{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T02:58:23Z","timestamp":1793674703315,"version":"4.5.1"},"reference-count":52,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,1,1]],"date-time":"2024-01-01T00:00:00Z","timestamp":1704067200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,12,4]],"date-time":"2023-12-04T00:00:00Z","timestamp":1701648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008021","name":"Fujian University of Traditional Chinese Medicine","doi-asserted-by":"publisher","award":["X2021017-Key"],"award-info":[{"award-number":["X2021017-Key"]}],"id":[{"id":"10.13039\/501100008021","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Fujian Provincial Natural Science Foundation","doi-asserted-by":"publisher","award":["2021J05063"],"award-info":[{"award-number":["2021J05063"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cell.com","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Heliyon"],"published-print":{"date-parts":[[2024,1]]},"DOI":"10.1016\/j.heliyon.2023.e23402","type":"journal-article","created":{"date-parts":[[2023,12,9]],"date-time":"2023-12-09T18:23:51Z","timestamp":1702146231000},"page":"e23402","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":7,"title":["Huoxin pill protects verapamil-induced zebrafish heart failure through inhibition of oxidative stress-triggered inflammation and apoptosis"],"prefix":"10.1016","volume":"10","author":[{"given":"Xianmei","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laifeng","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixin","family":"Qu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenghua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.heliyon.2023.e23402_bib1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.15420\/cfr.2016:25:2","article-title":"Global public health burden of heart failure","volume":"3","author":"Savarese","year":"2017","journal-title":"Card. Fail. Rev."},{"key":"10.1016\/j.heliyon.2023.e23402_bib2","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1002\/ejhf.762","article-title":"Association between enrolment in a heart failure quality registry and subsequent mortality-a nationwide cohort study","volume":"19","author":"Lund","year":"2017","journal-title":"Eur. J. Heart Fail."},{"key":"10.1016\/j.heliyon.2023.e23402_bib3","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1002\/ejhf.496","article-title":"Use of evidence-based therapy and survival in heart failure in Sweden 2003-2012","volume":"18","author":"Thorvaldsen","year":"2016","journal-title":"Eur. J. Heart Fail."},{"key":"10.1016\/j.heliyon.2023.e23402_bib4","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1038\/s41569-021-00566-9","article-title":"Towards precision medicine in heart failure","volume":"18","author":"Weldy","year":"2021","journal-title":"Nat. Rev. Cardiol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib5","doi-asserted-by":"crossref","first-page":"458","DOI":"10.3389\/fphar.2020.00458","article-title":"Danqi pill protects against heart failure post-acute myocardial infarction via HIF-1alpha\/PGC-1alpha mediated glucose metabolism pathway","volume":"11","author":"Zhang","year":"2020","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib6","doi-asserted-by":"crossref","first-page":"48","DOI":"10.3389\/fphys.2018.00048","article-title":"Analysis of microRNA expression profiles induced by Yiqifumai injection in rats with chronic heart failure","volume":"9","author":"Zhao","year":"2018","journal-title":"Front. Physiol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib7","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1007\/s11655-020-2862-8","article-title":"Huoxin pill attenuates cardiac fibrosis by suppressing TGF-\u03b21\/smad2\/3 pathway in isoproterenol-induced heart failure rats","volume":"27","author":"Peng","year":"2021","journal-title":"Chin. J. Integr. Med."},{"key":"10.1016\/j.heliyon.2023.e23402_bib8","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1080\/13880209.2018.1561726","article-title":"Effects of huoxin formula on the arterial functions of patients with coronary heart disease","volume":"57","author":"Xu","year":"2019","journal-title":"Pharm. Biol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib9","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1080\/13880209.2021.1964542","article-title":"Network pharmacology-based analysis in determining the mechanisms of Huoxin pill in protecting against myocardial infarction","volume":"59","author":"He","year":"2021","journal-title":"Pharmaceut. Biol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib10","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1007\/978-981-32-9421-9_7","article-title":"Protective