliyon.2020.e04734_bib22","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.micres.2017.06.001","article-title":"Zinc solubilizing Bacillus spp. potential candidates for biofortification in maize","volume":"202","author":"Mumtaz","year":"2017","journal-title":"Microbiol. Res."},{"key":"10.1016\/j.heliyon.2020.e04734_bib23","doi-asserted-by":"crossref","first-page":"126439","DOI":"10.1016\/j.micres.2020.126439","article-title":"ACC deaminase in plant growth-promoting bacteria (PGPB): an efficient mechanism to counter salt stress in crops","volume":"235","author":"Orozco-Mosqueda","year":"2020","journal-title":"Microbiol. Res."},{"issue":"3","key":"10.1016\/j.heliyon.2020.e04734_bib24","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1007\/s00344-016-9583-4","article-title":"Current perspectives on plant growth-promoting rhizobacteria","volume":"35","author":"Parray","year":"2016","journal-title":"J.\u00a0Plant Growth Regul."},{"key":"10.1016\/j.heliyon.2020.e04734_bib25","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1186\/s12866-017-1039-x","article-title":"Addition of plant-growth-promoting Bacillus subtilis PTS-394 on tomato rhizosphere has no durable impact on composition of root microbiome","volume":"17","author":"Qiao","year":"2017","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.heliyon.2020.e04734_bib26","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1007\/s13213-011-0211-z","article-title":"Identification of multi-trait PGPR isolates and evaluating their potential as inoculants for wheat","volume":"61","author":"Rana","year":"2011","journal-title":"Ann. Microbiol."},{"key":"10.1016\/j.heliyon.2020.e04734_bib27","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.3389\/fmicb.2016.01785","article-title":"Hydrogen cyanide in the rhizosphere: not suppressing plant pathogens, but rather regulating availability of phosphate","volume":"7","author":"Rijavec","year":"2016","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.heliyon.2020.e04734_bib28","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.scienta.2015.08.042","article-title":"Plant growth-promoting rhizobacteria act as biostimulants in horticulture","volume":"196","author":"Ruzzi","year":"2015","journal-title":"Sci. Hortic."},{"issue":"8","key":"10.1016\/j.heliyon.2020.e04734_bib29","doi-asserted-by":"crossref","first-page":"4927","DOI":"10.1073\/pnas.0730845100","article-title":"Bacterial volatiles promote growth in Arabidopsis","volume":"100","author":"Ryu","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"10.1016\/j.heliyon.2020.e04734_bib30","doi-asserted-by":"crossref","first-page":"3984","DOI":"10.1007\/s11356-015-4294-0","article-title":"Microbial siderophores and their potential applications: a review","volume":"23","author":"Saha","year":"2016","journal-title":"Environ. Sci. Pollut. Res."},{"key":"10.1016\/j.heliyon.2020.e04734_bib31","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/s11104-015-2512-7","article-title":"Assessment of functional diversity and structure of phytate-hydrolysing bacterial community in Lolium perenne rhizosphere","volume":"401","author":"Sanguin","year":"2016","journal-title":"Plant Soil"},{"key":"10.1016\/j.heliyon.2020.e04734_bib32","series-title":"Secondary Metabolites of Plant Growth Promoting Rhizomicroorganisms","first-page":"225","article-title":"Bacillus spp.: as plant growth-promoting bacteria","author":"Sansinenea","year":"2019"},{"key":"10.1016\/j.heliyon.2020.e04734_bib33","series-title":"Bacilli and Agrobiotechnology: Phytostimulation and Biocontrol","first-page":"81","article-title":"Plant growth promotion by ACC deaminase-producing Bacilli under salt stress conditions","author":"Santoyo","year":"2019"},{"key":"10.1016\/j.heliyon.2020.e04734_bib34","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1590\/S1517-83822004000100020","article-title":"Assessing in vitro solubilization potential of different zinc solubilizing bacterial (ZSB) isolates","volume":"35","author":"Saravanan","year":"2004","journal-title":"Braz. J. Microbiol."