effect of Ganoderma (lingzhi) on cardiovascular system","volume":"1182","author":"Meng","year":"2019","journal-title":"Adv. Exp. Med. Biol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib11","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.jgr.2014.03.001","article-title":"A review on the medicinal potentials of ginseng and ginsenosides on cardiovascular diseases","volume":"38","author":"Lee","year":"2014","journal-title":"J Ginseng Res"},{"key":"10.1016\/j.heliyon.2023.e23402_bib12","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1111\/bph.15473","article-title":"Zebrafish as a tractable model of human cardiovascular disease","volume":"179","author":"Bowley","year":"2022","journal-title":"Br. J. Pharmacol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib13","doi-asserted-by":"crossref","first-page":"e109","DOI":"10.1016\/j.ddtec.2012.01.005","article-title":"Zebrafish-based small molecule screens for novel cardiovascular drugs","volume":"10","author":"Novodvorsky","year":"2013","journal-title":"Drug Discov. Today Technol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib14","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/nature12111","article-title":"The zebrafish reference genome sequence and its relationship to the human genome","volume":"496","author":"Howe","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.heliyon.2023.e23402_bib15","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-018-33577-7","article-title":"Development of a rapid and economic in vivo electrocardiogram platform for cardiovascular drug assay and electrophysiology research in adult zebrafish","volume":"8","author":"Lin","year":"2018","journal-title":"Sci. Rep."},{"key":"10.1016\/j.heliyon.2023.e23402_bib16","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1146\/annurev-genet-110711-155646","article-title":"Uncovering the molecular and cellular mechanisms of heart development using the zebrafish","volume":"46","author":"Staudt","year":"2012","journal-title":"Annu. Rev. Genet."},{"key":"10.1016\/j.heliyon.2023.e23402_bib17","doi-asserted-by":"crossref","DOI":"10.1016\/j.compbiomed.2021.105045","article-title":"Quantitative measurements of zebrafish heartrate and heart rate variability: a survey between 1990-2020","volume":"142","author":"Ling","year":"2022","journal-title":"Comput. Biol. Med."},{"key":"10.1016\/j.heliyon.2023.e23402_bib18","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1089\/adt.2013.548","article-title":"Zebrafish heart failure models for the evaluation of chemical probes and drugs","volume":"11","author":"Huang","year":"2013","journal-title":"Assay Drug Dev. Technol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib19","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/s11010-017-3018-9","article-title":"Effect of empagliflozin on cardiac biomarkers in a zebrafish model of heart failure: clues to the EMPA-REG OUTCOME trial?","volume":"433","author":"Shi","year":"2017","journal-title":"Mol. Cell. Biochem."},{"key":"10.1016\/j.heliyon.2023.e23402_bib20","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1089\/zeb.2017.1546","article-title":"A zebrafish heart failure model for assessing therapeutic agents","volume":"15","author":"Zhu","year":"2018","journal-title":"Zebrafish"},{"key":"10.1016\/j.heliyon.2023.e23402_bib21","doi-asserted-by":"crossref","first-page":"330","DOI":"10.14348\/molcells.2014.0003","article-title":"Thymosin beta4 regulates cardiac valve formation via endothelial-mesenchymal transformation in zebrafish embryos","volume":"37","author":"Shin","year":"2014","journal-title":"Mol. Cell."},{"key":"10.1016\/j.heliyon.2023.e23402_bib22","doi-asserted-by":"crossref","DOI":"10.3389\/fphar.2022.909084","article-title":"Identification of key pharmacodynamic markers of American ginseng against heart failure based on metabolomics and zebrafish model","volume":"13","author":"Dong","year":"2022","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib23","article-title":"Shen-yuan-dan capsule attenuates verapamil-induced zebrafish heart failure and exerts antiapoptotic and anti-inflammatory effects via reactive oxygen species-induced NF-\u03baB pathway","volume":"12","author":"Li","year":"2021","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib24","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1002\/jat.3340","article-title":"Acetyl L-carnitine targets adenosine triphosphate synthase in protecting zebrafish embryos from toxicities induced by verapamil and ketamine: an in vivo assessment","volume":"37","author":"Guo","year":"2017","journal-title":"J. Appl. Toxicol. : JAT"},{"key":"10.1016\/j.heliyon.2023.e23402_bib25","doi-asserted-by":"crossref","first-page":"2414","DOI":"10.1161\/01.CIR.96.7.2414","article-title":"Right and left myocardial antioxidant responses during heart failure subsequent to myocardial infarction","volume":"96","author":"Hill","year":"1997","journal-title":"Circulation"},{"key":"10.1016\/j.heliyon.2023.e23402_bib26","doi-asserted-by":"crossref","first-page":"1536","DOI":"10.1161\/01.CIR.97.16.1536","article-title":"Elevated levels of 8-iso-prostaglandin F2alpha in pericardial fluid of patients with heart failure: a potential role for in vivo oxidant stress in ventricular dilatation and progression to heart failure","volume":"97","author":"Mallat","year":"1998","journal-title":"Circulation"},{"key":"10.1016\/j.heliyon.2023.e23402_bib27","first-page":"RA225","article-title":"Atrial fibrillation and electrical remodeling: the potential role of inflammation and oxidative stress","volume":"9","author":"Korantzopoulos","year":"2003","journal-title":"Med. Sci. Mon. Int. Med. J. Exp. Clin. Res."},{"key":"10.1016\/j.heliyon.2023.e23402_bib28","doi-asserted-by":"crossref","first-page":"2083","DOI":"10.1093\/eurheartj\/ehi350","article-title":"Inflammation in the genesis and perpetuation of atrial fibrillation","volume":"26","author":"Engelmann","year":"2005","journal-title":"Eur. Heart J."},{"key":"10.1016\/j.heliyon.2023.e23402_bib29","article-title":"Alpha-calcitonin gene-related peptide prevents pressure-overload induced heart failure: role of apoptosis and oxidative stress","volume":"7","author":"Kumar","year":"2019","journal-title":"Phys. Rep."},{"key":"10.1016\/j.heliyon.2023.e23402_bib30","doi-asserted-by":"crossref","first-page":"568","DOI":"10.3389\/fendo.2020.00568","article-title":"Diabetes and sarcopenic obesity: pathogenesis, diagnosis, and treatments","volume":"11","author":"Wang","year":"2020","journal-title":"Front. Endocrinol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib31","doi-asserted-by":"crossref","DOI":"10.1016\/j.jhazmat.2020.124050","article-title":"Amorphous silica nanoparticles induce inflammation via activation of NLRP3 inflammasome and HMGB1\/TLR4\/MYD88\/NF-kb signaling pathway in HUVEC cells","volume":"404","author":"Liu","year":"2021","journal-title":"J. Hazard Mater."},{"key":"10.1016\/j.heliyon.2023.e23402_bib32","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1007\/s10741-019-09875-1","article-title":"Various aspects of inflammation in heart failure","volume":"25","author":"Dutka","year":"2020","journal-title":"Heart Fail. Rev."},{"key":"10.1016\/j.heliyon.2023.e23402_bib33","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.3389\/fphys.2018.01056","article-title":"Inflammation in right ventricular failure: does it matter?","volume":"9","author":"Dewachter","year":"2018","journal-title":"Front. Physiol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib34","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0008889","article-title":"miRNAs may play a major role in the control of gene expression in key pathobiological processes in Chagas disease cardiomyopathy","volume":"14","author":"Laugier","year":"2020","journal-title":"PLoS Neglected Trop. Dis."},{"key":"10.1016\/j.heliyon.2023.e23402_bib35","first-page":"253","volume":"203","author":"Kimmel","year":"1995"},{"key":"10.1016\/j.heliyon.2023.e23402_bib36","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.heliyon.2023.e23402_bib37","doi-asserted-by":"crossref","first-page":"219","DOI":"10.3389\/fgene.2013.00219","article-title":"Genetics and epigenetics of arrhythmia and heart failure","volume":"4","author":"Duygu","year":"2013","journal-title":"Front. Genet."},{"key":"10.1016\/j.heliyon.2023.e23402_bib38","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/j.jgg.2012.07.004","article-title":"Reverse genetic approaches in zebrafish","volume":"39","author":"Huang","year":"2012","journal-title":"J Genet Genomics"},{"key":"10.1016\/j.heliyon.2023.e23402_bib39","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1242\/dmm.016170","article-title":"The zebrafish as a tool to identify novel therapies for human cardiovascular disease","volume":"7","author":"Asnani","year":"2014","journal-title":"Dis Model Mech"},{"key":"10.1016\/j.heliyon.2023.e23402_bib40","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/nrd1606","article-title":"In vivo drug discovery in the zebrafish","volume":"4","author":"Zon","year":"2005","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.heliyon.2023.e23402_bib41","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2021.662583","article-title":"Zebrafish