},{"issue":"2","key":"10.1016\/j.heliyon.2020.e04734_bib35","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/j.ab.2004.11.029","article-title":"A\u00a0nonenzymatic method to isolate genomic DNA from bacteria and actinomycete","volume":"337","author":"Sharma","year":"2005","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.heliyon.2020.e04734_bib36","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s12088-010-0045-6","article-title":"Antimicrobial activities of rhizobacterial strains of Pseudomonas and Bacillus strains isolated from rhizosphere soil of carnation (Dianthus caryophyllus cv. Sunrise)","volume":"50","author":"Sharma","year":"2010","journal-title":"Indian J. Microbiol."},{"key":"10.1016\/j.heliyon.2020.e04734_bib37","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s10725-013-9870-z","article-title":"Multifarious plant growth promoting characteristics of chickpea rhizosphere associated Bacilli help to suppress soil-borne pathogens","volume":"73","author":"Singh","year":"2014","journal-title":"Plant Growth Regul."},{"issue":"12","key":"10.1016\/j.heliyon.2020.e04734_bib38","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA6: molecular evolutionary genetics analysis version 6.0","volume":"30","author":"Tamura","year":"2013","journal-title":"Mol. Biol. Evol."},{"issue":"11","key":"10.1016\/j.heliyon.2020.e04734_bib39","doi-asserted-by":"crossref","first-page":"7292","DOI":"10.1128\/AEM.71.11.7292-7300.2005","article-title":"Paenibacillus polymyxa invades plant roots and forms biofilms","volume":"71","author":"Timmusk","year":"2005","journal-title":"Appl. Environ. Microbiol."},{"issue":"4","key":"10.1016\/j.heliyon.2020.e04734_bib40","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/s12088-014-0470-z","article-title":"Preferential promotion of Lycopersicon esculentum (tomato) growth by plant growth promoting bacteria associated with tomato","volume":"54","author":"Vaikuntapu","year":"2014","journal-title":"Indian J. Microbiol."},{"issue":"1","key":"10.1016\/j.heliyon.2020.e04734_bib41","first-page":"25","article-title":"Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction","volume":"5","author":"Versalovic","year":"1994","journal-title":"Methods Mol. Cell Biol."},{"issue":"3","key":"10.1016\/j.heliyon.2020.e04734_bib42","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1038\/nrmicro2960","article-title":"Sticking together: building a biofilm the Bacillus subtilis way","volume":"11","author":"Vlamakis","year":"2013","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.heliyon.2020.e04734_bib43","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/s11104-015-2743-7","article-title":"Root and bacterial secretions regulate the interaction between plants and PGPR leading to distinct plant growth promotion effects","volume":"401","author":"Zhou","year":"2016","journal-title":"Plant Soil"},{"key":"10.1016\/j.heliyon.2020.e04734_bib44","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.fbio.2013.12.003","article-title":"BOX-PCR and PCR-DGGE analysis for bacterial diversity of a naturally fermented functional food (Enzyme\u00ae)","volume":"5","author":"Zhu","year":"2014","journal-title":"Food Biosci."}],"container-title":["Heliyon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405844020315772?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405844020315772?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T17:46:30Z","timestamp":1762623990000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405844020315772"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8]]},"references-count":44,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2020,8]]}},"alternative-id":["S2405844020315772"],"URL":"https:\/\/doi.org\/10.1016\/j.heliyon.2020.e04734","relation":{},"ISSN":["2405-8440"],"issn-type":[{"value":"2405-8440","type":"print"}],"subject":[],"published":{"date-parts":[[2020,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Functional and molecular characterization of plant growth promoting Bacillus isolates from tomato rhizosphere","name":"articletitle","label":"Article Title"},{"value":"Heliyon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.heliyon.2020.e04734","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"e04734"}}