heart failure models","volume":"9","author":"Narumanchi","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib42","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2020.111050","article-title":"Design, synthesis and biological evaluation of novel pyxinol derivatives with anti-heart failure activity","volume":"133","author":"Liu","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.heliyon.2023.e23402_bib43","doi-asserted-by":"crossref","DOI":"10.3390\/molecules26134102","article-title":"Antioxidant, anti-inflammatory and antithrombotic effects of ginsenoside compound K enriched extract derived from ginseng sprouts","volume":"26","author":"Baik","year":"2021","journal-title":"Molecules"},{"key":"10.1016\/j.heliyon.2023.e23402_bib44","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1038\/s41580-022-00456-z","article-title":"Defining roles of specific reactive oxygen species (ROS) in cell biology and physiology","volume":"23","author":"Sies","year":"2022","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"10.1016\/j.heliyon.2023.e23402_bib45","doi-asserted-by":"crossref","first-page":"11053","DOI":"10.1111\/jcmm.17028","article-title":"Huoxin pill prevents acute myocardial ischaemia injury via inhibition of Wnt\/\u03b2-catenin signaling","volume":"25","author":"Wang","year":"2021","journal-title":"J. Cell Mol. Med."},{"key":"10.1016\/j.heliyon.2023.e23402_bib46","doi-asserted-by":"crossref","DOI":"10.3390\/nu11092090","article-title":"Oxidative stress in cardiovascular diseases: still a therapeutic target?","volume":"11","author":"Senoner","year":"2019","journal-title":"Nutrients"},{"key":"10.1016\/j.heliyon.2023.e23402_bib47","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.cell.2010.03.006","article-title":"Inflammation 2010: new adventures of an old flame","volume":"140","author":"Medzhitov","year":"2010","journal-title":"Cell"},{"key":"10.1016\/j.heliyon.2023.e23402_bib48","doi-asserted-by":"crossref","first-page":"6731","DOI":"10.1038\/sj.onc.1209936","article-title":"Mutual cross-talk between reactive oxygen species and nuclear factor-kappa B: molecular basis and biological significance","volume":"25","author":"Bubici","year":"2006","journal-title":"Oncogene"},{"key":"10.1016\/j.heliyon.2023.e23402_bib49","doi-asserted-by":"crossref","first-page":"28451","DOI":"10.1074\/jbc.M102698200","article-title":"Activation of NF-kappa B via the Ikappa B kinase complex is both essential and sufficient for proinflammatory gene expression in primary endothelial cells","volume":"276","author":"Denk","year":"2001","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.heliyon.2023.e23402_bib50","doi-asserted-by":"crossref","first-page":"3421","DOI":"10.1182\/blood.V89.9.3421","article-title":"Activation of the NF-kappaB pathway by inflammatory stimuli in human neutrophils","volume":"89","author":"McDonald","year":"1997","journal-title":"Blood"},{"key":"10.1016\/j.heliyon.2023.e23402_bib51","doi-asserted-by":"crossref","first-page":"25542","DOI":"10.1074\/jbc.M303760200","article-title":"Regulation of Bcl-xL expression by H2O2 in cardiac myocytes","volume":"278","author":"Valks","year":"2003","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.heliyon.2023.e23402_bib52","doi-asserted-by":"crossref","DOI":"10.1016\/j.jep.2021.114061","article-title":"Huoxin Pill inhibits isoproterenol-induced transdifferentiation and collagen synthesis in cardiac fibroblasts through the TGF-\u03b2\/Smads pathway","volume":"275","author":"Peng","year":"2021","journal-title":"J. Ethnopharmacol."}],"container-title":["Heliyon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405844023106104?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2405844023106104?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T04:46:12Z","timestamp":1790657172000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2405844023106104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1]]},"references-count":52,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["S2405844023106104"],"URL":"https:\/\/doi.org\/10.1016\/j.heliyon.2023.e23402","relation":{},"ISSN":["2405-8440"],"issn-type":[{"value":"2405-8440","type":"print"}],"subject":[],"published":{"date-parts":[[2024,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Huoxin pill protects verapamil-induced zebrafish heart failure through inhibition of oxidative stress-triggered inflammation and apoptosis","name":"articletitle","label":"Article Title"},{"value":"Heliyon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.heliyon.2023.e23402","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"e23402"}}